Fast Track
Free Access
A high‐affinity human monoclonal antibody specific to the alternatively spliced EDA domain of fibronectin efficiently targets tumor neo‐vasculature in vivo
Abstract
The alternatively spliced extra‐domain B of fibronectin is one of the best characterized markers of tumor angiogenesis. Similarly, the extra‐domain A (EDA), which can also be inserted in the fibronectin transcript by a mechanism of alternative splicing, has been shown to preferentially accumulate around new blood vessels in certain tumors, but this antigen has not been investigated so far as a target for antibody‐based biomolecular intervention. We here describe the generation of 3 human monoclonal antibodies (named F8, B7 and D5), which recognize the same epitope of EDA, but which differ in terms of their dissociation constant to the human antigen (KD = 3.1, 16 and 17 nM, measured for monomeric preparations of the F8, B7 and D5 antibodies, respectively, in recombinant scFv format). When the 3 antibody fragments were cloned and expressed with a 5 amino acid linker, the 3 resulting homodimeric antibody preparations displayed comparable tumor: organ ratios in quantitative biodistribution studies, performed in immunocompetent 129SvEv mice, bearing subcutaneous syngeneic F9 murine tumors. The percent injected dose per gram (%ID/g) values in tumors 24 hr after intravenous injection were 9.3, 10.2 and 13 for F8, B7 and D5, respectively. The F8 antibody may serve as useful building block for the development of antibody‐based targeted anti‐cancer therapeutics. Preclinical and clinical investigations are facilitated by the fact that F8 recognizes the human and mouse antigen with comparable affinity, and by the observation that EDA over‐expression is detectable not only in solid tumors, but also in hematological malignancies. © 2008 Wiley‐Liss, Inc.
Citing Literature
Number of times cited: 114
- Kittima Amornwachirabodee, Nattapol Tantimekin, Porntip Pan-In, Tanapat Palaga, Prompong Pienpinijtham, Chonlatip Pipattanaboon, Thanyada Sukmanee, Patcharee Ritprajak, Promchat Charoenpat, Pannamthip Pitaksajjakul, Pongrama Ramasoota and Supason Wanichwecharungruang, Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane, Scientific Reports, 8, 1, (2018).
- Barbara Ziffels, Francesca Pretto and Dario Neri, Intratumoral administration of IL2- and TNF-based fusion proteins cures cancer without establishing protective immunity, Immunotherapy, 10, 3, (177), (2018).
- Aleksandra Borek, Aleksandra Sokolowska-Wedzina, Grzegorz Chodaczek, Jacek Otlewski and Mark Isalan, Generation of high-affinity, internalizing anti-FGFR2 single-chain variable antibody fragment fused with Fc for targeting gastrointestinal cancers, PLOS ONE, 13, 2, (e0192194), (2018).
- Christophe Dumet, Jérémy Pottier, Valérie Gouilleux and Hervé Watier, New structural formats of therapeutic antibodies for rheumatology, Joint Bone Spine, 85, 1, (47), (2018).
- Stine F. Pedersen, Ivana Novak, Frauke Alves, Albrecht Schwab and Luis A. Pardo, Alternating pH landscapes shape epithelial cancer initiation and progression: Focus on pancreatic cancer, BioEssays, 39, 6, (2017).
- Zheng Han and Zheng-Rong Lu, Targeting fibronectin for cancer imaging and therapy, J. Mater. Chem. B, 10.1039/C6TB02008A, 5, 4, (639-654), (2017).
- Jesper Just, Simon Lykkemark, Charlotte H. Nielsen, Ali R. Roshenas, Kim R. Drasbek, Steen V. Petersen, Toke Bek and Peter Kristensen, Pericyte modulation by a functional antibody obtained by a novel single‐cell selection strategy, Microcirculation, 24, 6, (2017).
- Agata Radwanska, Dominique Grall, Sébastien Schaub, Stéphanie Beghelli-de la Forest Divonne, Delphine Ciais, Samah Rekima, Tristan Rupp, Anne Sudaka, Gertraud Orend and Ellen Van Obberghen-Schilling, Counterbalancing anti-adhesive effects of Tenascin-C through fibronectin expression in endothelial cells, Scientific Reports, 7, 1, (2017).
- Christophe Dumet, Jérémy Pottier, Valérie Gouilleux and Hervé Watier, Les nouveaux formats structuraux des anticorps thérapeutiques en rhumatologie, Revue du Rhumatisme, 84, 5, (385), (2017).
- Jonathan D. Kiefer and Dario Neri, Immunocytokines and bispecific antibodies: two complementary strategies for the selective activation of immune cells at the tumor site, Immunological Reviews, 270, 1, (178-192), (2016).
