Predictors of mTICI 2c/3 over 2b in patients successfully recanalized with mechanical thrombectomy

Abstract Objective For patients presenting with acute ischemic stroke (AIS) caused by large vessel occlusions (LVO), mechanical thrombectomy (MT) is the treatment standard of care in eligible patients. Modified Thrombolysis in Cerebral Infarction (mTICI) grades of 2b, 2c, and 3 are all considered successful reperfusion; however, recent studies have shown achieving mTICI 2c/3 leads to better outcomes than mTICI 2b. This study aims to investigate whether any baseline preprocedural or periprocedural parameters are predictive of achieving mTICI 2c/3 in successfully recanalized LVO patients. Methods We conducted a retrospective multicenter cohort study of consecutive patients presenting with AIS caused by a LVO from 1 January 2017 to 1 January 2023. Baseline and procedural data were collected through chart review. Univariate and multivariate analysis were applied to determine significant predictors of mTICI 2c/3. Results A total of 216 patients were included in the study, with 159 (73.6%) achieving mTICI 2c/3 recanalization and 57 (26.4%) achieving mTICI 2b recanalization. We found that a higher groin puncture to first pass time (OR = 0.976, 95%CI: 0.960–0.992, p = 0.004), a higher first pass to recanalization time (OR = 0.985, 95%CI: 0.972–0.998, p = 0.029), a higher admission NIHSS (OR = 0.949, 95%CI: 0.904–0.995, p = 0.031), and a lower age (OR = 1.032, 95%CI: 1.01–1.055, p = 0.005) were associated with a decreased probability of achieving mTICI 2c/3. Interpretation A lower groin puncture to first pass time, a lower first pass to recanalization time, a lower admission NIHSS, and a higher age were independent predictors of mTICI 2c/3 recanalization.


Introduction
Acute ischemic stroke (AIS) is a key cause of mortality and morbidity worldwide. 1Up to 30% of AIS cases are caused by anterior circulation large vessel occlusions (LVOs), which have been shown to cause disproportionately worse outcomes than other sites of occlusion. 2,3For these patients, mechanical thrombectomy (MT) has become the standard of care for up to 24 hours in patients deemed eligible. 4,5he extent of reperfusion achieved after MT is typically assessed with the modified Thrombolysis in Cerebral Infarction (mTICI) scale.7][8][9] The superior outcomes associated with mTICI 2c/3 underscores the importance of investigating prognostic biomarkers predic tive for excellent recanalization.
The objective of the current study is to therefore investigate which baseline preprocedural or periprocedural parameters are predictive of excellent recanalization (defined as mTICI 2c/3) in successfully recanalized LVO patients (defined as mTICI 2b/2c/3).

Materials and Methods
The corresponding author had full access to all the data in the study and takes responsibility for its integrity and the data analysis.

Study population
We performed a retrospective multicenter cohort study of all patients presenting with AIS caused by a large vessel occlusion from 1 January 2017 to 1 January 2023.This study was approved by our institutional review board (protocol code JHU-IRB00269637) and complies with the Health Insurance Portability and Accountability Act.Inclusion criteria were patients with AIS secondary to anterior circulation LVO (defined as distal intracranial ICA, M1, and proximal M2 segments of the middle cerebral artery (MCA)) who were evaluated within 24 h of symptom onset and successfully treated with MT to achieve mTICI 2b, 2c, or 3 recanalization.The mTICI was determined by the performing neurointerventionalist.Patients with intracranial hemorrhage on baseline imaging were excluded from the study.Study participants were then categorized into either a mTICI 2b or a combined mTICI 2c/3 cohort for comparative analysis.

Data collection
Baseline, clinical, and time parameter data were abstracted from review of electronic medical records.Baseline labs and vitals were based on first available values on initial admission.For further details, refer to our preliminary paper which used the same methodology. 10ASPECTS score was assessed by an experienced neuroradiologist (Dr.Vivek Yedavalli, 6 years of experience).

Study outcomes
The primary outcome measure was achieving mTICI 2c/3 recanalization.

