Open Access
Gene stacking in transgenic plants – the challenge for 21st century plant biotechnology
Summary
One of the major technical hurdles impeding the advance of plant genetic engineering and biotechnology is the fact that the expression or manipulation of multiple genes in plants is still difficult to achieve. Although a small proportion of commercial genetically modified (GM) crops present ‘stacked’ or ‘pyramided’ traits, only a handful of products have been developed by introducing three or more novel genes. On the research front, a variety of conventional and more novel methods have been employed to introduce multiple genes into plants, but all techniques suffer from certain drawbacks. In this review, the potential and problems of these various techniques and strategies are discussed, and the prospects for improving these technologies in the future are presented.
Citing Literature
Number of times cited: 194
- Trang T.T. Le, Brett Williams and Sagadevan G. Mundree, An osmotin from the resurrection plant Tripogon loliiformis () confers tolerance to multiple abiotic stresses in transgenic rice, Physiologia Plantarum, 162, 1, (13-34), (2017).
- Fengjun Wang, Junli Feng, Sudan Ye, Hannian Huang and Xianglin Zhang, Development of a multiplex fluorescence quantitative PCR for detection of genetically modified organisms, Biologia, (2018).
- Huaijun Si, Ning Zhang, Xun Tang, Jiangwei Yang, Yikai Wen, Li Wang and Xiangyan Zhou, Transgenic Research in Tuber and Root Crops, Genetic Engineering of Horticultural Crops, 10.1016/B978-0-12-810439-2.00011-8, (225-248), (2018).
- Eric Poliner, Jane A. Pulman, Krzysztof Zienkiewicz, Kevin Childs, Christoph Benning and Eva M. Farré, A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long‐chain polyunsaturated fatty acid production, Plant Biotechnology Journal, 16, 1, (298-309), (2017).
- Amir Hameed, Muhammad Nouman Tahir, Shaheen Asad, Rakhshanda Bilal, Joyce Van Eck, Georg Jander and Shahid Mansoor, RNAi-Mediated Simultaneous Resistance Against Three RNA Viruses in Potato, Molecular Biotechnology, 59, 2-3, (73), (2017).
- Aristidis M. Tsatsakis, Muhammad Amjad Nawaz, Demetrios Kouretas, Georgios Balias, Kai Savolainen, Victor A. Tutelyan, Kirill S. Golokhvast, Jeong Dong Lee, Seung Hwan Yang and Gyuhwa Chung, Environmental impacts of genetically modified plants: A review, Environmental Research, 10.1016/j.envres.2017.03.011, 156, (818-833), (2017).
- Usha Kiran, Athar Ali, Kamaluddin and Malik Zainul Abdin, Metabolic Engineering of Secondary Plant Metabolism, Plant Biotechnology: Principles and Applications, 10.1007/978-981-10-2961-5_6, (173-190), (2017).
- Dieter Blancquaert, Hans Steur, Xavier Gellynck and Dominique Der Straeten, Metabolic engineering of micronutrients in crop plants, Annals of the New York Academy of Sciences, 1390, 1, (59-73), (2016).
- Jae‐Heung Ko, Won‐Chan Kim, Jin‐Seong Cho, Young‐Im Choi, Eung‐Jun Park, Jong Hee Im, Okran Han, Daniel Keathley and Kyung‐Hwan Han, EliteTree™: an advanced biomass tree crop technology that features greater wood density and accelerated stem growth, Biofuels, Bioproducts and Biorefining, 11, 3, (521-533), (2017).
- Madoka Kudo, Satoshi Kidokoro, Takuya Yoshida, Junya Mizoi, Daisuke Todaka, Alisdair R. Fernie, Kazuo Shinozaki and Kazuko Yamaguchi‐Shinozaki, Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants, Plant Biotechnology Journal, 15, 4, (458-471), (2016).
- Darren Plett, Trevor Garnett and Mamoru Okamoto, Molecular genetics to discover and improve nitrogen use efficiency in crop plants, Plant Macronutrient Use Efficiency, 10.1016/B978-0-12-811308-0.00006-5, (93-122), (2017).
- Siddhesh B. Ghag, Host induced gene silencing, an emerging science to engineer crop resistance against harmful plant pathogens, Physiological and Molecular Plant Pathology, 10.1016/j.pmpp.2017.10.003, 100, (242-254), (2017).
- Yujiao Liu, Yaxi Liu, Yong Zhou, Charlene Wight, Zhien Pu, Pengfei Qi, Qiantao Jiang, Mei Deng, Zaoxia Wang, Yuming Wei, Wenguang Cao, Dengcai Liu, Youliang Zheng, Chunji Liu, Judith Frégeau-Reid and Jirui Wang, Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta, Euphytica, 213, 1, (2017).
- Madhu Kamle, Pradeep Kumar, Jayanta Kumar Patra and Vivek K. Bajpai, Current perspectives on genetically modified crops and detection methods, 3 Biotech, 10.1007/s13205-017-0809-3, 7, 3, (2017).
- D. D. Songstad, J. F. Petolino, D. F. Voytas and N. A. Reichert, Genome Editing of Plants, Critical Reviews in Plant Sciences, 10.1080/07352689.2017.1281663, 36, 1, (1-23), (2017).
- Siddanna Savadi, Nemappa Lambani, Prem Lal Kashyap and Deepak Singh Bisht, Genetic engineering approaches to enhance oil content in oilseed crops, Plant Growth Regulation, 83, 2, (207), (2017).
- Vallabhi Ghorecha, Yun Zheng, Li Liu, Ramanjulu Sunkar and N. S. R. Krishnayya, MicroRNA dynamics in a wild and cultivated species of Convolvulaceae exposed to drought stress, Physiology and Molecular Biology of Plants, 23, 2, (291), (2017).
- Thammineni Chakradhar, Srikrishna Mahanty, Ramesha A. Reddy, Kummari Divya, Palakolanu Sudhakar Reddy and Malireddy K. Reddy, Biotechnological Perspective of Reactive Oxygen Species (ROS)-Mediated Stress Tolerance in Plants, Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress, 10.1007/978-981-10-5254-5_3, (53-87), (2017).
- M. S. Parvathi and Karaba N. Nataraja, Simultaneous Expression of Abiotic Stress-Responsive Genes: An Approach to Improve Multiple Stress Tolerance in Crops, Plant Tolerance to Individual and Concurrent Stresses, 10.1007/978-81-322-3706-8_10, (151-163), (2017).
- Shreeparna Ganguly, Gourab Ghosh, Arnab Purohit, Rohini Sreevathsa, Rituparna Kundu Chaudhuri and Dipankar Chakraborti, Effective Screening of Transgenic Pigeonpea in Presence of Negative Selection Agents, Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, (2017).
- Mostafa Aboulela, Yuji Tanaka, Kohji Nishimura, Shoji Mano, Mikio Nishimura, Sumie Ishiguro, Tetsuya Kimura, Tsuyoshi Nakagawa and Carl Ng, Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research, PLOS ONE, 12, 5, (e0177889), (2017).