- G. Acker, A. Palumbo, D. Neri, P. Vajkoczy and M. Czabanka, F8-SIP mediated targeted photodynamic therapy leads to microvascular dysfunction and reduced glioma growth, Journal of Neuro-Oncology, 10.1007/s11060-016-2143-8, 129, 1, (33-38), (2016).
- Franziska Bootz and Dario Neri, Immunocytokines: a novel class of products for the treatment of chronic inflammation and autoimmune conditions, Drug Discovery Today, 10.1016/j.drudis.2015.10.012, 21, 1, (180-189), (2016).
- Moncef Zouali, Antibodies, eLS, (1-13), (2016).
- F. Bootz, D. Venetz, B. Ziffels and D. Neri, Different tissue distribution properties for glycosylation variants of fusion proteins containing the p40 subunit of murine interleukin-12, Protein Engineering Design and Selection, 10.1093/protein/gzw038, 29, 10, (445-455), (2016).
- Joanna Z. Kawalkowska, Teresa Hemmerle, Francesca Pretto, Mattia Matasci, Dario Neri and Richard O. Williams, Targeted IL‐4 therapy synergizes with dexamethasone to induce a state of tolerance by promoting Treg cells and macrophages in mice with arthritis, European Journal of Immunology, 46, 5, (1246-1257), (2016).
- Barbara Ziffels, Johanna Ospel, Katja Grün, Dario Neri, Alexander Pfeil, Michael Fritzenwanger, Hans R. Figulla, Christian Jung, Alexander Berndt and Marcus Franz, Detection of Soluble ED-A+Fibronectin and Evaluation as Novel Serum Biomarker for Cardiac Tissue Remodeling, Disease Markers, 2016, (1), (2016).
- Dario Venetz, Danil Koovely, Bruce Weder and Dario Neri, Targeted Reconstitution of Cytokine Activity upon Antigen Binding using Split Cytokine Antibody Fusion Proteins, Journal of Biological Chemistry, 10.1074/jbc.M116.737734, 291, 35, (18139-18147), (2016).
- Asif Amin, Naveed Anjum Chikan, Taseem A. Mokhdomi, Shoiab Bukhari, Aabid M. Koul, Basit Amin Shah, Fatemeh Rahimi Gharemirshamlu, Asrar H. Wafai, Ayub Qadri and Raies A. Qadri, Irigenin, a novel lead from Western Himalayan chemiome inhibits Fibronectin-Extra Domain A induced metastasis in Lung cancer cells, Scientific Reports, 6, 1, (2016).
- Mathieu Ferrari, Shimobi C. Onuoha and Costantino Pitzalis, Going with the flow: harnessing the power of the vasculature for targeted therapy in rheumatoid arthritis, Drug Discovery Today, 10.1016/j.drudis.2015.10.014, 21, 1, (172-179), (2016).
- Franziska Bootz, Barbara Ziffels and Dario Neri, Antibody-Based Targeted Delivery of Interleukin-22 Promotes Rapid Clinical Recovery in Mice With DSS-Induced Colitis, Inflammatory Bowel Diseases, 22, 9, (2098), (2016).
- Sabrina Kraft, Verena Klemis, Carla Sens, Thorsten Lenhard, Christian Jacobi, Yvonne Samstag, Guido Wabnitz, Michael Kirschfink, Reinhard Wallich, G. Maria Hänsch and Inaam A. Nakchbandi, Identification and characterization of a unique role for EDB fibronectin in phagocytosis, Journal of Molecular Medicine, 94, 5, (567), (2016).
- Heena Kumra and Dieter P. Reinhardt, Fibronectin-targeted drug delivery in cancer, Advanced Drug Delivery Reviews, 10.1016/j.addr.2015.11.014, 97, (101-110), (2016).
- Lu Zhang, Xiaoqiao Liu, Xuegong Zhang and Ronghua Chen, Identification of important long non-coding RNAs and highly recurrent aberrant alternative splicing events in hepatocellular carcinoma through integrative analysis of multiple RNA-Seq datasets, Molecular Genetics and Genomics, 291, 3, (1035), (2016).
- Franziska Bootz, Anja Sophie Schmid and Dario Neri, Alternatively Spliced EDA Domain of Fibronectin Is a Target for Pharmacodelivery Applications in Inflammatory Bowel Disease, Inflammatory Bowel Diseases, 21, 8, (1908), (2015).
- Edyta Petters, Aleksandra Sokolowska-Wedzina and Jacek Otlewski, Selection and Characterization of Single Chain Antibody Fragments Specific for Hsp90 as a Potential Cancer Targeting Molecule, International Journal of Molecular Sciences, 16, 12, (19920), (2015).
