Combined effects of elevated CO2 concentration and Wolbachia on Hylyphantes graminicola (Araneae: Linyphiidae)

Abstract The increasing concentration of carbon dioxide in atmosphere is not only a major cause of global warming, but it also adversely affects the ecological diversity of invertebrates. This study was conducted to evaluate the effect of elevated CO2 concentration (ambient, 400 ppm and high, 800 ppm) and Wolbachia (Wolbachia‐infected, W+ and Wolbachia‐uninfected, W−) on Hylyphantes graminicola. The total survival rate, developmental duration, carapace width and length, body weight, sex ratio, net reproductive rate, nutrition content, and enzyme activity in H. graminicola were examined under four treatments: W− 400 ppm, W− 800 ppm, W+ 400 ppm, and W+ 800 ppm. Results showed that Wolbachia‐infected spiders had significantly decreased the total developmental duration. Different instars showed variations up to some extent, but no obvious effect was found under elevated CO2 concentration. Total survival rate, sex ratio, and net reproductive rate were not affected by elevated CO2 concentration or Wolbachia infection. The carapace width of Wolbachia‐uninfected spiders decreased significantly under elevated CO2 concentration, while the width, length and weight were not significantly affected in Wolbachia‐infected spiders reared at ambient CO2 concentration. The levels of protein, specific activities of peroxidase, and amylase were significantly increased under elevated CO2 concentration or Wolbachia‐infected spiders, while the total amino content was only increased in Wolbachia‐infected spiders. Thus, our current finding suggested that elevated CO2 concentration and Wolbachia enhance nutrient contents and enzyme activity of H. graminicola and decrease development duration hence explore the interactive effects of factors which were responsible for reproduction regulation, but it also gives a theoretical direction for spider's protection in such a dynamic environment. Increased activities of enzymes and nutrients caused by Wolbachia infection aids for better survival of H. graminicola under stress.

and W + 800 ppm. Results showed that Wolbachia-infected spiders had significantly decreased the total developmental duration. Different instars showed variations up to some extent, but no obvious effect was found under elevated CO 2 concentration.
Total survival rate, sex ratio, and net reproductive rate were not affected by elevated CO 2 concentration or Wolbachia infection. The carapace width of Wolbachia-uninfected spiders decreased significantly under elevated CO 2 concentration, while the width, length and weight were not significantly affected in Wolbachia-infected spiders reared at ambient CO 2 concentration. The levels of protein, specific activities of peroxidase, and amylase were significantly increased under elevated CO 2 concentration or Wolbachia-infected spiders, while the total amino content was only increased in Wolbachia-infected spiders. Thus, our current finding suggested that elevated CO 2 concentration and Wolbachia enhance nutrient contents and enzyme activity of H. graminicola and decrease development duration hence explore the interactive effects of factors which were responsible for reproduction regulation, but it also gives a theoretical direction for spider's protection in such a dynamic environment.
Increased activities of enzymes and nutrients caused by Wolbachia infection aids for better survival of H. graminicola under stress.

