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Article

Low‐frequency Raman spectra of lysozyme

L. Genzel

Max‐Planck Institut für Festkörperforschung, Stuttgart, Federal Republic of Germany

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F. Keilmann

Max‐Planck Institut für Festkörperforschung, Stuttgart, Federal Republic of Germany

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T. P. Martin

Max‐Planck Institut für Festkörperforschung, Stuttgart, Federal Republic of Germany

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G. Wintreling

Max‐Planck Institut für Festkörperforschung, Stuttgart, Federal Republic of Germany

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Y. Yacoby

Max‐Planck Institut für Festkörperforschung, Stuttgart, Federal Republic of Germany

Racah Institute of Physics, Hebrew university, Jerusalem. Israel.Search for more papers by this author
H. Fröhlich

Department of Electrical Engineering, University of Salford, Salford, England

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Marvin W. Makinen

Laboratory of Molecular Biophysics, Department of Zoology, Oxford University, Oxford, England

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First published: January 1976
Cited by: 100

Abstract

New techniques in laser Raman spectroscopy are used to obtain spectra of aqueous solutions of lysozylme for frequency shifts as small as 5 cm−1. In addition, Raman measurements are made on two crystalline forms of hen egg white lysozyme. The spectra obtained from the solution and from the crystal are found to be similar for frequencies above 100 cm−1. However, a low‐frequency band at 25 cm−1 observed in crystalline lysozyme is not found in the solution, indicating that this band cannot be attributed to an internal molecular vibration.