- Sandra Fresquet-Corrales, Edelín Roque, Alejandro Sarrión-Perdigones, Maricruz Rochina, María P. López-Gresa, Huertas M. Díaz-Mula, José M. Bellés, Francisco Tomás-Barberán, José P. Beltrán, Luis A. Cañas and Changjie Xu, Metabolic engineering to simultaneously activate anthocyanin and proanthocyanidin biosynthetic pathways in Nicotiana spp., PLOS ONE, 12, 9, (e0184839), (2017).
- Wen Zhi Wang, Ben Peng Yang, Xiao Yan Feng, Zheng Ying Cao, Cui Lian Feng, Jun Gang Wang, Guo Ru Xiong, Lin Bo Shen, Jun Zeng, Ting Ting Zhao and Shu Zhen Zhang, Development and Characterization of Transgenic Sugarcane with Insect Resistance and Herbicide Tolerance, Frontiers in Plant Science, 8, (2017).
- Jiaojiao Ren, Penghao Wu, Benjamin Trampe, Xiaolong Tian, Thomas Lübberstedt and Shaojiang Chen, Novel technologies in doubled haploid line development, Plant Biotechnology Journal, 15, 11, (1361-1370), (2017).
- Hyemin Lim, Hyun-Ju Hwang, A-Ram Kim, Man-Ho Cho, Hyeonso Ji, Chang-Kug Kim, Seung Uk Ji, Jung-Il Cho, Soo-Chul Park and Gang-Seob Lee, A simple, rapid and systematic method for the developed GM rice analysis, Plant Biotechnology Reports, 10, 1, (25), (2016).
- Frøydis T. Gillund, Anne I. Myhr, Audrun Utskarpen and Angelika Hilbeck, Stakeholder views on issues to consider when assessing the sustainability of genetically modified potato, International Journal of Agricultural Sustainability, 14, 3, (357), (2016).
- Zhongqiu Teng, Ye Shen, Jing Li, Zhongping Lin, Min Chen, Min Wang, Man Li, Hongran Dong and Luqi Huang, Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein, Molecules, 21, 12, (1134), (2016).
- Jiajia Hou, Pingping Jiang, Shoumei Qi, Ke Zhang, Qiuxia He, Changzheng Xu, Zhaohua Ding, Kewei Zhang, Kunpeng Li and Pradeep K. Agarwal, Isolation and Functional Validation of Salinity and Osmotic Stress Inducible Promoter from the Maize Type-II H+-Pyrophosphatase Gene by Deletion Analysis in Transgenic Tobacco Plants, PLOS ONE, 11, 4, (e0154041), (2016).
- Julia Lambret-Frotté, Sinara Artico, Sarah Muniz Nardeli, Fernando Fonseca, Osmundo Brilhante Oliveira-Neto, Maria Fatima Grossi-de-Sá, Marcio Alves-Ferreira and L. Lukens, Promoter isolation and characterization of GhAO-like1, a Gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs, Genome, 59, 1, (23), (2016).
- Wentao Xu, PCR Methods for Detecting GM Crops and Food in Agriculture and the Food Chain: A Review, Functional Nucleic Acids Detection in Food Safety, 10.1007/978-981-10-1618-9_14, (323-342), (2016).
- Weichang Yu, Yuan‐Yeu Yau and James A. Birchler, Plant artificial chromosome technology and its potential application in genetic engineering, Plant Biotechnology Journal, 14, 5, (1175-1182), (2015).
- Joseph F. Petolino and Sandeep Kumar, Transgenic trait deployment using designed nucleases, Plant Biotechnology Journal, 14, 2, (503-509), (2015).
- M. Dutt, L. Erpen, G. Ananthakrishnan, G. A. Barthe, R. H. Brlansky, I. B. Maiti and J. W. Grosser, Comparative expression analysis of five caulimovirus promoters in citrus, Plant Cell, Tissue and Organ Culture (PCTOC), 126, 2, (229), (2016).
- Ayushi Kamthan, Abira Chaudhuri, Mohan Kamthan and Asis Datta, Genetically modified (GM) crops: milestones and new advances in crop improvement, Theoretical and Applied Genetics, 129, 9, (1639), (2016).
- Fred Rook, Metabolic Engineering of Chemical Defence Pathways in Plant Disease Control, Plant Pathogen Resistance Biotechnology, (71-89), (2016).
- Olaf Czarnecki, Anthony C. Bryan, Sara S. Jawdy, Xiaohan Yang, Zong-Ming Cheng, Jin-Gui Chen and Gerald A. Tuskan, Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana, Plant Methods, 12, 1, (2016).
- Amarjeet Kumar Singh, Kumar Paritosh, Uma Kant, Pradeep Kumar Burma, Deepak Pental and Xianlong Zhang, High Expression of Cry1Ac Protein in Cotton (Gossypium hirsutum) by Combining Independent Transgenic Events that Target the Protein to Cytoplasm and Plastids, PLOS ONE, 11, 7, (e0158603), (2016).
- Jeanette Orbegozo, Maria Lupe Roman, Cristina Rivera, Soledad Gamboa, Jose Carlos Tovar, Gregory A. Forbes, Hannele Lindqvist-Kreuze, Jan F. Kreuze and Marc Ghislain, Rpi-blb2 gene from Solanum bulbocastanum confers extreme resistance to late blight disease in potato, Plant Cell, Tissue and Organ Culture (PCTOC), 125, 2, (269), (2016).
- , Combining Selective Pressures to Enhance the Durability of Disease Resistance Genes, Frontiers in Plant Science, 7, (2016).
- Bao‐Rong Lu, Xiao Yang and Norman C. Ellstrand, Fitness correlates of crop transgene flow into weedy populations: a case study of weedy rice in China and other examples, Evolutionary Applications, 9, 7, (857-870), (2016).
- Nathaniel D. Graham, Jon P. Cody, Nathan C. Swyers, Morgan E. McCaw, Changzeng Zhao and James A. Birchler, Engineered Minichromosomes in Plants, , 10.1016/bs.ircmb.2015.05.002, (63-119), (2015).
- Ri-He Peng, Biao Shi, Xiao-Yan Fu, Yong-Sheng Tian, Wei Zhao, Bo Zhu, Jing Xu, Hong-Juan Han, Zhong-Yi Yuan, Kai-Hong Liu and Quan-Hong Yao, Improving Rice Ability to Degrade Different Polycyclic Aromatic Hydrocarbons Through Multigene Transformation of a Hybrid Dioxygenase System, Plant Molecular Biology Reporter, 33, 4, (1030), (2015).
- Yun-Jeong Han, Yong-Min Kim, Ok-Jin Hwang and Jeong-Il Kim, Characterization of a small constitutive promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene for plant transformation, Plant Cell Reports, 34, 2, (265), (2015).
- D. Cruz and M. Eizaguirre, Do Sesamia nonagrioides (Lepidoptera; Noctuidae) Gravid Females Discriminate Between Bt or Multivitamin Corn Varieties? Role of Olfactory and Visual Cues, Journal of Insect Science, 15, 1, (33), (2015).
