Engineering Saccharomyces cerevisiae for next generation ethanol production
Abstract
Conversion of cellulose, hemicellulose or starch to ethanol via a biological route requires enzymatic conversion of these substrates to monosaccharides that can be assimilated by a fermenting organism. Consolidation of these events in a single processing step via a cellulolytic or amylolytic microorganism(s) is a promising approach to low‐cost production of fuels and chemicals. One strategy for developing a microorganism capable of such consolidated bioprocessing (CBP) involves engineering Saccharomyces cerevisiae to expresses a heterologous enzyme system enabling (hemi)cellulose or starch utilization. The fundamental principle behind consolidated bioprocessing as a microbial phenomenon has been established through the successful expression of the major (hemi)cellulolytic and amylolytic activities in S. cerevisiae. Various strains of S. cerevisiae were subsequently enabled to grow on cellobiose, amorphous and crystalline cellulose, xylan and various forms of starch through the combined expression of these activities. Furthermore, host cell engineering and adaptive evolution have yielded strains with higher levels of secreted enzymes and greater resistance to fermentation inhibitors. These breakthroughs bring the application of CBP at commercial scale ever closer. This mini‐review discusses the current status of different aspects related to the engineering of S. cerevisiae for next generation ethanol production. © 2013 Society of Chemical Industry
Number of times cited: 23
- Maria Eugenia Sanz Smachetti, Lara Sanchez Rizza, Camila Denise Coronel, Mauro Do Nascimento and Leonardo Curatti, Microalgal Biomass as an Alternative Source of Sugars for the Production of Bioethanol, Principles and Applications of Fermentation Technology, (351-386), (2018).
- Heinrich Kroukamp, Riaan den Haan, John‐Henry van Zyl and Willem Heber van Zyl, Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae, Biofuels, Bioproducts and Biorefining, 12, 1, (108-124), (2017).
- K. Karimi and Y. Chisti, Bioethanol Production and Technologies, Encyclopedia of Sustainable Technologies, 10.1016/B978-0-12-409548-9.10111-3, (273-284), (2017).
- Fernanda M. Cunha, Alberto C. Badino and Cristiane S. Farinas, Effect of a novel method for in-house cellulase production on 2G ethanol yields, Biocatalysis and Agricultural Biotechnology, 10.1016/j.bcab.2017.01.007, 9, (224-229), (2017).
- Bibekananda Kar, Preeti Verma, Riaan den Haan and Ashwani Kumar Sharma, Characterization of a recombinant thermostable β-glucosidase from Putranjiva roxburghii expressed in Saccharomyces cerevisiae and its use for efficient biomass conversion, Process Biochemistry, 63, (66), (2017).
- Heinrich Kroukamp, Riaan den Haan, Daniël C. la Grange, Ntsako Sibanda, Maria R. Foulquié‐Moreno, Johan M. Thevelein and Willem H. van Zyl, Strain Breeding Enhanced Heterologous Cellobiohydrolase Secretion by Saccharomyces cerevisiae in a Protein Specific Manner, Biotechnology Journal, 12, 10, (2017).
- Teng Zhang and Ming-Jun Zhu, Enhanced bioethanol production by fed-batch simultaneous saccharification and co-fermentation at high solid loading of Fenton reaction and sodium hydroxide sequentially pretreated sugarcane bagasse, Bioresource Technology, 10.1016/j.biortech.2017.01.028, 229, (204-210), (2017).
- Ksenia N. Sorokina, Oxana P. Taran, Tatiana B. Medvedeva, Yuliya V. Samoylova, Alexandr V. Piligaev and Valentin N. Parmon, Cellulose Biorefinery Based on a Combined Catalytic and Biotechnological Approach for Production of 5‐HMF and Ethanol, ChemSusChem, 10, 3, (562-574), (2017).
- Tao Jin, Jieni Lian and Laura R. Jarboe, Ethanol: A Model Biorenewable Fuel, Industrial Biotechnology, (547-572), (2016).