Number of times cited: 100

  • , New Method to Study the Vibrational Modes of Biomolecules in the Terahertz Range Based on a Single-Stage Raman Spectrometer, ACS Omega, 2, 3, (1232), (2017).
  • , Low-Frequency Vibrational Modes of Poly(glycolic acid) and Thermal Expansion of Crystal Lattice Assigned On the Basis of DFT-Spectral Simulation Aided with a Fragment Method, The Journal of Physical Chemistry B, 10.1021/acs.jpcb.6b11304, 121, 5, (1128-1138), (2017).
  • , Terahertz mechanical vibrations in lysozyme: Raman spectroscopy vs modal analysis, Journal of Molecular Structure, 10.1016/j.molstruc.2017.02.099, 1139, (222-230), (2017).
  • , Theoretical Insights into Sub-Terahertz Acoustic Vibrations of Proteins Measured in Single-Molecule Experiments, The Journal of Physical Chemistry Letters, 7, 24, (5128), (2016).
  • , Fingerprints of Conformational States of Human Hsp70 at Sub-THz Frequencies, ACS Omega, 1, 6, (1067), (2016).
  • , Classical investigation of long-range coherence in biological systems, Chaos: An Interdisciplinary Journal of Nonlinear Science, 26, 12, (123116), (2016).
  • , Possible role of electrodynamic interactions in long-distance biomolecular recognition, Physical Review E, 91, 5, (2015).
  • , Experimental detection of long-distance interactions between biomolecules through their diffusion behavior: Numerical study, Physical Review E, 90, 2, (2014).
  • , Terahertz Spectroscopic Insight into the Structure–Function Relation in Protein Molecules, Terahertz Biomedical Science and Technology, 10.1201/b17060-13, (173-194), (2014).
  • , Quantum Mechanical Interpretation of Intermolecular Vibrational Modes of Crystalline Poly-( R )-3-Hydroxybutyrate Observed in Low-Frequency Raman and Terahertz Spectra , The Journal of Physical Chemistry B, 10.1021/jp309704k, 117, 7, (2180-2187), (2013).
  • , Low-temperature molecular dynamics simulations of horse heart cytochrome c and comparison with inelastic neutron scattering data, European Biophysics Journal, 10.1007/s00249-012-0874-9, 42, 4, (291-300), (2012).
  • , Terahertz Pioneer: Fritz Keilmann - "RF Biophysics: From strong field to near field", IEEE Transactions on Terahertz Science and Technology, 3, 5, (506), (2013).
  • , Analysis of cold denaturation mechanism of β‐lactoglobulin and comparison with thermal denaturation from Raman spectroscopy investigations, Journal of Raman Spectroscopy, 43, 1, (16-23), (2011).
  • , Coherent dynamics and ultrafast excited state relaxation of blue copper protein; plastocyanin, Physical Chemistry Chemical Physics, 12, 23, (6067), (2010).
  • , Solvent-induced backbone fluctuations and the collective librational dynamics of lysozyme studied by terahertz spectroscopy, Physical Review E, 10.1103/PhysRevE.81.031915, 81, 3, (2010).
  • , Time-Domain Coherent Anti-Stokes Raman Scattering Signal Detection for Terahertz Vibrational Spectroscopy Using Chirped Femtosecond Pulses, Applied Physics Express, 3, 7, (072401), (2010).
  • , Fractal properties of lysozyme: A neutron scattering study, Physical Review E, 79, 3, (2009).
  • , Low-frequency vibrational properties of lysozyme in sugar aqueous solutions: A Raman scattering and molecular dynamics simulation study, The Journal of Chemical Physics, 131, 24, (245103), (2009).
  • , Dynamical implications of Viral Tiling Theory, Journal of Theoretical Biology, 252, 2, (357), (2008).
  • , Low-frequency vibrational motions in proteins: Physical mechanisms and effect on functioning, The European Physical Journal B, 65, 3, (419), (2008).
  • , Study on the influence of isotope exchange of hydrogen with deuterium on the vibrational spectrum of lysozyme by inelastic neutron scattering, Crystallography Reports, 52, 5, (838), (2007).
  • , Reactions of Aldehydes and Ketones and Their Derivatives, Organic Reaction Mechanisms, (1-27), (2007).
  • , Inelastic Incoherent Neutron Scattering in Some Proteins, Ferroelectrics, 348, 1, (154), (2007).
  • , References, Advances in Chemical Physics, (233-249), (2007).
  • , Laser Raman spectroscopy of the effect of solvent on the low-frequency oscillations of organic molecules, Laser Physics, 17, 9, (1133), (2007).
  • , Four‐photon Rayleigh‐wing spectroscopy of α‐chymotrypsin protein in water, Journal of Raman Spectroscopy, 37, 6, (689-692), (2006).
  • , ON THE DAMPING OF THE FLUCTUATIONS OF ATOMIC GROUPS IN WATER ENVIRONMENT, Fluctuation and Noise Letters, 06, 02, (L133), (2006).
  • , Coherent four-photon spectroscopy of low-frequency molecular librations in a liquid, Uspekhi Fizicheskih Nauk, 176, 8, (883), (2006).
  • , Raman scattering of acoustical modes of silicon nanoparticles embedded in silica matrix, Journal of Raman Spectroscopy, 37, 1‐3, (161-165), (2006).
  • 13th European Microwave Conference, 1983 Nurnberg, Germany 13th European Microwave Conference, 1983 IEEE , (2006). The Non-Thermal Effect of Millimeter Wave Radiation on the Puffing of Giant Chromosomes , (2006). 859 864 4131998 , 10.1109/EUMA.1983.333298 http://ieeexplore.ieee.org/document/4131998/
