Reproductive isolation due to prezygotic isolation and postzygotic cytoplasmic incompatibility in parasitoid wasps

Abstract The reproductive barriers that prevent gene flow between closely related species are a major topic in evolutionary research. Insect clades with parasitoid lifestyle are among the most species‐rich insects and new species are constantly described, indicating that speciation occurs frequently in this group. However, there are only very few studies on speciation in parasitoids. We studied reproductive barriers in two lineages of Lariophagus distinguendus (Chalcidoidea: Hymenoptera), a parasitoid wasp of pest beetle larvae that occur in human environments. One of the two lineages occurs in households preferably attacking larvae of the drugstore beetle Stegobium paniceum (“DB‐lineage”), the other in grain stores with larvae of the granary weevil Sitophilus granarius as main host (“GW‐lineage”). Between two populations of the DB‐lineage, we identified slight sexual isolation as intraspecific barrier. Between populations from both lineages, we found almost complete sexual isolation caused by female mate choice, and postzygotic isolation, which is partially caused by cytoplasmic incompatibility induced by so far undescribed endosymbionts which are not Wolbachia or Cardinium. Because separation between the two lineages is almost complete, they should be considered as separate species according to the biological species concept. This demonstrates that cryptic species within parasitoid Hymenoptera also occur in Central Europe in close contact to humans.

The third important component of speciation, intrinsic postzygotic isolation is mainly due to incompatibilities between genomes of divergent populations, termed Dobzhansky-Muller incompatibilities (DM or DMI) or Bateson-Dobzhansky-Muller incompatibilities (BDM) which lead to infertility, inviability, or other negative fitness effects in hybrids (Coyne & Orr, 2004;Lowry, Modliszewski, Wright, Wu, & Willis, 2008;Presgraves, 2010). A special case of postzygotic isolation is cytoplasmic incompatibility (CI) induced by endosymbiotic bacteria (Brucker & Bordenstein, 2012). These bacteria prevent the production of offspring when infected males are mated to uninfected females, by causing defects in the first mitotic division of the zygote (Serbus, Casper-Lindley, Landmann, & Sullivan, 2008;Tram, Fredrick, Werren, & Sullivan, 2006). These endosymbionts are transmitted vertically to the offspring only by the females and have no effect when females and males are both infected, or when only females are infected. Therefore, CI increases the frequency of the inducing endosymbionts in the host population, by supporting infected females at the expense of noninfected females (Werren, 1997;Werren, Baldo, & Clark, 2008). So far, only two bacteria, Wolbachia and Cardinium, have been described to induce CI (White, 2011).
In the present study, we addressed reproductive barriers in the chalcidoid parasitoid Lariophagus distinguendus (Förster; Figure 1) with the aim to better understand speciation in parasitoid Hymenoptera.
L. distinguendus is attacking beetle larvae living in grains, seeds, or cocoons (Niedermayer & Steidle, 2013;Steidle & Schöller, 1997). For many decades, its general biology, ecology, and use for the biological control of stored product pests have been extensively studied by many authors (reviewed by Niedermayer, Pollmann, & Steidle, 2016). König et al. (2015) reported on two distinct lineages of L. distinguendus which are ecologically separated. One prefers drugstore beetles Stegobium paniceum (L.) (Anobiidae) as hosts and is found in households on beetle-infested products ("DB-lineage"), while the other is mostly found in granaries attacking the granary weevil Sitophilus granarius (L.) (Dryophthoridae: Curculionoidea) ("GW-lineage"). Because recent findings demonstrate that the DB-lineage is ancestral (König et al., 2019), a host switch must have occurred, from drugstore beetles to granary weevils (König et al., 2015(König et al., , 2019. Interestingly, the population GWpfo, which belongs to the GW-lineage, maintained this ancestral preference for drugstore beetles but was collected from granary weevils in a grain store. Molecular analyses revealed considerable genetic differences between the two lineages (König et al., 2015), which also have different numbers of chromosomes (König et al., 2019). However, reproductive barriers between the lineages have not been studied and it is unclear if they represent conspecific ecotypes or separate species according to the biological species concept (Mayr, 1969).
We studied reproductive barriers in five populations, two from the DB-lineage and three from the GW-lineage, to distinguish between barriers which occur early during separation, that is within the lineages, and barriers which occur later, that is between the lineages (Coyne & Orr, 2004;Jennings, Snook, & Hoikkala, 2014). Potential sexual prezygotic isolation was addressed by studying courtship and mating behavior. Postzygotic isolation, that is, the absence of fertilization, was studied by counting the number of hybrid F 1 female offspring. Only the number of F1 female offspring was used as a parameter, because males are haploid in Hymenoptera and emerge from eggs without fertilization. To address the role of endosymbionts causing CI as isolating barrier, we analyzed reproductive isolation between a single focal pair of populations (one of each lineage) after treatment with and without antibiotics.
The study revealed that both lineages are almost completely isolated and should be considered as separate species. We found one case of sexual isolation between two populations of the same species, which occur on the same host, indicating that speciation can be initiated by sexual isolation due to the divergence of sexual pheromones. Speciation is completed in this taxon by ecological separation, strong sexual isolation and by postzygotic barriers, including endosymbiont induced cytoplasmic incompatibility.

