Temporal relationship of diploidization and haploidization in the yeast Yarrowia lipolytica
Abstract
By means of special selective conditions we analyzed the temporal relationship of diploidization and haploidization in the yeast Yarrowia lipolytica. These processes could be divided into several steps. Twelve hours after mixing parental strains dissociation products of unstable heterokaryons are selectable. Four hours later karyogamy takes place as shown by isolation of mitotic recombinants. At this time spontaneous haploidization starts which leads to an enrichment of haploid and aneuploid segregants in the cell population. A high portion of chimeric clones which represents the dynamics of segregation were selected at time intervals 16, 23 and 29 hours.
We concluded that an unstable heterokaryon should be a connecting link between sexual and parasexual processes in Y. lipolytica.
Number of times cited: 3
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