Primary Isolation Techniques and Integrated Process
Mechanical disruption of Escherichia coli for plasmid recovery
Abstract
Five different mechanical cell disruption processes were evaluated as methods to extract plasmids from bacterial cells. The methods used were sonication, nebulization homogenization, microfluidization, and bead milling. The recovery yields of intact plasmids from the various methods were measured by quantitative gel electrophoresis. Bead milling and microfluidization were found to have the highest potential for large scale extraction with total intact recoveries of over 90% and around 50%, respectively. Other methods resulted in substantial plasmid degradation, with recoveries no greater than 20% of the total intact plasmid. © 1995 John Wiley & Sons, Inc.
Citing Literature
Number of times cited: 46
- Cornelia Walther and Astrid Dürauer, Microscale disruption of microorganisms for parallelized process development, Biotechnology Journal, 12, 7, (2017).
- Keith R Paton, James Anderson, Andrew J Pollard and Toby Sainsbury, Production of few-layer graphene by microfluidization, Materials Research Express, 4, 2, (025604), (2017).
- Magdalena Plotka, Mateusz Wozniak, Tadeusz Kaczorowski and Hideyuki Doi, Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR, PLOS ONE, 12, 1, (e0169846), (2017).
- Simon Bystryak, Rasa Santockyte and Alexey S. Peshkovsky, Cell disruption of S. cerevisiae by scalable high-intensity ultrasound, Biochemical Engineering Journal, 10.1016/j.bej.2015.03.014, 99, (99-106), (2015).
- Alan G. Mathew, Real‐Time PCR, Handbook of Food Safety Engineering, (217-257), (2012).
- José C. Nunes, António M. Morão, Catherine Nunes, Maria Teresa Pessoa de Amorim, Isabel C. Escobar and João A. Queiroz, Plasmid DNA recovery from fermentation broths by a combined process of micro- and ultrafiltration: Modeling and application, Journal of Membrane Science, 415-416, (24), (2012).
- Federica Ciamponi, Craig Duckham and Nicola Tirelli, Yeast cells as microcapsules. Analytical tools and process variables in the encapsulation of hydrophobes in S. cerevisiae, Applied Microbiology and Biotechnology, 95, 6, (1445), (2012).
- Duarte Miguel F. Prazeres, Concluding Remarks and Outlook, Plasmid Biopharmaceuticals, (563-578), (2011).
- Duarte Miguel F. Prazeres, Primary Isolation, Plasmid Biopharmaceuticals, (427-453), (2011).
- Hari Kamaraju, Kenneth Wetzel and William J. Kelly, Modeling shear‐induced CHO cell damage in a rotary positive displacement pump, Biotechnology Progress, 26, 6, (1606-1615), (2010).
- Naomichi Yamamoto, Minoru Kimura, Hideaki Matsuki and Yukio Yanagisawa, Optimization of a real-time PCR assay to quantitate airborne fungi collected on a gelatin filter, Journal of Bioscience and Bioengineering, 109, 1, (83), (2010).
- Yusuf Chisti, Shear Sensitivity, Encyclopedia of Industrial Biotechnology, (1-40), (2009).
- F. A. P. Garcia, Cell Wall Disruption and Lysis, Encyclopedia of Industrial Biotechnology, (1-12), (2009).
- Naomichi Yamamoto, Yasunari Matsuzaka, Minoru Kimura, Hideaki Matsuki and Yukio Yanagisawa, Comparison of dry- and wet-based fine bead homogenizations to extract DNA from fungal spores, Journal of Bioscience and Bioengineering, 107, 4, (464), (2009).
- Kirsten Tschapalda, Nadine Streitner, Carsten Voß and Erwin Flaschel, Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction, Applied Microbiology and Biotechnology, 84, 1, (199), (2009).
- Frank Luechau, Tau Chuan Ling and Andrew Lyddiatt, Primary capture of high molecular weight nucleic acids using aqueous two-phase systems, Separation and Purification Technology, 66, 1, (202), (2009).
- F.J. Meacle, H. Zhang, I. Papantoniou, J.M. Ward, N.J. Titchener‐Hooker and M. Hoare, Degradation of supercoiled plasmid DNA within a capillary device, Biotechnology and Bioengineering, 97, 5, (1148-1157), (2006).
- Hu Zhang, Simyee Kong, Andrew Booth, Rihab Boushaba, M. Susana Levy and Michael Hoare, Prediction of Shear Damage of Plasmid DNA in Pump and Centrifuge Operations Using an Ultra Scale‐Down Device, Biotechnology Progress, 23, 4, (858-865), (2008).
- Guilherme N. M. Ferreira, Duarte M. F. Prazeres, Joaquim M. S. Cabral and Martin Schleef, Plasmid Manufacturing – An Overview, Plasmids for Therapy and Vaccination, (193-236), (2007).
- Jochen Urthaler, Christine Ascher, Helga Wöhrer and Roman Necina, Automated alkaline lysis for industrial scale cGMP production of pharmaceutical grade plasmid-DNA, Journal of Biotechnology, 128, 1, (132), (2007).
- Hu Zhang, Simyee Kong, Andrew Booth, Rihab Boushaba, M. Susana Levy and Michael Hoare, Prediction of Shear Damage of Plasmid DNA in Pump and Centrifuge Operations Using an Ultra Scale-Down Device, Biotechnology Progress, 23, 4, (858), (2007).
- Jason C. Murphy, Tony Cano, George E. Fox and Richard C. Willson, Compaction Agent Protection of Nucleic Acids during Mechanical Lysis, Biotechnology Progress, 22, 2, (519-522), (2008).
- G. Chan, A.J. Booth, K. Mannweiler and M. Hoare, Ultra scale‐down studies of the effect of flow and impact conditions during E. coli cell processing, Biotechnology and Bioengineering, 95, 4, (671-683), (2006).
