Free Access
Synthesis of ketocarotenoids in the seed of Arabidopsis thaliana
Summary
A cDNA coding for a gene necessary for synthesis of ketocarotenoids was cloned from the alga Haematococcus pluvialis and expressed in the seed of Arabidopsis thaliana. The expression of the algal β‐carotene‐oxygenase gene was directed to the seed by use of the 2S, seed storage protein promoter napA. Extracts from seeds of the transgenic plants were clearly red because of accumulation of ketocarotenoids, and free and esterified forms of ketocarotenoids were found in addition to the normal carotenoid composition in the seed. The major ketocarotenoids in the transgenic plants were: 4‐keto‐lutein (3,3′‐dihydroxy‐β‐,ε‐carotene‐4‐one), adonirubin (3‐hydroxy‐β‐,β′‐carotene‐4,4′‐dione) and canthaxanthin (β‐,β′‐carotene‐4,4′‐dione). 4‐Keto‐lutein differs from the more common adonixanthin only in the position of one double bond. To increase the substrate availability for the β‐carotene‐oxygenase, these transformants were crossed with transgenic plants overexpressing a construct of an endogenous phytoene synthase gene, also under the control of the napA promoter. The resulting crossings gave rise to seeds with a 4.6‐fold relative increase of the total pigment, and the three major ketocarotenoids were increased 13‐fold compared to seeds of transgenic plants carrying only the β‐carotene‐oxygenase construct.
Citing Literature
Number of times cited: 49
- Beatriz Fernández-Marín, Fátima Míguez, Leire Méndez-Fernández, Agustí Agut, José M. Becerril, José I. García-Plazaola, Ilse Kranner and Louise Colville, Seed Carotenoid and Tocochromanol Composition of Wild Fabaceae Species Is Shaped by Phylogeny and Ecological Factors, Frontiers in Plant Science, 8, (2017).
- Marilise Nogueira, Eugenia M. A. Enfissi, Maria E. Martínez Valenzuela, Guillaume N. Menard, Richard L. Driller, Peter J. Eastmond, Wolfgang Schuch, Gerhard Sandmann and Paul D. Fraser, Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed, Proceedings of the National Academy of Sciences, (201708349), (2017).
- Sol Maiam Rivera Vélez, Guide for Carotenoid Identification in Biological Samples, Journal of Natural Products, 79, 5, (1473), (2016).
- N. Anila, Daris P. Simon, Arun Chandrashekar, G. A. Ravishankar and R. Sarada, Metabolic engineering of Dunaliella salina for production of ketocarotenoids, Photosynthesis Research, 127, 3, (321), (2016).
- Gemma Farré, Dieter Blancquaert, Teresa Capell, Dominique Van Der Straeten, Paul Christou and Changfu Zhu, Engineering Complex Metabolic Pathways in Plants, Annual Review of Plant Biology, 65, 1, (187), (2014).
- Tohru Ariizumi, Sanae Kishimoto, Ryo Kakami, Takashi Maoka, Hideki Hirakawa, Yutaka Suzuki, Yuko Ozeki, Kenta Shirasawa, Stephane Bernillon, Yoshihiro Okabe, Annick Moing, Erika Asamizu, Christophe Rothan, Akemi Ohmiya and Hiroshi Ezura, Identification of the carotenoid modifying gene 1 as an essential factor in xanthophyll esterification and yellow flower pigmentation in tomato (olanum lycopersicum), The Plant Journal, 79, 3, (453-465), (2014).
- Danxiang Han, Yantao Li and Qiang Hu, Biology and Commercial Aspects of Haematococcus pluvialis, Handbook of Microalgal Culture, (388-405), (2013).
- Jian-cheng ZHANG, Wen-jing ZHOU, Qiang XU, Neng-guo TAO, Jun-li YE, Fei GUO, Juan XU and Xiu-xin DENG, Two Lycopene β-Cyclases Genes from Sweet Orange (Citrus sinensis L. Osbeck) Encode Enzymes With Different Functional Efficiency During the Conversion of Lycopene-to-Provitamin A, Journal of Integrative Agriculture, 12, 10, (1731), (2013).
- M. Águila Ruiz-Sola and Manuel Rodríguez-Concepción, Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway, The Arabidopsis Book, 10, (e0158), (2012).
- Junchao Huang, Yujuan Zhong, Gerhard Sandmann, Jin Liu and Feng Chen, Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants, Planta, 236, 2, (691), (2012).
- Mi-Jeong Ahn, Seol Ah Noh, Sun-Hwa Ha, Kyoungwhan Back, Shin Woo Lee and Jung Myung Bae, Production of ketocarotenoids in transgenic carrot plants with an enhanced level of β-carotene, Plant Biotechnology Reports, 6, 2, (133), (2012).
