Basic research and clinical exploration of cold atmospheric plasma for skin wounds

Abstract Skin wounds, such as burns, diabetic foot ulcers, pressure sores, and wounds formed after laser or surgical treatment, comprise a very high proportion of dermatological disorders. Wounds are treated in a variety of ways; however, some wounds are greatly resistant, resulting in delayed healing and an urgent need to introduce new alternatives. Our previous studies have shown that cold atmospheric plasma (CAP) has antibacterial activity and promotes cell proliferation, differentiation, and migration in vitro. To further advance the role of CAP in wound healing, we evaluated the safety and efficacy of CAP in vitro by irradiation of common refractory bacteria on the skin, irradiation of normal skin of rats and observing reactions, treatment of scald wounds in rats, and treating clinically common acute wounds. Our findings revealed that CAP can eliminate refractory skin bacteria in vitro; CAP positively affected wound healing in a rat scalding wound model; and direct CAP irradiation of low intensity and short duration did not lead to skin erythema or edema. CAP promises to be a new, economical, and safe means of wound treatment.


| INTRODUCTION
As the body's largest organ and first line of defense, the skin performs multiple important functions, including providing a physical barrier against pathogens and performing absorption, secretion and excretion, thermoregulation, sensory, immune, respiratory, and endocrine functions, as well as participating in various functional activities of the entire body and maintaining an environment of stability within the body. 1 Skin is highly susceptible to numerous factors that can lead to tissue damage and the onset of trauma.Acute wounds are very common in dermatology clinics, such as those resulting from trauma, burns, laser treatment, cryotherapy, or surgery.Most acute wounds heal quickly with early and aggressive intervention.However, some wounds become chronic due to delayed healing caused by local ischemia or wound infection, especially complicated infections. 2 In addition to high morbidity and recurrence rates, wounds are often accompanied by high-cost expenditures, which not only impose a considerable economic burden on patients, but also put tremendous pressure on national health care systems.
Wound repair is a complex series of distinct but partially overlapping processes, including phases of coagulation, inflammation, proliferation, and remodeling phases.There are multiple cellular interactions involved, including those between inflammatory cells, fibroblasts, keratinocytes, and endothelial cells, along with growth factors and enzymes. 3,4Debridement, antiseptics, wound dressings, and negative pressure therapy are common ways of treating wounds.
6][7] However, painful treatment, repeated medical visits, and the great expenses involved in treatment lead to poor patient compliance.In addition, the emergence of antibiotic resistance further increases delays in the healing process. 8,9Therefore, more effective treatment pathways are urgently needed.Exploring new skin trauma treatment methods, optimizing treatment protocols, and improving the effectiveness of trauma treatment are the main research directions for clinicians and researchers.
Plasma is a neutral, ionized gas that can be created under natural or artificial conditions.Due to its unique physical and chemical properties, it has applications in areas such as electrical, physical, chemical, energy, environmental, biological, medical, and materials science.
CAP-treated biological dressings showed even better biological effects 41,42 ; and other new CAP treatment modalities, such as plasma knife and plasma cupping, are now also being developed. 43,44e most widely used CAP application is the healing of different wound types.Researchers have found that CAP is essential for wound healing, but the specific cellular and molecular mechanisms by which it promotes tissue repair still need further investigation.In the present study, we investigated the antibacterial effect on the skin surface, the stimulatory response of CAP on local skin, and the therapeutic effect of CAP on clinical acute wounds.Based on the results of in vivo and ex vivo experiments, we concluded that CAP is expected to be a novel effective treatment modality for skin wounds.

