Article
Disruption of native and recombinant Escherichia coli in a high‐pressure homogenizer
Abstract
The disruption of native and recombinant strains of Escherichia coli was studied using a high‐pressure homogenizer (Microfluidizer). The cells were grown in both batch and continuous fermentations. Cell suspensions ranging from 4 to 175 g dry wt/L were investigated at disruption pressures ranging from 30–95 MPa and at up to five passes. For both types of cells, the fraction of cells disrupted was dependent on the growth rate and concentration of the cells, the disruption pressure, and the number of passes through the disrupter. A model is presented that correlates the fractional disruption with these operating variables. The recombinant strain disrupted more readily than the native strain; 95 to 98% disruption of the former was achieved in two to three passes at a pressure of 95 MPa.
Citing Literature
Number of times cited: 65
- Florencia Eberhardt, Andres Aguirre, Luciana Paoletti, Guillermo Hails, Mauricio Braia, Pablo Ravasi, Salvador Peiru and Hugo G. Menzella, Pilot-scale process development for low-cost production of a thermostable biodiesel refining enzyme in Escherichia coli, Bioprocess and Biosystems Engineering, (2018).
- Anurag S. Rathore, Nikhil Kateja and Harshit Agarwal, Continuous Downstream Processing for Production of Biotech Therapeutics, Continuous Biomanufacturing ‐ Innovative Technologies and Methods, (259-288), (2017).
- Mohammed Shehadul Islam, Aditya Aryasomayajula and Ponnambalam Selvaganapathy, A Review on Macroscale and Microscale Cell Lysis Methods, Micromachines, 8, 3, (83), (2017).
- Cornelia Walther and Astrid Dürauer, Microscale disruption of microorganisms for parallelized process development, Biotechnology Journal, 12, 7, (2017).
- Li Xie, Akihiko Terada and Masaaki Hosomi, Disentangling the multiple effects of a novel high pressure jet device upon bacterial cell disruption, Chemical Engineering Journal, 323, (105), (2017).
- Youping Xie, Shih-Hsin Ho, Ching-Nen Nathan Chen, Chun-Yen Chen, Keju Jing, I-Son Ng, Jianfeng Chen, Jo-Shu Chang and Yinghua Lu, Disruption of thermo-tolerant Desmodesmus sp. F51 in high pressure homogenization as a prelude to carotenoids extraction, Biochemical Engineering Journal, 109, (243), (2016).
- Leonard E.N. Ekpeni, Khaled Y. Benyounis, Joseph Stokes and Abdul G. Olabi, Improving and optimizing protein concentration yield from homogenized baker's yeast at different ratios of buffer solution, International Journal of Hydrogen Energy, 41, 37, (16415), (2016).
- Li Xie, Qian Bao, Akihiko Terada and Masaaki Hosomi, Single-cell analysis of the disruption of bacteria with a high-pressure jet device: An application of atomic force microscopy, Chemical Engineering Journal, 306, (1099), (2016).
- Nan Wu, Tetsuya Kamioka and Yutaka Kuroda, A novel screening system based on VanX‐mediated autolysis—Application to Gaussia luciferase, Biotechnology and Bioengineering, 113, 7, (1413-1420), (2016).
- H. Delmas and L. Barthe, Ultrasonic mixing, homogenization, and emulsification in food processing and other applications, Power Ultrasonics, 10.1016/B978-1-78242-028-6.00025-9, (757-791), (2015).
- Inga Gerlach, Carl-Fredrik Mandenius and Volker C. Hass, Operator training simulation for integrating cultivation and homogenisation in protein production, Biotechnology Reports, 6, (91), (2015).
- Anurag S. Rathore, Harshit Agarwal, Abhishek Kumar Sharma, Mili Pathak and S. Muthukumar, Continuous Processing for Production of Biopharmaceuticals, Preparative Biochemistry and Biotechnology, 10.1080/10826068.2014.985834, 45, 8, (836-849), (2015).
- Li Xie, Qian Bao, Toshikazu Suenaga, Hiroyuki Yoshino, Akihiko Terada and Masaaki Hosomi, Identification of a predominant effect on bacterial cell disruption and released organic matters by a high-pressure jet device, Biochemical Engineering Journal, 101, (220), (2015).