| Insects
The experiments were performed with wasps from five populations of two lineages of L. distinguendus collected by us or sent to us by conditions is about 20 days in females and 19 days in males (Ryoo, Hong, & Yoo, 1991). Wasps of DBrav and DBstu were reared on 3rd to 4th instar larvae of the drugstore beetle S. paniceum infesting koi pellets (Hikari Friend, Kamihata Fish Industry Group, Kyorin Corporation, Japan) in glass jars (diameter 12 cm, 16 cm height) with a ventilated lid. Wasps of GWpfo, GWslo, and GWsat were reared on 3rd to 4th instar larvae of the granary weevil S. granarius infesting wheat grain (Triticum aestivum L.; cultivar: Batis; Saaten-Union GmbH, Hannover, Germany) in Petri dishes. To exclude the potential impact of different rearing conditions in our experiments on the occurrence and number of F 1 female hybrid offspring with DBrav and DBstu, we used wheat grains instead of KOI-pellets as rearing substrate for the drugstore beetles. Apart from that, rearing conditions were as described above with the exception that 1 ml water was used to moisten grains in order to facilitate oviposition of female wasps.
To rear drugstore beetles, about 1 g of newly emerged unsexed adult drugstore beetles (about 700 beetles) was placed in a Petri dish on 80 g food pellets for koi fish or on 40 g wheat grain moistened with 1 ml water for oviposition and left there until they died. After six weeks, L. distinguendus were placed on the infested pellets or wheat grains, depending on the experiment. To rear granary weevils, unsexed adult granary weevils (2.7 g, about 600 beetles) were placed in jars (diameter 12 cm, 16 cm height) with a ventilated lid on 40 g of moistened wheat grain (40 ml H 2 O on 1 kg grain) for oviposition. After one week, adult beetles were removed. Beetle cultures were kept at 27°C and 65% r.h. and natural daylight conditions.

| Behavioral studies
To study sexual isolation, experiments were performed similar to well-established mating experiments with Nasonia as no-choice experiments with single females and males (Giesbers et al., 2013).
Individuals from all five populations in all cross combinations were tested under standardized conditions (23°C and 50%-60% r.h.). To ensure that females were unmated, wasps were separated directly after emergence from the cultures and kept in small groups of up to 10 individuals of the same sex in Petri dishes on a moist filter paper.
After 48 hr, one female and one male wasp were placed in a mating arena consisting of a glass Petri dish (diameter 14 mm) closed with a glass plate (30 mm × 30 mm). Mating behavior in L. distinguendus consists of courtship behaviors "wing fanning" and "antennal stroking" by the males, "receptivity signal" by the female (lowering the head, folding down the antennae and exposing the genitalia), and finally copulation (Ruther, Homann, & Steidle, 2000). These behaviors were observed with a stereo-microscope for a maximum observation time of 20 min and recorded using "The Observer v. 5.0" (Noldus). According to our experience, most pairs with females and males from the same population mate during this time. However, as demonstrated by the occurrence of female offspring (see below) in couples were no mating has been observed, some mating between females and males from different populations must have occurred after this observation time. Twenty pairs were studied per combination.

| Hybrid F 1 females
This experiment aimed to study reduced fertilization after mating of individuals from different populations, and/or reduced viability of hybrid F 1 females. Each pair from the previous behavioral studies was placed in a small Petri dish with 20 wheat grains infested by 3rd and 4th instar larvae of either drugstore beetles (when females were from populations DBrav or DBstu) or granary weevils (when females were from populations GWpfo, GWslo, or GWsat). This enabled mating for those pairs, which did not mate in the behavioral experiment, as well as oviposition by females on their preferred hosts. After 48 hr, the adult wasps were discarded. After 30 days, the number and sex of emerging F 1 offspring were registered. To study reduced fertilization as barrier between different populations, we used the occurrence of F 1 offspring ( Figure S1). To study reduced viability of hybrid F 1 females, we used the mean number of female F 1 offspring from each combination. Thereby, we based our analysis only on those pairs, which produced female offspring to exclude the influence of missing copulations.

