CD146‐targeted nuclear medicine imaging in cancer: State of the art

The transmembrane glycoprotein adhesion molecule CD146 is overexpressed in a wide variety of cancers. CD146 expression constitutes a marker of poor prognosis and correlates with cancer progression. Through molecular imaging, a specific biomarker's expression and distribution can be viewed in vivo non‐invasively. Radionuclide‐labeled monoclonal antibodies or relevant fragments that target CD146 may find potential applications in cancer imaging, thereby offering tremendous value in cancer diagnosis, staging, prognosis evaluation, and prediction of drug resistance. This review discusses the recent developments of CD146‐targeted molecular imaging via nuclear medicine, especially in malignant melanoma, brain tumor, lung cancer, liver cancer, breast cancer, and pancreatic cancer. Many studies have proved that CD146 targeting may present a promising strategy for cancer theranostics.

significant morphological changes as epithelial cell adhesion molecules are reduced in expression.As a result, when these cells infiltrate blood vessels, they develop into circulating tumor cells with invasion and migration abilities. 5D146 can also up-regulate the expression levels of CD31 and vascular endothelial growth factor (VEGF), thus promoting tumor angiogenesis and metastasis. 6A high CD146 expression level in different types of cancer is associated with poor prognosis and progression of cancer.5a,7 In recent years, a number of studies have found that CD146 is overexpressed in many types of cancer, including melanoma, breast cancer, liver cancer, lung cancer, pancreatic cancer, prostate cancer, ovarian cancer, and kidney cancer among others.This finding highlights the crucial role of CD146 in cancer development and metastasis.
Molecular imaging technology combines molecular biology with in vivo imaging to reflect the changes in physiological and pathological functions, as well as the expression and distribution of a specific molecule at the molecular, cellular, and organ levels.Molecular imaging based on nuclear medicine is a reproducible, safe, and non-invasive in vivo evaluation technique, which has been widely used for diagnosing, staging, predicting prognosis, and evaluating the efficacy of various cancer treatments 8 .In nuclear medicine, radiotracers are combinations of radioactive atoms and chemicals that target metabolic processes.These radionuclide images are created using positron emission tomography (PET) or single photon emission computed tomography (SPECT), usually in conjunction with a CT scan.ImmunoPET imaging takes advantage of PET's high sensitivity and antibody's high affinity and specificity to achieve non-invasive evaluation of targeted antigens in vivo. 9In the following sections, we will review the applications of CD146-targeted molecular imaging and nuclide therapy using nuclear medicine in common cancers, to find more evidence to confirm the application prospect and value of radionuclide-labeled monoclonal antibodies targeting CD146 or its fragments in tumor imaging.

MOLECULAR MECHANISM OF CD146 IN CANCER
CD146 is necessary for VEGF-induced VEGFR-2 phosphorylation and AKT/p38 mitogen-activated protein kinases (MAPKs) activation, in order to promote endothelial cell migration and the formation of microvessels (Figure 1). 10 CD146 proteins regulate pro-angiogenic genes like interleukin-8 (IL-8), intercellular cell adhesion molecule-1, and matrix metallopeptidase 9 when activated by tumor secretions. 11CD146 binds to Wnt5a activating the c-Jun N-terminal kinase-planar cell polarity (JNK-PCP) pathway and downregulating the expression of β-catenin. 12In addition, by binding directly to Wnt1, CD146 activates fibroblasts via the canonical Wnt/βcatenin pathway.The proliferation of fibroblasts and the production of the extracellular matrix are inextricably linked to this interaction. 13By silencing or deleting CD146, HUVEC proliferation, migration, and tube formation are inhibited, as are VEGFR2, p38, and extracellular regulated protein kinases 1/2 (ERK 1/2). 14The synergistic effect of fibroblast growth factors signaling-dependent ERK activation and CD146-dependent JNK and nuclear factor of activated T cells (NFAT) activation ensure that CD146+ cells simultaneously upregulate AP-1 and NFAT transcription during organ morphogenesis. 15CD146 protein is essential for the E-cadherin-to-N-cadherin switch during transforming growth factor-β (TGF-β) signaling-induced EMT. 16A heterophilic ligand interacts with CD146 to mediate homotypic cell adhesion. 17D146 is overexpressed in many types of cancer, including melanoma, brain tumor, lung cancer, hepatocellular carcinoma, breast cancer, renal cell carcinoma, pancreatic cancer, prostate cancer, gastrointestinal cancer, uterine and adnexal tumors, and other cancer types.Many studies on the mechanism of CD146 in cancer have been carried out.