- Zhangsheng Zhu, Xiaoxia Xu, Bihao Cao, Changming Chen, Qinghua Chen, Chengbin Xiang, Guoju Chen and Jianjun Lei, Pyramiding of AtEDT1/HDG11 and Cry2Aa2 into pepper (Capsicum annuum L.) enhances drought tolerance and insect resistance without yield decrease, Plant Cell, Tissue and Organ Culture (PCTOC), 120, 3, (919), (2015).
- Shiva Hamzeh, Mostafa Motallebi, Mohammad Reza Zamani and Zahra Moghaddassi Jahromi, Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDD Cis-Acting elements in Transgenic Plants, Iranian Journal of Biotechnology, 13, 3, (1), (2015).
- Sandeep Kumar, Diaa AlAbed, John T. Whitteck, Wei Chen, Sara Bennett, Andrew Asberry, Xiujuan Wang, Daniel DeSloover, Murugesan Rangasamy, Terry R. Wright and Manju Gupta, A combinatorial bidirectional and bicistronic approach for coordinated multi-gene expression in corn, Plant Molecular Biology, 87, 4-5, (341), (2015).
- Qi-chao Zhao, Ming-hong Liu, Xian-wen Zhang, Chao-yang Lin, Qing Zhang and Zhi-cheng Shen, Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene, Journal of Zhejiang University-SCIENCE B, 16, 10, (824), (2015).
- Camilo Mestanza, Ricardo Riegel, Herman Silva and Santiago C. Vásquez, Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa, Electronic Journal of Biotechnology, 10.1016/j.ejbt.2015.07.003, 18, 6, (393-398), (2015).
- He SUN, Zhi-hong LANG, Wei LU, Jie ZHANG, Kang-lai HE, Li ZHU, Min LIN and Da-fang HUANG, Developing transgenic maize (Zea mays L.) with insect resistance and glyphosate tolerance by fusion gene transformation, Journal of Integrative Agriculture, 14, 2, (305), (2015).
- Beth Rasala, Syh-Shiuan Chao, Matthew Pier, Daniel Barrera and Stephen Mayfield, Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae, New Biotechnologies for Increased Energy Security, 10.1201/b18537-14, (187-205), (2015).
- Jon Cody, Nathan Swyers, Morgan McCaw, Nathaniel Graham, Changzeng Zhao and James Birchler, Minichromosomes: Vectors for Crop Improvement, Agronomy, 5, 4, (309), (2015).
- Ahmed M. Rayan, Fikru Nigussie and Louise C. Abbott, Safety Evaluation of Stacked Genetically Modified Corn Event (MON89034 × MON88017) Using Zebrafish as an Animal Model, Food and Nutrition Sciences, 06, 14, (1285), (2015).
- Bei Zhang, Madhusudhan Rapolu, Zhibin Liang, Zhenlin Han, Philip G. Williams and Wei Wen Su, A Dual-Intein Autoprocessing Domain that Directs Synchronized Protein Co-Expression in Both Prokaryotes and Eukaryotes, Scientific Reports, 5, 1, (2015).
- Yafei Wang, Haiyang Zeng, Xu Zhou, Fei Huang, Wei Peng, Lin Liu, Wentao Xiong, Xue Shi and Meizhong Luo, Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences, Plant Cell Reports, 34, 6, (1049), (2015).
- Bernard Perthuis, Jean-Michel Vassal, Catherine Fenouillet and Thierry Leroy, Cry1Ac insecticidal protein levels in genetically modified Coffea canephora Pierre coffee plants were negatively correlated with the growth speed in a field experiment, Euphytica, 202, 3, (373), (2015).
- A. S. Sekan, S. V. Isayenkov and Ya. B. Blume, Development of marker-free transformants by site-specific recombinases, Cytology and Genetics, 49, 6, (397), (2015).
- Ahmed M. Rayan and Louise C. Abbott, Compositional analysis of genetically modified corn events (NK603, MON88017×MON810 and MON89034×MON88017) compared to conventional corn, Food Chemistry, 176, (99), (2015).
- E. Fesenko and R. Edwards, Plant synthetic biology: a new platform for industrial biotechnology, Journal of Experimental Botany, 65, 8, (1927), (2014).
- Jennie Hunter-Cevera, Exploring Plant-Microorganism Relationships for Natural Solutions to Sustainable Agriculture and Food Production, Industrial Biotechnology, 10, 5, (336), (2014).
- L. Righetti, S. Djennane, P. Berthelot, R. Cournol, N. Wilmot, K. Loridon, E. Vergne and E. Chevreau, Elimination of the nptII marker gene in transgenic apple and pear with a chemically inducible R/Rs recombinase, Plant Cell, Tissue and Organ Culture (PCTOC), 117, 3, (335), (2014).
- Mulan Jiang, Bing Guo, Xia Wan, Yangmin Gong, Yinbo Zhang and Chuanjiong Hu, Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia pastoris, Marine Drugs, 12, 12, (1317), (2014).
- Zhifeng Wen, Yazhou Yang, Jinjin Zhang, Xiping Wang, Stacy Singer, Zhongchi Liu, Yingjun Yang, Guohua Yan and Zongrang Liu, Highly interactive nature of flower‐specific enhancers and promoters, and its potential impact on tissue‐specific expression and engineering of multiple genes or agronomic traits, Plant Biotechnology Journal, 12, 7, (951-962), (2014).
- Yoichi Ogawa, Makoto Shirakawa, Yasuko Koumoto, Masaho Honda, Yuki Asami, Yasuhiro Kondo and Ikuko Hara-Nishimura, A simple and reliable multi-gene transformation method for switchgrass, Plant Cell Reports, 33, 7, (1161), (2014).
- Lebin Li, Yi Zhu, Shuangxia Jin and Xianlong Zhang, Pyramiding Bt genes for increasing resistance of cotton to two major lepidopteran pests: Spodoptera litura and Heliothis armigera, Acta Physiologiae Plantarum, 10.1007/s11738-014-1642-5, 36, 10, (2717-2727), (2014).
- Yun Liu, Rui Zhang, Zhongshuai Lian, Shihui Wang and Aaron T. Wright, Yeast cell surface display for lipase whole cell catalyst and its applications, Journal of Molecular Catalysis B: Enzymatic, 106, (17), (2014).
- K Ramawat, Jean-Michel Mérillon and M Ahuja, Regulation of Transgene Expression and Containment in Forest Trees, Tree Biotechnology, 10.1201/b16714-16, (2014).
- Jia Xu, Jun Yang, Xiaoguang Duan, Yueming Jiang and Peng Zhang, Increased expression of native cytosolic Cu/Zn superoxide dismutase and ascorbate peroxidase improves tolerance to oxidative and chilling stresses in cassava (Manihot esculenta Crantz), BMC Plant Biology, 14, 1, (2014).
- Fuhui Xu, Zhixue Liu, Hongyan Xie, Jian Zhu, Juren Zhang, Josef Kraus, Tasja Blaschnig, Reinhard Nehls, Hong Wang and Haibing Yang, Increased Drought Tolerance through the Suppression of ESKMO1 Gene and Overexpression of CBF-Related Genes in Arabidopsis, PLoS ONE, 9, 9, (e106509), (2014).
