Organische Chemie
Synthese von Oligodesoxynucleotiden über Phosphorsäuretriester
Abstract
Eine neue Methode zur Synthese von Oligodesoxynucleotiden wird untersucht, bei der die Internucleotid‐Phosphorsäuregruppe mit dem durch Zink abspaltbaren Trichloräthylrest verestert ist. Auf diese Weise werden d(TpT, d(TpTpT), d(TpTpTpT), d(CpT), d(ApT), d(ApA) und d(ApApA) synthetisiert. Mit Hilfe der kernmagnetischen Resonanz wird wahrscheinlich gemacht, daß durch Aktivierung von Nucleosid‐3′‐phosphorsäure‐trichloräthylestern mit 2.4.6‐Triisopropyl‐benzolsulfonylchlorid ein Pyrophosphorsäure‐tetraester (18) gebildet wird, der mit der 5′‐OH‐Gruppe eines Nucleosids zu dem Dinucleosidphosphorsäure‐trichloräthylester reagiert.
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