Melanoma
CD146 is frequently found to overexpress on the surface of metastatic and advanced human melanoma cells. 18In response to VEGF, CD146 deficiency alters the activation of focal adhesion kinase (FAK) and decreases the expression of VEGFR-2/Ve-cadherin. 19 The VEGF-induced pathway and tumor angiogenesis are both impaired in CD146 knockout mice, resulting in the inhibition of VEGF-induced VEGFR-2 phosphorylation and AKT/p38 MAPKs/nuclear factor kappa-B (NF-κB) activation. 20alignant melanoma is promoted by CD146, a novel receptor for S100A8/A9, by activating NF-κB and forming reactive oxygen species. 18Since CD146 is involved in the regulation of inhibitor of DNA binding-1 and activating transcription factor-3 expressions, it, therefore, contributes to the development of melanoma metastases. 21Invasion and metastasis are putatively mediated by laminin-421, which is a primary ligand for CD146. 22By activating the platelet-activating factor receptor (PAFR), proteaseactivated receptor 1 (PAR1) mediates the expression of CD146.In addition to mediating the adhesion of melanoma cells to microvascular endothelial cells, the PAR1-PAFR-MUC18 pathway also modulates transendothelial migration and metastatic retention of melanoma cells. 23 CD146 associated antibodies and signaling pathways.

Brain tumor
There is a high expression of CD146 in glioma stem cells by regulating the cell cycle.Most differentiated cells in the G0/G1 phase are arrested in the cell cycle when CD146 is expressed ectopically in parental glioma cells. 26D146 expression is statistically significantly correlated with lower disease-free survival and overall survival in glioblastomas. 27

Lung cancer
Poor prognosis is associated with CD146 expression in surgically treated non-small-cell lung cancer (NSCLC) 28 .
The expression of EMT markers (i.e., epithelial cadherin, vimentin, and snail) is influenced by CD146, as well as the phosphorylation of AKT.Thus, the migration and prolifer-ation of pulmonary large-cell neuroendocrine carcinoma cells may be regulated by CD146 29 .Stemness and migration of epidermal growth factor receptor-tyrosine kinase inhibitors-resistant (EGFR-TKI-resistant) cells are influenced by CD146.Therefore, CD146 can be a potential target for treating and preventing lung cancers that are resistant to EGFR-TKIs and their subsequent metastases 30 .As a result of CD146-mediated chemosensitivity, mitochondrial 37S ribosomal protein 1/ATP-binding cassette subfamily C member 1 (MRP1/ABCC1) and the PI3K pathway are upregulated through SOX2-dependent signaling 31 .

Hepatocellular carcinoma
CD146 is highly elevated in hepatocellular carcinoma (HCC) cells as a result of positive regulation by Yesassociated protein.The transcriptional and translational activities of c-Jun/c-Fos are initiated by CD146.In addition, eukaryotic initiation factor 4E is bound to c-Jun/c-Fos mRNA through AKT activation, which is promoted by CD146. 32 Patients with basal-like/TNBC and high levels of Notch1, as well as CD146, have poor survival, particularly if they have received chemotherapy 39 .Interestingly, CD146 also acts as a tumor suppressor in breast carcinoma.Breast carcinomas rarely express CD146, but overexpression results in more cohesive cell growth and smaller tumors in nude mice. 40CD146 suppresses breast cancer progression by targeting the CD44downstream signaling pathway that regulates neovascularization and cancer cell motility. 41SM3A regulates neuronilin-1-mediated PTEN-dependent FOXO 3a activation, which stimulates CD146 expression and suppresses breast cancer growth. 42CD146 promotes tamoxifen resistance in breast cancer cells by causing EMT, reducing ER expression, and triggering AKT. 43

Renal cell carcinoma
Clear cell renal cell carcinoma (ccRCC) patients exhibiting CD146 overexpression have poor prognoses.CD146 can be an independent prognostic factor.Among the co-expressed genes of CD146 are those related to the differentiation of Th1 and Th2 cells, differentiation of Th17 cells, and migration of leukocytes across endothelium.Immune infiltration, immunomodulators, and chemokines are strongly correlated with the expression and methylation status of CD146.Furthermore, CD146 expression is linked to the sensitivity and resistance of renal cancer cell lines to certain drugs. 44The expression level of the CD146 gene can serve as an indicator for disease recurrence in cystic RCC (cRCC). 45Compared with other cancers and healthy tissues, the increased expression of CD146 in ccRCC vessels may be related to the induction of VEGF. 46Wnt/β-catenin signaling is activated by insulin-like growth factor binding protein 4, which induces CD146 expression in human RCC cells. 47

