Lonely in the Dark: Trauma Memory and Sex‐Specific Dysregulation of Amygdala Reactivity to Fear Signals

Abstract Loneliness exacerbates psychological distress and increases the risk of psychopathology after trauma exposure. However, it is still unclear whether a lack of social connectedness affects trauma‐related intrusions and the neural processing of fear signals. Moreover, it is uncertain, whether loneliness plays a different role in women and men. A prestratification strategy is used and n = 47 (n = 20 women) healthy lonely individuals and n = 35 controls (n = 18 women) are recruited. Participants are exposed to an experimental trauma and evoked intrusive thoughts in daily life are monitored for three consecutive days. Functional magnetic resonance imaging is used to assess neural habituation to fearful faces and fear learning (conditioning and extinction) prior to trauma exposure. The results reveal a significant interaction between loneliness and sex such that loneliness is associated with more intrusions in men, but not in women. A similar pattern emerges at the neural level, with both reduced amygdala habituation to repeated fearful faces and amygdala hyperreactivity during the conditioning of fear signals in lonely men. The findings indicate that loneliness may confer vulnerability to intrusive memories after trauma exposure in healthy men and that this phenotype relates to altered limbic processing of fear signals.


Introduction
Loneliness, defined as the discrepancy between desired and actual social connectedness, [1] is a growing problem in modern DOI: 10.1002/advs.202105336 societies. [2] Loneliness can be considered as the social equivalent to hunger or pain to meet social needs and has been associated with increased mortality, resembling risk factors like obesity or smoking. [3,4] Furthermore, loneliness is closely linked with various psychiatric disorders such as substance abuse, depression, and anxiety disorders. [5,6] Importantly, loneliness also constitutes a risk factor for developing posttraumatic stress disorder (PTSD) following a traumatic experience. [7,8] In fact, loneliness predicts future PTSD and is predicted by past PTSD symptoms, indicating a bidirectional relationship between PTSD and social connectedness. [9,10] PTSD is a debilitating and frequently chronic condition characterized by intrusive thoughts about the traumatic experience as a key symptom. [11][12][13] Intrusions are defined as involuntarily spontaneous memories of the distressing incident, mainly experienced as visual forms of mental imagery. [14][15][16] The lifetime prevalence of PTSD varies substantially between sexes, with women being twice as likely to develop PTSD than men. [17] Current neurocircuit models of PTSD highlight dysfunction of the amygdalahippocampus complex as a core mechanism underlying the persistence of intrusive memories. Modern trauma-focused psychotherapies for treating intrusions and other PTSD symptoms often include an exposure-based intervention to reduce fear responses. [18] Mechanistically, this decrease in fear responses can be achieved by both fear extinction and fear habituation. The former is characterized by a progressive decrement of a conditioned fear response (CR) when a conditioned stimulus (CS) is repeatedly presented in the absence of an aversive unconditioned stimulus (UCS) with which it has previously been paired, while the latter is based on repeated exposure to the (imagined) UCS. In fact, both fear extinction and habituation recruit overlapping forebrain structures including the amygdala. [19] The experimental trauma paradigm is a widely used and reliable method to evoke intrusions by showing traumatizing film footage in a controlled laboratory setting. [14,20,21] On a neural level, increased reactivity in the amygdala, hippocampus, insula, and anterior cingulate cortex during trauma exposure predicts increased intrusive thoughts. [22,23] Interestingly, neural processing during fear extinction has also been linked to intrusion frequency in an experimental trauma paradigm and reduced extinction capacity predicts PTSD development. [24,25] Furthermore, women reported more intrusive symptoms following the trauma paradigm than men, and this sex difference was related to peritraumatic responding and slowed extinction learning in women. [26] Likewise, women showed a sustained amygdala response to negative evocative images relative to men. [27] We previously found that strong trauma disclosure reduces intrusions and alters amygdala functional connectivity following trauma exposure only in individuals with heightened concentrations of the hypothalamic peptide oxytocin after intranasal administration. [28] Given a crucial role of oxytocin in safety learning and a reduced oxytocin reactivity to positive social interactions in people experiencing loneliness, [29,30] this raises the intriguing possibility that loneliness influences intrusions after trauma exposure by modulating self-disclosure and amygdalarelated fear processing. Furthermore, a recent large-scale study indicated a higher prevalence of loneliness in men than in women, [31] and a growing number of studies reported sexspecific effects of loneliness. For instance, loneliness was associated with more pronounced within-network coupling of the default network in men than in women, and brain volume effects in the limbic system were linked to the frequency and intensity of social contact in a sex-dependent manner. [32][33][34] Surprisingly, however, the impact of loneliness on fear conditioning/extinction and fear habituation as well as the possible moderation by sex remain unclear. Therefore, this study aimed to examine lonelinessassociated neurobiological risk factors for intrusive thoughts in an experimental prospective study design.