- Christian Hess and Dario Neri, The antibody-mediated targeted delivery of interleukin-13 to syngeneic murine tumors mediates a potent anticancer activity, Cancer Immunology, Immunotherapy, 64, 5, (635), (2015).
- Florent Samain and Giulio Casi, Small targeted cytotoxics from DNA-encoded chemical libraries, Current Opinion in Chemical Biology, 10.1016/j.cbpa.2015.02.009, 26, (72-79), (2015).
- Emil Bujak, Francesca Pretto and Dario Neri, Generation and tumor recognition properties of two human monoclonal antibodies specific to cell surface anionic phospholipids, Investigational New Drugs, 33, 4, (791), (2015).
- Sébastien Wieckowski, Teresa Hemmerle, Spasenja Savic Prince, Béatrice Dolder Schlienger, Sven Hillinger, Dario Neri and Alfred Zippelius, Therapeutic efficacy of the F8-IL2 immunocytokine in a metastatic mouse model of lung adenocarcinoma, Lung Cancer, 88, 1, (9), (2015).
- Giulio Casi and Dario Neri, Antibody–Drug Conjugates and Small Molecule–Drug Conjugates: Opportunities and Challenges for the Development of Selective Anticancer Cytotoxic Agents, Journal of Medicinal Chemistry, 58, 22, (8751), (2015).
- Giulio Casi and Dario Neri, Noninternalizing Targeted Cytotoxics for Cancer Therapy, Molecular Pharmaceutics, 12, 6, (1880), (2015).
- Federica Quattrone, Ana Maria Sanchez, Maria Pannese, Teresa Hemmerle, Paola Viganò, Massimo Candiani, Felice Petraglia, Dario Neri and Paola Panina-Bordignon, The Targeted Delivery of Interleukin 4 Inhibits Development of Endometriotic Lesions in a Mouse Model, Reproductive Sciences, 22, 9, (1143), (2015).
- Assaf Ganoth, Keren Cohen Merimi and Dan Peer, Overcoming multidrug resistance with nanomedicines, Expert Opinion on Drug Delivery, 12, 2, (223), (2015).
- Marcus Franz, Fabia Doll, Katja Grün, Petra Richter, Nilay Köse, Barbara Ziffels, Harald Schubert, Hans R. Figulla, Christian Jung, Jan Gummert, André Renner, Dario Neri and Alexander Berndt, Targeted delivery of interleukin-10 to chronic cardiac allograft rejection using a human antibody specific to the extra domain A of fibronectin, International Journal of Cardiology, 195, (311), (2015).
- Fortunato Ferrara, Sara D’Angelo, Tiziano Gaiotto, Leslie Naranjo, Hongzhao Tian, Susanne Gräslund, Elena Dobrovetsky, Peter Hraber, Fridtjof Lund-Johansen, Silvia Saragozza, Daniele Sblattero, Csaba Kiss and Andrew RM Bradbury, Recombinant renewable polyclonal antibodies, mAbs, 7, 1, (32), (2015).
- Emil Bujak, Danilo Ritz and Dario Neri, A Monoclonal Antibody to Human DLK1 Reveals Differential Expression in Cancer and Absence in Healthy Tissues, Antibodies, 4, 4, (71), (2015).
- Dario Venetz, Christian Hess, Chia-wei Lin, Markus Aebi and Dario Neri, Glycosylation profiles determine extravasation and disease-targeting properties of armed antibodies, Proceedings of the National Academy of Sciences, 112, 7, (2000), (2015).
- Ziad Julier, Mikaël M. Martino, Alexandre de Titta, Laura Jeanbart and Jeffrey A. Hubbell, The TLR4 Agonist Fibronectin Extra Domain A is Cryptic, Exposed by Elastase-2; use in a fibrin matrix cancer vaccine, Scientific Reports, 10.1038/srep08569, 5, 1, (2015).
- Teresa Hemmerle and Dario Neri, The antibody‐based targeted delivery of interleukin‐4 and 12 to the tumor neovasculature eradicates tumors in three mouse models of cancer, International Journal of Cancer, 134, 2, (467-477), (2013).
- Verena Strassberger, Katrin L. Gutbrodt, Nikolaus Krall, Christoph Roesli, Hitoshi Takizawa, Markus G. Manz, Tim Fugmann and Dario Neri, A comprehensive surface proteome analysis of myeloid leukemia cell lines for therapeutic antibody development, Journal of Proteomics, 99, (138), (2014).