- Leif J. Jönsson and Carlos Martín, Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects, Bioresource Technology, 10.1016/j.biortech.2015.10.009, 199, (103-112), (2016).
- Jhuma Sadhukhan, Jon R. Lloyd, Keith Scott, Giuliano C. Premier, Eileen H. Yu, Tom Curtis and Ian M. Head, A critical review of integration analysis of microbial electrosynthesis (MES) systems with waste biorefineries for the production of biofuel and chemical from reuse of CO 2, Renewable and Sustainable Energy Reviews, 56, (116), (2016).
- David Kennes, Haris Nalakath Abubackar, Marío Diaz, María C Veiga and Christian Kennes, Bioethanol production from biomass: carbohydrate vs syngas fermentation, Journal of Chemical Technology & Biotechnology, 91, 2, (304-317), (2015).
- Ayumi Tanimura, Minako Kikukawa, Shino Yamaguchi, Shigenobu Kishino, Jun Ogawa and Jun Shima, Direct ethanol production from starch using a natural isolate, Scheffersomyces shehatae: Toward consolidated bioprocessing, Scientific Reports, 5, 1, (2015).
- Eric R. Greene, Michael E. Himmel, Gregg T. Beckham and Zhongping Tan, Glycosylation of Cellulases, , 10.1016/bs.accb.2015.08.001, (63-112), (2015).
- Bernardo A. Cinelli, Leda R. Castilho, Denise M.G. Freire and Aline M. Castro, A brief review on the emerging technology of ethanol production by cold hydrolysis of raw starch, Fuel, 10.1016/j.fuel.2015.02.063, 150, (721-729), (2015).
- Rosemary Cripwell, Lorenzo Favaro, Shaunita H. Rose, Marina Basaglia, Lorenzo Cagnin, Sergio Casella and Willem van Zyl, Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast, Applied Energy, 160, (610), (2015).
- Leona Paulova, Petra Patakova, Barbora Branska, Mojmir Rychtera and Karel Melzoch, Lignocellulosic ethanol: Technology design and its impact on process efficiency, Biotechnology Advances, 10.1016/j.biotechadv.2014.12.002, 33, 6, (1091-1107), (2015).
- Ke Zhang, Mengmeng Tong, Kehui Gao, Yanan Di, Pinmei Wang, Chunfang Zhang, Xuechang Wu and Daoqiong Zheng, Genomic reconstruction to improve bioethanol and ergosterol production of industrial yeast Saccharomyces cerevisiae, Journal of Industrial Microbiology & Biotechnology, 42, 2, (207), (2015).
- Lorenzo Favaro, Marko J. Viktor, Shaunita H. Rose, Marinda Viljoen‐Bloom, Willem H. van Zyl, Marina Basaglia, Lorenzo Cagnin and Sergio Casella, Consolidated bioprocessing of starchy substrates into ethanol by industrial Saccharomyces cerevisiae strains secreting fungal amylases, Biotechnology and Bioengineering, 112, 9, (1751-1760), (2015).
- Venkatesh Balan, Current Challenges in Commercially Producing Biofuels from Lignocellulosic Biomass, ISRN Biotechnology, 10.1155/2014/463074, 2014, (1-31), (2014).
- Lorenzo Favaro, Marina Basaglia and Sergio Casella, Innately robust yeast strains isolated from grape marc have a great potential for lignocellulosic ethanol production, Annals of Microbiology, 64, 4, (1807), (2014).
- Chengqiang Wang, Yu Shen, Yanyan Zhang, Fan Suo, Jin Hou and Xiaoming Bao, Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport inSaccharomyces cerevisiae, BioMed Research International, 2013, (1), (2013).
- Marko J Viktor, Shaunita H Rose, Willem H van Zyl and Marinda Viljoen-Bloom, Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases, Biotechnology for Biofuels, 10.1186/1754-6834-6-167, 6, 1, (167), (2013).