| INTRODUC TI ON
Because of the extensive use of fossil fuels since the industrial revolution, the atmospheric CO 2 concentration has surged greatly from 280 ppm in the year 1700 to 400 ppm in the year 2015 (WMO, 2016). Elevated CO 2 concentrations have raised the global average temperature, which directly affected the nutrition and biomass of the plants and insects, and even affected the agro-ecosystems (Andresen et al., 2018;Lu et al., 2018). Although, previous studies have reported the effects of elevated CO 2 concentration on the reproductive parameters such as development, fecundity, and oviposition rate in Tetranychus urticae (Acari: Tetranychidae), Aphis gossypii (Homoptera: Aphididae), and Helicoverpa armigera (Lepidoptera: Noctuidae) (Gao et al., 2008;Joutei, Roy, Impe, & Lebrun, 2000).
However, these studies were mainly focused on the plants and insects, with little attention being focused on spiders.
Some studies have reported that elevated CO 2 concentration can have potential effects on spiders. Zuo et al. (2015) reported that the total developmental duration of spiderlings was significantly increased at elevated CO 2 concentration, while the body length and weight were decreased in a wandering spider, Pardosa astrigera (Araneae: Lycosidae). Unlike P. astrigera, when a web-weaving spider Agelena labyrinthica (Araneae: Agelenidae) was exposed to elevated CO 2 concentration, the total developmental duration was significantly decreased, while the carapace size and weight were not affected. These observations suggest that elevated CO 2 concentration has species-specific impacts on spiders (Wang et al., 2016).
Spiders can prey extensively on plant pests, decreasing the density of insects and stabilizing the populations, thereby effectively reducing the pesticide consumption (Maloney, Drummond, & Alford, 2003;Tanaka, Endo, & Kazano, 2000). In addition, spiders can be used as biological indicators to detect the heavy metals for monitoring environmental conditions (Li et al., 2016). They also have other advantages over other similar organisms. For example, the characteristics of greater resistance to temperature (low or high) and their higher resilience to starvation are uncommon in other predators (Cramer & Maywright, 2008;Henschel, Ward, & Lubin, 1992;Lowrie, 1980).
Hylyphantes graminicola is the dominant species of the agricultural fields in China. They have a small body size (2.5-5 mm), a relatively fast growth rate, easily reared, and can prey on an extensive range of pests, like aphids, leafhoppers, and corn borers (Zhao, 1993). These characteristics indicate that H. graminicola is an ideal species for research in this field. As a consequence, H. graminicola also plays an important role in controlling pests in the agro-ecosystems.
Wolbachia are maternally inherited Gram-negative bacteria found in the reproductive tissues of arthropods. They belong to α-subdivision of the Proteobacteria (Weisburg et al., 1989), and Wolbachia have attracted a considerable recent interest (Schuler et al., 2018). Interestingly, Duron et al. (2008) reported that the proportion of arthropod species have been infected from 22.8% to 32.4% in Western Europe, and such proportion had further increased to 40% (Zug & Hammerstein, 2012). Wolbachia are extensively widespread bacteria and their population has increased dramatically in nature.
Although Wolbachia had a widespread effect in arthropods (LePage et al., 2017), few studies had reported the presence of these bacteria in spiders. Previous studies on Wolbachia have investigated their role in evolutionary processes , reproduction and development (Yun, Yang, Wang, Peng, & Jiao, 2013), biological control (Kehlmaier, Michalko, & Korenko, 2012;Nikolouli et al., 2017), and transmission . For example, a study conducted on spiders infected with Wolbachia showed that the female ratio of the spiders was increased (which is beneficial for distribution) (Goodacre, Martin, Thomas, & Hewitt, 2006), and no significant difference was shown in the reproduction in H. graminicola (Yun et al., 2013).
Oxidative stress is a common consequence of the metabolic disturbance in animals and thus can be explained to the responses to elevated CO 2 (Matoo, Ivanina, Ullstad, Beniash, & Sokolova, 2013) and Wolbachia infection (Wong, Brownlie, & Johnson, 2015). Former studies indicated that elevated CO 2 increased the pCO 2 /pO 2 ratio (Pérez-López et al., 2009), which induced an oxidative stress response on invertebrates (Tomanek, Zuzow, Ivanina, Beniash, & Sokolova, 2011). Excess reactive oxygen species (ROS) has been generated by Wolbachia, resulting in the production of the oxidative stress (Brennan, Keddie, Braig, & Harris, 2008), which affect the lipids, nucleic acids, proteins, and antioxidants of the host (Fridell, Sánchez-Blanco, Silvia, & Helfand, 2005). This indicated that disturbances of oxidative stress balance may be a cocontribution of elevated CO 2 and Wolbachia; however, the interactive effects of these two factors are not totally illustrated in spiders, which need further investigation.
A large number of former studies have reported a single effect of either elevated CO 2 concentration or Wolbachia infection on invertebrate, but their interactive effect has been rarely studied. In addition, the ecological consequences of these interactions were not equal due to overlapping effects of the two factors (Murray, Ellsworth, Tissue, & Riegler, 2013). Former studies have proved that elevated CO 2 levels or Wolbachia infection separately has significant effects on spiders (Goodacre et al., 2006;Wang et al., 2016;. With increasing of CO 2 level and extending proportion of Wolbachia infection, H. graminicola would be certainly subjected to double stresses. At the same time, in view of the fact that both elevated CO 2 and Wolbachia infection provoke strong oxidative stress, it seems interesting and logical to hypothesis that there existed interaction between elevated CO 2 and Wolbachia. In the year 2015, atmosphere CO 2 concentration was 400 ppm, and it would be expected to rise to 970 ppm by the end of 21st K E Y W O R D S CO 2 level, endosymbionts, Hylyphantes graminicola, nutrition, peroxidase, survival rate century (Collins & Bell, 2004). According to the CO 2 levels (400 ppm, approximately concentration in the current and 800 ppm, double concentration) and Wolbachia status (Wolbachia-infected, W + and Wolbachia-uninfected, W − ), we divided H. graminicola into four treatments: W − 400 ppm, W − 800 ppm, W + 400 ppm, and W + 800 ppm. So, to elucidate the interactive effects of the two factors on H. graminicola, the total survival rate, the developmental duration, carapace width and length, body weight, sex ratio, net reproductive rate, nutrition content, and enzyme activities of H. graminicola were examined in this study.

