Predicting the potential global distribution of Ageratina adenophora under current and future climate change scenarios

Abstract Aim Invasive alien species (IAS) threaten ecosystems and humans worldwide, and future climate change may accelerate the expansion of IAS. Predicting the suitable areas of IAS can prevent their further expansion. Ageratina adenophora is an invasive weed over 30 countries in tropical and subtropical regions. However, the potential suitable areas of A. adenophora remain unclear along with its response to climate change. This study explored and mapped the current and future potential suitable areas of Ageratina adenophora. Location Global. Taxa Asteraceae A. adenophora (Spreng.) R.M.King & H.Rob. Commonly known as Crofton weed. Methods Based on A. adenophora occurrence data and climate data, we predicted its suitable areas of this weed under current and future (four RCPs in 2050 and 2070) by MaxEnt model. We used ArcGIS 10.4 to explore the potential suitable area distribution characteristics of this weed and the “ecospat” package in R to analyze its altitudinal distribution changes. Results The area under the curve (AUC) value (>0.9) and true skill statistics (TSS) value (>0.8) indicated excelled model performance. Among environment factors, mean temperature of coldest quarter contributed most to the model. Globally, the suitable areas for A. adenophora invasion decreased under climate change scenarios, although regional increases were observed, including in six biodiversity hotspot regions. The potential suitable areas of A. adenophora under climate change would expand in regions with higher elevation (3,000–3,500 m). Main conclusions Mean temperature of coldest quarter was the most important variable influencing the potential suitable area of A. Adenophora. Under the background of a warming climate, the potential suitable area of A. adenophora will shrink globally but increase in six biodiversity hotspot regions. The potential suitable area of A. adenophora would expand at higher elevation (3,000–3,500 m) under climate change. Mountain ecosystems are of special concern as they are rich in biodiversity and sensitive to climate change, and increasing human activities provide more opportunities for IAS invasion.