- Mauriat Mélanie, Le Grégoire, Rozenberg Philippe, Delzon Sylvain, Bréda Nathalie, Clair Bruno, Coutand Catherine, Domec Jean-Christophe, Fourcaud Thierry, Grima-Pettenati Jacqueline, Herrera Raul, Leplé Jean-Charles, Richet Nicolas, Trontin Jean-François and Plomion Christophe, Wood Formation in Trees, Tree Biotechnology, 10.1201/b16714-5, (2014).
- Chunzhi Zhang, Lei Liu, Xiaoxuan Wang, Jack Vossen, Guangcun Li, Tao Li, Zheng Zheng, Jianchang Gao, Yanmei Guo, Richard G. F. Visser, Junming Li, Yuling Bai and Yongchen Du, The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans, Theoretical and Applied Genetics, 127, 6, (1353), (2014).
- Gemma Farré, Dieter Blancquaert, Teresa Capell, Dominique Van Der Straeten, Paul Christou and Changfu Zhu, Engineering Complex Metabolic Pathways in Plants, Annual Review of Plant Biology, 65, 1, (187), (2014).
- Jeremy J. Burdon, Luke G. Barrett, Greg Rebetzke and Peter H. Thrall, Guiding deployment of resistance in cereals using evolutionary principles, Evolutionary Applications, 7, 6, (609-624), (2014).
- Beth A. Rasala, Syh-Shiuan Chao, Matthew Pier, Daniel J. Barrera, Stephen P. Mayfield and Wei Ning Chen, Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae, PLoS ONE, 9, 4, (e94028), (2014).
- Fengjun Wang, Xianglin Zhang, Junli Feng, Zhiqiang Wang and Pengju Wang, Establishment of a quadruplex real-time PCR for screening of genetically modified tomatoes, European Food Research and Technology, 238, 4, (683), (2014).
- Thang Xuan Nguyen, Truong Nguyen, Hussien Alameldin, Benjamin Goheen, Wayne Loescher and Mariam Sticklen, Transgene Pyramiding of theHVA1andmtlDin T3 Maize (Zea maysL.) Plants Confers Drought and Salt Tolerance, along with an Increase in Crop Biomass, International Journal of Agronomy, 2013, (1), (2013).
- Brahma B. Panda, V. Mohan M. Achary, Srikrishna Mahanty and Kamal K. Panda, Plant Adaptation to Abiotic and Genotoxic Stress: Relevance to Climate Change and Evolution, Climate Change and Plant Abiotic Stress Tolerance, (251-294), (2013).
- Dengxia Yi, Lei Cui, Li Wang, Yumei Liu, Mu Zhuang, Yangyong Zhang, Jie Zhang, Zhihong Lang, Zhenxian Zhang, Zhiyuan Fang and Limei Yang, Pyramiding of Bt cry1Ia8 and cry1Ba3 genes into cabbage (Brassica oleracea L. var. capitata) confers effective control against diamondback moth, Plant Cell, Tissue and Organ Culture (PCTOC), 10.1007/s11240-013-0373-4, 115, 3, (419-428), (2013).
- S. Vijayan, N. K. Singh, P. Shukla and P. B. Kirti, Defensin (TvD1) from Tephrosia villosa exhibited strong anti-insect and anti-fungal activities in transgenic tobacco plants, Journal of Pest Science, 86, 2, (337), (2013).
- Suxian Zhu, Anita Duwal, Qi Su, Jack H. Vossen, Richard G. F. Visser and Evert Jacobsen, Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight, Transgenic Research, 22, 2, (315), (2013).
- William M. Ainley, Lakshmi Sastry‐Dent, Mary E. Welter, Michael G. Murray, Bryan Zeitler, Rainier Amora, David R. Corbin, Rebecca R. Miles, Nicole L. Arnold, Tonya L. Strange, Matthew A. Simpson, Zehui Cao, Carley Carroll, Katherine S. Pawelczak, Ryan Blue, Kim West, Lynn M. Rowland, Douglas Perkins, Pon Samuel, Cristie M. Dewes, Liu Shen, Shreedharan Sriram, Steven L. Evans, Edward J. Rebar, Lei Zhang, Phillip D. Gregory, Fyodor D. Urnov, Steven R. Webb and Joseph F. Petolino, Trait stacking via targeted genome editing, Plant Biotechnology Journal, 11, 9, (1126-1134), (2013).
- Frøydis T. Gillund, Lise Nordgaard, Thomas Bøhn, Odd Gunnar Wikmark, Heidi Sjursen Konestabo and Angelika Hilbeck, Selection of Nontarget Testing Organisms for ERA of GM Potato with Increased Resistance to Late Blight, Potato Research, 56, 4, (293), (2013).
- Sunita Patro, Indu B. Maiti and Nrisingha Dey, Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters, Journal of Biotechnology, 163, 3, (311), (2013).
- Erin Egelkrout, Karen McGaughey, Todd Keener, Amberlyn Ferleman, Susan Woodard, Shivakumar Devaiah, Zivko Nikolov, Elizabeth Hood and John Howard, Enhanced Expression Levels of Cellulase Enzymes Using Multiple Transcription Units, BioEnergy Research, 6, 2, (699), (2013).
- Xiaohui Liu, Yingying Shangguan, Jingjie Zhu, Yiqi Lu and Bin Han, The rice OsLTP6 gene promoter directs anther-specific expression by a combination of positive and negative regulatory elements, Planta, 238, 5, (845), (2013).
- Thuirei Jacob Ningshen, R.K. Chaitanya, Prashanth P. Hari, P.S. Vimala Devi and Aparna Dutta-Gupta, Characterization and regulation of Bacillus thuringiensis Cry toxin binding aminopeptidases N (APNs) from non-gut visceral tissues, Malpighian tubule and salivary gland: Comparison with midgut-specific APN in the moth Achaea janata, Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 166, 3-4, (194), (2013).
- Raviraj M. Kalunke, Michela Janni, Stefano Benedettelli and Renato D’Ovidio, Using biolistics and hybridization to combine multiple glycosidase inhibitor transgenes in wheat, Euphytica, 10.1007/s10681-013-0972-5, 194, 3, (443-457), (2013).
- Kathleen D'Halluin, Chantal Vanderstraeten, Jolien Van Hulle, Joanna Rosolowska, Ilse Van Den Brande, Anouk Pennewaert, Kristel D'Hont, Martine Bossut, Derek Jantz, Rene Ruiter and Jean Broadhvest, Targeted molecular trait stacking in cotton through targeted double-strand break induction, Plant Biotechnology Journal, 11, 8, (933), (2013).
- Mario Caccamo and Erich Grotewold, Turning over a new leaf in plant genomics, Genome Biology, 14, 6, (2013).