To this end, we recruited a prestratified sample of 82 healthy volunteers assigned to either a high-lonely and low-lonely group to test how loneliness interacts with sex to influence the neural processing of fear signals and the formation of intrusive thoughts. During functional magnetic resonance imaging (fMRI), subjects completed an emotional face-matching task to assess neural responses to fearful faces and the habituation of these responses. In addition, we used a classical Pavlovian fear conditioning and extinction paradigm with two social and nonsocial stimuli one of each paired (CS+) and one without (CS−) an electric shock. To explore hormonal group differences, blood samples were taken before the fMRI session. Subsequently, we probed psychological (dissociative symptoms, state anxiety, positive and negative affect), physiological (electrodermal activity, pupil sizes), and hormonal (oxytocin) stress responses during an experimental trauma paradigm. The trauma paradigm consisted of a 24-min-long aversive video to mimic trauma exposure. Furthermore, evoked intrusions and communication behavior were monitored via online diaries during three consecutive days after trauma exposure. The total number of intrusions, trauma disclosure (i.e., desire to talk and talk duration), intrusion stress ratings and the level of amygdala reactivity in neural fear processing served as primary study outcomes. We hypothesized that lonely individuals would exhibit more pronounced responses to the experimental trauma film and experience more intrusions. Furthermore, we expected to observe loneliness-dependent hyperreactivity to fearful faces and fear-conditioned stimuli in the amygdala, as well as changes in functional connectivity in a network responsible for fear processing. [35][36][37] Given previous findings about sex differences in the effects of loneliness and the formation of intrusive memories, we explored sex as a moderator variable.

Subclinical Psychiatric Symptoms, Loneliness, and Sex Differences
Psychiatric symptoms were measured via questionnaires during a screening interview. In addition, blood samples were taken before fMRI scanning. High-lonely subjects reported more depressive symptoms, alexithymia, childhood maltreatment, social interaction anxiety, and subjective stress compared to low-lonely participants (all ps < 0.02; shown in Table S7, Supporting Information). Furthermore, high-lonely participants had smaller and less diverse social networks and received less social support (all ps < 0.03). In addition, across groups, women reported having more social support than men (F (1,78) = 5.12, p = 0.03, p 2 = 0.06). There were no significant interactions between sex and loneliness in psychiatric symptoms and social network quality (all ps > 0.05). Besides the expected sex differences, we found a significant sex*loneliness interaction in estradiol levels (F (1,65) = 7.60, p = 0.01, p 2 = 0.11), showing that high-lonely women exhibited higher estradiol levels than low-lonely women at the fMRI session (t (16.55) = 2.62, p cor = 0.04, d = 0.87; shown in Table S1, Supporting Information). For a detailed list of differences in psychiatric symptoms between groups see Table 1.

Psychological and Physiological Reaction to the Trauma Video
An experimental trauma paradigm was conducted after the fMRI. Dissociative symptoms, positive and negative affect, state anxiety, and saliva oxytocin were measured before and after trauma exposure via questionnaires and saliva samples. Physiological stress markers (pupil size and electrodermal activity) were measured during trauma exposure. After trauma exposure, subjects showed dissociative symptoms (mean ± SD = 1.24 ± 1.18, one-sample ttest against zero: t (77) = 9.36, p < 0.01, d = 1.06) and reported high arousal (76.87 ± 23.53) induced by and low valence (9.35 ± www.advancedsciencenews.com www.advancedscience.com   Figure 1F). Thus, the trauma video elicited a psychological and physiological stress response regardless of sex and loneliness.

