Article
Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica
Abstract
Previous attempts to isolate autonomously replicating sequences (ars) from the dimorphic yeast Yarrowia lipolytica have been unsuccessful. We isolated a Fil− mutant unable to produce hyphae and growing only in a yeast form to facilitate ars isolation. This mutant was transformed with a Y. lipolytica DNA bank and several unstable clones were obtained. Extrachromosomal plasmids were evidenced in yeast recovered in Escherichia coli and characterized by restriction mapping. They were able to retransform Fil− and Fil+ yeast strains at high frequency and transformants displayed a slightly unstable phenotype. The detailed analysis of the plasmids showed that only two different ars sequences had been isolated, each of them corresponding to a unique sequence in the Y. lipolytica genome. We concluded that functional ars sequences that can be cloned on plasmids are rare in this yeast.
Citing Literature
Number of times cited: 20
- Jean‐Marc Nicaud, Yarrowia lipolytica, Yeast, 29, 10, (409-418), (2012).
- Juan Francisco Jiménez-Bremont and José Ruiz-Herrera, Analysis of the transcriptional regulation of YlODC gene from the dimorphic fungus Yarrowia lipolytica, Microbiological Research, 163, 6, (717), (2008).
- Takayoshi Yamane, Hiroaki Sakai, Kazuhiro Nagahama, Takahira Ogawa and Masayoshi Matsuoka, Dissection of centromeric DNA from yeast Yarrowia lipolytica and identification of protein-binding site required for plasmid transmission, Journal of Bioscience and Bioengineering, 105, 6, (571), (2008).
- Catherine Madzak, Claude Gaillardin and Jean-Marie Beckerich, Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review, Journal of Biotechnology, 109, 1-2, (63), (2004).
- Juan Francisco Jiménez‐Bremont, José Ruiz‐Herrera and Angel Dominguez, Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica, FEMS Yeast Research, 1, 3, (195-204), (2006).
- Laurence Vernis, Leonora Poljak, Marion Chasles, Kohji Uchida, Serge Casarégola, Emmanuel Käs, Masayoshi Matsuoka, Claude Gaillardin and Philippe Fournier, Only Centromeres Can Supply the Partition System Required for ARS Function in the Yeast Yarrowia lipolytica, Journal of Molecular Biology, 10.1006/jmbi.2000.4300, 305, 2, (203-217), (2001).
- Thomas Juretzek, Hui-Jie Wang, Jean-Marc Nicaud, Stephan Mauersberger and Gerold Barth, Comparison of promoters suitable for regulated overexpression of β-galactosidase in the alkane-utilizing yeastYarrowia lipolytica, Biotechnology and Bioprocess Engineering, 5, 5, (320), (2000).
- Thomas Petit and Carlos Gancedo, Molecular cloning and characterization of the gene HXK1 encoding the hexokinase from Yarrowia lipolytica, Yeast, 15, 15, (1573-1584), (1999).
- Pedro F. Esteban, Serge Casarégola, Carlos R. Vazquez de Aldana and Francisco Del Rey, Cloning and characterization of the EXG1 gene from the yeast Yarrowia lipolytica, Yeast, 15, 15, (1631-1644), (1999).
- Sven Müller, Thomas Sandal, Peter Kamp‐Hansen and Henrik Dalbøge, Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica, Yeast, 14, 14, (1267-1283), (1998).
- Ching Chuan Chang, Dewey D. Y. Ryu, Cheon Seok Park and Jeong‐Yoon Kim, Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed‐batch process strategy, Biotechnology and Bioengineering, 59, 3, (379-385), (2000).
- Gerold Barth and Claude Gaillardin, Physiology and genetics of the dimorphic fungus Yarrowia lipolytica, FEMS Microbiology Reviews, 19, 4, (219-237), (2006).
- C. W. Yip, C. W. Liew and B. H. Nga, Ribosomal RNA genes ofEndomyces fibuliger: isolation, sequencing and the use of the 26S rRNA gene in integrative transformation ofSaccharomyces cerevisiae for efficient expression of the α-amylase gene ofEndomyces fibuliger, World Journal of Microbiology & Biotechnology, 13, 1, (103), (1997).
- A. Aleksenko and A.J. Clutterbuck, Autonomous Plasmid Replication inAspergillus nidulans:AMA1 and MATE Elements, Fungal Genetics and Biology, 21, 3, (373), (1997).
- Alexei Aleksenko, David Gems and John Clutterbuck, Multiple copies of MATE elements support autonomous plasmid replication in Aspergillus nidulans, Molecular Microbiology, 20, 2, (427-434), (2006).
- William M. Nuttley, Anthony M. Brade, Gary A. Eitzen, John R. Glover, John D. Aitchison, Richard A. Rachubinski and Claude Gaillardin, Rapid identification and characterization of peroxisomal assembly mutants in Yarrowia lipolytica, Yeast, 9, 5, (507-517), (2004).
- C. Hadfield, K.K. Raina, K. Shashi-Menon and R.C. Mount, The expression and performance of cloned genes in yeasts, Mycological Research, 97, 8, (897), (1993).
- Michael A. Romanos, Carol A. Scorer and Jeffrey J. Clare, Foreign gene expression in yeast: a review, Yeast, 8, 6, (423-488), (2004).
- Cécile Tharaud, Anne-Marie Ribet, Claude Costes and Claude Gaillardin, Secretion of human blood coagulation factor XIIIa by the yeast Yarrowia lipolytica, Gene, 121, 1, (111), (1992).
- J.M. Nicaud, P. Fournier, C. La Bonnardière, M. Chasles and C. Gaillardin, Use of ars18 based vectors to increase protein production in Yarrowia lipolytica, Journal of Biotechnology, 19, 2-3, (259), (1991).