  • , Characterization of amino acids using Raman spectroscopy, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 61, 7, (1585), (2005).
  • , Vibrational density of states of hen egg white lysozyme, Journal of Experimental and Theoretical Physics Letters, 82, 1, (30), (2005).
  • 2005 7th International Conference Transparent Optical Networks, 2005. Barcelona, Catlonia, Spain July 3-7, 2005 Proceedings of 2005 7th International Conference Transparent Optical Networks, 2005. IEEE , (2005). 2 0-7803-9236-1 Raman scattering technique in characterisation of glasses containing silicon nanoparticles for optoelectronics 245 249 , 10.1109/ICTON.2005.1506144 http://ieeexplore.ieee.org/document/1506144/
  • , Neutron scattering and molecular dynamics simulation: a conjugate approach to investigate the dynamics of electron transfer proteins, Journal of Physics: Condensed Matter, 10.1088/0953-8984/16/6/R01, 16, 6, (R83-R110), (2004).
  • , Quantifying anisotropic solute transport in protein crystals using 3‐D laser scanning confocal microscopy visualization, Biotechnology and Bioengineering, 86, 4, (389-398), (2004).
  • , Elastic vibrations of spheroidal nanometric particles, Journal of Physics: Condensed Matter, 15, 44, (7481), (2003).
  • , Polarization-selective femtosecond Raman spectroscopy of low-frequency motions in hydrated protein films, Chemical Physics Letters, 376, 1-2, (20), (2003).
  • , Low-Frequency Modes of Peptides and Globular Proteins in Solution Observed by Ultrafast OHD-RIKES Spectroscopy, Biophysical Journal, 85, 3, (1903), (2003).
  • , Boson peak and terahertz frequency dynamics of vitreous silica, Reports on Progress in Physics, 65, 8, (1195), (2002).
  • , Far-Infrared Absorption Measurements of Polypeptides and Cytochromecby THz Radiation, Bulletin of the Chemical Society of Japan, 75, 5, (1083), (2002).
  • , Effects of solvent damping on side chain and backbone contributions to the protein boson peak, The Journal of Chemical Physics, 115, 3, (1607), (2001).
  • , Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by Raman and IR spectroscopies, Biopolymers, 53, 4, (342-349), (2000).
  • , Low frequency vibrational anomalies in hydrated copper azurin: A neutron scattering and MD simulation study, Journal of Molecular Liquids, 84, 1, (3), (2000).
  • , Glasslike dynamical behavior of the plastocyanin hydration water, Physical Review E, 62, 3, (3991), (2000).
  • , Far-Infrared Emission by Boson Peak Vibrations in a Globular Protein, Physical Review Letters, 82, 14, (2987), (1999).
  • , Origin of the low-frequency modes of globular proteins, Physical Review E, 60, 4, (4664), (1999).
  • , Neutron scattering evidence of a boson peak in protein hydration water, Physical Review E, 60, 3, (R2476), (1999).
  • , Low energy dynamics of globular proteins studied by inelastic neutron scattering, Journal of Physics and Chemistry of Solids, 60, 8-9, (1285), (1999).
  • , Low-Frequency Raman Spectra of Lysozyme Crystals and Oriented DNA Films: Dynamics of Crystal Water, Biophysical Journal, 74, 3, (1533), (1998).
  • , Glassylike low-frequency dynamics of globular proteins, Physical Review E, 58, 1, (845), (1998).
  • , Low-frequency vibrational spectroscopy of amides and urethanes, Russian Chemical Reviews, 66, 5, (375), (1997).
  • , Influence of perturbations on the lattice spectra of crystalline amino acids, Journal of Russian Laser Research, 17, 2, (119), (1996).
  • , Diffuse scattering in protein crystallography, Quarterly Reviews of Biophysics, 28, 02, (131), (1995).
  • , [17] Raman spectroscopy of DNA and proteins, Biochemical Spectroscopy, 10.1016/0076-6879(95)46019-5, (389-416), (1995).
  • , Normal mode analysis of oligomeric proteins: Reduction of the memory requirement by consideration of rigid geometry and molecular symmetry, Journal of Computational Chemistry, 15, 8, (820-837), (2004).
  • , Biophysical aspects of neutron scattering from vibrational modes of proteins, Progress in Biophysics and Molecular Biology, 57, 3, (129), (1992).
  • , The vibrational normal modes of β-barrels in an IgG antibody molecule, Biophysical Chemistry, 44, 1, (29), (1992).
  • , Protein dynamics: comparison of simulations with inelastic neutron scattering experiments, Quarterly Reviews of Biophysics, 24, 03, (227), (1991).
  • , Effect of urey–bradley–shimanouchi force field on the harmonic dynamics of proteins, "Proteins: Structure, Function, and Bioinformatics", 11, 2, (120-132), (2004).
  • , Molecular dynamics study of the depolymerization reaction in simple polymers, Chemical Physics Letters, 171, 5-6, (499), (1990).
  • , Dynamical structure of carboxypeptidase A, Journal of Molecular Biology, 207, 1, (201), (1989).
  • , Low-frequency collective motion in biomacromolecules and its biological functions, Biophysical Chemistry, 30, 1, (3), (1988).