| CAP effectively kills common bacteria on refractory wounds
Four common refractory wound bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter baumannii) were treated with CAP in vitro.The number of surviving bacteria changed after irradiation at different times (30, 60, 90, 120 and 180 seconds) as shown in Figure 1 (the number of colonies counted by the plate counting method was converted into logarithmic values).
After 24 h of bacterial culture, the initial bacterial count was 6.0

| Observation of CAP irradiation on local skin in rats
We show the irritation scores of CAP irradiated rat skin in Tables 1   and 2, and images of localized skin irritation in rats by CAP in Figure 2, and show the skin histomorphometric results in Figure 3.In the high-intensity CAP treatment group, when the irradiation time was 30 min, all three rats showed irritation reactions (erythema, edema, or both) on the dorsal skin, and the high-intensity CAP irradiation of the skin for 30 min was assessed as moderately irritating.
When the irradiation was continued for 20 min, one rat showed slight erythema on the skin of the experimental site, which was assessed as mildly irritating.No rats showed skin abnormalities at the experimental site when irradiated for 10 min or 5 min, which was assessed as nonirritating.In the low-intensity CAP treatment group, no significant irritation was observed even after 30 min of continuous CAP skin irradiation.Histopathology showed a slight cutaneous edema after high intensity CAP treatment compared to the normal skin, while there was no significant difference in inflammatory cells.
T A B L E 2 Skin irritation index score in CAP-irradiated rats.healing between the two CAP groups of different intensities.The histomorphological results showed that on day 5, inflammatory cell infiltration was visible in all groups, and neovascularization and skin accessory structures such as hair follicles and sebaceous glands were visible in varying degrees in the CAP treatment group but not in the control group; on day 10, neocytes and blood vessels were visible in the control group, but the skin structure hierarchy was disordered; in the CAP treatment group, epithelial cells were fully differentiated, with a clearly visible cell hierarchy.On day 15, the epidermal layer of the control group was clear, and the proliferated fibers were disorganized; in the CAP treatment group, the cell layer was clear, and the fibers were proliferated and neatly arranged; on day 20, the formation of blood vessels and epithelial differentiation of the traumatic surface were visible in all groups, and the physiological structure of the skin was restored (Figure 5).Case 1: A 10-year-old male patient presented to our department with localized scar hyperplasia and underwent fractional CO 2 laser treatment.However, after laser treatment, the wound developed breakdown and oozing.After full communication with the patient's family, CAP treatment was agreed upon.After four times of CAP treatment, the wound exudation decreased and some of the wounds crusted over.After 9 consecutive days of treatment, the wound had healed significantly.After a total of 22 times of CAP treatment, the wound was healed (Figure 6).
Case 2: A 73-year-old woman was diagnosed with hand eczema and was treated with a combination of glucocorticoid ointment and cryotherapy, after which hand ulcers developed.After communication with the patient, CAP treatment was initiated.At the end of the second CAP treatment, wound exudation was significantly reduced, and the wound was completely healed after nine CAP treatments (Figure 7).Case 6: A 67-year-old male with poorly healed wounds after varicose veins in the lower extremities had been treated with fibroblast growth factor gel and antibiotic ointment combined with infrared irradiation, with no significant improvement of the ulcerated surface; the patient was managed with 14 times of CAP treatment and the wounds healed without significant scar growth (Figure 11).

