Volume 12, Issue 3

Spider: An R package for the analysis of species identity and evolution, with particular reference to DNA barcoding

SAMUEL D. J. BROWN

Bio‐Protection Research Centre, PO Box 84, Lincoln University 7647, Canterbury, New Zealand

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RUPERT A. COLLINS

Bio‐Protection Research Centre, PO Box 84, Lincoln University 7647, Canterbury, New Zealand

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STEPHANE BOYER

Department of Ecology, Faculty of Agriculture and Life Sciences, PO Box 84, Lincoln University 7647, Canterbury, New Zealand

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MARIE‐CAROLINE LEFORT

Bio‐Protection Research Centre, PO Box 84, Lincoln University 7647, Canterbury, New Zealand

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JAGOBA MALUMBRES‐OLARTE

Department of Ecology, Faculty of Agriculture and Life Sciences, PO Box 84, Lincoln University 7647, Canterbury, New Zealand

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COR J. VINK

Biosecurity Group, AgResearch, Private Bag 4749, Christchurch 8140, New Zealand

Entomology Research Museum, PO Box 84, Lincoln University, Lincoln 7647, New Zealand

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ROBERT H. CRUICKSHANK

Department of Ecology, Faculty of Agriculture and Life Sciences, PO Box 84, Lincoln University 7647, Canterbury, New Zealand

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First published: 16 January 2012
Citations: 234
Samuel D. J. Brown, Fax: +64 3 325 3844; E‐mail: sam.brown@lincoln.ac.nz

Abstract

Spider: SPecies IDentity and Evolution in R is a new R package implementing a number of useful analyses for DNA barcoding studies and associated research into species delimitation and speciation. Included are functions essential for generating important summary statistics from DNA barcode data, assessing specimen identification efficacy, and for testing and optimizing divergence threshold limits. In terms of investigating evolutionary and taxonomic questions, techniques for assessing diagnostic nucleotides and probability of reciprocal monophyly are also provided. Additionally, a sliding window function offers opportunities to analyse information across a gene, essential for marker design in degraded DNA studies. Spider capitalizes on R’s extensible ethos and offers an integrated platform ideal for the analysis of both nucleotide and morphological data. The program can be obtained from the comprehensive R archive network (CRAN, http://cran.r‐project.org) and from the R‐Forge package development site (http://spider.r‐forge.r‐project.org/).

Number of times cited according to CrossRef: 234

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