- Emil Bujak, Francesca Pretto, Danilo Ritz, Laura Gualandi, Sarah Wulhfard and Dario Neri, Monoclonal antibodies to murine thrombospondin-1 and thrombospondin-2 reveal differential expression patterns in cancer and low antigen expression in normal tissues, Experimental Cell Research, 327, 1, (135), (2014).
- Christian Hess and Dario Neri, Tumor-targeting properties of novel immunocytokines based on murine IL1β and IL6, Protein Engineering, Design and Selection, 27, 6, (207), (2014).
- Chai-Hong Yeong, Mu-hua Cheng and Kwan-Hoong Ng, Therapeutic radionuclides in nuclear medicine: current and future prospects, Journal of Zhejiang University SCIENCE B, 15, 10, (845), (2014).
- Francesca Pretto, Giuliano Elia, Nadia Castioni and Dario Neri, Preclinical evaluation of IL2-based immunocytokines supports their use in combination with dacarbazine, paclitaxel and TNF-based immunotherapy, Cancer Immunology, Immunotherapy, 63, 9, (901), (2014).
- Teresa Hemmerle, Silvana Zgraggen, Mattia Matasci, Cornelia Halin, Michael Detmar and Dario Neri, Antibody-mediated delivery of interleukin 4 to the neo-vasculature reduces chronic skin inflammation, Journal of Dermatological Science, 10.1016/j.jdermsci.2014.07.012, 76, 2, (96-103), (2014).
- Christian Hess and Dario Neri, Evaluation of antibody–chemokine fusion proteins for tumor-targeting applications, Experimental Biology and Medicine, 10.1177/1535370214536667, 239, 7, (842-852), (2014).
- Marcel Weber, Emil Bujak, Alessia Putelli, Alessandra Villa, Mattia Matasci, Laura Gualandi, Teresa Hemmerle, Sarah Wulhfard, Dario Neri and Mark Isalan, A Highly Functional Synthetic Phage Display Library Containing over 40 Billion Human Antibody Clones, PLoS ONE, 9, 6, (e100000), (2014).
- Fiona Mack, Michael Ritchie and Puja Sapra, The Next Generation of Antibody Drug Conjugates, Seminars in Oncology, 41, 5, (637), (2014).
- Alessia Putelli, Jonathan D. Kiefer, Matthias Zadory, Mattia Matasci and Dario Neri, A Fibrin-Specific Monoclonal Antibody from a Designed Phage Display Library Inhibits Clot Formation and Localizes to Tumors In Vivo, Journal of Molecular Biology, 426, 21, (3606), (2014).
- T. Hemmerle, F. Doll and D. Neri, Antibody-based delivery of IL4 to the neovasculature cures mice with arthritis, Proceedings of the National Academy of Sciences, 10.1073/pnas.1402783111, 111, 33, (12008-12012), (2014).
- Lisa Mullen, Gill Adams, Lorna Layward, Sandrine Vessillier, Alex Annenkov, Gayatri Mittal, Anne Rigby, Michelle Sclanders, David Baker, David Gould and Yuti Chernajovsky, Latent cytokines for targeted therapy of inflammatory disorders, Expert Opinion on Drug Delivery, 10.1517/17425247.2014.863872, 11, 1, (101-110), (2013).
- Teresa Hemmerle, Christian Hess, Dario Venetz and Dario Neri, Tumor targeting properties of antibody fusion proteins based on different members of the murine tumor necrosis superfamily, Journal of Biotechnology, 10.1016/j.jbiotec.2013.12.010, 172, (73-76), (2014).
- Niccolò Ravenni, Marcel Weber and Dario Neri, A human monoclonal antibody specific to placental alkaline phosphatase, a marker of ovarian cancer, mAbs, 6, 1, (86), (2014).
- Giuliano Elia, Tim Fugmann and Dario Neri, From target discovery to clinical trials with armed antibody products, Journal of Proteomics, 107, (50), (2014).
- Anna Johansson, Juliana Hamzah and Ruth Ganss, License for destruction: Tumor-specific cytokine targeting, Trends in Molecular Medicine, 10.1016/j.molmed.2013.10.002, 20, 1, (16-24), (2014).
- Alain Beck, Paul J. Carter, Hans-Peter Gerber, Alexey A. Lugovskoy, Thierry Wurch, Jagath R. Junutula, Roland E Kontermann and Robert Mabry, 8thAnnual European Antibody Congress 2012, mAbs, 5, 3, (339), (2013).
- Fabia Doll, Kathrin Schwager, Teresa Hemmerle and Dario Neri, Murine analogues of etanercept and of F8-IL10 inhibit the progression of collagen-induced arthritis in the mouse, Arthritis Research & Therapy, 10.1186/ar4319, 15, 5, (R138), (2013).