| Spider collection and rearing
Fifty-eight female spiders were collected from wild corn (Zea mays) field located in Henan Province (112°49′E, 34°8′N), China, during September 2017. Most female adult spiders had exhibited copulatory behavior before capture in field. Female spiders were placed in cylindrical glass tubes (2 cm diameter, 6 cm high) with a layer of sponge (1.5-2 cm thick) moistened with water at the bottom, and bottleneck was plugged with cotton. These mother spiders were always kept under ambient CO 2 concentration in a chamber at 28°C, with 60%-70% relative humidity, and L14:D10 photoperiod (light on at 08:00 hr). Most spiders laid their first egg sac in a week after we brought them to the laboratory and hatched in another week.

The spiders were fed with adult Drosophila melanogaster without
Wolbachia infection and cultured under ambient CO 2 concentration.

| Establishment of infected and uninfected Wolbachia spiders
Female spiders were used to extract DNA for Wolbachia infection screening when 2-3 egg sacs were laid. If the mother spider and 2-s instar spiderlings from each egg sac were W + , then her offspring were confirmed as W + . The same method was used to test W − spiders. Wsp (Wolbachia surface protein) gene was used to detect the infection of Wolbachia in spiders (Rowley, Raven, & McGraw, 2004).
In 58 mother individuals, 25 spiders showed a Wolbachia infection (W + group) and 33 spiders had no a Wolbachia infection (W − group).
After egg sacs hatched, spiderlings were separated individually into cylindrical glass tubes (one glass tube for each spider) and perpetually deposited into their respective CO 2 concentration chambers until they died (ambient or high). Wolbachia infection was widely distributed in arthropods, and bioinformatics approach was essential for sequencing of Wolbachia strains which showed that most of Wolbachia belongs to subgroup A or B in most of arthropods (Pascar & Chandler, 2018). Wolbachia strain found in H. graminicola belongs to the supergroup B .
Four treatments of Wolbachia and spiderlings under different levels of CO 2 were used in the experiment. First treatment contained spiders without Wolbachia under 400 ppm of CO 2 (W − 400 ppm CO 2 ). In 2nd treatment, spiders were reared under 800 ppm of CO 2 without Wolbachia infection (W − 800 ppm CO 2 ). Spiderlings were infected with Wolbachia strain and reared under 400 ppm of CO 2 concentration in 3rd treatment, while in 4th treatment Wolbachiainfected spiders were reared under 800 ppm of CO 2 .
2.3 | Assessment of total survival rate, developmental duration, length and width of the carapace, body weight, sex ratio, and net reproductive rate (R 0 ) All spiderlings (W − 400 ppm treatment contains total 262 individuals, W − 800 ppm of CO 2 contains 257 individuals while W + 400 ppm CO 2 contains total 226 individuals and W + 800 ppm of CO 2 contains 220 ones) were selected randomly from 58 mother spiders. Survival rate is the ratio of number that spiderlings survive to maturity. Molts and reproduction were recorded during exuviation in the tube. The period of molts was used as a measure of developmental duration.
The data of survival rate and developmental duration were observed at 6:00 p.m. every day until spiders were dead. They were identified to sexual organ after spiderlings were mature. The number of male and female was recorded to calculate the sex ratio. Three days postmaturation, 15 adult females H. graminicola were randomly selected from each treatment and weighed to the nearest 0.01 mg using an electronic balance (FA1004 N type, HANGPING). Then, these female spiders from each treatment were killed in 75% alcohol to measure the body lengths (from the front edge of carapace to the end of abdomen), using an ocular micrometer under a microscope (DFC495 type, LEICA).
Net reproduction rate of spiders (R 0 ) is defined as the total number of mean number of descendants that a single spider can produce throughout its life time period and was calculated by the following formula: Where N t is egg number of beginning and N t+1 is egg number of next generations. We randomly selected 100 eggs (N t ) from each treatment to rear, females that survive to maturity mated with males under the same treatment, and counted the total number of eggs laid by females (N t+1 ). Sex ratio is basically number of males and females in a population.
Sex ratio = Number of males/number of females × 100