Statistics
The data were managed and analyzed using IBM SPSS statistics (Statistical Package for Social Sciences) software version 26, IBM Corp., Chicago, IL, USA, 2013 and Microsoft Excel.All qualitative data were described as n, %, and compared using chi-squared and Fisher's exact tests.All quantitative data except for time parameter data were reported as mean AE SD (standard deviation) and compared using independent t-tests.Time parameter data were reported as median (IQR) with p-value determined by a nonparametric test.Each baseline variable was independently assessed through univariate analysis.Those variables with a p-value < 0.1, as well as age and sex, were then entered in a logistic regression model (backward stepwise method) to determine the independent factors associated with mTICI 2c/3.p-values of < 0.05 were considered significant.
The baseline characteristics of patients who achieved mTICI 2c/3 were compared with those who achieved mTICI 2b (Table 1).The mean AE SD age of the studied cases was 68.5 AE 15.1 years.Notably, patients who achieved mTICI 2c/3 were more likely to have a lower admission NIHSS score than those who achieved mTICI 2b, which trended toward significance (14.7 AE 6.7 vs. 16.8AE 7.6, p = 0.053).

Discussion
In this multicenter, retrospective study, we found that a lower groin puncture to first pass time, a lower first pass to recanalization time, a lower admission NIHSS, and a higher age were independent predictors of excellent recanalization.
MT is the treatment standard of care in many patients presenting with AIS secondary to LVOs.7][8][9] Which factors determine whether successfully recanalized patients achieve excellent recanalization is still underexplored.Our study aimed to fill this knowledge gap by examining potential prognostic biomarkers predictive of excellent recanalization following endovascular treatment.
In our study, a lower groin puncture to first pass time was an independent predictor of excellent recanalization.The mTICI 2c/3 group had a median groin puncture to first pass time of 22.0 [16.0-29.0]min as compared to the mTICI 2b group average of 27.0 [18.0-38.5]min, representing a roughly 5-min difference between the two cohorts.Thus, minimizing the time needed to deploy the clot retrieval device after groin puncture is of key importance, regardless of the type of device used.While a previous study showed that shorter MT procedure times are  11 this is the first study to our knowledge that specifically identified groin puncture to first pass time as being associated with excellent recanalization.Initiating MT through a groin puncture is an invasive procedure that may destabilize the patient, and prolonging the time needed until device deployment can make this deployment less effective.3][14] Delays after groin puncture gives more time for this neuroinflammation to occur, which may in turn hinder both the guiding of the catheter and the deployment of the clot retrieval device, reducing the extent of reperfusion achieved.Minimizing the time needed to perform the procedure is also overall advantageous, as faster recanalization may improve the quality of reperfusion, such as by reducing ischemic damage or decreasing the likelihood of distal clot embolization. 10,11,15,16longside a shorter groin puncture to first pass time, our study found that a lower first pass to recanalization time was also an independent predictor of excellent recanalization.The mTICI 2c/3 group had a median first pass to recanalization time of 5.0 [2.0-18.0]min as compared to the mTICI 2b group average of 14.0 [3.0-38.5]min, representing a nearly 10-min difference between the two cohorts.Importantly, the number of passes needed to achieve recanalization was not significantly different between the two study cohorts (p = 0.274).This suggests that to achieve excellent recanalization, it is important to minimize delays in retrieving the clot after the clot retrieval device is deployed.1][22] Indeed, many proceduralists wait for up to several minutes following stent deployment before beginning the clot removal process. 23,24While there may be a benefit to allocate some amount of waiting time when using stent retrievers to improve clot retrieval, our study shows that excessive waiting will ultimately lead to worse recanalization regardless of the thrombectomy device used.Future studies should clarify the optimal balance between maximizing device-clot integration and minimizing unnecessary delays of clot removal to achieve maximum recanalization.Lower admission NIHSS was also an independent predictor of mTICI 2c/3 recanalization.6][27][28] There is also some evidence that lower admission NIHSS is associated with greater mTICI score following MT. 6,29However, this is the first study to our knowledge that shows that a lower admission NIHSS can act as an independent predictor of mTICI 2c/3 over 2b within the subset of successfully recanalized patients.There are several possible reasons for this.First, patients who present with a more severe stroke may have a larger and/or more invasive blood clot that is more challenging to remove, resulting in less complete recanalization of the occluded vessel (s). 