Article
Studies and observations on the growth of mammalian cells in agitated fluid medium
Abstract
Fourteen mammalian cell strains were studied in the ‘stirrer culture’. Fair to excellent growth was obtained with twelve of these strains while two strains failed to show evidence of proliferation. Growth curves, maximal population densities, and recovery following subculture of various cell strains, cultivated in this system, were described. Factors effecting the growth of cells under these conditions were outlined. Growth of cells in methods other than the ‘stirrer culture’ were reported. The practical importance of fluid suspension culture of mammalian cells, the potentialities and the problems involved in scaling such cultures to industrial size were discussed.
Citing Literature
Number of times cited: 17
- R. N. Hull, W. R. Cherry and G. W. Weaver, The origin and characteristics of a pig kidney cell strain, LLC-PK1, In Vitro, 12, 10, (670), (1976).
- George Bölcsföldi and Eva Eliasson, Automatic collection of samples from suspension cell cultures for investigations of prolonged metabolic processes, Analytical Biochemistry, 55, 2, (626), (1973).
- Tatsuhei Kondo, Hidehiko Muragishi and Munehisa Imaizumi, A cell line from a human salivary gland mixed tumor, Cancer, 27, 2, (403-410), (2006).
- R.C. Telling and P.J. Radlett, Large-Scale Cultivation of Mammalian Cells, , 10.1016/S0065-2164(08)70401-5, (91-119), (1970).
- N. Blakebrough, Chapter XIII Design of Laboratory Fermenters, , 10.1016/S0580-9517(08)70144-2, (473-504), (1969).
- Daniel I. C. Wang, Tony J. Sinskey, Robert E. Gerner and Richard P. De Filippi, Effect of centrifugation on the viability of Burkitt lymphoma cells, Biotechnology and Bioengineering, 10, 5, (641-649), (2004).
- Joseph B. Schleicher and Ronald E. Weiss, Application of a multiple surface tissue culture propagator for the production of cell monolayers, virus, and biochemicals, Biotechnology and Bioengineering, 10, 5, (617-624), (2004).
- Robert N. Hull, Donald C. Delong and Irving S. Johnson, Tissue Culture in Virus and Cancer Research, BioScience, 16, 10, (715), (1966).
- , Theoretical and Methodological Basis of Continuous Culture of Microorganisms, (315), (1966).
- J. Řičica, Theoretical and Methodological Basis of Continuous Culture of Microorganisms, (155), (1966).
- William F. Daniels, David A. Parker, Roger W. Johnson and Louis E. Schneider, Controlled pH and oxidation–Reduction potential with a new glass tissue‐culture fermentor, Biotechnology and Bioengineering, 7, 4, (529-553), (2004).
- G.E. Moore and K. Ulrich, Suspension cultures of mammalian cells, Journal of Surgical Research, 5, 6, (270), (1965).
- Bernard E. Cabana, Jeanne C. Prokesch and Gordon S. Christiansen, Study on the biogenesis of catecholamines in pheochromocytoma tissue culture, Archives of Biochemistry and Biophysics, 106, (123), (1964).
- , Advances in Applied Microbiology Volume 4, (213)
- , , (109)
- I. Málek and Z. Fencl, Continuous cultivation of microorganisms, Folia Microbiologica, 6, 3, (192), (1961).
- Roslyn E. Wallace, E.V. Orsi, Hilda B. Ritter and A.W. Moyer, Observations during in vitro growth of cells derived from rabbit kidney, Experimental Cell Research, 25, 3, (671), (1961).