Pancreatic cancer
Pancreatic cancer progression is facilitated by a decrease in CD146 expression in cancer-associated fibroblasts (CAFs). 48Pancreatic cancer cells migrate in vitro more readily when they interact with the enhancer-DNA of CD146 and the myeloid ecotropic viral integration site. 49here is a subset of CD34+ cells that express endothelial cell markers, such as CD146, when they differentiate.Angiogenesis-mediated tumor metastasis is further enhanced by the presence of the latter cell type in newly formed vessels. 50

Prostate cancer
Hypermethylation of the CD146 gene is often found in advanced prostate cancer. 51Prostate cancer malignancy is associated with a lack of predominant cytoplasmic membrane expression of CD146. 52A human prostate cancer cell with ectopic expression of CD146 is more likely to metastasize. 53In vivo, increased expression of CD146 stimulates prostate tumorigenesis by promoting proliferation, activating the AKT survival pathway, and increasing prostate cancer's angiogenic ability. 6

Gastrointestinal cancer
Activation of CD146 results in the loss of E-cadherin and the increase in nuclear β-catenin and vimentin expression in mesenchymal cells.A poor prognosis may be associated with CD146 in gastric cancer because it may promote EMT and progression 54 .SRY-box transcription factor 18 (SOX18) triggers gastric cancer metastasis through the activation of CD146 and CCL7 55 .
CD146 expression is correlated with histological grade, Duke's stage, and liver metastasis.It is possible that CD146 is a biomarker for postoperative liver metastases. 56AFs play an important role in colorectal cancer (CRC) progression, and their presence indicates poor prognosis in CRC patients.Pericryptal Lepr-lineage cells proliferate to generate CD146 + CAFs, which act as tumor-promoting immune cells in CRC.Mechanistically, CD146 enhances NF-κB-IL34/CCL8 signaling to promote macrophage chemotaxis when it interacts with interleukin-1 receptor 1. 57 A study has found that cancer stemness and tumorigenesis are promoted by the reduction of CD146 expression in CRC. 58By regulating SPDL1 and CD146, myocardin-related transcription factor B suppresses CRC development. 59

Uterine and adnexal tumors
CD146 is overexpressed in ovarian cancer. 60The presence of CD146 may indicate ovarian carcinomas' malignant potential.Increased signaling in anti-apoptosis, proliferation, survival pathways, and angiogenesis may be associated with ovarian cancer progression. 61In serous ovarian carcinomas and epithelial ovarian cancers, CD146 expression is an independent prognostic factor for survival. 62n ovarian cancer cells, CD146 protects them from apoptosis and promotes their invasion and metastasis by regulating the Rho signaling pathway. 63Through a reduction in proliferation, aerobic glycolysis (metabolism), and angiogenesis, CD146 may suppress in vivo tumorigenesis and malignant progression of ovarian carcinoma cells, likely through the suppression of the PI3K-AKT signaling pathway. 64A positive and significant correlation exists between CD146 expression and the pathological subtype of cervical cancer, as well as the degree of myometrial invasion in endometrial cancer.Cervical and endometrial cancers contain CD146 in the majority of blood vessels, suggesting that CD146 facilitates the metastasis of these cancers through the blood vessels. 65

Other cancer types
As nasopharyngeal carcinoma (NPC) develops, CD146 expression is reduced, but the increased expression is necessary for metastatic growth.This suggests that CD146 can act as both a tumor suppressor like TGF-β as well as a metastasis promoter for NPC. 66A knockdown of CD146 with short hairpin RNA or treatment with an anti-CD146 polyclonal antibody can effectively inhibit neuroblastoma cell growth.They are halted by increased apoptosis via FAK and/or NF-κB signaling. 67CD146 pro-motes osteosarcoma progression by mediating pro-tumoral and angiogenic effects. 68he expression of CD146 is related to many transcription factors.Most mechanism of CD146 in tumor depends on AKT and ERK pathways, which mediate the adhesion of tumor cells to microvascular endothelial cells and promote metastasis.In addition, CD146 is associated with the cell cycle of tumor cells and drug resistance, even though the mechanism is not particularly clear.Interestingly, a dual role of CD146 has been found in some tumors, for example, CD146 acts as both a tumor suppressor like TGF-β and a metastasis promoter in breast cancer and NPC. 66In short, the mechanism of CD146 needs more investigation.