- Ramu S. Vemanna, Babitha K. Chandrashekar, H. M. Hanumantha Rao, Shailesh K. Sathyanarayanagupta, K. S. Sarangi, Karaba N. Nataraja and M. Udayakumar, A Modified MultiSite Gateway Cloning Strategy for Consolidation of Genes in Plants, Molecular Biotechnology, 53, 2, (129), (2013).
- Philippa J. Barrell, Sathiyamoorthy Meiyalaghan, Jeanne M.E. Jacobs and Anthony J. Conner, Applications of biotechnology and genomics in potato improvement, Plant Biotechnology Journal, 11, 8, (907), (2013).
- Suxian Zhu, Ying Li, Jack H. Vossen, Richard G. F. Visser and Evert Jacobsen, Functional stacking of three resistance genes against Phytophthora infestans in potato, Transgenic Research, 21, 1, (89), (2012).
- Zhang Yang, Damian P. Drew, Bodil Jørgensen, Ulla Mandel, Søren S. Bach, Peter Ulvskov, Steven B. Levery, Eric P. Bennett, Henrik Clausen and Bent L. Petersen, Engineering Mammalian Mucin-typeO-Glycosylation in Plants, Journal of Biological Chemistry, 287, 15, (11911), (2012).
- Allan Brown, rew Paterson and Li Li, Genomics and Breeding in Food Crops, OMICs Technologies, 10.1201/b11534-8, (141-162), (2012).
- He Sun, Zhihong Lang, Li Zhu and Dafang Huang, Acquiring transgenic tobacco plants with insect resistance and glyphosate tolerance by fusion gene transformation, Plant Cell Reports, 31, 10, (1877), (2012).
- Ding-Pei LONG, Bing TAN, Ai-Chun ZHAO, Long-Xia XU and Zhong-Huai XIANG, Progress in Cre/lox site-specific recombination system in higher eukaryotes, Hereditas (Beijing), 10.3724/SP.J.1005.2012.00177, 34, 2, (177-189), (2012).
- B. Koul, R. Yadav, I. Sanyal, S. Sawant, V. Sharma and D.V. Amla, Cis-acting motifs in artificially synthesized expression cassette leads to enhanced transgene expression in tomato (Solanum lycopersicum L.), Plant Physiology and Biochemistry, 61, (131), (2012).
- N. J. Atkinson and P. E. Urwin, The interaction of plant biotic and abiotic stresses: from genes to the field, Journal of Experimental Botany, 10.1093/jxb/ers100, 63, 10, (3523-3543), (2012).
- Narendra Tuteja, Shiv Verma, Ranjan Kumar Sahoo, Sebastian Raveendar and IN Bheema Lingeshwara Reddy, Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern, Journal of Biosciences, 37, 1, (167), (2012).
- Han Zhang, Bao H. Phan, Kai Wang, Barbara J. Artelt, Jiming Jiang, Wayne A. Parrott and R. Kelly Dawe, Stable integration of an engineered megabase repeat array into the maize genome, The Plant Journal, 70, 2, (357-365), (2012).
- A. Raybould, G. Graser, K. Hill and K. Ward, Ecological risk assessments for transgenic crops with combined insect-resistance traits: the example of Bt11 × MIR604 maize, Journal of Applied Entomology, 136, 1-2, (27), (2012).
- Alexander Krichevsky, Adi Zaltsman, Lisa King and Vitaly Citovsky, Expression of complete metabolic pathways in transgenic plants, Biotechnology and Genetic Engineering Reviews, 28, 1, (1), (2012).
- Siddhesh B. Ghag, Upendra K. Singh Shekhawat, Thumballi R. Ganapathi and Keqiang Wu, Petunia Floral Defensins with Unique Prodomains as Novel Candidates for Development of Fusarium Wilt Resistance in Transgenic Banana Plants, PLoS ONE, 7, 6, (e39557), (2012).
- Prakash S. Bisen, Mousumi Debnath and Godavarthi B. K. S. Prasad, Microbes in Agriculture, Microbes, (481-566), (2012).
- Lénaïc Paul, Frédérique Angevin, Cécile Collonnier and Antoine Messéan, Impact of gene stacking on gene flow: the case of maize, Transgenic Research, 21, 2, (243), (2012).
- U. K. S. Shekhawat, T. R. Ganapathi and A. B. Hadapad, Transgenic banana plants expressing small interfering RNAs targeted against viral replication initiation gene display high-level resistance to banana bunchy top virus infection, Journal of General Virology, 93, Pt_8, (1804), (2012).
- Mi-Jin Kim, Jae Kwang Kim, Hye Jeong Kim, Jung Hun Pak, Jai-Heon Lee, Doh-Hoon Kim, Hong Kyu Choi, Ho Won Jung, Jeong-Dong Lee, Young-Soo Chung, Sun-Hwa Ha and Hany A. El-Shemy, Genetic Modification of the Soybean to Enhance the β-Carotene Content through Seed-Specific Expression, PLoS ONE, 7, 10, (e48287), (2012).
- Mercedes Rivero, Nicolás Furman, Nicolás Mencacci, Pablo Picca, Laila Toum, Ezequiel Lentz, Fernando Bravo-Almonacid and Alejandro Mentaberry, Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens, Journal of Biotechnology, 157, 2, (334), (2012).
- Erin Egelkrout, Vidya Rajan and John A. Howard, Overproduction of recombinant proteins in plants, Plant Science, 184, (83), (2012).
- Robert T. Gaeta, Rick E. Masonbrink, Lakshminarasimhan Krishnaswamy, Changzeng Zhao and James A. Birchler, Synthetic Chromosome Platforms in Plants, Annual Review of Plant Biology, 63, 1, (307), (2012).
- Bao‐Rong Lu and Wei Wang, Potential Environmental Impacts of Transgene Flow in Rice with a Particular View on Herbicide Resistance, Plant Gene Containment, (1-25), (2012).
- H. Stroud, S. Otero, B. Desvoyes, E. Ramirez-Parra, S. E. Jacobsen and C. Gutierrez, Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana, Proceedings of the National Academy of Sciences, 10.1073/pnas.1203145109, 109, 14, (5370-5375), (2012).
- Scott F. Hickey, Malathy Sridhar, Alexander J. Westermann, Qian Qin, Pooja Vijayendra, Geoffrey Liou and Ming C. Hammond, Transgene regulation in plants by alternative splicing of a suicide exon, Nucleic Acids Research, 40, 10, (4701), (2012).
- Andrea Gennaro, Paola Forte, Daniela Panichi, Domenico Lafiandra, Mario Augusto Pagnotta, Maria Grazia D’Egidio and Carla Ceoloni, Stacking small segments of the 1D chromosome of bread wheat containing major gluten quality genes into durum wheat: transfer strategy and breeding prospects, Molecular Breeding, 30, 1, (149), (2012).
- Sandra Gouiaa, Habib Khoudi, Eduardo O. Leidi, Jose M. Pardo and Khaled Masmoudi, Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance, Plant Molecular Biology, 79, 1-2, (137), (2012).