- Ramya Natarajan, Wei Ming Russell Ang, Xue Chen, Michael Voigtmann and Raymond Lau, Lipid releasing characteristics of microalgae species through continuous ultrasonication, Bioresource Technology, 10.1016/j.biortech.2014.01.146, 158, (7-11), (2014).
- Alois Jungbauer, Continuous downstream processing of biopharmaceuticals, Trends in Biotechnology, 10.1016/j.tibtech.2013.05.011, 31, 8, (479-492), (2013).
- Erin M. Spiden, Peter J. Scales, Sandra E. Kentish and Gregory J.O. Martin, Critical analysis of quantitative indicators of cell disruption applied to Saccharomyces cerevisiae processed with an industrial high pressure homogenizer, Biochemical Engineering Journal, 70, (120), (2013).
- Qiang Li, Gareth J. Mannall, Shaukat Ali and Mike Hoare, An ultra scale‐down approach to study the interaction of fermentation, homogenization, and centrifugation for antibody fragment recovery from rec E. coli, Biotechnology and Bioengineering, 110, 8, (2150-2160), (2013).
- Huzaifa S. Choonia, Supriya D. Saptarshi and S. S. Lele, RELEASE OF INTRACELLULAR β-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS AND L-ASPARAGINASE FROM PECTOBACTERIUM CAROTOVORUM BY HIGH-PRESSURE HOMOGENIZATION , Chemical Engineering Communications, 10.1080/00986445.2012.751376, 200, 11, (1415-1424), (2013).
- Mitra Mohammadi, Mohd Ali Hassan, Lai-Yee Phang, Yoshihito Shirai, Hasfalina Che Man and Hidayah Ariffin, Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill, Journal of Cleaner Production, 37, (353), (2012).
- Nalin Samarasinghe, Sandun Fernando, Ronald Lacey and William Brock Faulkner, Algal cell rupture using high pressure homogenization as a prelude to oil extraction, Renewable Energy, 48, (300), (2012).
- Yuxuan Zhang, Panyue Zhang, Guangming Zhang, Weifang Ma, Hao Wu and Boqiang Ma, Sewage sludge disintegration by combined treatment of alkaline+high pressure homogenization, Bioresource Technology, 123, (514), (2012).
- Qiang Li, Jean P. Aucamp, Alison Tang, Alex Chatel and Mike Hoare, Use of focused acoustics for cell disruption to provide ultra scale‐down insights of microbial homogenization and its bioprocess impact—recovery of antibody fragments from rec E. coli, Biotechnology and Bioengineering, 109, 8, (2059-2069), (2012).
- Yuxuan Zhang, Panyue Zhang, Boqiang Ma, Hao Wu, Sheng Zhang and Xin Xu, Sewage sludge disintegration by high-pressure homogenization: A sludge disintegration model, Journal of Environmental Sciences, 24, 5, (814), (2012).
- Magdalena Klimek-Ochab, Małgorzata Brzezińska-Rodak, Ewa Żymańczyk-Duda, Barbara Lejczak and Paweł Kafarski, Comparative study of fungal cell disruption—scope and limitations of the methods, Folia Microbiologica, 56, 5, (469), (2011).
- S.T.L. Harrison, Cell Disruption, Comprehensive Biotechnology, 10.1016/B978-0-08-088504-9.00127-6, (619-640), (2011).
- Duarte Miguel F. Prazeres, Primary Isolation, Plasmid Biopharmaceuticals, (427-453), (2011).
- Huzaifa S. Choonia and S.S. Lele, β-Galactosidase release kinetics during ultrasonic disruption of Lactobacillus acidophilus isolated from fermented Eleusine coracana, Food and Bioproducts Processing, 10.1016/j.fbp.2010.08.009, 89, 4, (288-293), (2011).
- Mehdi Shahini, John Yeow and Morteza Ahmadi, Cell Lysis Techniques in Lab-on-a-Chip Technology, Microfluidics and Nanofluidics Handbook, 10.1201/b11377-24, (927-949), (2012).