| Role of CI as postzygotic barrier
To address the role of endosymbionts causing CI as isolating barrier, we analyzed reproductive isolation between a single focal pair of populations (DBrav and GWpfo, i.e., one of each lineage) after treatment with and without antibiotics. To establish endosymbiont free lines, freshly emerged wasps were mated and then placed into a Petri dish with a filter paper soaked with a tetracycline-sugar solution (1 mg/ml tetracycline in 10% sucrose; Breeuwer & Werren, 1993) for 24 hr. Then, wasps were placed on their breeding host as described above. This procedure was repeated for seven generations. For the experiments, females and males of the F29 to F42 generation after termination of antibiotic treatment were used. The absence of endosymbionts in these wasps was verified by PCR (Table 3).
For the experiments, wasps were collected immediately after emergence to avoid mating and kept in same-sex groups as described above. One virgin female and one virgin male were placed together on either 5 g pellets infested by larvae of the drugstore beetle (when females were from DBrav) or 10 g wheat grains infested by larvae of the granary weevil (when females were from GWpfo) and kept under constant conditions of 26°C and 45% r.h. at cycle of 16L:8D hours for 16 days. The number and sex of emerging wasps were registered after 30 days.

| Detection of CI-inducing endosymbionts
Ethanol preserved wasps were washed with sterile double distilled water (ddW) and grinded thoroughly in 10 μl of lysis buffer (mixture: 5 μl Tris 1 M, 1 μl EDTA, 5 μl Igepal, 50 μl Proteinase K, 939 μl ddW) in a Petri dish covered with aluminum foil and parafilm to extract their DNA. Additional lysis buffer (30 μl) was added and the whole fluid was pulled up and down with a pipette once. The lysed wasp was then transferred to a 1.5 ml tube and incubated for 15 min at 65°C and 10 min at 95°C. DNA concentration and nucleic acid/protein ratio (260/280 nm) of the conducted DNA extractions were measured using a Thermo Scientific NanoDrop 2000 spectrophotometer. Samples with concentrations between 40 and 500 ng/μl and ratios above 1.8 were used for further analysis.
Samples with lower concentration or poor quality were discarded.
Five to ten specimens of each of the five wasp populations and different generations of antibiotic-treated strains of DBrav and GWpfo were tested for Wolbachia and Cardinium using polymerase chain reaction (PCR) amplification with several specific and with one universal bacterial 16SrRNA primer (Table 1). PCR conditions are given in Table 2. PCR amplifications were performed in 13 μl of reaction buffer containing 6.5 μl Promega GoTaq ® Green Master Mix 2X, 2 μl template DNA, 0.5 μl 10 μM of each primer, and 3.5 μl ddW using a Biometra professional Basic Thermocycler. Sterile, double distilled water was used as a negative control. Positive controls were provided from Nesidiocoris tenuis and Culicoides spp. Resulting PCR products were loaded on a 1.5% agarose gel stained with ethidium bromide for visualization under UV-light. Molecular weight size markers used were Norgen LowRanger 100 bp DNA Ladder and New England BioLabs Inc. Quick-Load ® Purple 2-Log DNA Ladder.

| Calculation of isolation indices
Reproductive isolation (RI) is the strength of any pre-or postzygotic barrier and represents an estimate how much gene flow is reduced by this barrier (Coyne & Orr, 2004). Based on Sobel and Chen (2014) RI varies from −1 (the barrier allows for gene flow only between heterospecifics) over 0 (gene flow is random) to 1 (only gene flow between conspecifics). RI was calculated according to the following formula: H refers to events, which enable gene flow between heterospecifics (i.e., frequency of mating between heterospecifics or the number of offspring after mating of heterospecifics), while C refers to events, which enable conspecific gene flow.
RI for habitat isolation and immigrant inviability was calculated according to Nosil, Vines, and Funk (2005) (Ramsey, Bradshaw, & Schemske, 2003). It was calculated as follows: Total isolation (T) was calculated using the following formula:

| Estimation of evolutionary divergence over sequence pairs between groups
The number of base differences per site from averaging over all sequence pairs between groups was estimated using the partial mitochondrial cytochrome oxidase I (COI) sequences with a total of 679 positions in the final dataset. This analysis involved 10 nucleotide sequences (GenBank Accession numbers: KJ867375-KJ867378 and KJ867383-KJ867388) from König et al. (2015). Evolutionary analyses were conducted in MEGA X (Kumar, Stecher, Li, Knyaz, & Tamura, 2018). Additional sequences from Nasonia (EU746609-EU746612) from Oliveira, Raychoudhury, Lavrov, and Werren (2008) were used to compare evolutionary divergence.

