A Simplified Model for the Disruption of Escherichia colṙ. The Effect of Cell Septation
Abstract
A new model for the disruption of Escherichia coli by high‐pressure homogenization has been previously presented. Initial model development assumed a bimodal distribution of effective cell strengths to allow for a possible difference in strength between septated and nonseptated cells. A considerably simpler model is obtained when any difference in strength is neglected and a normal distribution is employed. In this article, the disruption of a culture with an abnormally high septated fraction is examined. Disruption versus pressure curves are predicted using both the bimodal and normal approximations to the strength distribution. An examination of disrupted cultures by optical and electron microscopy suggests that septated cells are indeed weaker, thus implying that a bimodal approximation is strictly correct. However, comparison of the model predictions with the experimental results suggests that the simple normal distribution provides sufficient predictive accuracy even for cultures with a high septated fraction.
Number of times cited: 7
-
2010 International Conference on Computing, Control and Industrial Engineering
Wuhan, China
2010 International Conference on Computing, Control and Industrial Engineering
IEEE
, (2010).
978-0-7695-4026-9
Wu Xue, Zhang
Shaoying and Liu Bin
Study on the Dynamic Pressure Modeling of High-Pressure Jet Homogenization
, (2010).
284
289
5492085
, 10.1109/CCIE.2010.79
http://ieeexplore.ieee.org/document/5492085/
- A.V. Mahulkar, C. Riedel, P.R. Gogate, U. Neis and A.B. Pandit, Effect of dissolved gas on efficacy of sonochemical reactors for microbial cell disruption: Experimental and numerical analysis, Ultrasonics Sonochemistry, 16, 5, (635), (2009).
- Ann M. J. Diels and Chris W. Michiels, High-Pressure Homogenization as a Non-Thermal Technique for the Inactivation of Microorganisms, Critical Reviews in Microbiology, 10.1080/10408410601023516, 32, 4, (201-216), (2008). Proceedings of 2002 American Control Conference Anchorage, AK, USA Proceedings of the 2002 American Control Conference (IEEE Cat. No.CH37301) IEEE , (2002). 0-7803-7298-0 W.J. Kelly, J.C. Miller, M.A. Rogowski and K.R. Muske Model-based control of a high pressure homogenizer , (2002). 2891 2896 vol.4 1025228 , 10.1109/ACC.2002.1025228 http://ieeexplore.ieee.org/document/1025228/
- N.J. Samsatli and N. Shah, An Optimization Based Design Procedure for Biochemical Processes, Food and Bioproducts Processing, 74, 4, (221), (1996).
- N.J. Samsatli and N. Shah, Optimal integrated design of biochemical processes, Computers & Chemical Engineering, 20, (S315), (1996).
- Anton P.J. Middelberg, Process-scale disruption of microorganisms, Biotechnology Advances, 13, 3, (491), (1995).