- Juan G. Beltrán, Richard L. Leask and Wayne A. Brown, Activity and stability of caffeine demethylases found in Pseudomonas putida IF-3, Biochemical Engineering Journal, 31, 1, (8), (2006).
- Vivek D. Farkade, Sue Harrison and Aniruddha B. Pandit, Heat induced translocation of proteins and enzymes within the cell: an effective way to optimize the microbial cell disruption process, Biochemical Engineering Journal, 10.1016/j.bej.2005.01.001, 23, 3, (247-257), (2005).
- D.M.F. Prazeres and G.N.M. Ferreira, Design of flowsheets for the recovery and purification of plasmids for gene therapy and DNA vaccination, Chemical Engineering and Processing: Process Intensification, 10.1016/j.cep.2003.02.002, 43, 5, (609-624), (2004).
- William J. Kelly and Kenneth R. Muske, Optimal operation of high-pressure homogenization for intracellular product recovery, Bioprocess and Biosystems Engineering, 27, 1, (25), (2004).
- Zhijun Wang, Zhenghong Yuan and Ulrich R. Hengge, Processing of plasmid DNA with ColE1-like replication origin, Plasmid, 51, 3, (149), (2004).
- Michael Clemson and William J. Kelly, Optimizing alkaline lysis for DNA plasmid recovery, Biotechnology and Applied Biochemistry, 37, 3, (235-244), (2010).
- William J. Kelly, MINIREVIEW: Perspectives on plasmid‐based gene therapy: challenges for the product and the process, Biotechnology and Applied Biochemistry, 37, 3, (219-223), (2010).
- Kristala Jones Prather, Sangeetha Sagar, Jason Murphy and Michel Chartrain, Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification, Enzyme and Microbial Technology, 10.1016/S0141-0229(03)00205-9, 33, 7, (865-883), (2003).
- F.A.P. Garcia, Cell Disruption and Lysis, Encyclopedia of Bioprocess Technology, (2002).
- J. Miller, M. Rogowski and W. Kelly, Using a CFD Model To Understand the Fluid Dynamics Promoting E. coli Breakage in a High‐Pressure Homogenizer, Biotechnology Progress, 18, 5, (1060-1067), (2008).
- Zhijun Wang, Guowei Le, Yonghui Shi and Grzegorz Wegrzyn, Studies on recovery plasmid DNA from Echerichia coli by heat treatment, Process Biochemistry, 38, 2, (199), (2002). Proceedings of 2002 American Control Conference Anchorage, AK, USA Proceedings of the 2002 American Control Conference (IEEE Cat. No.CH37301) IEEE , (2002). 0-7803-7298-0 W.J. Kelly, J.C. Miller, M.A. Rogowski and K.R. Muske Model-based control of a high pressure homogenizer , (2002). 2891 2896 vol.4 1025228 , 10.1109/ACC.2002.1025228 http://ieeexplore.ieee.org/document/1025228/
- B. Balasundaram and A.B. Pandit, Selective release of invertase by hydrodynamic cavitation, Biochemical Engineering Journal, 10.1016/S1369-703X(01)00114-0, 8, 3, (251-256), (2001).
- S. Chamsart, H. Patel, J. A. J. Hanak, A. G. Hitchcock and A. W. Nienow, The impact of fluid‐dynamic‐generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products, Biotechnology and Bioengineering, 75, 4, (387-392), (2001).
- D.M.F Prazeres, G.A Monteiro, G.N.M Ferreira, M.M Diogo, S.C Ribeiro and J.M.S Cabral, Purification of plasmids for gene therapy and DNA vaccination, , 10.1016/S1387-2656(01)07031-4, (1-30), (2001).
- B. Balasundaram and A.B. Pandit, Significance of location of enzymes on their release during microbial cell disruption, Biotechnology and Bioengineering, 75, 5, (607-614), (2001).
- Guilherme N.M. Ferreira, Gabriel A. Monteiro, Duarte M.F. Prazeres and Joaquim M.S. Cabral, Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications, Trends in Biotechnology, 10.1016/S0167-7799(00)01475-X, 18, 9, (380-388), (2000).
- M.Susana Levy, Ronan D O’Kennedy, Parviz Ayazi-Shamlou and Peter Dunnill, Biochemical engineering approaches to the challenges of producing pure plasmid DNA, Trends in Biotechnology, 18, 7, (296), (2000).
- G. A. Monteiro, G. N. M. Ferreira, J. M. S. Cabral and D. M. F. Prazeres, Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy, Biotechnology and Bioengineering, 66, 3, (189-194), (2000).
- Duarte M.F. Prazeres, Guilherme N.M. Ferreira, Gabriel A. Monteiro, Charles L. Cooney and Joaquim M.S. Cabral, Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks, Trends in Biotechnology, 17, 4, (169), (1999).
- Andrew Lyddiatt and Deirdre A O'Sullivan, Biochemical recovery and purification of gene therapy vectors, Current Opinion in Biotechnology, 9, 2, (177), (1998).
- G Larsson, S.B Jørgensen, M.N Pons, B Sonnleitner, A Tijsterman and N Titchener-Hooker, Biochemical engineering science, Journal of Biotechnology, 10.1016/S0168-1656(97)00158-2, 59, 1-2, (3-9), (1997).
- Adriana Padilla-Zamudio, J. Lucero-Acuña, Patricia Guerrero-Germán, Jaime Ortega-López and Armando Tejeda-Mansir, Efficient Disruption of Escherichia coli for Plasmid DNA Recovery in a Bead Mill, Applied Sciences, 10.3390/app8010030, 8, 1, (30), (2017).
