Article
Partial characterization of α‐mannosidase from Yarrowia lipolytica
Abstract
An exo‐α‐mannosidase (E.C. 3.2.1.24) was characterized in the yeast form of Yarrowia lipolytica. The enzyme located in a crude particulate fraction of the cell extract is under catabolite repression, has an optimum pH of 6.0, a Km of 0.27 mM with p‐nitrophenyl‐α‐D‐mannopyranoside and is partially inhibited by D‐mannose.
The enzyme is not affected by ethylenediaminotetraacetic acid, several cations (only Zn++ increased its activity in a 25%) or sulphydryl reagents and can be partially solubilized by treatment with digitonine.
Citing Literature
Number of times cited: 1
- Ashok V. Bankar, Ameeta R. Kumar and Smita S. Zinjarde, Environmental and industrial applications of Yarrowia lipolytica, Applied Microbiology and Biotechnology, 84, 5, (847), (2009).