| Application of CO 2 concentration
Two concentrations of CO 2 , ambient (400 ppm) (WMO, 2016) and high (800 ppm), were set up using CO 2 artificial climate chambers (CC350TLHC type; Changzhou Okefenokee Instrument). The current ambient concentration of atmospheric CO 2 is 400 ppm, so our "highest" treatment of CO 2 was approximately double then the ambient level (Wang et al., 2016;Zuo et al., 2015).

| Determination of nutrient composition and enzyme activity
Three days after the spiderlings molted, more than 25 individuals were randomly selected from each treatment and pooled into one sample, and replicated thrice. These individuals were used for the determination of nutrient composition and enzyme activity with the help of specific test kits. All kits were purchased from the Jiancheng Bioengineering Institute: quantitative total protein (A045-2), total amino acid (A026), amylase activity (C016), and peroxidase activity (A084-1).
Bradford method was used for detection of proteins (Bradford, 1976).
Absorbance of the sample was recorded at 595 nm wavelength (Deng et al., 2013). The shelf life of this kit was 6 months. Storage temperature was 2-8°C. This kit measured 0.2-1.3 mg/ml of sample, and it measured even four times more accurately then other methods. The POD activity is measure of change in absorbance at 420 nm .
The amylase activity was measured at 650 nm of wavelength according to instructions on kit. Kit examination range was 0-200 μmol/ml.
After weighing, spiders were added to 0.9% saline solution at 1:9 fresh weight-to-volume ratio. Optical density (OD) values were measured using an ultraviolet-visible spectrophotometer (UV-6100,

Shanghai Precision Instrument) and ELISA (SpectraMax Series
Microplate Reader 190). Nutrients content and specific enzymes activities were calculated according to the kit instructions.

| Statistical analysis
The developmental duration was analyzed by Mann-Whitney U test.
Comparisons of sex ratio were done using chi-square tests within variant CO 2 concentration group or Wolbachia group. Comparisons of carapace width and length, body weight, net reproductive rate, nutrition content, and enzyme activity of H. graminicola were done using Student's t test within variant CO 2 concentration group or Wolbachia group. The data were tested for normal distribution and homogeneity of variance using K-S (Kolmogorov-Smirnov) test and Levene's test of equality of error variances, respectively. All interactive effects of CO 2 concentration and Wolbachia infection were analyzed using two-way ANOVA for significant comparisons.

| Total survival rate of H. graminicola
The total survival rate of H. graminicola in four treatments, W − 400 ppm (Wolbachia-uninfected with 400 ppm CO 2 ), W − 800 ppm

| Developmental duration of H. graminicola
The total developmental duration of male H. graminicola was shorter than that of female spiders, and no significant effects were observed under high CO 2 concentrations with Wolbachia-uninfected spiders (Mann-Whitney U test, female: p = 0.402; male: p = 0.690). However, in the Wolbachia-infected population of H. graminicola, a significantly shorter total developmental duration was observed (Mann-Whitney U test, female: p < 0.0001; male: p = 0.009). In addition, the developmental duration of second to fourth instar was significantly different between the two CO 2 concentrations (p < 0.05; Table 1). These observations suggest that Wolbachia infection might be the main factor for total developmental duration and thus promoted the growth rate of H. graminicola.