CD146-TARGETED NUCLEAR MEDICINE IMAGING
At present, CD146 targeted imaging is based on immunoPET by using radiolabeled CD146-targeted antibodies, including anti-CD146 monoclonal antibody (mAb), F(ab') 2 fragment, and scFv.Following standard procedures, antibodies are conjugated with appropriate chelating agents and radiolabeled with radionuclides.Serial PET/CT or SPECT/CT imaging is then performed in tumor models. 35CD146 expression has been assessed via imaging in malignant melanoma, brain tumor, lung cancer, hepatocellular carcinoma, and breast cancer among other cancer types (Figure 2).

Breast cancer
Breast cancer accounts for the highest morbidity and the second-highest cancer mortality in women. 72Breast cancer patients with CD146-positive disease have a poor prognosis, especially if they have high-grade, estrogen receptor (ER) negative, progesterone receptor (PR) negative, and/or TNBC. 73Moreover, CD146 is overexpressed in about 63.9% of TNBC patients. 74Patients with primary breast cancer that overexpresses CD146 have significantly reduced overall survival, relapse-free survival, and distant metastasis-free survival. 75Therefore, by evaluating the difference in expression of CD146, breast cancer can be diagnosed, staged, prognosticated, efficaciously evaluated, and drug-resistant evaluated.Ferreira et al. 76 labeled YY146 with manganese-52 ( 52 Mn) radionuclide to construct a radiotracer 52 Mn-DOTA-YY146 (Figure 3A).In the MDA-MB-435 tumor model, the uptake of 52 Mn-DOTA-YY146 increased gradually over time.On the other hand, the uptake was low in MCF-7 tumor due to the low expression of CD146.Li et al. 77 performed immunoPET imaging F I G U R E 2 CD146-targeted imaging via nuclear medicine.(A) 89 Zr-Df-YY146 (left) and 89 Zr-Df-IgG positron emission tomography (PET) (right) imaging enable visualize CD146-expressing A375 xenografts clearly. 69(B) PET images of 64 Cu-NOTA-YY146 in mice containing U87MG (left) and those are preinjected with a blocking dose of unlabeled antibody (right). 27(C) PET Images of tumor-bearing mice derived from lung cancer.H460 (left) and H522 (right) tumors have shown the highest and lowest tracer accumulation, respectively. 70(D) 64 Cu-NOTA-YY146 PET imaging in MDA-MB-435 (left) and pre-blocking (right) tumor models. 35(E) PET images of mice bearing HepG2 and mice pre-injected with a blocking dose of YY146. 71There is a white dashed circle indicating the tumor's location.
after administering 64 Cu-NOTA-YY146 in an orthotopic breast cancer model.Maximum uptake of 14.7 ± 1.75 %ID/g was observed in the MDA-MB-435 tumor model.This value was comparable to the MCF-7 group but was statistically significantly different from the non-specific 64 Cu-NOTA-IgG control groups (p < 0.001).Furthermore, the uptake of 64 Cu-NOTA-YY146 in the MDA-MB-435 lung metastatic tumor group was significantly higher than the MCF-7 tumor group (p < 0.001, Figure 3A).This indicates that the tumor uptake of YY146 is closely related to the expression level of CD146 in orthotopic, subcutaneous, and metastatic breast cancer models.YY146 can also be used as an immunoPET probe to non-invasively assess the distribution and expression of CD146.Thus, CD146-targeted imaging may provide benefits in clinical diagnosis, staging, prognosis prediction, and drug resistance assessment of breast cancer.It may also advance the clinical development of individualized treatment plans.