- Martina Steiner, Isabelle Hartmann, Elena Perrino, Giulio Casi, Samatanga Brighton, Ilian Jelesarov, Gonçalo J. L. Bernardes and Dario Neri, Spacer length shapes drug release and therapeutic efficacy of traceless disulfide-linked ADCs targeting the tumor neovasculature, Chem. Sci., 4, 1, (297), (2013).
- Anja von Au, Matthaeus Vasel, Sabrina Kraft, Carla Sens, Norman Hackl, Alexander Marx, Philipp Stroebel, Jörg Hennenlotter, Tilman Todenhöfer, Arnulf Stenzl, Sarah Schott, Hans-Peter Sinn, Antoinette Wetterwald, Justo Lorenzo Bermejo, Marco G Cecchini and Inaam A Nakchbandi, Circulating Fibronectin Controls Tumor Growth, Neoplasia, 15, 8, (925), (2013).
- Martina Steiner, Katrin Gutbrodt, Nikolaus Krall and Dario Neri, Tumor-Targeting Antibody–Anticalin Fusion Proteins for in Vivo Pretargeting Applications, Bioconjugate Chemistry, 24, 2, (234), (2013).
- T Hemmerle, P Probst, L Giovannoni, A J Green, T Meyer and D Neri, The antibody-based targeted delivery of TNF in combination with doxorubicin eradicates sarcomas in mice and confers protective immunity, British Journal of Cancer, 109, 5, (1206), (2013).
- K. L. Gutbrodt, C. Schliemann, L. Giovannoni, K. Frey, T. Pabst, W. Klapper, W. E. Berdel and D. Neri, Antibody-Based Delivery of Interleukin-2 to Neovasculature Has Potent Activity Against Acute Myeloid Leukemia, Science Translational Medicine, 5, 201, (201ra118), (2013).
- Marcus Franz, Ingrid Hilger, Katja Grün, Susanne Kossatz, Petra Richter, Iver Petersen, Christian Jung, Jan Gummert, Hans R. Figulla, Hartwig Kosmehl, Dario Neri, Alexander Berndt and André Renner, Selective imaging of chronic cardiac rejection using a human antibody specific to the alternatively spliced EDA domain of fibronectin, The Journal of Heart and Lung Transplantation, 32, 6, (641), (2013).
- Gonçalo J L Bernardes, Martina Steiner, Isabelle Hartmann, Dario Neri and Giulio Casi, Site-specific chemical modification of antibody fragments using traceless cleavable linkers, Nature Protocols, 8, 11, (2079), (2013).
- Marcus Franz, Alexander Berndt, Dario Neri, Kerstin Galler, Katja Grün, Christiane Porrmann, Franziska Reinbothe, Gerhard Mall, Peter Schlattmann, André Renner, Hans Reiner Figulla, Christian Jung and Friedhelm Küthe, Matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, B+ tenascin-C and ED-A+ fibronectin in dilated cardiomyopathy: Potential impact on disease progression and patients' prognosis, International Journal of Cardiology, 168, 6, (5344), (2013).
- Marcus Franz, Dario Neri and Alexander Berndt, Chronic cardiac allograft rejection: critical role of ED‐A+ fibronectin and implications for targeted therapy strategies, The Journal of Pathology, 226, 4, (557-561), (2012).
- Gonçalo J. L. Bernardes, Giulio Casi, Sabrina Trüssel, Isabelle Hartmann, Kathrin Schwager, Jörg Scheuermann and Dario Neri, A Traceless Vascular‐Targeting Antibody–Drug Conjugate for Cancer Therapy, Angewandte Chemie International Edition, 51, 4, (941-944), (2011).
- Gonçalo J. L. Bernardes, Giulio Casi, Sabrina Trüssel, Isabelle Hartmann, Kathrin Schwager, Jörg Scheuermann and Dario Neri, A Traceless Vascular‐Targeting Antibody–Drug Conjugate for Cancer Therapy, Angewandte Chemie, 124, 4, (965-968), (2011).
- S. Miersch and S.S. Sidhu, Synthetic antibodies: Concepts, potential and practical considerations, Methods, 57, 4, (486), (2012).
- Wesley R. Legant, Christopher S. Chen and Viola Vogel, Force-induced fibronectin assembly and matrix remodeling in a 3D microtissue model of tissue morphogenesis, Integrative Biology, 4, 10, (1164), (2012).
- Sabrina Trüssel, Joerg Scheuermann and Dario Neri, Half‐Life Extension by Binding to Albumin through Small Molecules, Therapeutic Proteins, (285-296), (2012).