| Carapace size and body weight of H. graminicola
Carapace width of Wolbachia-uninfected spiders was significantly shorter when reared with elevated CO 2 concentration (Student's t test, t = 5.321, p < 0.0001) while, the carapace width of Wolbachia-infected spiders was significantly decreased under high CO 2 concentration (Student's t test, t = 4.574, p = 0.001). Compared with ambient CO 2 treatment, no obvious difference was observed in carapace length under high CO 2 concentration; however, carapace length was significantly decreased in Wolbachia-infected spiders (Student's t test, t = 2.949, p = 0.008; Table 2). The body weight

| Sex ratio and net reproductive rate (R 0 ) of H. graminicola
The female:male ratios of W − 400, W − 800, W + 400, and W + 800

| Nutrient composition and enzyme activities of H. graminicola
Elevated CO 2 concentration did not affect the amino acid content in  TA B L E 2 Mean (±SD; n = 15) of carapace width (mm), carapace length (mm), and body weight (mg) in Hylyphantes graminicola with four treatments species of spiders in the field of maize and cotton (Zhao, 1993).

| D ISCUSS I ON
The effects of ecological environment caused by the gradually rising atmosphere CO 2 concentration have attracted more and more attention. The survival rate of phytophagous insects varies with different species and host plant. Agrell, McDonald, and Lindroth (2000) reported that the survival rate of Orgyia leucostigma (Lepidoptera: Lymantriidae) was significantly declined under elevated CO 2 concentration. In contrast, Osbrink, Trumble, and Wagner (1987) showed nonsignificant differences under different CO 2 concentrations in Trichoplusia ni (Lepidoptera: Noctuidae). In this study, the total survival rate was not significant among the four treatments, and H. graminicola was not affected by the interaction of elevated CO 2 concentration and Wolbachia infection.
We speculated that only one generation of H. graminicola was reared and assessed under elevated CO 2 concentration; therefore, the effects on total survival rate might be limited. Furthermore, a nonsignificant relationship was found between the total survival rate and Wolbachia infection in H. graminicola. Yun et al. (2013) and Zhang, Yang, Zhu, and Hong (2018) assessed Wolbachia as endosymbiotic bacteria that induce beneficial effect in spiders' growth and development process; this beneficial association was helpful in regulation of biological traits in spiders. Our findings coincide with them.
The response of elevated CO 2 concentration in predatory insects is species-specific (Ge, Chen, Wu, & Sun, 2010). The elevated CO 2 concentration decreased the relative growth rate in cotton bollworm, H. armigera (Wu, Chen, & Ge, 2006), whereas the developmental duration was accelerated in Sitobion avenae (Homoptera: Aphididae) (Sun, Jing, & Ge, 2010). Similar results were found in different species in response to Wolbachia infection, whereby, different Wolbachia strains and host species exert different effects on fitness and growth. The developmental durations of prepupal stages in Wolbachia infection were significantly longer than uninfected Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae) (Yang et al., 2008). In Mediterranean fruit fly (Diptera: tephritidae), Wolbachia infection shortened the total developmental duration, but it prolonged the embryonic development (Sarakatsanou, Diamantidis, Papanastasiou, Bourtzis, & Papadopoulos, 2011). Our study indicated that the total developmental duration was significantly decreased after Wolbachia infection, which proved that the Wolbachia infection accelerated the growth rate, and the total developmental duration did not significantly discriminate between the two CO 2 concentrations in Wolbachia noninfected spiders. This phenomenon might be explained by the interaction of elevated CO 2 and Wolbachia infection responses, but the definite molecular mechanism is still unclear.
Carapace width and weight are important tools for evaluating the development of spiders (Gonzaga & Vasconcellos-Neto, 2002;Hagstrum, 1971;Jakob, Marshall, & Uetz, 1996). Our results showed that the carapace width was decreased significantly when exposed to high CO 2 concentration, but carapace length and weight showed a nonsignificant effect. However, the effects on carapace size were similar to P. astrigera . We speculated that H. graminicola can prey like a wandering spider (Zhao, 1993), so elevated CO 2 concentration might affect respiratory rate (Miyashita, 1969) Eresidae) (Avilés & Maddison, 1991;Avilés, Varas, & Dyreson, 1999), while in solitary spiders, like Pityohyphantes phrygianus (Araneae: Linyphiidae), there was no direct relationship between Wolbachia infection and sex ratio variation (Gunnarsson, Goodacre, & Hewitt, 2009). Our data indicated that in H. graminicola, as a solitary spider, like P. phrygianus, the sex ratio was not affected by Wolbachia.
