Preparation and characterization of collagen‐nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications†
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How to cite this article: : Rodrigues SC, Salgado CL, Sahu A, Garcia MP, Fernandes MH, Monteiro FJ. 2013. Preparation and characterization of collagen‐nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications. J Biomed Mater Res Part A 2013:101A:1080–1094.
Abstract
Recent efforts of bone repair focus on development of porous scaffolds for cell adhesion and proliferation. Collagen‐nanohydroxyapatite (HA) scaffolds (70:30; 50:50; and 30:70 mass percentage) were produced by cryogelation technique using 1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide hydrochloride/N‐hydroxysuccinimide as crosslinking agents. A pure collagen scaffold was used as control. Morphology analysis revealed that all cryogels had highly porous structure with interconnective porosity and the nanoHA aggregates were randomly dispersed throughout the scaffold structure. Chemical analysis showed the presence of all major peaks related to collagen and HA in the biocomposites and indicated possible interaction between nanoHA aggregates and collagen molecules. Porosity analysis revealed an enhancement in the surface area as the nanoHA percentage increased in the collagen structure. The biocomposites showed improved mechanical properties as the nanoHA content increased in the scaffold. As expected, the swelling capacity decreased with the increase of nanoHA content. In vitro studies with osteoblasts cells showed that they were able to attach and spread in all cryogels surfaces. The presence of collagen‐nanoHA biocomposites resulted in higher overall cellular proliferation compared to pure collagen scaffold. A statistically significant difference between collagen and collagen‐nanoHA cryogels was observed after 21 day of cell culture. These innovative collagen‐nanoHA cryogels could have potentially appealing application as scaffolds for bone regeneration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Citing Literature
Number of times cited according to CrossRef: 41
- Chunyong Liang, Yongchao Luo, Guodong Yang, Dan Xia, Lei Liu, Xiaomin Zhang and Hongshui Wang, Graphene Oxide Hybridized nHAC/PLGA Scaffolds Facilitate the Proliferation of MC3T3-E1 Cells, Nanoscale Research Letters, 13, 1, (2018).
- Gopal Shankar Krishnakumar, Natalia Gostynska, Massimiliano Dapporto, Elisabetta Campodoni, Monica Montesi, Silvia Panseri, Anna Tampieri, Elizaveta Kon, Maurilio Marcacci, Simone Sprio and Monica Sandri, Evaluation of different crosslinking agents on hybrid biomimetic collagen-hydroxyapatite composites for regenerative medicine, International Journal of Biological Macromolecules, 10.1016/j.ijbiomac.2017.08.076, 106, (739-748), (2018).
- Mehdi Razavi, Sophia Hu and Avnesh S. Thakor, A collagen based cryogel bioscaffold coated with nanostructured polydopamine as a platform for mesenchymal stem cell therapy, Journal of Biomedical Materials Research Part A, 106, 8, (2213-2228), (2018).
- Safdar Ali, Farooq Khurum Shehzad, Inamullah Maitlo, Sultan Valiev, Ghulam Muhyodin and Jun Nie, Binary phase solid‐state photopolymerization behavior of acrylate cryogels under different light sources, Journal of Applied Polymer Science, 135, 37, (2018).
- Sekaran Saravanan, Selvaraj Vimalraj, Palanisamy Thanikaivelan, Sivanantham Banudevi and Geetha Manivasagam, A review on injectable chitosan/beta glycerophosphate hydrogels for bone tissue regeneration, International Journal of Biological Macromolecules, 10.1016/j.ijbiomac.2018.10.014, (2018).
- Abiy Wubneh, Eleni K. Tsekoura, Cagri Ayranci and Hasan Uludağ, Current state of fabrication technologies and materials for bone tissue engineering, Acta Biomaterialia, 10.1016/j.actbio.2018.09.031, (2018).
- G. Ruphuy, M. Souto‐Lopes, D. Paiva, P. Costa, A. E. Rodrigues, F. J. Monteiro, C. L. Salgado, M. H. Fernandes, J. C. Lopes, M. M. Dias and M. F. Barreiro, Supercritical CO2 assisted process for the production of high‐purity and sterile nano‐hydroxyapatite/chitosan hybrid scaffolds, Journal of Biomedical Materials Research Part B: Applied Biomaterials, 106, 3, (965-975), (2017).
- Sylvain Deville, Ice-Templated Materials: Polymers, Ceramics, Metals and Their Composites, Freezing Colloids: Observations, Principles, Control, and Use, 10.1007/978-3-319-50515-2_5, (253-350), (2017).
- B Kaczmarek, A Sionkowska, F J Monteiro, A Carvalho, K Łukowicz and A M Osyczka, Characterization of gelatin and chitosan scaffolds cross-linked by addition of dialdehyde starch, Biomedical Materials, 13, 1, (015016), (2017).
- Li Chen, Zhenxu Wu, Yulai Zhou, Linlong Li, Yu Wang, Zongliang Wang, Yue Chen and Peibiao Zhang, Biomimetic porous collagen/hydroxyapatite scaffold for bone tissue engineering, Journal of Applied Polymer Science, 134, 37, (2017).