- Justyna Bazan, Ireneusz Całkosiński and Andrzej Gamian, Phage display—A powerful technique for immunotherapy, Human Vaccines & Immunotherapeutics, 10.4161/hv.21703, 8, 12, (1817-1828), (2014).
- Nadine Pasche, Katharina Frey and Dario Neri, The targeted delivery of IL17 to the mouse tumor neo-vasculature enhances angiogenesis but does not reduce tumor growth rate, Angiogenesis, 15, 1, (165), (2012).
- Kerstin Galler, Kerstin Junker, Marcus Franz, Julia Hentschel, Petra Richter, Mieczyslaw Gajda, Angela Göhlert, Ferdinand Eggeling, Regine Heller, Raffaella Giavazzi, Dario Neri, Hartwig Kosmehl, Heiko Wunderlich and Alexander Berndt, Differential vascular expression and regulation of oncofetal tenascin-C and fibronectin variants in renal cell carcinoma (RCC): implications for an individualized angiogenesis-related targeted drug delivery, Histochemistry and Cell Biology, 137, 2, (195), (2012).
- Giulio Casi, Nicolas Huguenin-Dezot, Kathrin Zuberbühler, Jörg Scheuermann and Dario Neri, Site-Specific Traceless Coupling of Potent Cytotoxic Drugs to Recombinant Antibodies for Pharmacodelivery, Journal of the American Chemical Society, 134, 13, (5887), (2012).
- Giulio Casi and Dario Neri, Antibody–drug conjugates: Basic concepts, examples and future perspectives, Journal of Controlled Release, 161, 2, (422), (2012).
- Kathrin Zuberbühler, Giulio Casi, Gonçalo J. L. Bernardes and Dario Neri, Fucose-specific conjugation of hydrazide derivatives to a vascular-targeting monoclonal antibody in IgG format, Chemical Communications, 48, 56, (7100), (2012).
- Anna Cyranka-Czaja, Sarah Wulhfard, Dario Neri and Jacek Otlewski, Selection and characterization of human antibody fragments specific for psoriasin – A cancer associated protein, Biochemical and Biophysical Research Communications, 419, 2, (250), (2012).
- Katharina Frey, Michael Fiechter, Kathrin Schwager, Benedetta Belloni, Marjam J. Barysch, Dario Neri and Reinhard Dummer, Different patterns of fibronectin and tenascin‐C splice variants expression in primary and metastatic melanoma lesions, Experimental Dermatology, 20, 8, (685-688), (2011).
- Katharina Frey, Andjelija Zivanovic, Kathrin Schwager and Dario Neri, Antibody-based targeting of interferon-alpha to the tumor neovasculature: a critical evaluation, Integrative Biology, 3, 4, (468), (2011).
- Alessandra Villa, Valeria Lovato, Emil Bujak, Sarah Wulhfard, Nadine Pasche and Dario Neri, A novel synthetic naïve human antibody library allows the isolation of antibodies against a new epitope of oncofetal fibronectin, mAbs, 3, 3, (264), (2011).
- A Palumbo, F Hauler, P Dziunycz, K Schwager, A Soltermann, F Pretto, C Alonso, G F Hofbauer, R W Boyle and D Neri, A chemically modified antibody mediates complete eradication of tumours by selective disruption of tumour blood vessels, British Journal of Cancer, 104, 7, (1106), (2011).
- Nadine Pasche, Janine Woytschak, Sarah Wulhfard, Alessandra Villa, Katharina Frey and Dario Neri, Cloning and characterization of novel tumor-targeting immunocytokines based on murine IL7, Journal of Biotechnology, 154, 1, (84), (2011).
- Tim Fugmann, Beatrice Borgia, Csaba Révész, Mária Godó, Carol Forsblom, Peter Hamar, Harry Holthöfer, Dario Neri and Christoph Roesli, Proteomic identification of vanin-1 as a marker of kidney damage in a rat model of type 1 diabetic nephropathy, Kidney International, 10.1038/ki.2011.116, 80, 3, (272-281), (2011).
- Andrew R M Bradbury, Sachdev Sidhu, Stefan Dübel and John McCafferty, Beyond natural antibodies: the power of in vitro display technologies, Nature Biotechnology, 10.1038/nbt.1791, 29, 3, (245-254), (2011).
- Michael Fiechter, Katharina Frey, Tim Fugmann, Philipp A. Kaufmann and Dario Neri, Comparative in vivo analysis of the atherosclerotic plaque targeting properties of eight human monoclonal antibodies, Atherosclerosis, 214, 2, (325), (2011).