Malignant melanoma
Malignant melanoma (MM) is a tumor produced by melanocytes and occurs mostly on the skin.CD146 was first found in melanoma cells.While CD146 is considered a marker of MM, it is generally not expressed in benign moles. 79Therapeutic or imaging agents targeting CD146 may have higher specificity, lower toxicity, and better therapeutic efficacy than current conventional treatment modalities.Nollet et al. 1 developed antibodies that recognize the extracellular domain of CD146 and evaluated their ability to identify CD146-expressing cancer cells.These antibodies are known as TsCD146 mAbs (antiCD146 monoclonal antibodies) and Fab'2 fragments from TsCD146 mAb coupled with gallium-68 ( 68 Ga).From the study, PET imaging of nude mice injected with 68 Ga Fab'2-TsCD146 showed that the tumor could be visualized with radiolabeled Fab'2 fragments (Figure 3B).As compared with the IgG control group, the size of the tumor in the treatment group was significantly reduced by 50% after 46 days post-injection.This finding suggests that the antibody fragments not only facilitate immunoPET imaging but also exert some therapeutic effects.It is possible for cancer cells to release microparticles into the bloodstream of patients, and these microparticles contain many proteins from the mother cells.The authors found that this probe could detect CD146-positive tumor particles in the plasma of MM patients.The number of CD146-positive particles was also significantly higher in MM patients, and thus the new antibody could be used to detect CD146-positive tumors through liquid biopsies, a minimally invasive procedure that has gained a lot of attention in the clinical field.Wei et al. 69 64 Cu and 52 Mn in a breast cancer model. 35,76(B) Positron emission tomography (PET) imaging of malignant melanoma mouse model injected with 68 Ga Fab'2-TsCD146 1 .(C) Treatment of hepatocellular carcinoma by intratumoral injection of 125 I-AA98 and subsequent therapy efficacy assessment by 99m Tc-HYNIC-duramycin and 99m Tc-HYNIC-3PRGD2 via SPECT/CT imaging. 78 h, radiotracer uptake was obvious in CD146 overexpressing MM lesions with a statistically significant difference compared to the non-specific imaging agent 89 Zr-DFO-IgG (P < 0.01).This finding demonstrates that PET imaging of 89 Zr-DFO-YY146 can be used to assess the CD146 status in MM.All of the above studies indicate that CD146, as a targeting marker, can greatly improve the clinical management of MM.

Brain tumor
According to the World Health Organization, glioblastoma (GBM) is the most common primary malignancy of the central nervous system and has a median survival time of only 8 months. 80Researchers have recently discovered that CD146 is overexpressed in high-grade gliomas and thus may serve as a viable therapeutic target.Hernandez et al. 81 evaluated CD146 targeting in vivo using immunoPET imaging agent 89 Zr-DFO-YY146 in athymic nude mice bearing subcutaneous U87MG or U251 tumors.In CD146-overexpressing U87MG mice, 89 Zr-DFO-YY146 showed persistent tumor uptake with high specificity.A peak tumor uptake was achieved at 48 h after the imaging agent injection.This result was statistically significantly different from the U251 mouse tumor model that has low CD146 expression (P < 0.05).In CD146 blocking experiments with U87MG-bearing mice, the authors corroborated that 89 Zr-DFO-YY146 is highly specific toward CD146-expressing cells.Their results also validated the accuracy of PET imaging data.The tumor uptake values declined significantly in U87MG-bearing mice given YY146 at 50 mg/kg (P < 0.05).Since macromolecules cannot cross an intact blood-brain barrier (BBB), the effectiveness of antibody-based imaging and treatment in some brain diseases is greatly reduced.However, due to a disrupted BBB in GBM, copper-64 ( 64 Cu) radionuclides coupled with 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA)labeled YY146 ( 64 Cu-NOTA-YY146) could pass through and accumulate persistently in U87MG tumors.A peak tumor uptake was reached at 48 h after injection of the imaging agent.Compared with U87MG tumors, CD146negative U251 tumors showed significantly lower tumor uptake (P < 0.001), while the U87MG tumor uptake value was 37.3 times higher than surrounding normal brain tissue. 27This demonstrates that radiotracer accumulation is highly associated with CD146 expression.Therefore, the expression level of CD146 in GBM can be evaluated noninvasively through immunoPET imaging.