- Yu-Fei Sun, Ying Lin, Jun-Hui Zhang, Sui-Ping Zheng, Yan-Rui Ye, Xing-Xiang Liang and Shuang-Yan Han, Double Candida antarctica lipase B co-display on Pichia pastoris cell surface based on a self-processing foot-and-mouth disease virus 2A peptide, Applied Microbiology and Biotechnology, 96, 6, (1539), (2012).
- K. Rajasekaran, J. W. Cary, C. A. Chlan, J. M. Jaynes and D. Bhatnagar, Strategies for Controlling Plant Diseases and Mycotoxin Contamination Using Antimicrobial Synthetic Peptides, Small Wonders: Peptides for Disease Control, 10.1021/bk-2012-1095.ch014, (295-315), (2012).
- Serry Koh, Hongsup Kim, Jinwoo Kim, Eunhye Goo, Yun-Jung Kim, Okhee Choi, Nam-Soo Jwa, Jun Ma, Tomohisa Nagamatsu, Jae Sun Moon and Ingyu Hwang, A novel light-dependent selection marker system in plants, Plant Biotechnology Journal, 9, 3, (348), (2011).
- M. Raj Ahuja, Fate of transgenes in the forest tree genome, Tree Genetics & Genomes, 7, 2, (221), (2011).
- Deng-xia YI, Lei CUI, Yu-mei LIU, Mu ZHUANG, Yang-yong ZHANG, Zhi-yuan FANG and Li-mei YANG, Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth, Agricultural Sciences in China, 10, 11, (1693), (2011).
- Huiquan Zheng, Yang Lei, Shanzhi Lin, Qian Zhang and Zhiyi Zhang, Bidirectionalization of a methyl jasmonate-inducible plant promoter, Biotechnology Letters, 33, 2, (387), (2011).
- Yuanyuan Zhang and Junhong Liu, Transgenic alfalfa plants co-expressing glutathione S-transferase (GST) and human CYP2E1 show enhanced resistance to mixed contaminates of heavy metals and organic pollutants, Journal of Hazardous Materials, 10.1016/j.jhazmat.2011.02.042, 189, 1-2, (357-362), (2011).
- W. Pilacinski, A. Crawford, R. Downey, B. Harvey, S. Huber, P. Hunst, L.K. Lahman, S. MacIntosh, M. Pohl, C. Rickard, L. Tagliani and N. Weber, Plants with genetically modified events combined by conventional breeding: An assessment of the need for additional regulatory data, Food and Chemical Toxicology, 49, 1, (1), (2011).
- Y. Bharathi, S. Vijaya Kumar, I.C. Pasalu, S.M. Balachandran, V.D. Reddy and K.V. Rao, Pyramided rice lines harbouring Allium sativum (asal) and Galanthus nivalis (gna) lectin genes impart enhanced resistance against major sap-sucking pests, Journal of Biotechnology, 152, 3, (63), (2011).
- Elena Maestri and Nelson Marmiroli, Transgenic Plants for Phytoremediation, International Journal of Phytoremediation, 10.1080/15226514.2011.568549, 13, sup1, (264-279), (2011).
- Mangu Venkata Ramana Rao, Chidambaram Parameswari, Rajasekaran Sripriya and Karuppannan Veluthambi, Transgene stacking and marker elimination in transgenic rice by sequential Agrobacterium-mediated co-transformation with the same selectable marker gene, Plant Cell Reports, 30, 7, (1241), (2011).
- Yueju Wang, Yuan-Yeu Yau, Donna Perkins-Balding and James G. Thomson, Recombinase technology: applications and possibilities, Plant Cell Reports, 30, 3, (267), (2011).
- M. Faize, L. Burgos, L. Faize, A. Piqueras, E. Nicolas, G. Barba-Espin, M. J. Clemente-Moreno, R. Alcobendas, T. Artlip and J. A. Hernandez, Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress, Journal of Experimental Botany, 62, 8, (2599), (2011).
- Danny Cullen, Wendy Harwood, Mark Smedley, Howard Davies and Mark Taylor, Comparison of DNA Walking Methods for Isolation of Transgene-Flanking Regions in GM Potato, Molecular Biotechnology, 49, 1, (19), (2011).
- J P Londo, M A Bollman, C L Sagers, E H Lee and L S Watrud, Changes in fitness-associated traits due to the stacking of transgenic glyphosate resistance and insect resistance in Brassica napus L., Heredity, 107, 4, (328), (2011).
- Jacob Pollier, Tessa Moses and Alain Goossens, Combinatorial biosynthesis in plants: A (p)review on its potential and future exploitation, Natural Product Reports, 28, 12, (1897), (2011).
- MAYA MAYROSE, NOLAN C. KANE, ITAY MAYROSE, KATRINA M. DLUGOSCH and LOREN H. RIESEBERG, Increased growth in sunflower correlates with reduced defences and altered gene expression in response to biotic and abiotic stress, Molecular Ecology, 20, 22, (4683-4694), (2011).
- Veerle M.J. Grispen, Henk W.J. Hakvoort, Tijs Bliek, Jos A.C. Verkleij and Henk Schat, Combined expression of the Arabidopsis metallothionein MT2b and the heavy metal transporting ATPase HMA4 enhances cadmium tolerance and the root to shoot translocation of cadmium and zinc in tobacco, Environmental and Experimental Botany, 72, 1, (71), (2011).
- Xiaohua Su, Yanguang Chu, Huan Li, Yingjie Hou, Bingyu Zhang, Qinjun Huang, Zanmin Hu, Rongfeng Huang, Yingchuan Tian and Zhanjiang Liu, Expression of Multiple Resistance Genes Enhances Tolerance to Environmental Stressors in Transgenic Poplar (Populus × euramericana ‘Guariento’), PLoS ONE, 6, 9, (e24614), (2011).
- Manoj K. Dhar, Sanjana Kaul and Jasmeet Kour, Towards the development of better crops by genetic transformation using engineered plant chromosomes, Plant Cell Reports, 30, 5, (799), (2011).
- Zhou Yang, Hao Chen, Wei Tang, Hongxia Hua and Yongjun Lin, Development and characterisation of transgenic rice expressing two Bacillus thuringiensis genes, Pest Management Science, 67, 4, (414-422), (2011).
- Hans-Henno Dörries, Ivonne Remus, Astrid Grönewald, Cordt Grönewald and Kornelia Berghof-Jäger, Development of a qualitative, multiplex real-time PCR kit for screening of genetically modified organisms (GMOs), Analytical and Bioanalytical Chemistry, 396, 6, (2043), (2010).
- Dieter Blancquaert, Sergei Storozhenko, Karen Loizeau, Hans De Steur, Veerle De Brouwer, Jacques Viaene, Stéphane Ravanel, Fabrice Rébeillé, Willy Lambert and Dominique Van Der Straeten, Folates and Folic Acid: From Fundamental Research Toward Sustainable Health, Critical Reviews in Plant Sciences, 10.1080/07352680903436283, 29, 1, (14-35), (2010).
- Yun Lee and Eberhard O. Voit, Mathematical modeling of monolignol biosynthesis in Populus xylem, Mathematical Biosciences, 228, 1, (78), (2010).