- Nguyen Thi Lam Hai, Jun-ichiro Masuda, Ikuo Miyajima, Nguyen Quoc Thien, Narges Mojtahedi, Michikazu Hiramatsu, Jong-Hwa Kim and Hiroshi Okubo, Involvement of Carotenoid Cleavage Dioxygenase 4 Gene in Tepal Color Change in Lilium brownii var. colchesteri, Journal of the Japanese Society for Horticultural Science, 81, 4, (366), (2012).
- Y.-J. Zhong, J.-C. Huang, J. Liu, Y. Li, Y. Jiang, Z.-F. Xu, G. Sandmann and F. Chen, Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis, Journal of Experimental Botany, 62, 10, (3659), (2011).
- Pejman Azadi, Ntui Valentaine Otang, Dong Poh Chin, Ikuo Nakamura, Masaki Fujisawa, Hisashi Harada, Norihiko Misawa and Masahiro Mii, Metabolic engineering of Lilium × formolongi using multiple genes of the carotenoid biosynthesis pathway, Plant Biotechnology Reports, 4, 4, (269), (2010).
- K. K. Namitha and P. S. Negi, Chemistry and Biotechnology of Carotenoids, Critical Reviews in Food Science and Nutrition, 10.1080/10408398.2010.499811, 50, 8, (728-760), (2010).
- Gemma Farré, Georgina Sanahuja, Shaista Naqvi, Chao Bai, Teresa Capell, Changfu Zhu and Paul Christou, Travel advice on the road to carotenoids in plants, Plant Science, 179, 1-2, (28), (2010).
- Norihiko Misawa, Carotenoids, Comprehensive Natural Products II, 10.1016/B978-008045382-8.00009-5, (733-753), (2010).
- Yves Lemoine and Benoît Schoefs, Secondary ketocarotenoid astaxanthin biosynthesis in algae: a multifunctional response to stress, Photosynthesis Research, 106, 1-2, (155), (2010).
- Ji-Eun Kim, Kimberly M. Cheng, Neal E. Craft, Björn Hamberger and Carl J. Douglas, Over-expression of Arabidopsis thaliana carotenoid hydroxylases individually and in combination with a β-carotene ketolase provides insight into in vivo functions, Phytochemistry, 71, 2-3, (168), (2010).
- Norihiko MISAWA, Production of Carotenoids with Transgenic Plants, Oleoscience, 9, 9, (385), (2009).
- Masaki Fujisawa, Eiji Takita, Hisashi Harada, Nozomu Sakurai, Hideyuki Suzuki, Kanji Ohyama, Daisuke Shibata and Norihiko Misawa, Pathway engineering of Brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation, Journal of Experimental Botany, 60, 4, (1319), (2009).
- Hisashi Harada, Masaki Fujisawa, Maki Teramoto, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata and Norihiko Misawa, Simple functional analysis of key genes involved in astaxanthin biosynthesis using Arabidopsis cultured cells, Plant Biotechnology, 26, 1, (81), (2009).
- Carlo Rosati, Gianfranco Diretto and Giovanni Giuliano, Biosynthesis and Engineering of Carotenoids and Apocarotenoids in Plants: State of the Art and Future Prospects, Biotechnology and Genetic Engineering Reviews, 26, 1, (139), (2009).
- Changfu Zhu, Shaista Naqvi, Teresa Capell and Paul Christou, Metabolic engineering of ketocarotenoid biosynthesis in higher plants, Archives of Biochemistry and Biophysics, 483, 2, (182), (2009).
- Norihiko Misawa, Pathway engineering of plants toward astaxanthin production, Plant Biotechnology, 26, 1, (93), (2009).
- Wayne L. Morris, Laurence J. M. Ducreux, Glenn J. Bryan and Mark A. Taylor, Molecular Dissection of Sensory Traits in the Potato Tuber, American Journal of Potato Research, 10.1007/s12230-008-9031-5, 85, 4, (286-297), (2008).
- M. Vila, I. Couso and R. León, Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase, Process Biochemistry, 43, 10, (1147), (2008).
- Yujuan Zhong, Junchao Huang and Feng Chen, Synthesis of astaxanthin in the leaves of Arabidopsis Thaliana, Journal of Biotechnology, 136, (S65), (2008).
- Giovanni Giuliano, Raffaela Tavazza, Gianfranco Diretto, Peter Beyer and Mark A. Taylor, Metabolic engineering of carotenoid biosynthesis in plants, Trends in Biotechnology, 26, 3, (139), (2008).
