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Lipid Bilayer Membrane of Egg‐PC Liposome Evaluated as Chemical Reaction Field with Picosecond Time‐Resolved Fluorescence Spectroscopy

Department of Chemistry, Faculty of Science, Gakushuin University, 1‐5‐1 Mejiro, Toshima‐ku, Tokyo 171‐8588 (Japan), Fax: (+81)‐3‐5992‐1029

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Prof. Koichi Iwata

Corresponding Author

koichi.iwata@gakushuin.ac.jp

Department of Chemistry, Faculty of Science, Gakushuin University, 1‐5‐1 Mejiro, Toshima‐ku, Tokyo 171‐8588 (Japan), Fax: (+81)‐3‐5992‐1029

Department of Chemistry, Faculty of Science, Gakushuin University, 1‐5‐1 Mejiro, Toshima‐ku, Tokyo 171‐8588 (Japan), Fax: (+81)‐3‐5992‐1029Search for more papers by this author

Yuki Nojima

Department of Chemistry, Faculty of Science, Gakushuin University, 1‐5‐1 Mejiro, Toshima‐ku, Tokyo 171‐8588 (Japan), Fax: (+81)‐3‐5992‐1029

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Prof. Koichi Iwata

Corresponding Author

koichi.iwata@gakushuin.ac.jp

Department of Chemistry, Faculty of Science, Gakushuin University, 1‐5‐1 Mejiro, Toshima‐ku, Tokyo 171‐8588 (Japan), Fax: (+81)‐3‐5992‐1029

Department of Chemistry, Faculty of Science, Gakushuin University, 1‐5‐1 Mejiro, Toshima‐ku, Tokyo 171‐8588 (Japan), Fax: (+81)‐3‐5992‐1029Search for more papers by this author

First published: 09 May 2011

https://doi.org/10.1002/asia.201100143

Citations: 5

Abstract

Viscosity inside the lipid bilayer of egg‐PC (egg yolk phosphatidylcholine) liposome with a diameter of 100 nm is examined with picosecond time‐resolved fluorescence spectroscopy. The viscosity is estimated independently from the photoisomerization rate and from the rotational relaxation time of the first excited singlet state of trans‐stilbene solubilized within the lipid bilayer. The presence of two solvation environments within the bilayer is suggested from both of the methods. One environment is 50 to 100 times more viscous than the other. The use of trans‐stilbene as a probe provides valuable information on the solvation environments inside the lipid bilayer of liposome, which serves as a field for a number of biochemical reactions.