- Anurag S. Rathore and Varsha S. Joshi, Scaledown of Biopharmaceutical Purification Operations, Encyclopedia of Industrial Biotechnology, (127-146), (2013).
- F. A. P. Garcia, Cell Wall Disruption and Lysis, Encyclopedia of Industrial Biotechnology, (1-12), (2009).
- Francesco Donsì, Giovanna Ferrari and Paola Maresca, High-Pressure Homogenization for Food Sanitization, Global Issues in Food Science and Technology, 10.1016/B978-0-12-374124-0.00019-3, (309-352), (2009).
- Horst Schütte and Maria‐Regina Kula, Cell Disruption and Isolation of Non‐Secreted Products, Biotechnology Set, (505-526), (2008).
- Anton P. J. Middelberg, The Release of Intracellular Bioproducts, Bioseparation and Bioprocessing, (131-164), (2008).
- Horst Schütte and Maria‐Regina Kula, Cell Disruption and Isolation of Non‐Secreted Products, Biotechnology, (505-526), (2008).
- Xiaodong Ren, Dawei Yu, Lei Yu, Gui Gao, Siping Han and Yan Feng, A new study of cell disruption to release recombinant thermostable enzyme from Escherichia coli by thermolysis, Journal of Biotechnology, 129, 4, (668), (2007).
- Ann M. J. Diels and Chris W. Michiels, High-Pressure Homogenization as a Non-Thermal Technique for the Inactivation of Microorganisms, Critical Reviews in Microbiology, 10.1080/10408410601023516, 32, 4, (201-216), (2008).
- Vivek D. Farkade, Susan T.L. Harrison and Aniruddha B. Pandit, Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis, Biochemical Engineering Journal, 10.1016/j.bej.2006.05.015, 31, 1, (25-30), (2006).
- Kimberly L. Ogden, Bioprocess Engineering, Reviews in Cell Biology and Molecular Medicine, (2006).
- B. Balasundaram and S. T. L. Harrison, Study of Physical and Biological Factors Involved in the Disruption of E. coli by Hydrodynamic Cavitation, Biotechnology Progress, 22, 3, (907-913), (2008).
- Vivek D. Farkade, Sue Harrison and Aniruddha B. Pandit, Heat induced translocation of proteins and enzymes within the cell: an effective way to optimize the microbial cell disruption process, Biochemical Engineering Journal, 10.1016/j.bej.2005.01.001, 23, 3, (247-257), (2005).
- Ann M.J. Diels, Lien Callewaert, Elke Y. Wuytack, Barbara Masschalck and Chris W. Michiels, Inactivation of Escherichia coli by high-pressure homogenisation is influenced by fluid viscosity but not by water activity and product composition, International Journal of Food Microbiology, 10.1016/j.ijfoodmicro.2004.11.011, 101, 3, (281-291), (2005).
- Pim van Hee, Anton P.J. Middelberg, Rob G.J.M. van der Lans and Luuk A.M. van der Wielen, Relation between cell disruption conditions, cell debris particle size, and inclusion body release, Biotechnology and Bioengineering, 88, 1, (100-110), (2004).
- F.A.P. Garcia, Cell Disruption and Lysis, Encyclopedia of Bioprocess Technology, (2002).
- Jana Geciova, Dean Bury and Paul Jelen, Methods for disruption of microbial cells for potential use in the dairy industry—a review, International Dairy Journal, 12, 6, (541), (2002).
- B. Balasundaram and A.B. Pandit, Significance of location of enzymes on their release during microbial cell disruption, Biotechnology and Bioengineering, 75, 5, (607-614), (2001).
- B. Balasundaram and A.B. Pandit, Selective release of invertase by hydrodynamic cavitation, Biochemical Engineering Journal, 10.1016/S1369-703X(01)00114-0, 8, 3, (251-256), (2001).
- R Nandakumar, A.M Gounot and B Mattiasson, Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption, Journal of Biotechnology, 83, 3, (211), (2000).
