Review
Genetic engineering in Yarrowia lipolytica*
*
Plenary lecture presented at the XIIth International Specialized Symposium on Yeast: “Genetics of non‐conventional yeasts”, held in Weimar, GDR, September 13–19, 1987
Abstract
The development of a highly efficient integrative transformation system for the yeast Yarrowia lipolytica has permitted gene cloning by direct complementation. The main features of this system will be presented, as well as the results so far obtained on gene structure. Recent developments of these techniques now concern heterologeous gene expression and protein secretion and will be reviewed.
Citing Literature
Number of times cited: 10
- Farshad Darvishi, Expression of native and mutant extracellular lipases fromarrowia lipolytica in accharomyces cerevisiae, Microbial Biotechnology, 5, 5, (634-641), (2012).
- Catherine Madzak, Claude Gaillardin and Jean-Marie Beckerich, Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review, Journal of Biotechnology, 109, 1-2, (63), (2004).
- Juan Francisco Jiménez‐Bremont, José Ruiz‐Herrera and Angel Dominguez, Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica, FEMS Yeast Research, 1, 3, (195-204), (2006).
- Thomas Juretzek, Hui-Jie Wang, Jean-Marc Nicaud, Stephan Mauersberger and Gerold Barth, Comparison of promoters suitable for regulated overexpression of β-galactosidase in the alkane-utilizing yeastYarrowia lipolytica, Biotechnology and Bioprocess Engineering, 5, 5, (320), (2000).
- Huijie Wang, Annick Le Clainche, Marie‐Thérèse Le Dall, Yves Wache, Yves Pagot, Jean‐Marc Belin, Claudie Gaillardin and Jean‐Marc Nicaud, Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane‐utilizing yeast Yarrowia lipolytica, Yeast, 14, 15, (1373-1386), (1999).
- Gerold Barth and Claude Gaillardin, Physiology and genetics of the dimorphic fungus Yarrowia lipolytica, FEMS Microbiology Reviews, 19, 4, (219-237), (2006).
- Carol S. Enderlin and David M. Ogrydziak, Cloning, nucleotide sequence and functions of XPR6, which codes for a dibasic processing endoprotease from the yeast Yarrowia lipolytica, Yeast, 10, 1, (67-79), (2004).
- Tran Tuan Hiep, Vladimir N. Noskov and Yuri I. Pavlov, Transformation in the methylotrophic yeast Pichia methanolica utilizing homologous ADE1 and heterologous Saccharomyces cerevisiae ADE2 and LEU2 genes as genetic markers, Yeast, 9, 11, (1189-1197), (2004).
- Ronald Bauer, Fritz Paltauf and Sepp D. Kohlwein, Functional expression of bacterial β‐glucuronidase and its use as a reporter system in the yeast Yarrowia lipolytica, Yeast, 9, 1, (71-75), (2004).
- B. Y. Tréton, M. -T. Le Dall and C. M. Gaillardin, Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase, Current Genetics, 22, 5, (345), (1992).