| DISCUSSION
Wound healing is a complex process, and different parts of the wound may show different stages of healing, making it difficult for the ideal synchrony to be achieved between the various parts of the wound.and rapidly develop multiple drug resistance, 45 leading to high morbidity and mortality and increasing the economic and psychological burden on patients. 468][49] With the increase in drug-resistant bacteria and infections caused by complex bacteria, the single use of antibiotics often fails to achieve the desired therapeutic effect. 50,51 this study, the number of surviving colonies of S. F I G U R E 5 Representative histological features of rat skin: from top to bottom are the images of rat skin on day 5, 10, 15, and 20, respectively, with the control, low-intensity CAP, and high-intensity CAP groups, respectively in each row from left to right (yellow, red, green, black, and blue arrows point to inflammatory cells, neovascularization, hair follicles, sebaceous glands, and fibrous components, respectively).The magnification of these images is 100Â.CAP, cold atmospheric plasma.
T A B L E 3 Patient clinical data and treatment process.Burns are the most common type of acute trauma and are more prevalent in low socioeconomic level populations and less developed areas.When skin integrity is disrupted, the large amount of necrotic tissue and protein-rich exudate at the damaged site provides the energy needed for microbial proliferation, leading to increased microbial susceptibility at the trauma site.In addition to infection, burns lead to a variety of other unavoidable complications, including scarring and impairment of limb function, requiring long-term rehabilitation, reconstruction, and anti-scar therapy. 53Moreover, it has been reported that burns are associated with an increased risk of cardiovascular disease, neurological disease, musculoskeletal disease, gastrointestinal disease, diabetes, infections, anxiety, depression, and tumor growth. 54In this study, we conducted a preliminary exploration of the effectiveness of air-sourced CAP for treating scald wounds in rats, with daily CAP treatment for 3 min at a time for 20 days.Wound healing was found to be faster in the CAP group compared to the control group.The histomorphological rat skin findings showed that CAP treatment had a positive effect on the formation of blood vessels and granulation tissue and accelerated the re-epithelialization process.
Similarly to our results, He et al. 17 treated diabetic mice with helium, 90-s CAP, or 180-s CAP for 14 days and showed that CAP improved wound healing without a difference between exposure times.
Lou et al. 26 found that CAP treatment resulted in a significant reduction in wound area and a decrease in inflammatory cell infiltration compared to the control group.In another study, a single CAP treatment resulted in significantly faster wound healing, while CAP combined with negative pressure therapy and bone marrow mesenchymal transplantation further accelerated the healing process. 27These reports suggest a possible synergistic effect of CAP when combined with other therapies to promote wound healing.As a limitation of our study, we did not examine the mechanisms of CAP-associated wound healing at a specific molecular or genetic level.Nonetheless, researchers have found that CAP enhances wound healing primarily by promoting angiogenesis, accelerated NO synthesis, and enhanced expression of PDGFR and CD31 pro-angiogenic markers. 28Kisch et al. 29 found that CAP can regulate tissular oxygen pressure and improve skin microcirculation, which is another key factor in wound healing and angiogenesis.Additionally, CAP promotes wound healing by regulating collagen deposition, reducing extracellular matrix degradation, and regulating wound healing stage-dependent proteases [e.g., matrix metalloproteinases (MMPs)]. 30 future studies, we will further explore the mechanisms by which CAP promotes wound healing.infection, and delayed wound healing have never been completely avoided.In 2017, Hartwig et al. 55 used CAP to treat difficult-to-heal wounds after head and neck surgery and achieved satisfactory results.
In our study, using CAP for acute wounds formed after trauma, cryotherapy, laser treatment, or surgery, we found a positive effect of CAP on acute wounds, especially on the healing of complex acute wounds.National and international research findings confirm the differential effect of gender on the trauma healing process.Female mice showed an advantage in the trauma healing process, which was associated with the upregulation of adenosine A 2A receptor-mediated angiogenesis by 17βestradiol 56 ; Routley et al. 57 found that estrogen and progesterone drive wound repair, angiogenesis and remodeling through activation of macrophages.Furthermore, Gilliver et al. 58 showed increased type I collagen and fibronectin deposition in the wounds of male denuded rats compared to normal male rats, accompanied by a significant decrease in the activity of collagen digesting enzymes (MMP-2 and MMP-9).In general, estrogen was identified as a repair promoter that accelerates healing, whereas androgens are an inhibitor that delays injury recovery.In addition to sex hormones, the influence of gender on wound healing is manifested in other factors that are so complex that male and female organisms respond to injury repair, which is itself different.In another study by Gilliver et al., 59 they showed the differences in the area of unhealed wounds in ovariectomized females and male denuded animals, which may be related to a combination of macrophage migration inhibitory factor as well as unknown factors.In preclinical studies, to observe the outcome of CAP on wound repair, we used male Sprague Dawley (SD) rats to avoid the effect of sex differences on wound healing.As far as the results of the current study are concerned, CAP seems to have a consistent effect with estrogen on wound healing, and it is equally beneficial in accelerating wound healing.However, few animal experiments on the effect of CAP on wound healing have been observed with female animals as the study subjects.Whether CAP has an antagonistic effect with estrogen and thus has a negative effect on wound healing is currently lacking, and we will further improve this part in future studies.
Multiple studies have confirmed the ability of CAP to induce mutations, apoptosis, and DNA damage in the treatment of neoplastic diseases, but whether CAP is harmful to humans, cells, or tissues deserves more attention.Maisch et al. 60  models (in vitro culture dishes, animal models, and for biomedical applications).How to compensate for these differences considering the currently available in vitro and ex vivo evidence is critical to standardize treatment modalities for the safe application of CAP in biomedicine and to reduce any potential risks. 63asma medicine is continuing to evolve rapidly, and increasingly more studies are providing new evidence for the use of CAP as an alternative treatment for a variety of skin trauma and skin diseases.
CAP is sensitive not only towards postsurgical wounds, laser, and cryotherapy wounds, but can also be used in the treatment of a wide range of skin diseases.This study confirms the potential application of CAP in wound healing, and CAP is expected to become a novel, economical, and safe treatment modality.However, the study also had