- Sun‐Hwa Ha, Ying Shi Liang, Harin Jung, Mi‐Jeong Ahn, Seok‐Cheol Suh, Soon‐Jong Kweon, Dong‐Hern Kim, Young‐Mi Kim and Ju‐Kon Kim, Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm, Plant Biotechnology Journal, 8, 8, (928-938), (2010).
- Fei Ren, Qi-Jun Chen, Min Xie, Li-Juan Li, Wei-Hua Wu, Jia Chen and Xue-Chen Wang, Engineering the K+ uptake regulatory pathway by MultiRound Gateway, Journal of Plant Physiology, 167, 16, (1412), (2010).
- Antje Dietz-Pfeilstetter, Stability of transgene expression as a challenge for genetic engineering, Plant Science, 179, 3, (164), (2010).
- A. Ballester, M. Cervera and L. Peña, Selectable marker-free transgenic orange plants recovered under non-selective conditions and through PCR analysis of all regenerants, Plant Cell, Tissue and Organ Culture (PCTOC), 102, 3, (329), (2010).
- James A. Birchler, Lakshminarasimhan Krishnaswamy, Robert T. Gaeta, Rick E. Masonbrink and Changzeng Zhao, Engineered Minichromosomes in Plants, Critical Reviews in Plant Sciences, 29, 3, (135), (2010).
- A. Alderborn, J. Sundström, D. Soeria-Atmadja, M. Sandberg, H.C. Andersson and U. Hammerling, Genetically modified plants for non-food or non-feed purposes: Straightforward screening for their appearance in food and feed, Food and Chemical Toxicology, 48, 2, (453), (2010).
- Changfu Zhu, Shaista Naqvi, Teresa Capell and Paul Christou, Metabolic engineering of ketocarotenoid biosynthesis in higher plants, Archives of Biochemistry and Biophysics, 483, 2, (182), (2009).
- Johannes Siemens, Plants: Genetic Transformation, Encyclopedia of Industrial Biotechnology, (1-11), (2009).
- P. Stephen Baenziger and Ronald M. Depauw, Wheat Breeding: Procedures and Strategies, Wheat Science and Trade, (273-308), (2009).
- Chandrashekhar P. Joshi, Victor Busov, Shivegowda Thammannagowda, Chung‐Jui Tsai, Amy Brunner and Richard Meilan, Poplars, Compendium of Transgenic Crop Plants, (1-34), (2009).
- Klaus Ammann, Why farming with high tech methods should integrate elements of organic agriculture, New Biotechnology, 25, 6, (378), (2009).
- Craig C. Wood, James R. Petrie, Pushkar Shrestha, Maged P. Mansour, Peter D. Nichols, Allan G. Green and Surinder P. Singh, A leaf‐based assay using interchangeable design principles to rapidly assemble multistep recombinant pathways, Plant Biotechnology Journal, 7, 9, (914-924), (2009).
- S. Naqvi, C. Zhu, G. Farre, K. Ramessar, L. Bassie, J. Breitenbach, D. Perez Conesa, G. Ros, G. Sandmann, T. Capell and P. Christou, Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways, Proceedings of the National Academy of Sciences, 106, 19, (7762), (2009).
- Surendar Reddy Dhadi, Nicholas Krom and Wusirika Ramakrishna, Genome-wide comparative analysis of putative bidirectional promoters from rice, Arabidopsis and Populus, Gene, 429, 1-2, (65), (2009).
- John Davison and Yves Bertheau, Key Issues and Open Questions in GMO Controls, Global Issues in Food Science and Technology, 10.1016/B978-0-12-374124-0.00020-X, (353-365), (2009).
- C Andrew James and Stuart E Strand, Phytoremediation of small organic contaminants using transgenic plants, Current Opinion in Biotechnology, 20, 2, (237), (2009).
- Tahira Fatima, Autar K. Mattoo, Marisela Rivera‐Domínguez, Rosalba Troncoso‐Rojas, Martín‐Ernesto Tiznado‐Hernández and Avtar K. Handa, Tomato, Compendium of Transgenic Crop Plants, (1-46), (2009).
- Sarwan K. Dhir, Hari P. Singh and Seema Dhir, Sweet Potato, Compendium of Transgenic Crop Plants, (157-176), (2009).
- Joann M. Lau and Schuyler S. Korban, Analysis and stability of the Respiratory Syncytial Virus antigen in a T3 generation of transgenic tomato plants, Plant Cell, Tissue and Organ Culture (PCTOC), 96, 3, (335), (2009).
- Garry A. Luke, Helena Escuin, Pablo De Felipe and Martin D. Ryan, 2A to the Fore – Research, Technology and Applications, Biotechnology and Genetic Engineering Reviews, 26, 1, (223), (2009).
- Olga V. Zernova, Anatoliy V. Lygin, Jack M. Widholm and Vera V. Lozovaya, Modification of isoflavones in soybean seeds via expression of multiple phenolic biosynthetic genes, Plant Physiology and Biochemistry, 47, 9, (769), (2009).
- Giandomenico Corrado and Marianthi Karali, Inducible gene expression systems and plant biotechnology, Biotechnology Advances, 10.1016/j.biotechadv.2009.05.006, 27, 6, (733-743), (2009).
- Hong-Gyu Kang, Tae-Woong Bae, Ok-Cheol Jeong, Hyeon-Jin Sun, Pyung-Ok Lim and Hyo-Yeon Lee, Evaluation of Viability, Shedding Pattern, and Longevity of Pollen from Genetically Modified (GM) Herbicide-tolerant and Wild-type Zoysiagrass (Zoysia japonica Steud.), Journal of Plant Biology, 10.1007/s12374-009-9074-2, 52, 6, (630-634), (2009).
- Isabel Taverniers, Nina Papazova, Yves Bertheau, Marc De Loose and Arne Holst-Jensen, Gene stacking in transgenic plants: towards compliance between definitions, terminology, and detection within the EU regulatory framework, Environmental Biosafety Research, 7, 4, (197), (2008).
- E.J. Kok, J. Keijer, G.A. Kleter and H.A. Kuiper, Comparative safety assessment of plant-derived foods, Regulatory Toxicology and Pharmacology, 10.1016/j.yrtph.2007.09.007, 50, 1, (98-113), (2008).
- Jose Luis Ortega, Suman Bagga, Carol Potenza and Champa Sengupta-Gopalan, Genetic Engineering for Forage Quality in Alfalfa, Handbook of New Technologies for Genetic Improvement of Legumes, 10.1201/9781439801352.ch1, (1-18), (2010).
- Lorena Moeller and Kan Wang, Engineering with Precision: Tools for the New Generation of Transgenic Crops, BioScience, 10.1641/B580506, 58, 5, (391-401), (2008).
- Yun Lian, ZhiWei Jia, KangLai He, YunJun Liu, FuPing Song, BaoMin Wang and GuoYing Wang, Transgenic tobacco plants expressing synthetic Cry1Ac and Cry1Ie genes are more toxic to cotton bollworm than those containing one gene, Science Bulletin, 53, 9, (1381), (2008).