- Alina Sionkowska and Sylwia Grabska, Preparation and characterization of 3D collagen materials with magnetic properties, Polymer Testing, 10.1016/j.polymertesting.2017.07.026, 62, (382-391), (2017).
- Yanny Marliana Baba Ismail, Ana Marina Ferreira, Oana Bretcanu, Kenneth Dalgarno and Alicia J. El Haj, Polyelectrolyte multi-layers assembly of SiCHA nanopowders and collagen type I on aminolysed PLA films to enhance cell-material interactions, Colloids and Surfaces B: Biointerfaces, 10.1016/j.colsurfb.2017.07.086, 159, (445-453), (2017).
- Andy H. Choi and Besim Ben-Nissan, Calcium Phosphate Nanocomposites for Biomedical and Dental Applications: Recent Developments, Handbook of Composites from Renewable Materials, (423-450), (2017).
- Akhilesh Kumar Shakya and Umadevi Kandalam, Three-dimensional macroporous materials for tissue engineering of craniofacial bone, British Journal of Oral and Maxillofacial Surgery, 10.1016/j.bjoms.2017.09.007, (2017).
- Jasper Van Hoorick, Peter Gruber, Marica Markovic, Maximilian Tromayer, Jürgen Van Erps, Hugo Thienpont, Robert Liska, Aleksandr Ovsianikov, Peter Dubruel and Sandra Van Vlierberghe, Cross-Linkable Gelatins with Superior Mechanical Properties Through Carboxylic Acid Modification: Increasing the Two-Photon Polymerization Potential, Biomacromolecules, 10.1021/acs.biomac.7b00905, 18, 10, (3260-3272), (2017).
- Sylvain Deville, Properties and Applications of Ice-Templated Materials, Freezing Colloids: Observations, Principles, Control, and Use, 10.1007/978-3-319-50515-2_7, (439-548), (2017).
- Daniela Predoi, Simona Liliana Iconaru, Madalina Albu, Cristian Catalin Petre and Gabriel Jiga, Physicochemical and antimicrobial properties of silver‐doped hydroxyapatite collagen biocomposite, Polymer Engineering & Science, 57, 6, (537-545), (2017).
- Gopal Shankar Krishnakumar, Natalia Gostynska, Elisabetta Campodoni, Massimiliano Dapporto, Monica Montesi, Silvia Panseri, Anna Tampieri, Elizaveta Kon, Maurilio Marcacci, Simone Sprio and Monica Sandri, Ribose mediated crosslinking of collagen-hydroxyapatite hybrid scaffolds for bone tissue regeneration using biomimetic strategies, Materials Science and Engineering: C, 10.1016/j.msec.2017.03.255, 77, (594-605), (2017).
- Katherine R. Hixon, Tracy Lu and Scott A. Sell, A comprehensive review of cryogels and their roles in tissue engineering applications, Acta Biomaterialia, 10.1016/j.actbio.2017.08.033, 62, (29-41), (2017).
- Mariya V Konovalova, Pavel A Markov, Galina Yu Popova, Ida R Nikitina, Konstantin V Shumikhin, Denis V Kurek, Valery P Varlamov and Sergey V Popov, Prevention of postoperative adhesions by biodegradable cryogels from pectin and chitosan polysaccharides, Journal of Bioactive and Compatible Polymers, 10.1177/0883911517690758, 32, 5, (487-502), (2017).
- N.S. Remya, S. Syama, A. Sabareeswaran and P.V. Mohanan, Investigation of chronic toxicity of hydroxyapatite nanoparticles administered orally for one year in wistar rats, Materials Science and Engineering: C, 10.1016/j.msec.2017.03.076, 76, (518-527), (2017).
- Christiane Laranjo Salgado, Liliana Grenho, Maria Helena Fernandes, Bruno Jorge Colaço and Fernando Jorge Monteiro, Biodegradation, biocompatibility, and osteoconduction evaluation of collagen‐nanohydroxyapatite cryogels for bone tissue regeneration, Journal of Biomedical Materials Research Part A, 104, 1, (57-70), (2015).
- Li Chen, Jingxiao Hu, Jiabing Ran, Xinyu Shen and Hua Tong, Synthesis and cytocompatibility of collagen/hydroxyapatite nanocomposite scaffold for bone tissue engineering, Polymer Composites, 37, 1, (81-90), (2014).
- Ruchi Mishra, Sumrita Bhat and Ashok Kumar, Cryogel Biomaterials for Musculoskeletal Tissue Engineering, Supermacroporous Cryogels, 10.1201/b19676-11, (219-254), (2016).
- Limei Wang and Pishan Yang, Nanostructured scaffold and its bioactive potentials in bone tissue engineering, Nanobiomaterials in Hard Tissue Engineering, 10.1016/B978-0-323-42862-0.00008-0, (241-270), (2016).