| Statistical analysis
Data from behavioral studies and data on the occurrence of F1  (Hothorn, Bretz, & Westfall, 2008).
F I G U R E 3 Mean number of F 1 female offspring (+ confidence intervals) from pairs consisting of females and males from five populations of Lariophagus distinguendus. Blue bars: female and male from the same population and the same lineage; yellow bars: females and males from different populations, but from the same lineage; red bars: females and males from different populations and different lineages; x = no F 1 female offspring available for testing. The number of pairs tested for each combination is given in brackets. Differences in offspring numbers between pairs from the same population, pairs from different populations, but from the same lineage and pairs from different lineages were analyzed using a linear mixed model with female population as random factor followed by ANOVA and Tukey tests

| Behavioral studies
The mating experiments revealed that males reacted equally well to females of all populations from both lineages, while females were much more restricted in their response. All experiments were grouped according to the female population. For every group, we compared the occurrence of each behavior. Experiments with males and females from the same population were used as control and compared to the other combinations. "Wing fanning" toward females and "antennal stroking" on the females was performed by males in almost all replicates of the intralineage and interlineage combinations. There were no significant differences (Figure 2; Tables S1-S3). In contrast, the receptivity signal by females and subsequent copulation were observed almost exclusively in the intralineage combinations (blue and yellow bars in Figure 2) and only rarely in the interlineage combinations (red bars in Figure 2). For all tested groups, there were significant differences between intralineage and interlineage combinations (Figure 2; Tables S4 and S5).
Interestingly, a significant (about 50%) decrease in the occurrence of receptivity signal and copulations was also observed between the two populations DBrav and DBstu, which belong to the same lineage and occurred on the same host.

| Postzygotic barriers causing hybrid female inviability
In Figure 3 and Figure S1 experiments are grouped according to the female population as in the behavioral experiments. While most pairs from the nonhybrid control crosses with females and males from the same population (number of replicates for blue bars in Figure 3; blue bars in Figure S1; Table S6) or from populations of the same lineage (number of replicates for yellow bars in Figure 3; yellow bars in Figure S1; Table S6) produced female offspring, this was not the case with pairs from the interlineage combinations (number of replicates for red bars in Figure 3; red bars in Figure S1; Table S6).
Thereby, female offspring occurred in several pairs that did not mate in the behavioral experiments. Obviously, mating must have taken place in these pairs in the Petri dish with hosts after the behavioral experiment was finished.
It is unclear, if missing female offspring was caused by the absence of copulations with foreign males, a prezygotic barrier, by missing fertilization as a result of postmating-prezygotic isolation, or by postzygotic isolation (Coyne & Orr, 2004). Therefore, to analyze the number of F1-female offspring that were produced by nonhybrid and hybrid parental pairs, we studied only the pairs with female offspring, as indication that copulation and fertilization has taken place. Due to the low number of these pairs in the combinations between lineages, data were pooled for statistical analysis. All data from control combinations (female and male from the same population, blue bars in Figure 3; n = 95), the hybrid intralineage combinations (female and male from different population but from the same lineage, yellow bars in Figure 3; n = 151), and the interlineage combinations (female and male from different lineages; red bars in Figure 3; n = 22) were compared using a linear mixed model with female population as random factor followed by ANOVA and the Tukey tests. This revealed a significant difference within the three groups (ANOVA for groups: χ 2 = 72.00, df = 2, p ≤ .001; Figure 3).