Intrusive Thoughts
The primary study outcomes, intrusive thoughts, trauma disclosure (desire to talk, talk duration), and intrusion stress ratings were measured via online questionnaires on three consecutive days after trauma exposure. Across loneliness groups, women ex-perienced more intrusions than men (main effect of sex: F (1,77) = 8.53, p = 0.01, p 2 = 0.10). However, our results revealed a significant interaction between loneliness and sex (F (1,77) = 5.57, p = 0.02, p 2 = 0.07), such that loneliness was associated with more intrusive memories in men but fewer intrusions in women (shown in Figure 2A). Post hoc t-tests further revealed that lowlonely women exhibited significantly more intrusions than lowlonely men (t (33) = 3.97, p cor < 0.01, d = 1.39), while there was no significant sex difference in high-lonely individuals (t (44) = 0.39, p = 0.70, d = 0.12). Furthermore, analysis of the desire to talk about the trauma movie yielded a pattern consistent with intrusion effects (F (1,65) = 5.62, p = 0.02, p 2 = 0.08; shown in Figure 2B). High-lonely woman showed a decreased desire, whereas high-lonely men exhibited an increased desire in contrast to lowlonely individuals. Again, post hoc t-tests revealed that low-lonely women showed an increased desire to talk in contrast to lowlonely men (t (32) = 2.66, p cor = 0.046, d = 0.91). In addition, highlonely subjects talked less about the movie (main effect of loneliness: F (1,49) = 9.85, p < 0.01, p 2 = 0.17; shown in Figure 2C), indicating that the sex-specific association of loneliness with the desire to talk about the traumatic experience did not lead to a similar pattern in actual trauma disclosure. Neither sex nor loneliness significantly affected intrusion stress ratings (all ps > 0.05).

Emotional Face-Matching: fMRI Effects
fMRI scanning was conducted before trauma exposure and consisted of an emotional face-matching task and a fear conditioning and extinction paradigm. In the emotional face-matching task, participants had to match two simultaneously presented pictures at the bottom with a target picture presented at the top of the screen. In the fear conditioning and extinction paradigm, participants had to press a button before the UCS to indicate if they believed that they would receive an electric impulse. Responses were acquired with an fMRI compatible response grip system to measure reaction times and contingency ratings. Amygdala reactivity in both paradigms served as primary study outcome. There was no significant interaction effect of sex and loneliness on the neural response to fearful faces per se, but amygdala habituation was characterized by sex*loneliness interactions. Habituation to fearful faces in the right amygdala was reduced in high-lonely men compared to high-lonely women, while this pattern was   Figure 3B) in contrast to high-lonely women, while this pattern was reversed in low-lonely individuals. Collectively, amygdala habituation and functional connectivity in high-lonely men seemed to be most pronounced in response to fearful stimuli, whereas amygdala habituation in high-lonely women seemed to be altered regardless of the emotional valence of the social stimuli. Further behavioral and neural results of the emotional face matching task are reported in in Tables S2 and S3 of the Supporting Information.

Fear Conditioning and Extinction: Contingency Ratings
Successful conditioning was evident in higher contingency ratings of the CS+ compared to the CS− in the second half of the COND (conditioning) task (interaction effect of time (first half, second half) and condition (CS+, CS-): F (1,64) = 54.79, p < 0.01, p 2 = 0.46). Likewise, a significant time*condition interaction (F (1,63) = 49.23, p < 0.01, p 2 = 0.44) for the contingency ratings showed reduced shock expectations in the course of the EXT (extinction) task (shown in Table S5, Supporting Information). In addition, a time*condition interaction with sex and loneliness was evident such that high-lonely men showed higher contingency ratings (i.e., expected more electric shocks) to the CS+ in the second half of the COND phase than high-lonely women (time*condition*sex*loneliness; F (1,64) = 5.41, p = 0.02, p 2 = 0.08). There were no significant interactions with the factor sociality or sex * loneliness interactions in the EXT phase.