  • , Collective motion in DNA and its role in drug intercalation, Biopolymers, 27, 11, (1795-1815), (2004).
  • , Biological functions of low‐frequency vibrations (phonons). 7. The impetus for DNA to accommodate intercalators, British Polymer Journal, 20, 2, (143-148), (2007).
  • , The biological functions of low‐frequency vibrations (phonons). VI. A possible dynamic mechanism of allosteric transition in antibody molecules, Biopolymers, 26, 2, (285-295), (2004).
  • , The growth of large single crystals of lysozyme, Biopolymers, 25, 2, (337-350), (2004).
  • , Origin of low-frequency motions in biological macromolecules, Biophysical Chemistry, 25, 2, (105), (1986).
  • , The biological functions of low-frequency vibrations (phonons), Biophysical Chemistry, 22, 3, (219), (1985).
  • , Protein normal-mode dynamics: Trypsin inhibitor, crambin, ribonuclease and lysozyme, Journal of Molecular Biology, 181, 3, (423), (1985).
  • , The biological functions of low-frequency vibrations (phonons), Biophysical Chemistry, 20, 1-2, (61), (1984).
  • , Possible observation of a defect resonance in DNA, Biopolymers, 22, 9, (2045-2060), (2004).
  • , Low-temperature and time-resolved protein crystallography using synchrotron radiation, Nuclear Instruments and Methods in Physics Research, 208, 1-3, (523), (1983).
  • , The normal modes of a protein: Native bovine pancreatic trypsin inhibitor, International Journal of Quantum Chemistry, 24, S10, (181-199), (2009).
  • , Intramolecular low‐frequency vibrations in lysozyme by neutron time‐of‐flight spectroscopy, Biopolymers, 21, 1, (43-50), (2004).
  • , A Collective Dynamics in Metabolically Active Cells, Physica Scripta, 26, 3, (232), (1982).
  • , Dynamics of Davydov solitons, Physical Review A, 10.1103/PhysRevA.26.578, 26, 1, (578-595), (1982).
  • , Low‐frequency modes in the Raman spectra of proteins, Biopolymers, 21, 7, (1469-1472), (2004).
  • , The Vibrational Structure of Davydov Solitons, Physica Scripta, 25, 5, (651), (1982).
  • , References, Biochemical Applications of Raman and Resonance Raman Spectroscopes, 10.1016/B978-0-12-159650-7.50013-4, (238-252), (1982).
  • , Low‐frequency modes in the raman spectrum of DNA, Biopolymers, 20, 1, (243-247), (2004).
  • , The Internal Dynamics of Globular Protein, Critical Reviews in Biochemistry, 9, 4, (293), (1981).
  • , QUANTITATIVE DETERMINATION OF THE SECONDARY STRUCTURE OF PROTEINS IN SOLUTION BY LASER RAMAN SCATTERING, Diffraction and Related Studies, 10.1016/B978-1-4832-8364-7.50061-1, (45-58), (1981).
  • , The Anatomy and Taxonomy of Protein Structure, Advances in Protein Chemistry Volume 34, 10.1016/S0065-3233(08)60520-3, (167-339), (1981).
  • , Laser-Raman spectroscopy of living cells, Physics Reports, 60, 4, (201), (1980).
  • , The Biological Effects of Microwaves and Related Questions, Advances in Electronics and Electron Physics Volume 53, 10.1016/S0065-2539(08)60259-0, (85-152), (1980).
  • , Possible role of protein in photosynthetic electron transfer, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 589, 1, (71), (1980).
  • , The low‐frequency Raman spectrum of an antibody molecule: bovine IgG, Biopolymers, 18, 12, (3121-3123), (2004).
  • , [18] Low frequency vibrations and the dynamics of proteins and polypeptides, Enzyme Structure Part H, 10.1016/0076-6879(79)61020-0, (425-458), (1979).
  • , Application of laser Raman and infrared spectroscopy to the analysis of membrane structure, Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 559, 2-3, (153), (1979).
  • , Mean‐square amplitudes of the longitudinal vibrational of helical polymers, Biopolymers, 18, 4, (747-755), (2004).
  • , Far‐infrared spectrum of crystalline lysozyme, Biopolymers, 18, 3, (507-516), (2004).
  • , Dynamic information from protein crystallography, Journal of Molecular Biology, 130, 3, (231), (1979).
  • , Laser raman spectroscopy of acid phosphatase from rat liver after neuraminidase treatment, Biochimica et Biophysica Acta (BBA) - Protein Structure, 578, 1, (116), (1979).
  • , The “Van Der Waals interaction” between loose structures, Physics Letters A, 68, 1, (131), (1978).
  • , Coherent Electric Vibrations in Biological Systems and the Cancer Problem, IEEE Transactions on Microwave Theory and Techniques, 26, 8, (613), (1978).
  • , Use of Laser Raman Techniques in the Study of Polymers, Characterization of Metal and Polymer Surfaces, 10.1016/B978-0-12-442102-8.50017-6, (207-240), (1977).
  • , Nonsteady hydrodynamics of biopolymer motions, The Journal of Chemical Physics, 66, 4, (1452), (1977).
  • , The hinge-bending mode in lysozyme, Nature, 262, 5566, (325), (1976).
  • , Optical measurements of long-range protein vibrations, Nature Communications, 10.1038/ncomms4076, 5, (2014).
  • , Terahertz underdamped vibrational motion governs protein-ligand binding in solution, Nature Communications, 10.1038/ncomms4999, 5, (2014).