Functionalization of wool with L‐cysteine: Process characterization and assessment of antimicrobial activity and cytotoxicity
Abstract
This investigation reports a new biotechnological process that uses L‐cysteine (L‐Cys) which provides a permanent, nontoxic and effective antimicrobial effect over wool‐based materials. This process is simple and carried out via widespread exhausting methods. Typically, wool fabrics are incubated with L‐Cys for 50 min at 60°C in a pH 4.8 acetate buffer solution 25 mM, under mild agitation to give a good absorption rate. The minimal inhibitory concentration (MIC) of L‐Cys was evaluated by the NCCLS M07‐A6 standard method, and the results showed a good antibacterial activity against S. aureus and K. pneumoniae within the range of 6.0 × 10−3 − 4.8 × 10−2 g/mL [MIC 0.6% (w/v)] and 6.0 × 10−3 − 4.8 × 10−2 g/mL [MIC 0.6% (w/v)], respectively. In addition, the antimicrobial activity of the functionalized wool was assessed by the international standard JIS 1902‐2002 showing a good inhibition of bacterial growth for an L‐Cys concentration of 1% over the weight of fabric, both against Staphylococcus aureus and Klebsiella pneumoniae. Moreover, the biocidal mechanism was found to be related with the increase of sulfhydryl's groups onto wool fibers, which were quantified by the Ellman's reagent (5,5′‐Dithio‐bis(2‐nitrobenzoic acid) method. The new process is easy to perform, non toxic, preserve wool quality and is a novel biomimetic approach that uses antimicrobial amino acids and may open new avenues for the design of biomedical textiles with a broad range of applications in healthcare. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Number of times cited according to CrossRef: 13
- QingBo Xu, XiaTing Ke, DongRong Cai, YanYan Zhang, FeiYa Fu, Takeshi Endo and XiangDong Liu, Silver-based, single-sided antibacterial cotton fabrics with improved durability via an l-cysteine binding effect, Cellulose, 10.1007/s10570-018-1689-3, 25, 3, (2129-2141), (2018).
- Zhuang Du, Bolin Ji and Kelu Yan, Recycling keratin polypeptides for anti-felting treatment of wool based on L-cysteine pretreatment, Journal of Cleaner Production, 10.1016/j.jclepro.2018.02.196, 183, (810-817), (2018).
- Frederico Nogueira, Luíza Granadeiro, Claudia Mouro and Isabel C. Gouveia, Antimicrobial and antioxidant surface modification toward a new silk-fibroin (SF)- l -Cysteine material for skin disease management, Applied Surface Science, 10.1016/j.apsusc.2015.12.174, 364, (552-559), (2016).
- Cláudia Mouro and Isabel C. Gouveia, Antimicrobial functionalization of wool: assessment of the effect of Cecropin-B and [Ala5]-Tritrp7 antimicrobial peptides, The Journal of The Textile Institute, 107, 12, (1575), (2016).
- Ana P. Gomes, João F. Mano, João A. Queiroz and Isabel C. Gouveia, Layer-by-Layer Assembly for Biofunctionalization of Cellulosic Fibers with Emergent Antimicrobial Agents, Cellulose Chemistry and Properties: Fibers, Nanocelluloses and Advanced Materials, 10.1007/12_2015_318, (225-240), (2015).
- A.P. Gomes, J.F. Mano, J.A. Queiroz and I.C. Gouveia, Incorporation of antimicrobial peptides on functionalized cotton gauzes for medical applications, Carbohydrate Polymers, 10.1016/j.carbpol.2015.03.089, 127, (451-461), (2015).
- Ana P. Gomes, João F. Mano, João A. Queiroz and Isabel C. Gouveia, New biomaterial based on cotton with incorporated Biomolecules, Journal of Applied Polymer Science, 131, 15, (2014).
- Marta Pedrosa, Cláudia Mouro, Frederico Nogueira, Joana Vaz and Isabel Gouveia, Comparison of the antibacterial activity of modified‐cotton with magainin I and LL‐37 with potential as wound‐dressings, Journal of Applied Polymer Science, 131, 21, (2014).
- Zahra Mohammad Davoudi, Ahmad Esmaielzadeh Kandjani, Anand I. Bhatt, Ilias L. Kyratzis, Anthony P. O'Mullane and Vipul Bansal, Hybrid Antibacterial Fabrics with Extremely High Aspect Ratio Ag/AgTCNQ Nanowires, Advanced Functional Materials, 24, 8, (1047-1053), (2013).
- Frederico Nogueira, Joana Vaz, Claudia Mouro, Erhan Piskin and Isabel Gouveia, Covalent modification of cellulosic-based textiles: A new strategy to obtain antimicrobial properties, Biotechnology and Bioprocess Engineering, 10.1007/s12257-013-0498-7, 19, 3, (526-533), (2014).
- Jiajia Shen, Pu Gao and Hui Ma, The effect of tris(2-carboxyethyl)phosphine on the dyeing of wool fabrics with natural dyes, Dyes and Pigments, 108, (70), (2014).
- Cláudia Mouro, Marta Pedrosa, Joana Vaz and Isabel Gouveia, Two Surface Activation Strategies to Functionalize Cotton Fibers with Cys-LC-LL-37 Antibacterial Peptide, AATCC Journal of Research, 1, 6, (27), (2014).
- Estela Caldeira, Erhan Piskin, Luiza Granadeiro, Filomena Silva and Isabel C. Gouveia, Biofunctionalization of cellulosic fibres with l-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniae, Journal of Biotechnology, 10.1016/j.jbiotec.2013.10.021, 168, 4, (426-435), (2013).