- Meriem Benchabane, Marie-Claire Goulet, Cindy Dallaire, Pierre-Luc Côté and Dominique Michaud, Hybrid protease inhibitors for pest and pathogen control – a functional cost for the fusion partners?, Plant Physiology and Biochemistry, 46, 7, (701), (2008).
- Giandomenico Corrado, Stefania Arciello, Paolo Fanti, Luisa Fiandra, Antonio Garonna, Maria Cristina Digilio, Matteo Lorito, Barbara Giordana, Francesco Pennacchio and Rosa Rao, The Chitinase A from the baculovirus AcMNPV enhances resistance to both fungi and herbivorous pests in tobacco, Transgenic Research, 17, 4, (557), (2008).
- Manjit S. Kang, Prasanta K. Subudhi, Niranjan Baisakh and P. M. Priyadarshan, Crop Breeding Methodologies: Classic and Modern, Breeding Major Food Staples, (38-40), (2008).
- Yin ZHOU, Application prospect of new site-specific recombination systems in gene transformation and gene stacking, Hereditas (Beijing), 30, 2, (149), (2008).
- C. Zhu, S. Naqvi, J. Breitenbach, G. Sandmann, P. Christou and T. Capell, Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize, Proceedings of the National Academy of Sciences, 10.1073/pnas.0809737105, 105, 47, (18232-18237), (2008).
- Jun Cao, Anthony M. Shelton and Elizabeth D. Earle, Sequential transformation to pyramid two Bt genes in vegetable Indian mustard (Brassica juncea L.) and its potential for control of diamondback moth larvae, Plant Cell Reports, 27, 3, (479), (2008).
- M. A. Schmidt, P. R. LaFayette, B. A. Artelt and W. A. Parrott, A comparison of strategies for transformation with multiple genes via microprojectile-mediated bombardment, In Vitro Cellular & Developmental Biology - Plant, 10.1007/s11627-007-9099-5, 44, 3, (162-168), (2008).
- Keyan Zhu-Salzman and Ren Sen Zeng, Molecular mechanisms of insect adaptation to plant defense: Lessons learned from a Bruchid beetle, Insect Science, 15, 6, (477), (2008).
- JianGe Wang, XiaoHua Su, LiLi Ji, BingYu Zhang, ZanMin Hu, RongFeng Huang and YingChuan Tian, Multiple transgenes Populus xeuramericana ‘Guariento’ plants obtained by biolistic bombardment, Chinese Science Bulletin, 52, 2, (224), (2007).
- François Torney, Lorena Moeller, Andréa Scarpa and Kan Wang, Genetic engineering approaches to improve bioethanol production from maize, Current Opinion in Biotechnology, 18, 3, (193), (2007).
- Mauritz Venter, Synthetic promoters: genetic control through cis engineering, Trends in Plant Science, 12, 3, (118), (2007).
- David Rodríguez-Lázaro, Bertrand Lombard, Huw Smith, Artur Rzezutka, Martin D'Agostino, Reiner Helmuth, Andreas Schroeter, Burkhard Malorny, Angelika Miko, Beatriz Guerra, John Davison, A. Kobilinsky, Marta Hernández, Yves Bertheau and Nigel Cook, Trends in analytical methodology in food safety and quality: monitoring microorganisms and genetically modified organisms, Trends in Food Science & Technology, 18, 6, (306), (2007).
- Simran Bhullar, Sudipta Datta, Sonia Advani, Suma Chakravarthy, Taru Gautam, Deepak Pental and Pradeep Kumar Burma, Functional analysis of cauliflower mosaic virus 35S promoter: re‐evaluation of the role of subdomains B5, B4 and B2 in promoter activity, Plant Biotechnology Journal, 5, 6, (696-708), (2007).
- N. Arumugam, Vibha Gupta, Arun Jagannath, Arundhati Mukhopadhyay, Akshay K. Pradhan, Pradeep Kumar Burma and Deepak Pental, A passage through in vitro culture leads to efficient production of marker-free transgenic plants in Brassica juncea using the Cre–loxP system, Transgenic Research, 16, 6, (703), (2007).
- Weichang Yu, Fangpu Han and James A Birchler, Engineered minichromosomes in plants, Current Opinion in Biotechnology, 18, 5, (425), (2007).
- Steve Millam, Developments in Transgenic Biology and the Genetic Engineering of Useful Traits, Potato Biology and Biotechnology, 10.1016/B978-044451018-1/50072-5, (669-686), (2007).
- A. De Schrijver, Y. Devos, M. Van den Bulcke, P. Cadot, M. De Loose, D. Reheul and M. Sneyers, Risk assessment of GM stacked events obtained from crosses between GM events, Trends in Food Science & Technology, 18, 2, (101), (2007).
- Qi-Jun Chen, Hai-Meng Zhou, Jia Chen and Xue-Chen Wang, A Gateway-based platform for multigene plant transformation, Plant Molecular Biology, 62, 6, (927), (2006).
- A. Richter, H.-J. Jacobsen, A. de Kathen, G. de Lorenzo, K. Briviba, R. Hain, G. Ramsay and H. Kiesecker, Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera), Plant Cell Reports, 25, 11, (1166), (2006).
- Pablo de Felipe, Garry A. Luke, Lorraine E. Hughes, David Gani, Claire Halpin and Martin D. Ryan, E unum pluribus: multiple proteins from a self-processing polyprotein, Trends in Biotechnology, 24, 2, (68), (2006).
- Robert W. Herdt, Biotechnology in Agriculture, Annual Review of Environment and Resources, 10.1146/annurev.energy.31.031405.091314, 31, 1, (265-295), (2006).
- Om V. Singh, Shivani Ghai, Debarati Paul and Rakesh K. Jain, Genetically modified crops: success, safety assessment, and public concern, Applied Microbiology and Biotechnology, 71, 5, (598), (2006).
- Stephen Morse, Richard Bennett and Yousouf Ismael, Environmental impact of genetically modified cotton in South Africa, Agriculture, Ecosystems & Environment, 117, 4, (277), (2006).
- Marketa Samalova, Mark Fricker and Ian Moore, Ratiometric Fluorescence-Imaging Assays of Plant Membrane Traffic Using Polyproteins, Traffic, 7, 12, (1701), (2006).
- Ling Meng, Meira Ziv and Peggy G. Lemaux, Nature of stress and transgene locus influences transgene expression stability in barley, Plant Molecular Biology, 62, 1-2, (15), (2006).
- Frank Sainsbury, Meriem Benchabane, Marie-Claire Goulet and Dominique Michaud, Multimodal Protein Constructs for Herbivore Insect Control, Toxins, 10.3390/toxins4060455, 4, 12, (455-475), (2012).
- Qichao Zhao, Minghong Liu, Miaomiao Tan, Jianhua Gao, Zhicheng Shen and Mario Soberón, Expression of Cry1Ab and Cry2Ab by a Polycistronic Transgene with a Self-Cleavage Peptide in Rice, PLoS ONE, 10.1371/journal.pone.0110006, 9, 10, (e110006), (2014).