Fear Conditioning and Extinction: fMRI Effects
In the COND phase, the CS+ elicited activations in a fear conditioning network [35] including the amygdala (COND CS+ > CS− MNI coordinates and statistics are listed in Table S5, Supporting Information). Importantly, amygdala reactivity to fear signals in the early phase of COND compared to that in EXT was associated with loneliness in a sex-specific manner (sex*loneliness interactions: High-lonely men exhibited higher amygdala activation than highlonely women, while this effect was reversed in low-lonely individuals. We also observed a loneliness*sex interaction in the functional connectivity of the amygdala during fear conditioning/extinction. High-lonely men exhibited a stronger coupling between the left amygdala and orbitofrontal cortex (MNI xyz : −44, 28, −16, k = 98, p FWE = 0.02; COND CS+ > CS− > EXT CS+ > CS− ; shown in Figure 4B) compared to high-lonely women during the conditioning of fear signals and this pattern was reversed for low-lonely individuals.
Importantly, including psychiatric symptoms that differed between groups (shown in Table 1; Table S7, Supporting Information), social support, hormonal contraception, and estradiol levels as covariates did not change the significant sex*loneliness interactions observed for intrusions and parameter estimates of significant clusters (see the Supporting Information). Taken together, these findings indicate that fear habituation and conditioning mechanisms in an amygdala network vary as a function of loneliness and sex.

Discussion
The present study aimed to probe loneliness as a risk factor for increased physiological and psychological responses to an experimental trauma film. We further examined whether loneliness effects were moderated by sex and related to changes in the neu-ral processing of fear signals. Our results revealed a significant interaction between sex and loneliness in intrusive thought formation such that loneliness was positively associated with more intrusions in men, but not women. A similar pattern emerged at the neural level, with both reduced amygdala habituation to repeated fearful faces and amygdala hyperreactivity during the conditioning of fear signals in high lonely men, but not in women. Our findings indicate that loneliness is indeed a risk factor for increased intrusions after trauma exposure in high-lonely men, and this relates to amygdala reactivity in a network responsible for fear conditioning and habituation in these vulnerable individuals. The experimental trauma paradigm elicited marked stress responses evident in significant psychological and physiological changes across all subjects. We did not observe significant loneliness or sex effects on these acute responses after the trauma film, indicating that loneliness may be more important in long-term coping with the traumatic experience. As expected, high-lonely subjects talked less about their traumatic experience than lowlonely individuals. Disclosure of emotional and traumatic events is known to reduce distress and may promote extinction of fear-related memories. [38,39] Furthermore, discussing traumatic memories reduces PTSD symptoms, and delayed disclosure predicts PTSD development. [40][41][42][43] Interestingly, reduced trauma disclosure cannot completely explain the loneliness-associated increase in intrusive thoughts observed in men, because high-lonely women also reported less trauma disclosure and experienced fewer intrusions than low-lonely women. In addition, www.advancedsciencenews.com www.advancedscience.com low-lonely women showed more intrusions than low-lonely men reflecting previously observed sex differences in intrusive thought formation. [26,44] In the current sample, in contrast to men, high-lonely women may be less vulnerable to traumainduced intrusions since they also indicated less desire to talk about the trauma film relative to low-lonely women. Thus, sex-specific vulnerability to psychopathology may also vary depending on psychological factors such as social connectedness. The observed pattern of results could be related to our prestratification strategy and the recruitment of healthy high-lonely individuals who may be more resilient than high-lonely individuals who developed a psychological disorder. Along these lines, the opposing loneliness-related associations in women and men may have contributed to the absence of significant sex differences in high-lonely individuals. Therefore, in the same way that loneliness results from a discrepancy between desired and actual social connectedness, a mismatch between the desired and achieved trauma disclosure may be particularly important for individuals to cope with intrusive thoughts.