- Anja Baldinger, Bernhard R. Brehm, Petra Richter, Torsten Bossert, Katja Gruen, Khosro Hekmat, Hartwig Kosmehl, Dario Neri, Hans-Reiner Figulla, Alexander Berndt and Marcus Franz, Comparative analysis of oncofetal fibronectin and tenascin-C expression in right atrial auricular and left ventricular human cardiac tissue from patients with coronary artery disease and aortic valve stenosis, Histochemistry and Cell Biology, 135, 5, (427), (2011).
- Kathrin Schwager, Alessandra Villa, Christoph Rösli, Dario Neri, Maria Rösli-Khabas and Gerhard Moser, A comparative immunofluorescence analysis of three clinical-stage antibodies in head and neck cancer, Head & Neck Oncology, 3, 1, (25), (2011).
- Kathrin Schwager, Frank Bootz, Patrick Imesch, Manuela Kaspar, Eveline Trachsel and Dario Neri, The antibody-mediated targeted delivery of interleukin-10 inhibits endometriosis in a syngeneic mouse model, Human Reproduction, 26, 9, (2344), (2011).
- Verena Strassberger, Sabrina Trüssel, Tim Fugmann, Dario Neri and Christoph Roesli, A novel reactive ester derivative of biotin with reduced membrane permeability for in vivo biotinylation experiments, PROTEOMICS, 10, 19, (3544-3548), (2010).
- Tim Fugmann, Dario Neri and Christoph Roesli, DeepQuanTR: MALDI‐MS‐based label‐free quantification of proteins in complex biological samples, PROTEOMICS, 10, 14, (2631-2643), (2010).
- R. Sommavilla, V. Lovato, A. Villa, D. Sgier and D. Neri, Design and construction of a naïve mouse antibody phage display library, Journal of Immunological Methods, 353, 1-2, (31), (2010).
- Flavio Schwarz, Wei Huang, Cishan Li, Benjamin L Schulz, Christian Lizak, Alessandro Palumbo, Shin Numao, Dario Neri, Markus Aebi and Lai-Xi Wang, A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation, Nature Chemical Biology, 6, 4, (264), (2010).
- Marcus Franz, Bernhard R. Brehm, Petra Richter, Katja Gruen, Dario Neri, Hartwig Kosmehl, Khosro Hekmat, Andre Renner, Jan Gummert, Hans R. Figulla and Alexander Berndt, Changes in extra cellular matrix remodelling and re-expression of fibronectin and tenascin-C splicing variants in human myocardial tissue of the right atrial auricle: implications for a targeted therapy of cardiovascular diseases using human SIP format antibodies, Journal of Molecular Histology, 41, 1, (39), (2010).
- Roberto Sommavilla, Nadine Pasche, Eveline Trachsel, Leonardo Giovannoni, Christoph Roesli, Alessandra Villa, Dario Neri and Manuela Kaspar, Expression, engineering and characterization of the tumor-targeting heterodimeric immunocytokine F8-IL12, Protein Engineering, Design and Selection, 10.1093/protein/gzq038, 23, 8, (653-661), (2010).
- Stefanie Pfaffen, Katharina Frey, Irène Stutz, Christoph Roesli and Dario Neri, Tumour-targeting properties of antibodies specific to MMP-1A, MMP-2 and MMP-3, European Journal of Nuclear Medicine and Molecular Imaging, 37, 8, (1559), (2010).
- A. M. Piccinini and K. S. Midwood, DAMPening Inflammation by Modulating TLR Signalling, Mediators of Inflammation, 10.1155/2010/672395, 2010, (1-21), (2010).
- Marta Pedretti, Zoran Rancic, Alex Soltermann, Bernhard A. Herzog, Christoph Schliemann, Mario Lachat, Dario Neri and Philipp A. Kaufmann, Comparative immunohistochemical staining of atherosclerotic plaques using F16, F8 and L19: Three clinical-grade fully human antibodies, Atherosclerosis, 208, 2, (382), (2010).
- D. Sgier, K. Zuberbuehler, S. Pfaffen and D. Neri, Isolation and characterization of an inhibitory human monoclonal antibody specific to the urokinase-type plasminogen activator, uPA, Protein Engineering Design and Selection, 23, 4, (261), (2010).
- Alexander Berndt, Robert Köllner, Petra Richter, Marcus Franz, Astrid Voigt, Angela Berndt, Laura Borsi, Raffaella Giavazzi, Dario Neri and Hartwig Kosmehl, A comparative analysis of oncofetal fibronectin and tenascin-C incorporation in tumour vessels using human recombinant SIP format antibodies, Histochemistry and Cell Biology, 133, 4, (467), (2010).
- Stefanie Pfaffen, Teresa Hemmerle, Marcel Weber and Dario Neri, Isolation and characterization of human monoclonal antibodies specific to MMP-1A, MMP-2 and MMP-3, Experimental Cell Research, 316, 5, (836), (2010).