Single comparisons showed that hybrid crosses between lineages
(see red bars in Figure 3) produced significantly less female offspring than hybrid crosses within a lineage (yellow bars in Figure 3; Tukey test: z = 8.412, p ≤ .001) and nonhybrid controls (blue bars in Figure 3; Tukey test: t = -6.463, p ≤ .001). Hybrid intralineage crosses produced more hybrid F 1 female offspring than nonhybrid controls (Tukey test: z = 2.984, p ≤ .0083).
Cytoplasmic incompatibility (CI) was studied with wasps from the DBrav and the GWpfo populations, which were untreated or treated with antibiotic. PCR-analyses revealed that wasps treated with antibiotic were free of endosymbionts (Table 3) Table S7). When GWpfo-females were mated with endosymbiont free DBrav males, they produced significantly more   Sobel and Chen (2014), and total isolation using the absolute contribution of each barrier according to Ramsey et al. (2003) Cross Note: Habitat isolation and immigrant viability are based on the data on host preference and fecundity from König et al. (2015).
infected with CI-inducing bacteria. Diagnostic PCR for Wolbachia and Cardinium revealed that the DBrav population, as well as the population DBstu from the same lineage, and antibiotic-treated wasps were not infected with either bacteria, while GWpfo and the two other populations from this lineage (GWslo, GWsat) were infected with Wolbachia (all tested wasps were positive). Positive controls and negative controls resulted as expected (Data not shown).
Thus, CI-inducing bacteria in the DB-lineage must be different from Wolbachia or Cardinium. Data from the crosses DBrav♀ × GWpfo♂ did not reveal F 1 female offspring in all treatment combinations, indicating that additional barriers were involved, such as sexual isolation (see Section 4).

| Isolation indices
Isolation indices (RI) for the different barriers revealed only slight isolation due to sexual isolation between DBrav × DBstu within the DB-lineage, and no isolation within the GW-lineage (Table 4). Sexual isolation between the lineages was almost complete. Isolation due to habitat choice and immigrant inviability (calculated using data from König et al., 2015) only occurred between the two lineages and ranged from absent to strong. Postzygotic isolation due to a reduced number of F 1 females from hybrid pairs varied from weak to absolute. Total isolation based on all barriers together was complete or almost complete between the lineages.

| D ISCUSS I ON
We comparatively studied pre-and postzygotic reproductive barriers in five populations of the parasitoid wasp L. distinguendus, which belong to two distinct lineages and might well be different species. In the following, we will discuss ecological, sexual and intrinsic postzygotic barriers that have been identified between the populations.

| Ecological isolation
Because the two lineages of L. distinguendus have been found on two different host species (DB-lineage on larvae of drugstore beetles, GW-lineage on larvae of granary weevils) and in different environments (households vs. granaries, respectively), they are ecologically isolated. We calculated isolation indices for habitat isolation and immigrant viability as suggested by Nosil et al. (2005) based on the host recognition behavior on grains infested by the two different host and the offspring number on the two hosts in an earlier study (König et al., 2015). Isolation between the two lineages is not absolute, but varies between the different combinations of populations from zero (0.09 for habitat isolation and -0.08 for isolation due to immigrant inviability), to strong (0.86 and 0.76, respectively). This is for two reasons. First, populations of the GW-lineage have a high fecundity on both hosts. Second, the population GWpfo has an innate preference for drugstore beetles, although it was found on granary weevils as hosts and genetically belongs to the GW-lineage (König et al., 2015). Therefore, we assumed that during the process of divergence a host switch occurred from drugstore beetles to granary weevils, and that the GWpfo population has retained an ancestral innate preference for drugstore beetles which is also found in the populations of the DB-lineage (König et al., 2015). The assumption that the DB-lineage is ancestral was supported by our recent karyological study (König et al., 2019).