The amygdala is a well-known processing hub of fear-related stimuli and amygdala hyperreactivity is a risk factor for as well as a consequence of trauma-related disorders. [45][46][47][48][49] Sexdifferences in amygdala lateralization and habituation have been previously observed, with women exhibiting more activity in the left hemisphere related to the subsequent memory for emotionally arousing images and showing more persistent bilateral amygdala responses to negative stimuli than men. [27,50,51] Intriguingly, low-lonely women showed significantly less amygdala habituation and experienced significantly more intrusions than low-lonely men and increased amygdala habituation correlated with reduced intrusions across groups. Our findings are consistent with previous studies that showed that decreased amygdala habituation is associated with heightened anxiety levels and PTSD symptom severity. [52][53][54][55][56][57] Furthermore, increased functional connectivity between the amygdala and the superior parietal lobe in high-lonely men may constitute a prospective risk factor for heightened intrusive thoughts since the parietal lobe is part of a common network responsible for intrusive thought formation. [58] Moreover, PTSD patients exhibit increased parietal activations during script-driven trauma imagery leading to dissociative responses. [59] Furthermore, high-lonely men exhibited heightened amygdala responses to the CS+ and functional connectivity with the orbitofrontal cortex during conditioning compared to lowlonely men. Both amygdala and orbitofrontal cortex activity have been frequently linked to CS+/CS− differentiation during fear learning. [60] Nevertheless, increased amygdala responses to the CS+ were not reflected in significantly altered electrodermal activity (cf. the Supporting Information), indicating that the loneliness-related amygdala changes may be related to salience rather than arousal effects. The loneliness-related amygdala activation changes during habituation and conditioning in men were evident across social and nonsocial stimuli, which is in line with previous studies suggesting that loneliness fosters hypervigilance for threat cues. [4,61,62] Interestingly, high-lonely women compared to high-lonely men showed decreased left amygdala habituation to all faces, but we found decreased right amygdala habituation in response to social threat cues in high-lonely men. Impaired right amygdala habituation has also been previously identified as a neural phenotype of patients with borderline personality disorder and trauma exposure. [56] The absence of amygdala hypervigilance in high-lonely women could be driven by hormonal factors with high-lonely women showing increased estradiol levels compared to low-lonely women in our sample. Estradiol administration improved extinction recall after fear extinction, [63,64] and low levels of estradiol in women were linked to increased fear network responses to trauma films. [65] However, the observed sex differences cannot be completely explained by hormonal factors either because women reported more intrusions across loneliness groups despite having higher estradiol levels than men. It is conceivable that the content of the trauma film was more distressing for women than men, but we did not detect significant sex differences in the acute stress responses, and a previous study found no evidence for an interaction between sex and intrusive memories induced by different trauma films. [66] The unwillingness of men to admit loneliness and higher stigmatization of men who express feelings of loneliness might have contributed to the observed sex differences. [67,68] Taken together, our data suggest that loneliness has a sex-specific impact on the way threat cues are processed during fear conditioning and fear habituation.
The present study had several limitations. First, our sample consisted of women with and without hormonal contraception. Although we did control for the use of hormonal contraception and measured hormonal blood levels to control for menstrual cycle-related hormone changes, future studies are warranted to further delineate the hormonal basis of sex differences in the effects of loneliness. Second, the experimental trauma paradigm is widely used and well established to explore the neurobiological mechanisms underlying acute and prolonged trauma responses, but further clinical studies in a real-life setting are required to gauge whether our findings can be extrapolated to patients with trauma exposure. Third, while we found sex-specific associations between loneliness and amygdala reactivity consistently in two separate fMRI tasks, the results should be interpreted cautiously. The results were based on region of interest (ROI) analyses with lenient small-volume corrections and the effects sizes were small. Replication studies are warranted to test the robustness of these effects. Fourth, we used a prestratification approach and we only included individuals without current psychological disorders. This way we were able to exclude possible confounding effects due to current psychotherapy or pharmacotherapy. Nevertheless, given that loneliness is closely linked to mental health, the absence of loneliness-related effects in women may also reflect increased resilience in the group of high-lonely women.
Collectively, our results provide evidence that loneliness may confer vulnerability to increased intrusive thoughts in men following an experimental trauma. In addition, high-lonely men were characterized by an increased desire to talk about the trauma film and reduced actual trauma disclosure. This phenotype relates to altered limbic processing driven by amygdala hyperreactivity during fear conditioning and habituation. Based on these findings, secondary prevention strategies should take sex differences in loneliness into account and focus on improving the social connectedness of high-lonely men to mitigate the sequelae of traumatic experiences.