- Raphaël F. Canadas, Sandra Pina, Alexandra P. Marques, Joaquim M. Oliveira and Rui L. Reis, Cartilage and Bone Regeneration—How Close Are We to Bedside?, Translating Regenerative Medicine to the Clinic, 10.1016/B978-0-12-800548-4.00007-3, (89-106), (2016).
- Rekha R. Sehgal, Edmund Carvalho and Rinti Banerjee, Mechanically Stiff, Zinc Cross-Linked Nanocomposite Scaffolds with Improved Osteostimulation and Antibacterial Properties, ACS Applied Materials & Interfaces, 10.1021/acsami.6b02740, 8, 22, (13735-13747), (2016).
- Gráinne M. Cunniffe, Caroline M. Curtin, Emmet M. Thompson, Glenn R. Dickson and Fergal J. O’Brien, Content-Dependent Osteogenic Response of Nanohydroxyapatite: An in Vitro and in Vivo Assessment within Collagen-Based Scaffolds, ACS Applied Materials & Interfaces, 10.1021/acsami.6b06596, 8, 36, (23477-23488), (2016).
- Jasper Van Hoorick, Hugo Thienpont, Peter Dubruel and Sandra Van Vlierberghe, Cell Regeneration: Current Knowledge and Evolutions, Surgery of the Spine and Spinal Cord, 10.1007/978-3-319-27613-7_3, (15-63), (2016).
- Brianna C. Thompson, Eoin Murray and Gordon G. Wallace, Graphite Oxide to Graphene. Biomaterials to Bionics, Advanced Materials, 27, 46, (7563-7582), (2015).
- Sandra Pina, Joaquim M. Oliveira and Rui L. Reis, Natural‐Based Nanocomposites for Bone Tissue Engineering and Regenerative Medicine: A Review, Advanced Materials, 27, 7, (1143-1169), (2015).
- Omid Mashinchian, Shahin Bonakdar, Shahriar Sharifi and Morteza Mahmoudi, Stem‐Cell Nanoengineering from Bench to Bed, Stem‐Cell Nanoengineering, (381-396), (2015).
- Jing-xiao Hu, Xuan Cai, Shao-bo Mo, Li Chen, Xin-yu Shen and Hua Tong, Fabrication and characterization of chitosan-silk fibroin/hydroxyapatite composites via in situ precipitation for bone tissue engineering, Chinese Journal of Polymer Science, 10.1007/s10118-015-1710-3, 33, 12, (1661-1671), (2015).
- Rameshwar R. Rao and Jan P. Stegemann, Cell-based approaches to the engineering of vascularized bone tissue, Cytotherapy, 10.1016/j.jcyt.2013.06.005, 15, 11, (1309-1322), (2013).
- Sílvia Vieira, Alain Morais, Joana Silva‐Correia, J. Miguel Oliveira and Rui L. Reis, Natural‐Based Hydrogels: From Processing to Applications, Encyclopedia of Polymer Science and Technology, (1-27), (2017).
- Seda Ceylan, Dilek Göktürk, Didem Demir, M. Damla Özdemir and Nimet Bölgen, Comparison of additive effects on the PVA/starch cryogels: Synthesis, characterization, cytotoxicity, and genotoxicity studies, International Journal of Polymeric Materials and Polymeric Biomaterials, 10.1080/00914037.2017.1383254, (1-10), (2017).
- K R Hixon, C T Eberlin, P U Kadakia, S H McBride-Gagyi, E Jain and S A Sell, A comparison of cryogel scaffolds to identify an appropriate structure for promoting bone regeneration, Biomedical Physics & Engineering Express, 10.1088/2057-1976/2/3/035014, 2, 3, (035014), (2016).
- Katherine R Hixon, Christopher T Eberlin, Tracy Lu, Sydney M Neal, Natasha D Case, Sarah H McBride-Gagyi and Scott A Sell, The calcification potential of cryogel scaffolds incorporated with various forms of hydroxyapatite for bone regeneration, Biomedical Materials, 10.1088/1748-605X/aa5d76, 12, 2, (025005), (2017).
- Joanna Lewandowska-Łańcucka, Sylwia Fiejdasz, Łucja Rodzik, Marcin Kozieł and Maria Nowakowska, Bioactive hydrogel-nanosilica hybrid materials: a potential injectable scaffold for bone tissue engineering, Biomedical Materials, 10.1088/1748-6041/10/1/015020, 10, 1, (015020), (2015).
- Katherine R. Hixon, Marissa N. Carletta, Sydney M. Neal, Muhamed Talovic, Andrew J. Dunn, Koyal Garg and Scott A. Sell, Mineralization and antibacterial potential of bioactive cryogel scaffolds in vitro , International Journal of Polymeric Materials and Polymeric Biomaterials, 10.1080/00914037.2018.1522504, (1-14), (2018).
- Monireh Bakhshpour, Neslihan Idil, Işık Perçin and Adil Denizli, Biomedical Applications of Polymeric Cryogels, Applied Sciences, 10.3390/app9030553, 9, 3, (553), (2019).