Lung cancer
Lung cancer has the second highest disease incidence (about 12.42%) and the highest mortality rate (about 21.67%) among all cancers. 72More than 78% of patients have local or diffuse metastases at the time of initial diagnosis and most of them have a poor prognosis.28a To increase lung cancer survival rates, it is necessary to diagnose the disease early and treat it promptly.There is an urgent requirement for new approaches for early detection, staging, efficacy monitoring, and prognosis prediction of lung cancer.Several studies have reported that CD146 is closely related to the progression, metastasis, and prognosis of lung cancer. 28,31,82Sun et al. 70 coupled YY146 with NOTA and radiolabeled with 64 Cu.ImmunoPET imaging of 64 Cu-NOTA-YY146 was performed in six subcutaneous xenograft tumor models of lung cancer with differing CD146 expression levels (A549, NCI-H522, NCI-H358, HCC4006, H23, and NCI-H460).The results showed that 64 Cu-NOTA-YY146 has excellent targeting abilities and accumulates rapidly and persistently with high specificity toward CD146-expressing tumors.There was a positive correlation between 64 Cu-NOTA-YY146 tumor uptake and CD146 expression levels (r 2 = 0.98, p < 0.01), with the highest uptake value of 20.1 ± 2.86 %ID/g in the H460 model that has an overexpression of CD146 proteins.On the other hand, only 4.10 ± 0.98 %ID/g was found in the H522 model, which has a low CD146 expression.Further development of 64 Cu-NOTA-YY146 may allow for improved stratification of lung cancer patients and enhanced monitoring of therapeutic response.Malignant cells are known to acquire metastatic potential and apoptotic resistance through EMT.Upon overexpression of CD146, epithelial markers are downregulated and mesenchymal markers are upregulated.CD146 has also been recognized as an ideal biomarker for lung cancer.By first performing in vitro assays, YY146 was determined to be well suited for CD146 immunoPET imaging in two models of intrapulmonary metastasis (H460 and H358) of NSCLC.Intrapulmonary metastases in the H460 model showed rapid, sustained, and specific radiotracer uptake while reaching a peak uptake value 48 h after agent injection.The uptake was approximately 13.85 ± 1.07 %ID/g (n = 4), which was statistically significantly different from the control H358 model that exhibits a low CD146 expression (6.08 ± 0.73 %ID/g, p < 0.05) 83 .The above studies suggest that CD146-targeted immunoPET imaging not only non-invasively evaluates the differential expression of CD146 in primary lung cancer but also enables the early detection of intrapulmonary metastases.

Hepatocellular carcinoma
The most common primary malignant tumor of the liver in adults is HCC.HCC ranks as the fifth leading cause of cancer death among men (about 6.34%) and seventh among women (about 3.52%). 84Metastasis and recurrence of HCC are the main causes of reduced patient survival, and around half of the patients diagnosed with advanced HCC cannot be resected or transplanted. 85Previous research has shown that the expression of CD146 is significantly increased in liver cancer tissues and is an indicator of disease development, metastasis, and poor prognosis. 86D146 is a novel tumor marker for HCC and acts as a potential target for the diagnosis and treatment of HCC. 32ernandez et al. 71 constructed a radiotracer ( 89 Zr-DFO-YY146) by conjugating 89 Zr radionuclide to YY146 via the chelating agent DFO.After the administration of 89 Zr-DFO-YY146, a statistically significant difference was found among the CD146-overexpressing HepG2 subcutaneous graft tumor model, Huh7 tumor group, and HepG2 blocking or low CD146 expression group (P < 0.001).Therefore, CD146 is a suitable target for noninvasive diagnosis and evaluation of HCC.
AA98 mAb is a promising mAb that acts against CD146 and has inhibitory effects on angiogenesis and tumor growth.Zhou et al. 78 labeled AA98 with iodine-125 ( 125 I) and injected it intratumorally to treat HCC xenograft lesions.To evaluate the treatment efficacy of this radioligand, apoptosis-targeted imaging with 99m Tc-HYNICduramycin and angiogenesis-targeted imaging with 99m Tc-HYNIC-3PRGD2 were employed (Figure 3C).The high dose group (200 μCi 125 I-AA98 mAb) demonstrated the highest relative inhibitory rates of tumor (69.5%) and apoptotic index (11.8± 3.8%), indicating the potential of 125 I-AA98 mAb to treat CD146-expressing HCC.In conclusion, CD146-targeted molecular imaging and therapy may bring tremendous value in the detection, prognosis prediction, and management of hepatic malignancies.

