International Journal of Food Science & Technology
Original Article

Aeromonas biofilm on stainless steel: efficiency of commonly used disinfectants

Sara Craveiro

Faculdade de Medicina Veterinária da Universidade de Lisboa, Centro de Investigação Interdisciplinar em Sanidade Animal, Avenida da Universidade Técnica, 1300‐477 Lisboa, Portugal

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Cynthia Alves‐Barroco

Faculdade de Medicina Veterinária da Universidade de Lisboa, Centro de Investigação Interdisciplinar em Sanidade Animal, Avenida da Universidade Técnica, 1300‐477 Lisboa, Portugal

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Maria Teresa Barreto Crespo

ITQB ‐ Instituto de Tecnologia Quí?mica e Biológica, Universidade Nova de Lisboa, Av. da República, 2780‐157 Oeiras, Portugal

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780‐157 Oeiras, Portugal

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António Salvador Barreto

Faculdade de Medicina Veterinária da Universidade de Lisboa, Centro de Investigação Interdisciplinar em Sanidade Animal, Avenida da Universidade Técnica, 1300‐477 Lisboa, Portugal

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Teresa Semedo‐Lemsaddek

Corresponding Author

Faculdade de Medicina Veterinária da Universidade de Lisboa, Centro de Investigação Interdisciplinar em Sanidade Animal, Avenida da Universidade Técnica, 1300‐477 Lisboa, Portugal

Correspondent: Fax: +351‐213652810;

e‐mail: tlemsaddek@fmv.ulisboa.pt or tmsemedo@gmail.com

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First published: 24 January 2015
Cited by: 7
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Summary

Aeromonas spp. are ubiquitous bacteria widely distributed among aquatic environments that have the ability to form biofilms. This aptitude allows them to persist in water distribution systems, contaminating drinking water, food processing surfaces and ultimately food. For this study, the biofilm‐forming ability of aeromonads was evaluated after 48‐h incubation on stainless steel discs at both 4 and 20 °C. Subsequently, disinfectants based on amphoteric surfactants and chlorine compounds were evaluated regarding the capacity to eradicate preformed biofilm and inhibit biofilm formation. Results obtained demonstrated that all strains under analysis were able to form biofilm at both room and refrigeration temperatures. The chlorine‐based disinfectant demonstrated to be efficient in removing preformed biofilm, but both were unsuccessful in preventing biofilm formation, highlighting the importance of adequate cleaning and disinfection procedures, with emphasis on food processing surfaces.

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