30,31Second, higher admission NIHSS is associated with worse collateral status, [32][33][34] which in turn is associated with less successful reperfusion following endovascular treatment. 35,36Third, patients with more severe stroke symptoms are more likely to already have extensive and/ or irreversible ischemic brain damage than those with milder symptoms, whether due to the severity of the clot or a delay in their evaluation, limiting the amount of reperfusion that can be achieved through MT.
Surprisingly, our study also found that older age was an independent predictor of excellent recanalization.2][43] The relationship between age and post-MT radiographic outcomes like mTICI, however, has been much less studied.One possible explanation for why older age may increase mTICI 2c/3 recanalization over mTCI 2b is treatment selection bias.Due to concerns of performing an invasive procedure like MT in elderly patients, the treatment team may only select older patients with favorable clinical and radiographic characteristics, like low clot burden and high collateral status 44 ; these patients will be much more likely to achieve excellent recanalization.In contrast, nearly all younger patients will be considered a candidate for mechanical thrombectomy, even those with unfavorable characteristics that reduce the likelihood of achieving excellent recanalization.With an increasing older adult population, further research on the impact of age on stroke treatment outcomes is needed.
There are several study design limitations that should be considered when interpreting these results.First, our study has the inherent limitations of a retrospective approach.We aimed to minimize this by only including consecutive patients from two comprehensive stroke centers within our larger hospital enterprise.Second, the mTICI scores were self-adjudicated by the operating physician, and thus may not be completely standardized.However, all mTICI scores used in the study were adjudicated by experienced neurointerventionists with years of training, all within the same institution.Third, our sample size was imbalanced between the 2c/3 and 2b patient cohorts (73.6% of the total study population vs. 26.4%,respectively), which is a potential source of bias.Prior studies have shown that more patients generally achieve mTICI 2c or 3 than 2b following MT. 6,8As an example, in a 2017 study that included 1933 successfully recanalized patients, 63.8% of the total study population was in the 2c/3 cohort. 6Another 2021 study that included 149 successfully recanalized patients had 81.2% of their study population in the 2c/3 cohort. 8Our sample size differences therefore lie within the ranges of prior investigations.Fourth, identifying periprocedural factors that are independent predictors of excellent recanalization (i.e., groin puncture to first pass time) may be less useful for selecting patients for MT compared to identifying predictive baseline characteristics (i.e., age or NIHSS score), as the former is only determined after the procedure has started.However, they are still useful for maximizing outcomes after the patient has been selected for MT and as quality improvement markers for the procedure itself.
In conclusion, we report that lower groin puncture to first pass time, lower first pass to recanalization time, a lower admission NIHSS, and older age were independent predictors of mTICI 2c/3 recanalization.Understanding these findings may help providers better maximize the likelihood of achieving excellent recanalization in patients presenting with LVOs.Additional prospective studies must be conducted to further elucidate the impact of these parameters on achieving excellent recanalization.

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2023 The Authors.Annals of Clinical and Translational Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Table 1 .
Descriptive statistics among studied cases and comparison according to mTICI grade.Data presented as Mean AE SD unless mentioned otherwise.BMI, body mass index; BUN, blood urea nitrogen; DBP, diastolic blood pressure; HR, heart rate; RR, respiratory rate; NIHSS, National Institutes of Health Stroke Scale; SBP, systolic blood pressure.
*Significant (<0.05).ª 2023 The Authors.Annals of Clinical and Translational Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.

Table 2 .
Comparison of intervention and time parameter data between the mTICI 2b and mTICI 2c/3 cohorts.

Table 3 .
Multivariate logistic regression of studied variables in predicting mTICI 2c/3 in all patients.