- Seiichi Morita, Hiroshi Umakoshi and Ryoichi Kuboi, Characterization and on-line monitoring of cell disruption and lysis using dielectric measurement, Journal of Bioscience and Bioengineering, 10.1016/S1389-1723(99)80180-7, 88, 1, (78-84), (1999).
- Ajit Sadana, Steps in Bioseparation Processes, Bioseparation of Proteins - Unfolding/Folding and Validations, 10.1016/S0149-6395(98)80030-5, (19-59), (1998).
- G Larsson, S.B Jørgensen, M.N Pons, B Sonnleitner, A Tijsterman and N Titchener-Hooker, Biochemical engineering science, Journal of Biotechnology, 10.1016/S0168-1656(97)00158-2, 59, 1-2, (3-9), (1997).
- Andrew R. Kleinig and Anton P. J. Middelberg, Numerical and experimental study of a homogenizer impinging jet, AIChE Journal, 43, 4, (1100-1107), (2004).
- Hiroshi Umakoshi, Koji Yano, Ryoichi Kuboi and Isao Komasawa, Extractive Cultivation of Recombinant Escherichia coli using Aqueous Two‐Phase Systems for Production and Separation of Intracellular Heat Shock Proteins, Biotechnology Progress, 12, 1, (51-56), (2008).
- Ryoichi Kuboi, Hiroshi Umakoshi, Naoyuki Takagi and Isao Komasawa, Optimal disruption methods for the selective recovery of β-galactosidase from Escherichia coli, Journal of Fermentation and Bioengineering, 79, 4, (335), (1995).
- Bernard R. Glick, Metabolic load and heterologous gene expression, Biotechnology Advances, 13, 2, (247), (1995).
- Alfred Carlson, Mark Signs, Laura Liermann, Robert Boor and K. Jim Jem, Mechanical disruption of Escherichia coli for plasmid recovery, Biotechnology and Bioengineering, 48, 4, (303-315), (2004).
- Stuart M. Bailey, Paul H. Blum and Michael M. Meagher, Improved Homogenization of Recombinant Escherichia coli following Pretreatment with Guanidine Hydrochloride, Biotechnology Progress, 11, 5, (533-539), (2008).
- Anton P.J. Middelberg, Process-scale disruption of microorganisms, Biotechnology Advances, 13, 3, (491), (1995).
- Clark V. Baldwin and Campbell W. Robinson, Enhanced disruption of Candida utilis using enzymatic pretreatment and high‐pressure homogenization, Biotechnology and Bioengineering, 43, 1, (46-56), (2004).
- P. T. Milburn and P. Dunnill, The release of virus‐like particles from recombinant Saccharomyces cerevisiae: Effect of freezing and thawing on homogenization and bead milling, Biotechnology and Bioengineering, 44, 6, (736-744), (2004).
- C. Baldwin and C. W. Robinson, Increased disruption resistance of Candida utilis grown from survivors of enzymatic lysis combined with high-pressure homogenization, Letters in Applied Microbiology, 15, 2, (59), (1992).
- Gerda Grund, Campbell W. Robinson and Bernard R. Glick, Protein type effects on steady-state crossflow membrane ultrafiltration fluxes and protein transmission, Journal of Membrane Science, 70, 2-3, (177), (1992).
- Anton P. J. Middelberg, Brian K. O'Neill, I. David L. Bogle and Mark A. Snoswell, A novel technique for the measurement of disruption in high‐pressure homogenization: Studies on E. coli containing recombinant inclusion bodies, Biotechnology and Bioengineering, 38, 4, (363-370), (2004).
- J.-C. Vuillemard, Recent advances in the large-scale production of lipid vesicles for use in food products: microfluidization, Journal of Microencapsulation, 8, 4, (547), (1991).
- Iréne Agerkvist and Sven‐Olof Enfors, Characterization of E. coli cell disintegrates from a bead mill and high pressure homogenizers, Biotechnology and Bioengineering, 36, 11, (1083-1089), (2004).
- R. DIEKMANN and D. C. HEMPEL, Immobilization Techniques, Bioreactors, and Improvements in Downstream Processing, Annals of the New York Academy of Sciences, 613, 1, (255-264), (2006).
