New possibilities of enzyme synthesis of radioactive nucleotides. II. Phosphoribosylation of radioactive bases of nucleic acids by the catalytic effect of unpurified cell‐free extract of Brevibacterium ammoniagenes
Abstract
The catalytic properties of an unpurified enzyme preparation from cells of Brevibacterium ammoniagenes toward phosphoribosylation of the individual nucleic acid bases are pronouncedly specific, display a high efficiency toward phosphoribosylation of uracil where the con version to uridine‐5′‐phosphate is quantitative, phosphoribosylation of adenine takes place with lower efficiency and guanine and cytosine are practically not phosphoribosylated at all. Using orotic acid‐14C and a complete inhibition of orotidine 5′‐phosphate decarboxylase by means of 6‐azauridine‐5′‐phosphate, a 60 % production of orotidine‐5′‐phosphate‐14C has been achieved. The catalytic efficiency of the enzyme preparation remains preserved even upon substitution of 5‐phosphoribosyl‐1‐pyrophosphate by its direct metabolic precursors.
The possibility of direct application of the unpurified cell‐free extract of the bacteria, the simple composition of the final reaction mixtures and the high degree of production of biologically important compounds makes it possible to employ the method studied for an enzyme synthesis of radioactive uridine‐5′‐phosphate, orotidine‐5′‐phosphate and adenosine‐5′‐phosphate.
Number of times cited: 3
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