Experimental Section
Participants: The present study used a quasi-experimental design with a sample of prestratified healthy volunteers scoring high or low on the revised UCLA Loneliness Scale (UCLA LS). [69] High scorers (high-lonely) were defined by a score above or equal to 50 (i.e., at least one standard deviation above the mean score of healthy young adults, [70] which is similar to previous categorizations), [71] while low scorers (low-lonely) were defined by a score of 25 or below (i.e., at least one standard deviation below the mean). In total, 4515 participants completed the UCLA LS online questionnaire and a clinical screening interview was conducted with 97 subjects fulfilling the above-mentioned loneliness criteria. The final sample consisted of 82 healthy subjects (mean age ± standard deviation (SD): 26.39 ± 5.83 years) assigned to either a high-loneliness (n = 47 (20 women)) and a low-loneliness control group (n = 35 (18 women)). In accordance with our preregistration, every subject included in the final sample was aged between 18 and 46 years and had no current physical or psychiatric disorder as assessed via self-disclosure and the Mini-International Neuropsychiatric Interview, [72] no current psychotherapy, no current psychotropic medication, no illicit drug use in the previous four weeks, and was eligible for magnetic resonance imaging scanning (no pregnancy, metallic implants, etc.). All participants gave written informed consent. The study was approved by the institutional review board of the medical faculty of the University of Bonn (number 248/16) and carried out in compliance with the latest revision of the Declaration of Helsinki.
Experimental Design: In screening sessions, medical history and psychiatric symptoms were assessed (see the Supporting Information for inclusion criteria and Figure S1, Supporting Information, for a design overview). The testing session consisted of an fMRI scan containing a high-resolution structural scan, a fear COND/EXT paradigm, [73] and a well-established emotional face-matching paradigm. [74] All magnetic resonance imaging (MRI) data were acquired using a 3T Siemens TRIO MRI system (Siemens AG, Erlangen, Germany) with a Siemens 32-channel head coil. Following fMRI acquisition, the participants completed an experimental trauma paradigm. [28] To measure trauma disclosure and intrusive thoughts, subjects completed online diaries during the following three days after trauma exposure. Saliva samples were collected before the fMRI scan as baseline measure, and before and after the experimental trauma paradigm to measure oxytocin levels. In addition, blood samples were taken before the fMRI scan to measure the levels of gonadal steroids including estradiol and testosterone, as control variables. For a detailed list of the questionnaires and neuroendocrine parameters see the Supporting Information.
Emotional Face Matching Task: The first fMRI paradigm consisted of an adapted version of a well-established emotional face-matching paradigm. [74,75] Subjects had to match two simultaneously presented pictures at the bottom with a target picture presented at the top of the screen. Stimuli consisted of pictures of faces (neutral, fearful, and happy) and houses as nonsocial control stimuli. Stimuli were presented in three blocks for every condition (happy, fearful, and neutral faces, as well as houses), with each block consisting of five trials. Participants had to match the face identity (i.e., the emotion was consistent across all faces of a trial).
Fear Conditioning and Extinction Tasks: The COND/EXT paradigm was an adapted version of a Pavlovian fear conditioning paradigm described by Eckstein et al. [73] In the COND phase, subjects were shown four different pictures (two neutral faces (social stimuli) and two houses (nonsocial stimuli)). One social and one nonsocial pictures were designated as fearassociated CS (CS+) and the other picture of each category as safety signal (CS−). The choice of the picture that served as CS+ was counterbalanced within each group (high-lonely, low-lonely). Each stimulus was presented 16 times during the COND and EXT experiments. The trials were interleaved with an interstimulus interval (ISI) that was jittered between 5 and 7 s (mean: 6 s). In 75% of CS+ trials, subjects received an electric impulse (the UCS) 4 s after stimulus onset. The electric impulses were delivered by a Biopac System (MP150, Biopac Systems Inc., Goleta USA). To identify a stimulation intensity that was uncomfortable, but not painful, participants rated different intensities beforehand in an adaptive process (see the Supporting Information) while lying in the MRI on a scale from 0 to 100 (0 = not uncomfortable; 100 = most uncomfortable feeling imaginable). The stimulation intensity was set to reflect a rating of 60. In addition, the Biopac system measured electrodermal activity (EDA) and respiration during the experiment. After the COND phase, participants were informed that there would be another round of the same experiment. No electrical impulses were administered in the EXT phase. In both phases, participants had to press a button before the UCS to indicate if they believed that they would receive an electric impulse (i.e., a contingency rating was coded by +1 for an expected shock and −1 for no shock). For a detailed description of the data acquisition, preprocessing, and analyses of both tasks (see the Supporting Information).