- Marcus Franz, K. Grün, P. Richter, B. R. Brehm, M. Fritzenwanger, K. Hekmat, D. Neri, J. Gummert, H. R. Figulla, H. Kosmehl, A. Berndt and A. Renner, Extra cellular matrix remodelling after heterotopic rat heart transplantation: gene expression profiling and involvement of ED-A+ fibronectin, alpha-smooth muscle actin and B+ tenascin-C in chronic cardiac allograft rejection, Histochemistry and Cell Biology, 134, 5, (503), (2010).
- Katharina Frey, Christoph Schliemann, Kathrin Schwager, Raffaella Giavazzi, Manfred Johannsen and Dario Neri, The Immunocytokine F8-IL2 Improves the Therapeutic Performance of Sunitinib in a Mouse Model of Renal Cell Carcinoma, The Journal of Urology, 184, 6, (2540), (2010).
- Eleni Bazigou, Sherry Xie, Chun Chen, Anne Weston, Naoyuki Miura, Lydia Sorokin, Ralf Adams, Andrés F. Muro, Dean Sheppard and Taija Makinen, Integrin-α9 Is Required for Fibronectin Matrix Assembly during Lymphatic Valve Morphogenesis, Developmental Cell, 17, 2, (175), (2009).
- J K J Ahlskog, C Schliemann, J Mårlind, U Qureshi, A Ammar, R B Pedley and D Neri, Human monoclonal antibodies targeting carbonic anhydrase IX for the molecular imaging of hypoxic regions in solid tumours, British Journal of Cancer, 10.1038/sj.bjc.6605200, 101, 4, (645-657), (2009).
- Marta Pedretti, Alex Soltermann, Stephan Arni, Walter Weder, Dario Neri and Sven Hillinger, Comparative immunohistochemistry of L19 and F16 in non-small cell lung cancer and mesothelioma: Two human antibodies investigated in clinical trials in patients with cancer, Lung Cancer, 64, 1, (28), (2009).
- Christoph Schliemann, Andrea Wiedmer, Marta Pedretti, Monika Szczepanowski, Wolfram Klapper and Dario Neri, Three clinical-stage tumor targeting antibodies reveal differential expression of oncofetal fibronectin and tenascin-C isoforms in human lymphoma, Leukemia Research, 33, 12, (1718), (2009).
- Sophie Astrof and Richard O. Hynes, Fibronectins in vascular morphogenesis, Angiogenesis, 12, 2, (165), (2009).
- C. Schliemann, A. Palumbo, K. Zuberbuhler, A. Villa, M. Kaspar, E. Trachsel, W. Klapper, H. D. Menssen and D. Neri, Complete eradication of human B-cell lymphoma xenografts using rituximab in combination with the immunocytokine L19-IL2, Blood, 113, 10, (2275), (2009).
- Michael Leiss, Karsten Beckmann, Amparo Girós, Mercedes Costell and Reinhard Fässler, The role of integrin binding sites in fibronectin matrix assembly in vivo, Current Opinion in Cell Biology, 20, 5, (502), (2008).
- K. Zuberbuhler, A. Palumbo, C. Bacci, L. Giovannoni, R. Sommavilla, M. Kaspar, E. Trachsel and D. Neri, A general method for the selection of high-level scFv and IgG antibody expression by stably transfected mammalian cells, Protein Engineering Design and Selection, 22, 3, (169), (2008).
- Na Tian, Jialiang Li, Jinming Shi and Guangchao Sui, From General Aberrant Alternative Splicing in Cancers and Its Therapeutic Application to the Discovery of an Oncogenic DMTF1 Isoform, International Journal of Molecular Sciences, 10.3390/ijms18030191, 18, 3, (191), (2017).
- Matthias Rinderknecht, Alessandra Villa, Kurt Ballmer-Hofer, Dario Neri, Michael Detmar and Cameron Neylon, Phage-Derived Fully Human Monoclonal Antibody Fragments to Human Vascular Endothelial Growth Factor-C Block Its Interaction with VEGF Receptor-2 and 3, PLoS ONE, 10.1371/journal.pone.0011941, 5, 8, (e11941), (2010).
- Lisha Xiang, Ganfeng Xie, Juanjuan Ou, Xing Wei, Feng Pan, Houjie Liang and Andrew Yeudall, The Extra Domain A of Fibronectin Increases VEGF-C Expression in Colorectal Carcinoma Involving the PI3K/AKT Signaling Pathway, PLoS ONE, 10.1371/journal.pone.0035378, 7, 4, (e35378), (2012).