| CONCLUSIONS
CAP has an in vitro sterilizing effect on common skin traumarefractory bacteria (S. aureus, E. coli, P. aeruginosa, A. baumannii).Low intensity and brief CAP irradiation of normal skin does not lead to reactions such as erythema or edema, but high intensity and prolonged irradiation shows erythema and edema irritation reactions, and the probability and severity of irritation reactions increase with treatment time.CAP can promote the healing of local scald wounds in rats.
Regarding the treatment effect of CAP on clinical common skin wounds, patients have high acceptance and mild adverse reactions, which has a positive impact on shortening the course of treatment.
However, there is no accurate standard for the therapeutic dose of CAP for different types of wounds.Accurately controlling the therapeutic dose also remains a challenge which needs to be further explored and discovered in the future.

| Plasma equipment
The ST-P101 plasma skin therapy instrument (Hefei CAS Ion Medical and Technology Devices Co., Ltd, Hefei, China) was used in this study (Figure 12).High voltage electrodes (copper needles with a tip radius of approximately 1 mm) are required, and a handheld electrode sheet is connected to the experimental animal limb, forming a closed loop.
Plasma is generated through a high voltage electrode discharge with an adjustable voltage control range of 9-15 kV and a ballast resistance of 10 MΩ.During the experiment, the distance between the skin surface of the treated area and the multielectrode tip was kept at 10 mm, perpendicular to the skin surface.The plasma discharge voltage and current were monitored using an MSO 5104 digital oscilloscope equipped with a high voltage probe (P6015A) and a current probe (Tektronix P6021, Tektronix, Bracknell, United Kingdom); the current and voltage of the monitored plasma are shown in Figure 13.
The emission spectra were recorded using an AvaSpec-2048 spectrometer (Avantes, Apeldoorn, The Netherlands); the emission spectra of the plasma are shown in Figure 14.At the start of the experiment, the weight of each rat was measured using an electronic scale.Each rat was anesthetized intraperitoneally using 40-50 mg/kg of 2% pentobarbital sodium.After successful anesthesia, the back of the rat was dehaired.The intensity of the CAP device was set at 80% (energy output 0.8 J/s) and the electrode piece was suspended at 1 cm from the surface of the rat's skin.Twelve adult rats were irradiated for 30, 20, 10, or 5 min; irradiation was repeated for the three rats in each group.The above treatment step was repeated at 60% (energy output 0.4 J/s).The skin surface damage (erythema or edema) of rats in each group was observed immediately after treatment, 24 h after treatment, and writingreview and editing (equal).

ACKNOWLEDGMENTS
We thank the patients and their families for their participation in this study.

FUNDING INFORMATION
This Changes in the number of viable colonies after 30, 60, 90, 120, and 180 s of exposure to CAP with four types of bacteria in refractory wounds.(a) Staphylococcus aureus, (b) Pseudomonas aeruginosa, (c) Escherichia coli, and (d) Acinetobacter baumannii.log 10 CFU/mL, and the number of bacteria gradually decreased with the extension of the CAP treatment.When using CAP treatment for 90 s, the number of surviving colonies was approximately 4.0 log 10 CFU/mL, while with 180 s of treatment, three bacterial species were almost killed, with the exception of A. baumannii.The results show that CAP has a sterilization effect in vitro.