Experimental Trauma Paradigm: Participants were seated in front of a Tobii TX300 binocular eye-tracker (Tobii AB, Danderyd, Sweden) with a 23 in display to measure pupil sizes as the outcome indicating physical arousal during the movie alongside EDA. To evoke intrusive thoughts, participants were confronted with a 24-min-long movie clip derived from the movie "I spit on your grave" showing the multiple rape of a young woman by a group of men. EDA data were measured with a Biopac MP150 system. Positive and negative affect, dissociative symptoms (measured with the dissociative symptoms scale), [76] valence (0 = low valence, 100 = high valence), arousal (0 = low arousal, 100 = high arousal) as well as state anxiety were measured prior and after the experimental trauma paradigm. The participants completed online intrusion diaries at home in the evening during three consecutive days following trauma exposure. For details about data collection and preprocessing, see the Supporting Information.
Online Diaries: The participants completed online intrusion diaries at home in the evening for three consecutive days after trauma exposure. In the intrusion diary, the participants stated the number of intrusions (defined as involuntary recollections relating to film events that appear, apparently spontaneously, in consciousness) and rated the distress caused by each of these intrusions on a visual analogue scale ranging from 0 (no distress) to 100 (extreme distress). Furthermore, participants were asked to state the time spent on talking about the trauma video (in minutes) and their desire (0 = no desire to 100 = strong desire) for trauma disclosure.
Statistical Analyses: The primary outcomes included the number of intrusions and blood oxygen level-dependent signal changes during fear learning and the processing of fearful faces. Fear habituation was assessed in an exploratory analysis. Other outcomes recorded were the psychological and physiological stress markers after trauma exposure and skin conductance response during fear conditioning. Mixed-design analyses of variance (ANOVAs) and Bonferroni-corrected (p cor ) post hoc t-tests were calculated using SPSS 25 (IBM Corp., Armonk, NY, USA) to examine changes in intrusive thoughts (sum of the three consecutive days following the trauma exposure), trauma disclosure (i.e., how long participants talked to other people and whether and how long they discussed the trauma movie with other people), group differences in psychiatric symptoms and psychological as well as physiological and hormonal responses to the trauma exposure with the between-subject factors of sex (women, men) and loneliness (high, low). Mixed-design ANOVAs for contingency ratings included the additional within-subject factors task (COND, EXT) and condition (CS+, CS−). Additional mixed-design ANOVAs for the COND/EXT paradigms included the between-subject factors of sociality (social, nonsocial) and time (first half, second half). Partial eta-squared and Cohen's d were calculated as measures of effect size.
To analyze the fMRI data, a two-stage approach was used as implemented in the MATLAB toolbox (The MathWorks Inc., Natick, MA) SPM12 (Wellcome Trust Center for Neuroimaging, London, UK; http://www.fil. ion.ucl.ac.uk/spm). On the first level, data were modeled using a fixedeffects model. On the second level, the main contrasts of interest were compared between groups using a full factorial model with the two factors of loneliness and sex. Analyses were conducted using anatomically defined regions of interest (ROIs), including the amygdala, derived from the WFU PickAtlas (for further ROI results, see the Supporting Information). P values smaller than 0.05 after familywise error correction for multiple testing (p FWE ) based on the size of the ROI (i.e., small volume correction for separate ROIs) were considered significant. Whole-brain analyses were calculated across groups for task-validation (cluster defining threshold p < 0.001; significance threshold p FWE < 0.05 corrected at peak level). In addition, generalized psychophysiological interaction analysis was conducted to assess functional connectivity by using the CONN toolbox 18.a (www.nitrc.org/projects/conn, RRID: SCR_009550) with the same preprocessed data, ROIs, regressors, and contrasts that were used in the SPM analyses. [77] Parameter estimates of significant contrasts were extracted using MarsBar (https://www.nitrc.org/projects/marsbar, RRID: SCR_009605) and further analyzed in SPSS 25 ). Pearson correlations between parameter estimates of significant ROI clusters and intrusive thoughts were calculated.

Supporting Information
Supporting Information is available from the Wiley Online Library or from the author.