Volume 13, Issue 7

A simple method for the calculation of microsatellite genotype distances irrespective of ploidy level

RUŽICA BRUVO

Department of Evolutionary Biology, Institute for Animal Evolution and Ecology, Westphalian Wilhelms‐University, Hüfferstr. 1, 48149 Münster, Germany

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NICOLAAS K. MICHIELS

Department of Evolutionary Biology, Institute for Animal Evolution and Ecology, Westphalian Wilhelms‐University, Hüfferstr. 1, 48149 Münster, Germany

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THOMAS G. D’SOUZA

Department of Evolutionary Biology, Institute for Animal Evolution and Ecology, Westphalian Wilhelms‐University, Hüfferstr. 1, 48149 Münster, Germany

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HINRICH SCHULENBURG

Corresponding Author

Department of Evolutionary Biology, Institute for Animal Evolution and Ecology, Westphalian Wilhelms‐University, Hüfferstr. 1, 48149 Münster, Germany

Hinrich Schulenburg. Fax: + 49 251 8324668; E‐mail: hschulen@uni‐muenster.deSearch for more papers by this author
First published: 17 May 2004
Citations: 288

Abstract

Microsatellites are powerful molecular markers, used commonly to estimate intraspecific genetic distances. With the exception of band sharing similarity index, available distance measures were developed specifically for diploid organisms and are unsuited for comparisons of polyploids. Here, we present a simple method for calculation of microsatellite genotype distances, which takes into account mutation processes and permits comparison of individuals with different ploidy levels. This method should provide a valuable tool for intraspecific analyses of polyploid organisms, which are widespread among plants and some animal taxa. An illustration is given using data from the planarian flatworm Schmidtea polychroa (Platyhelminthes).

Number of times cited according to CrossRef: 288

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