| Sexual isolation
Our behavioral studies revealed that females signaled receptivity and mated only when courted by males from the same lineage. In addition, we observed a significant 50% decrease in reaction of females from the DB-lineage to males from the other population of the same lineage. The absence of a receptivity signal by the female upon courtship by heterospecific males was most likely due to the male oral gland pheromone, which is applied onto the female antenna during courtship (König et al., 2015). Sealing the male mandibles with glue to block the pheromone release resulted in the same behavior observed in our experiments, that is missing female receptivity (König et al., 2015). Obviously, the male oral pheromone differs between the two species and also between the two populations within the DB-species. Isolation indices for sexual isolation based on these behavioral data are close to zero between the three populations from the GW-lineage, at around 0.3 between the two populations from the DB-lineage, and close to 1 between populations from different lineages. Thus, female mate choice results in slight sexual isolation between the two populations of the DB-lineage and in almost complete sexual isolation between the two lineages.
The isolation between the two DB-populations is remarkable, because both prefer the same host (König et al., 2015), there were no molecular differences between the two populations based on mitochondrial and nuclear markers (König et al., 2015), and no other barriers are known so far. This demonstrates that the separation of populations during speciation in L. distinguendus can start with sexual isolation based on female mate choice. However, the fact that no sexual isolation was detected between populations of the GW-lineage indicates that this finding cannot be generalized for our study system.
In contrast to the response of the females, almost all males reacted to heterospecific females by courtship behavior, despite the fact that female cuticular hydrocarbons that stimulate the male response (Ruther et al., 2000) differ between the two species (Kühbandner, Hacker, Niedermayer, Steidle, & Ruther, 2012). This sex-specific difference in the reaction to varying male and female pheromones is in agreement with the asymmetric tracking hypothesis (Phelan, 1992;Wyatt, 2014). It states that due to differences in paternal investment and therefore a different number of potential offspring, males are expected to react to a larger variation in sexual signals as compared to the females, which should be more choosy to avoid offspring with unsuitable males with potentially reduced fitness (Phelan, 1992(Phelan, , 1997b(Phelan, , 1997a.

| Postzygotic isolation and CI
Hybrid female inviability as intrinsic postzygotic barrier was studied based on the occurrence and the mean number of F 1 female offspring in mixed pairs from different populations and lineages. Only few mixed pairs from different lineages produced female offspring ( Figure S1) and if so the female offspring number was significantly reduced. In contrast, mixed pairs of the same lineage had even more female offspring than control pairs, most likely due to heterosis (Benvenuto et al., 2012). Isolation indices were almost 0 between populations from the same lineage and ranged from 0.19 to 1 between populations from different lineages.
Potential reasons for the reduction in female offspring from pairs of different lineages are (a) sexual isolation, (b) gametic isolation, a postmating-prezygotic barrier, (c) postzygotic barriers like CI induced by endosymbionts, (d) chromosomal rearrangements, or (e) genic incompatibilities (Coyne & Orr, 2004 which often lays its single eggs in the powdery feces of the host where they cannot be retrieved for further examination. We studied the presence of bacterial induced CI between DBrav (representative for the DB-lineage) and GWpfo (for the GW-lineage). These studies revealed that DBrav, but not GWpfo, is infected with bacteria, which induce CI between DBrav and GWpfo. This explains part of the observed postzygotic isolation between the two lineages. Remarkably, we were not able to find any evidence for the presence of Wolbachia or Cardinium in both populations of the DB-lineage. These are the only two bacteria for which CI has been described (White, 2011).
Obviously, the DB-lineage is carrying an hitherto unknown, CI-inducing bacterium. We are currently working on its identification.
CI cannot explain the reduction of female offspring in pairs consisting of GWpfo-males and DBrav-females because males from GWpfo do not cause CI, regardless whether they had been treated with antibiotics or not. Based on our findings on sexual isolation, we consider it most likely that this reduction is due to female mate choice, that is the rejection of GWpfo-males by DBrav-females.
However, at least parts of the effect might be also caused by the fact that the two L. distinguendus species have different chromosome numbers (Gokhman, 2015), or by genetic incompatibilities.
More studies are required to answer this question.
Thus, the two Lariophagus lineages must be considered true, separate species according to the biological species concept (Mayr, 1969) representing another example of cryptic diversity within parasitoid Hymenoptera. This supports the idea that within the Hymenoptera a large number of species are still undescribed, even in Europe with a seemingly well studied fauna (Aguiar et al., 2013).

| CON CLUS IONS
Our study revealed that L. distinguendus, a parasitoid which occurs in human environments and has been studied for a long time (Niedermayer et al., 2016), consists of at least two different species.
These species are reproductively separated by (a) ecological isolation, (b) almost absolute sexual isolation due to female mate choice, (c) postzygotic isolation due to CI caused by a yet undescribed endosymbiotic bacterium, (d) and other, hitherto unknown barriers.
Interestingly, slight sexual isolation, based on the divergence of sexual pheromones was found between two populations of one species, which are not ecologically separated. This indicates that sexual isolation has the potential to initiate speciation. However, because this finding is restricted to only one conspecific population pair of L. distinguendus, it cannot be generalized. Therefore it is unclear, if speciation in L. distinguendus started with prezygotic isolation based on ecological or sexual selection, or by postzygotic isolation due to CI. More studies with closely related populations of L. distinguendus are required to answer this question.

CO N FLI C T O F I NTE R E S T
None declared.