2. 4 |
Observation of CAP with the treatment of clinical acute wounds CAP was used to treat six clinical acute wounds (one case of trauma formation after laser treatment, two cases of post-freezing wounds, and three cases of postsurgical wounds).The patients' clinical data and treatment course are shown in Table 3.It was found that after 8-22 times of CAP treatment, all wounds were nearly cured.One patient had only three times of CAP treatment because of the distance to the F I G U R E 2 Local skin images of rats after receiving lowintensity and high-intensity CAP irradiation for 5, 10, 20, and 30 min, respectively.CAP, cold atmospheric plasma.F I G U R E 3 HE staining of erythematous or edematous skin tissues, with enlarged cell gaps indicated by blue arrows and inflammatory cells shown by red arrows.The magnification of these images is 100Â.HE, hematoxylin-eosin.place of residence, but the wound healed effectively.All patients who received treatment reported either no or tolerable pain during treatment.No skin irritation reactions such as erythema and edema were observed in all patients.

Case 3 :
An 88-year-old woman developed a right temporal cutaneous horn.She declined surgical treatment and requested local cryotherapy; however, ulceration of the wound occurred after treatment.After communication with the patient, CAP treatment was performed, and the wound healed completely after eight times of CAP treatment (Figure8).

Case 4 :
A 74-year-old woman was diagnosed with facial basal cell carcinoma and subsequently underwent an enlarged mass excision and adjacent metastatic flap repair.However, the wound healed poorly after surgery.After communication with the patient, CAP treatment was performed once a day, and the wound healed significantly after three times of consecutive CAP treatment (Figure9).

Case 5 :
A 77-year-old woman was diagnosed with actinic keratosis of the left temporal region.The patient underwent surgical resection, but healing was not successful.After 10 times of CAP treatment, the wound healed (Figure10).
The "ESKAPE" (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) group was first introduced in 2008 to describe treatment-resistant and life-threatening Gram-positive and Gramnegative bacterial pathogens that are often insensitive to antibiotics Surgery, laser therapy, and cryotherapy are common clinical treatments in dermatology.With technological advances, dermatological surgery and dermatological aesthetics have gained unprecedented opportunities for development.However, the adverse effects of pain, F I G U R E 6 Images of wound healing before and after CAP treatment.(a) Before the treatment; after receiving (b) 1, (c) 7, (d) 12, (e) 18, and (f) 22 times of CAP treatment.CAP, cold atmospheric plasma.

F I G U R E 7
Images of wound healing before and after CAP treatment.(a) Before the treatment; after receiving (b) 2, (c) 4, (d) 5, (e) 8, and (f) 9 times of CAP treatment.CAP, cold atmospheric plasma.F I G U R E 8 Images of wound healing before and after CAP treatment.(a) Before the treatment; after receiving (b) 2, (c) 3, (d) 5, (e) 7, and (f) 8 times of CAP treatment.CAP, cold atmospheric plasma.