Other cancer types
This deadly disease occurs when the mesothelium, a protective layer surrounding most of the body's internal organs, undergoes a malignant transformation.Epithelioid mesothelioma, sarcomatoid mesothelioma, and mixed mesothelioma are the most common types.Studies of mesothelioma tissue microarrays revealed that CD146 is overexpressed in more than 80% of epithelioid and sarcomatous mesotheliomas, but not in normal mesothelium. 87n et al. 88 selected several human scFvs from phage antibody libraries that are highly binding to the surface epitopes on mesothelioma cells that are clinically related to mesothelioma.They used technetium-99m ( 99m Tc)-labeled anti-CD146 scFvs to detect tumor cells in mesothelioma organ xenografts in vivo using SPECT/CT.It was found that anti-CD146 M1 scFv accumulates more readily in mesothelioma xenografts than the control scFv at a higher level than surrounding soft tissues, thus demonstrating the potential utility of using noninvasive imaging and immunotherapy to treat specific diseases.1).To be consistent with the half-life of the whole antibody, most studies selected nuclides with relatively long half-lives, such as 52 Mn, 64 Cu, and 89 Zr.However, some studies used 68 Ga, which should be proper for Fab'2 fragments with a shorter half-life in the blood.Generally, the nuclide with a short half-life is more suitable for clinical use.Therefore, future research could tend to focus on antibody fragments using short half-life nuclides, whereas there are few studies about radionuclide therapy.

CONCLUDING REMARKS AND FUTURE DIRECTIONS
CD146 is overexpressed in a variety of malignancies and is closely associated with tumor aggressiveness, metastasis, poor prognosis, and drug resistance.CD146, therefore, is highly attractive for imaging and therapy of cancer.CD146-targeted mAbs or their fragments can be utilized in cancer immunoimaging to aid in the diagnosis, staging, and prognosis prediction of tumors, as well as provide a novel clinical imaging approach.CD146 is also a predictive marker of tumor angiogenesis.Consequently, CD146-targeted imaging has been explored to monitor the progression of angiogenesis reperfusion in vivo and track the recovery in mouse hindlimb ischemia models. 89ome studies have used relatively long half-life nuclides to label antibodies targeted by CD146, including 89 Zr, 64 Cu, and 52 Mn, which is consistent with the circulating time of antibodies in vivo.However, for clinical applications, short half-life nuclides and faster-circulating antibody fragments seem to be more popular and promising.A study has labeled the Fab'2 antibody fragment targeting CD146 with a shorter half-life nuclide 68 Ga.Although the anti-body fragment still has the defect of insufficient tumor accumulation, this suggests that we should make more improvements in the future.
In terms of cancer theranostic applications, YY146 labeled with therapeutic radionuclides, for example, lutetium-177 ( 177 Lu), may represent a novel treatment for patients who have developed tolerance toward conventional therapies.Our research group has used 177 Lu-labeled anti-Trop2 and CD38-targeting monoclonal antibodies to treat pancreatic cancer and lymphoma and confirmed that 177 Lu-labeled antibodies have good efficacy in xenograft tumor models.Therefore, it is likely that 177 Lu-labeled CD146-targeted antibody will also have good prospects but further studies should be performed. 90Antibody-based imaging agents facilitate higher tumor uptake owing to their slow pharmacokinetics and long circulation time in vivo.On the other hand, the fast blood clearance of radiolabeled antibody fragments provides a better signal-to-noise ratio, resulting in higher-quality images.The coupling of short half-life nucleophiles (e.g., 68 Ga) necessitates same-day immunoPET imaging, which may increase the likelihood of clinical implementation.Although current studies have been limited to small animal experiments, CD146 targeting proves to be a feasible strategy for cancer theranostics and holds great promise in furthering personalized precision medicine.

F I G U R E 3
Theranostic application of three different molecules targeting CD146.(A) Imaging of YY146 mAb labeled with This work was supported by the National Natural Science Foundation of China (82171970 and 81871385), the Beijing Science Foundation for Distinguished Young Scholars (JQ21025), the Beijing Municipal Science & Technology Commission (Z221100007422027), the Peking University Medicine Fund of Fostering Young Scholars' Scientific & Technological Innovation (BMU2022PY006), the University of Wisconsin -Madison, and the National Institutes of Health (P30 CA014520 and T32 CA009206).

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O N F L I C T O F I N T E R E S T S TAT E M E N T Weibo Cai declares conflict of interest with the following corporations: Actithera, Inc., Rad Source Technologies, Inc., Portrai, Inc., rTR Technovation Corporation, and Four Health Global Pharmaceuticals Inc.All other authors declare no conflict of interest.O R C I D Jessica C. Hsu https://orcid.org/0000-0002-3599-2834Lei Kang https://orcid.org/0000-0001-8729-4547R E F E R E N C E S 87 Different nuclear medicine tracers targeting CD146.
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