- Jayaraman Jayaraj, Robert Devlin and Zamir Punja, Metabolic engineering of novel ketocarotenoid production in carrot plants, Transgenic Research, 10.1007/s11248-007-9120-0, 17, 4, (489-501), (2007).
- Xiangjun Zhou, Joyce Van Eck and Li Li, Use of the cauliflower Or gene for improving crop nutritional quality, , 10.1016/S1387-2656(08)00006-9, (171-190), (2008).
- Shan Lu and Li Li, Carotenoid Metabolism: Biosynthesis, Regulation, and Beyond, Journal of Integrative Plant Biology, 50, 7, (778-785), (2008).
- Junchao Huang, Jin Liu, Yantao Li and Feng Chen, ISOLATION AND CHARACTERIZATION OF THE PHYTOENE DESATURASE GENE AS A POTENTIAL SELECTIVE MARKER FOR GENETIC ENGINEERING OF THE ASTAXANTHIN‐PRODUCING GREEN ALGA CHLORELLA ZOFINGIENSIS (CHLOROPHYTA), Journal of Phycology, 44, 3, (684-690), (2008).
- Yantao Li, Milton Sommerfeld, Feng Chen and Qiang Hu, Consumption of oxygen by astaxanthin biosynthesis: A protective mechanism against oxidative stress in Haematococcus pluvialis (Chlorophyceae), Journal of Plant Physiology, 165, 17, (1783), (2008).
- Rosa León, Inmaculada Couso and Emilio Fernández, Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii, Journal of Biotechnology, 130, 2, (143), (2007).
- Tanja Gerjets, Manuela Sandmann, Changfu Zhu and Gerhard Sandmann, Metabolic engineering of ketocarotenoid biosynthesis in leaves and flowers of tobacco species, Biotechnology Journal, 2, 10, (1263-1269), (2007).
- Sakae Suzuki, Masahiro Nishihara, Takashi Nakatsuka, Norihiko Misawa, Isao Ogiwara and Saburo Yamamura, Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway, Plant Cell Reports, 26, 7, (951), (2007).
- Changfu Zhu, Tanja Gerjets and Gerhard Sandmann, Nicotiana glauca engineered for the production of ketocarotenoids in flowers and leaves by expressing the cyanobacterial crtO ketolase gene, Transgenic Research, 16, 6, (813), (2007).
- Seon-Kang Choi, Satoru Matsuda, Takayuki Hoshino, Xue Peng and Norihiko Misawa, Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli, Applied Microbiology and Biotechnology, 72, 6, (1238), (2006).
- Wayne L. Morris, Laurence J.M. Ducreux, Paul D. Fraser, Steve Millam and Mark A. Taylor, Engineering ketocarotenoid biosynthesis in potato tubers, Metabolic Engineering, 10.1016/j.ymben.2006.01.001, 8, 3, (253-263), (2006).
- Gerhard Sandmann, Susanne Römer and Paul D. Fraser, Understanding carotenoid metabolism as a necessity for genetic engineering of crop plants, Metabolic Engineering, 8, 4, (291), (2006).
- Dean DellaPenna and Barry J. Pogson, VITAMIN SYNTHESIS IN PLANTS: Tocopherols and Carotenoids, Annual Review of Plant Biology, 57, 1, (711), (2006).
- Patricia Botella‐Pavía and Manuel Rodríguez‐Concepción, Carotenoid biotechnology in plants for nutritionally improved foods, Physiologia Plantarum, 126, 3, (369-381), (2006).
- Kerry L. Taylor, Anika E. Brackenridge, Melané A. Vivier and Anita Oberholster, High-performance liquid chromatography profiling of the major carotenoids in Arabidopsis thaliana leaf tissue, Journal of Chromatography A, 10.1016/j.chroma.2006.04.033, 1121, 1, (83-91), (2006).
- CRISPIN A. HOWITT and BARRY J. POGSON, Carotenoid accumulation and function in seeds and non‐green tissues, Plant, Cell & Environment, 29, 3, (435-445), (2006).
- Francis X. Cunningham and Elisabeth Gantt, A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalis, The Plant Journal, 41, 3, (478-492), (2005).
- Mark Taylor and Gavin Ramsay, Carotenoid biosynthesis in plant storage organs: recent advances and prospects for improving plant food quality, Physiologia Plantarum, 124, 2, (143-151), (2005).
- Susanne Römer and Paul D. Fraser, Recent advances in carotenoid biosynthesis, regulation and manipulation, Planta, 221, 3, (305), (2005).
- Louise Ralley, Eugenia M.A. Enfissi, Norihiko Misawa, Wolfgang Schuch, Peter M. Bramley and Paul D. Fraser, Metabolic engineering of ketocarotenoid formation in higher plants, The Plant Journal, 39, 4, (477-486), (2004).