F I G U R E 9
Images of wound healing before and after CAP treatment.(a) Before the treatment; after receiving (b) 1 and (c) 3 times of CAP treatment.CAP, cold atmospheric plasma.F I G U R E 1 0 Images of wound healing before and after CAP treatment.(a) Before the treatment; after receiving (b) 1, (c) 3, (d) 5, (e) 7, and (f) 10 times of CAP treatment.CAP, cold atmospheric plasma.
treated keratinocytes and fibroblasts with CAP for 2 min and no relevant toxic effects were observed.After repeated treatment with CAP for 2-10 min every 24 h for 5 days, no mutagenicity was detected in a mammalian cell gene mutation assay, while UV-C exposure of cells induced DNA damage and mutagenesis.Schmidt et al.61 performed the first longterm analysis of a mouse model (1 year of continuous follow-up after completion of CAP treatment) and described the CAP-treated traumatic areas without abnormal morphological changes histologically, no excessive scarring, or chronic inflammatory response; histology and radiology (magnetic resonance imaging) showed no malignant tumorigenesis in skin tissue or solid organs.Evert et al.62 also found that repeated CAP exposure did not lead to noninvasive lesions or squamous cell carcinomas in the oral mucosa of mice.Different intensities of CAP were used to irradiate the normal dorsal skin for different time periods, and it was found that local skin erythema and edema appeared with increasing treatment time and intensity.At high intensity, the treatment time of 10 min was safe, and skin biopsy of the area where erythema and edema appeared revealed slight skin keratinization and edema, with no significant differences in inflammatory cells; with low-intensity CAP, no skin irritation reaction was observed even after 30 min of continuous irradiation.After repeated validation, no significant adverse effects were found with the plasma devices used in the clinic and laboratory.However, CAP exhibits different electrical properties in different F I G U R E 1 1 Images of wound healing before and after CAP treatment.(a) Before the treatment; after receiving (b) 3, (c) 6, (d) 8, (e) 11, and (f) 14 times of CAP treatment.CAP, cold atmospheric plasma.
the following limitations:(1) the CAP device used in this study only confirmed its efficacy and safety in trauma treatment through in vitro bactericidal effects and studies in rat scald models, and lacks more indepth mechanistic studies; (2) only male rats were observed for the study in the animal experiments, and whether there are gender differences in the effect of CAP on wound healing needs to be further explored;(3) no further studies on microbial infection of trauma and posttreatment conditions were conducted in the clinical study; (4) in the clinical study, patients had received various other treatments before CAP treatment, which may affect the final result;(5) the clinical sample of this study was very limited.To further confirm the efficacy and safety of CAP treatment, additional clinical samples should be included, randomized controlled observational studies should be conducted, and the treatment parameters should be explored.

5. 2 | 5 . 3 |
Inactivation of common bacteria in refractory wounds by plasmaTryptone, yeast extract, sodium chloride, and agar were added to deionized water in a concentration ratio of 10, 5, 10, and 20 g/L, respectively, sterilized at 121 C for 30 min, poured onto a 60-mm sterile flat plate, left to cool and solidify, and then prepared for use.Further, 2 mL of bacterial suspension in 35-mm sterile petri dishes was irradiated with CAP for 0, 30, 90, 120, and 180 s, respectively.Using a micropipette, 100 μL of diluted bacterial solution were added to the Lysogeny broth solid medium, spread evenly with a sterile coating rod, and then placed into a constant temperature incubator at 37 C for 24 h.The total number of surviving bacteria was obtained by counting the number of surviving bacterial colonies in the Petri dishes multiplied by the dilution factor.Observation of the effect of plasma irritant on local skin in rats SD rats (SPF grade, 180-220 g, males) were purchased from Zhejiang Viton Lihua Laboratory Animal Technology Company and housed in a standard breeding environment.Twenty-four SD rats were numbered from 1 to 24 according to the body weight, and the random numbers were obtained using Excel software, so that each animal was coded with a different random number.The animals were reordered by "increasing" random numbers, and then divided into eight groups in sequential order with three replicates in each group.CAP irradiation was performed according to the treatment intensities and treatment durations specified in Table4.The use of research animals in this study was reviewed and approved by the Laboratory Animal Welfare and Medical Ethics Committee of Anhui Medical University (No. LLSC20221118).