Moreover, a relationship between sex ratio and elevated CO 2 concentration has not been found. Accordingly, there was no significant interaction of elevated CO 2 concentration and Wolbachia infection in H. graminicola.
The fertility of different insect species under elevated CO 2 concentration varies (Chen, Wu, Ge, Parajulee, & Shrestha, 2005). Zuo et al. (2015) indicated that the total number of eggs was significantly decreased in P. astrigera under elevated CO 2 concentration. However, the number of eggs laid by H. armigera did not differ between ambient and high CO 2 concentrations (Chen et al., 2005).  (Schuler et al., 2018).
Elevated CO 2 concentration affect the nutrient composition and enzyme activity in P. astrigera and increase total protein content but peroxidase activity was not altered (Zhou et al., 2015). Unlike P. astrigera, the amylase activity in A. labyrinthica was higher and the peroxidase activity did not differ noticeably. These observations show that elevated CO 2 concentration has a species-specific impact on spiders. Molecular mechanism for the symbiotic relationship between Wolbachia and their hosts has not been clearly expressed, but the changes in enzymatic activity of insect hosts harboring Wolbachia have been reported (Henrichfreise et al., 2009;Melnikow et al., 2013). In our research, when spiders were infected with Wolbachia and reared under elevated CO 2 concentration, the total protein, amylase activity, and peroxidase activity have been increased significantly. Amino acid content differed slightly but was not significantly increased under elevated CO 2 concentration, though it clearly increased when using Wolbachia-infected spiders. Total protein and amino acid content are indispensable for nutrition in insects, while amylase is an important hydrolase, and together they supply energy for survival and reproduction (Kong, Huang, Liu, & Liu, 2007;Sun, Jing, & Ge, 2009;Sun et al., 2010), thus these factors seem to be beneficial to H. graminicola. Peroxidase, an important protective enzyme in insects (Dubovskiy et al., 2008), catalyzes the decomposition of H 2 O 2 , which is required to maintain balance under conditions of oxidative stress (Ahmad & Pardini, 1990). Significantly, interactions of the two factors (elevated CO 2 concentration and Wolbachia infection) on total protein, amino acid content, amylase activity, and peroxidase activity were found in H. graminicola and synergistically increased the nutrition content and enzyme activities.
In summary, in H. graminicola, an elevated CO 2 concentration and Wolbachia infection were proved to be beneficial as it increases the protein contents and activity of some enzymes like peroxidase, amylase, and amino acids; thus, it aids in the development of spiders under dynamic environment but the impact on the reproduction of the spider was limited. Spiders are bioindicator of environmental pollution. Elevated level of CO 2 is major threat to invertebrates existence and survival so rearing of spiders under stress condition (elevated CO 2 ) with endosymbiotic bacteria Wolbachia might be helpful for understanding the complexity and adaptability of the H. graminicola population, and it will be used as biological control of other pests in the field so in this way ecofriendly strategy should be devised that will protect the environment from harmful effect of chemical pesticides and developmental duration of spider was TA B L E 3 Mean (±SD; n = 3) of total protein, amino acid content, peroxidase activity, and amylase activity in Hylyphantes graminicola with four treatments Amylase (U/mg protein) 0.798 ± 0.681aA 0.808 ± 0.355bA 1.093 ± 0.056aB 2.010 ± 0.301bB Note: Different lowercase letters indicate significant difference between CO 2 treatments within Wolbachia-infected and Wolbachia-uninfected spider. Different uppercase letters indicate significant differences between Wolbachia-infected and Wolbachia-uninfected spider within CO 2 concentration (Student's t test, p < 0.05).
accelerated. The elevated CO 2 and Wolbachia infection enhance digestive enzymes and detoxification enzymes, it seems interesting and logical to hypothesis that there existed interaction between elevated CO 2 and Wolbachia.

ACK N OWLED G M ENTS
This study was supported by the National Natural Science Fund of China (31672317)

CO N FLI C T O F I NTE R E S T
All of the authors declare that they have no conflict of interest in the publication.

AUTH O R CO NTR I B UTI O N S
Yu Peng and Jian Chen designed the experiments. Qichen Su, Xia Wang, Naila Ilyas, Fan Zhang, and Yu Peng conducted the experiments and data analysis. Qichen Su, Xia Wang, Yueli Yun, and Yu Peng wrote the manuscript.

DATA ACCE SS I B I LIT Y
All essential data are available in the text.