Journal of the Science of Food and Agriculture
Research Article

Modulation of in vitro rumen biohydrogenation by Cistus ladanifer tannins compared with other tannin sources

Mónica Costa

Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal

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Susana P Alves

Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal

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Ângelo Cabo

Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal

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Olinda Guerreiro

Centro de Biotecnologia Agrícola e Agro‐Alimentar do Alentejo (CEBAL), Instituto Politécnico de Beja, P‐7801‐908 Beja, Portugal

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George Stilwell

Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal

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Maria T Dentinho

Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal

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Rui JB Bessa

Corresponding Author

E-mail address: rjbbessa@fmv.ulisboa.pt

Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal

Correspondence to: RJB Bessa, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, P‐1300‐477 Lisboa, Portugal. E‐mail:

rjbbessa@fmv.ulisboa.pt

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First published: 30 April 2016
Citations: 7
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Abstract

BACKGROUND

Tannins are polyphenolic compounds able to modify the ruminal biohydrogenation (BH) of unsaturated fatty acids, but their activity may vary among different tannin sources. The effect of rockrose (Cistus ladanifer) on BH has never been compared with other more common tannin sources. Tannin extracts (100 g kg−1 substrate dry matter) from chestnut (CH), quebracho (QB), grape seed (GS) and rockrose (CL) were incubated in vitro for 6 h with ruminal fluid using as substrate a feed containing 60 g kg−1 of sunflower oil. A control treatment with no added tannins was also included.

RESULTS

Compared with the control, GS and CL, but not CH and QB, increased (P < 0.05) the disappearance of c9,c12‐18:2 with a consequent larger production of c9,t11‐18:2 and t11‐18:1. However, no differences among treatments (P > 0.05) were observed for the disappearance of c9‐18:1 and c9,c12,c15‐18:3. The production of 18:0 was not different (P > 0.05) among treatments, although its proportion in the total BH products was lower (P < 0.05) for GS than for the other treatments.

CONCLUSION

Condensed tannins from GS and, to a lesser extent, from CL stimulate the first steps of BH, without a clear inhibition of 18:0 production. © 2016 Society of Chemical Industry

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