5 . 4 | 5 . 5 |
48 h after treatment.The severity of erythema and edema was scored according to Table5(skin irritation response scoring criteria), and the skin irritation index was calculated by averaging the accumulated irritation scores of all animals in each group.The skin irritation intensity after CAP irradiation was assessed according to Table6(skin irritation intensity grading).After the observation, the skin tissues of rats with skin irritation reactions were fixed using 4% paraformaldehyde fixation, stained with hematoxylin-eosin (HE), and examined histomorphologically.The pathological tissue samples were stained and fixed by the Wuhan Safeway Biotechnology Company.Observation on the efficacy of plasma treatment for the scalding model in rats Forty SD rats were dehaired 24 h before the experiment.To establish the scald model, a metal cylinder with a base diameter of 15 mm was heated at a constant temperature of 85 C in water for 15 min.Next, the metal was placed on the left and right sides of the dehaired back of the rats for 5 s without pressure, and each rat had two identical traumas on the dorsum.Forty rats were sorted by body weight, and two rats with approximately the same body weight were paired and numbered 1 and 2, respectively.Twenty numbers were copied from the random number table.For odd numbers, the animal numbered 1 in the pairing group entered the high-intensity CAP group and the animal numbered 2 entered the low-intensity CAP group, and for even numbers, the opposite is true, so that 40 rats were randomly divided into two groups.The left side of each rat was coated with saline as the control group, and the right side was irradiated with high-intensity and lowintensity CAP according to the group.The irradiation method was the same as that of CAP irradiation in the study of rat skin safety and CAP treatment was performed once a day for 3 min at a time for 20 days.The area of dorsal trauma was recorded on treatment days 1, 5, 10, 15, and 20, and the dorsal skin was taken for HE staining to observe the histomorphological changes.All procedures were done by the same experimenter to avoid additional errors caused by intersubject variation.Observation of plasma treatment with clinical acute wounds From December 2021 to February 2022, six patients with skin wounds that did not respond well to conventional treatment protocols were selected from our outpatient clinic and wards, and CAP treatment was administered after communication with patients and F I G U R E 1 2 (a) The air-sourced cold atmospheric plasma device.(b) High voltage discharge tip.F I G U R E 1 3 Current and voltage signal diagram of the plasma center point under specific parameters.F I G U R E 1 4 Emission spectra of plasma instruments.their guardians, obtaining written consent.Pregnant and lactating women, people with dementia, and those who asked to be withdrawn halfway through were excluded from the study.This study was ethically reviewed by the Ethics Committee of the Second Affiliated Hospital of Anhui Medical University (No. YX2021-029 (F1)).Patients were placed in an appropriate position for full exposure of the treatment area and the lesions were cleaned with saline to remove foreign bodies.The traumas are treated with the ST-P101 plasma skin treatment instrument.Different high voltage output electrodes are selected depending on the size of the lesion and the frequency of treatment depends on the severity of the wound (once a day or every other day) until the wound cured or nearly cured.A 30-min observation was undertaken after each treatment.Three indicators were used to evaluate treatment effectiveness: cured (disappearance of clinical symptoms and signs), effective (improvement of clinical symptoms and signs), and ineffective (neither improvement nor aggravation of clinical symptoms and signs).The presence of adverse reactions (itching, pain, erythema, or edema at the irradiated area) was used to evaluate treatment safety for the human body.The skin lesions were photographed before and after each treatment.5.6 | Statistical analysis Data were subjected to t-tests and nonparametric tests using IBM SPSS Statistics 26 (IBM Corp. Armonk, NY, USA) to assess differences between experimental groups.Results were considered statistically significant at p < 0.05.AUTHOR CONTRIBUTIONS Miaomiao Li: Data curation (equal); investigation (equal); methodology (equal); software (equal); writingoriginal draft (equal); writingreview and editing (equal).Jing Gao: Conceptualization (equal); data curation (equal); funding acquisition (equal); methodology (equal); software (equal); writingoriginal draft (equal); writingreview and editing (equal).Liyun Wang: Funding acquisition (equal); methodology (equal); writingreview and editing (equal).Jia Liu: Methodology (equal); writingreview and editing (equal).Chuyu Fu: Methodology (equal); writingreview and editing (equal).Xingyu Yang: Methodology (equal); writingreview and editing (equal).Shengquan Zhang: Conceptualization (equal); funding acquisition (equal); supervision (equal); writingreview and editing (equal).Xinwei Li: Methodology (equal); writingreview and editing (equal).Shengyong Luo: Methodology (equal); writingreview and editing (equal).Chunjun Yang: Conceptualization (equal); funding acquisition (equal); project administration (equal); supervision (equal);