| INTRODUC TI ON
Invasive alien species (IAS) are recognized as one of the main drivers of global environmental change (Simberloff et al., 2013). IAS lead to biodiversity loss (Bellard et al., 2016;Clavero & Garciaberthou, 2005), affect the ecosystem function and services (Vilà et al., 2010), and cause economic losses (Diagne et al., 2020;Ekesi et al., 2016;Paini et al., 2016). Climate change and anthropogenic activities, such as international trade, tourism, and road network expansion, play important roles in the expansion of IAS (Bertelsmeier et al., 2015(Bertelsmeier et al., , 2017Wan & Wang, 2018) by providing opportunities for IAS to spread and accelerating IAS expansion . IAS are commonly believed to be closely related to climate change (Alexander et al., 2016;Merow et al., 2017;Rodríguez-Merino et al., 2018;Zhao et al., 2013), and Richardson and Rejmánek (2011) predicted that climate change will accelerate IAS invasion. However, the relationship between IAS and climate change remains unclear since their interaction is quite complex (Merow et al., 2017). Exploring the spatial patterns of potentially suitable areas for IAS at present and in future is an effective way to prevent the further expansion of IAS (Fournier et al., 2019;Kaiser & Burnett, 2010;Keller et al., 2007).
Several recent studies have analyzed the potential changes in IAS distributions under multiple climate change scenarios at regional and global scales. Species distribution models (SDMs) have been widely applied in the early detection IAS (Ahmad et al., 2019;Padalia et al., 2014;Rodríguez-Merino et al., 2018;Srivastava et al., 2018;Zhang et al., 2015;Zhao et al., 2013) by mapping potential IAS distribution and quantifying the relationships between IAS and environmental factors based on occurrence-only data and species habitat conditions (e.g., climate, soil conditions, and terrain).
Ageratina adenophora (Sprengel) R. King and H. Robinson (synonym: Eupatorium adenophorum Sprengel), also known as Crofton weed, is regarded as one of the most serious invasive species in Asia, Africa, and Oceania (Tang et al., 2019). A. adenophora is native to Mexico (Qiang, 1998) and was introduced as an ornamental plant to other regions, including the United Kingdom (Auld & Martin, 1975), Hawaii (Muniappan et al., 2009), Australia (Auld, 1969), India (Bhatt et al., 2012;Poudel et al., 2019), South Africa (Kluge, 1991), Nepal (Tiwari, 2005), and Italy (Del Guacchio, 2013). A. adenophora is classified as one of the worst IAS in China (Yan et al., 2001;Zhang et al., 2008). The ecological attributes of A. adenophora contribute to its invasive ability. First, it possesses strong sexual and asexual reproductive capacity (Feng, 2008). According to Parsons (1992), one ramet can produce up to 10,000 seeds per season, including some 15% to 30% viable seeds. The seeds are capable of discontinuous germination, which prolongs their viability (Shen et al., 2011). Furthermore, the seeds are tiny scale, facilitating their spread by wind and water; the seeds of A. adenophora can disperse over both short and long distances (Wang et al., 2011;Zhang et al., 2008). A. adenophora also possesses a strong allelopathic effect, allowing it to compete with native species (Heather et al., 2011;Zhong et al., 2007). Research has shown that A. adenophora can alter the soil microbial community, which may inhibit native species and benefited its own growth Xu et al., 2012). In combination with the above traits, the high-stress tolerance Rivera et al., 2017) and high morphological plasticity (Shen, 2019;Zhao et al., 2013) of A. adenophora make it an "ideal" weed (Baker et al., 1965). The invasion of A. adenophora has significantly influenced the native biodiversity and resulted in enormous economic losses (Hui et al., 2007;Xianming et al., 2013;Xu et al., 2006;Yu, Huang, et al., 2014). Various countermeasures against A. adenophora invasion have been implemented, including chemical control and biological control based on its invasion mechanism; however, no single control approach is effective (Yang et al., 2017).
Preventing the invasion of IAS into new potentially suitable regions is thought to be the most effective way of controlling the damage and costs to both the ecosystem and economy (Fournier et al., 2019). SDMs play an important role in risk assessment and conservation (Jiménez-Valverde et al., 2011) as they can be used to investigate the relationships between species occurrence data and the background environmental conditions (Yue et al., 2019).
Predictions can then be made based on these relationships (Galletti et al., 2013;Yang et al., 2013;Zhang et al., 2018). The prediction of potentially suitable areas for species makes it possible for policymakers to enact measures to prevent IAS invasion. Numerous modeling methods are available for prediction, including the generalized linear model (He, Chen, et al., 2019), evolutionary algorithms (Gobeyn et al., 2019), random forest (Fern et al., 2019), Bayesian hierarchical logistic mixed model (Rocchini et al., 2019), and the maximum entropy (MaxEnt) model (Phillips et al., 2017). Although it is difficult to identify the most appropriate method (Elith et al., 2010), MaxEnt was applied in this study because of demonstrated ability to predict species distributions and superior performance compared with other presence-only SDMs (Abolmaali et al., 2018;Galletti et al., 2013;Qin et al., 2017;Tererai & Wood, 2014;Yi et al., 2016;Zhang et al., 2018).
This study aimed to address the following questions: (i) What are the potential spatial patterns of A. adenophora under current conditions and under different future climate change scenarios? (ii) Where and sensitive to climate change, and increasing human activities provide more opportunities for IAS invasion.

K E Y W O R D S
Ageratina adenophora, climate change, ecological niche modeling, invasive alien species, MaxEnt are the high-invasion-risk regions at present and in the future? We hope that the findings of this study contribute to preventing the further invasion of A. adenophora.

| Environmental variables
For climate data, 19 bioclimatic variables were obtained from the WorldClim dataset (http://www.world clim.org/), with a 1-km spatial resolution (Hijmans et al., 2005). The WorldClim dataset has been widely used in species distribution modeling (He, Su, et al., 2019;Jiao et al., 2019;Tan et al., 2019;Yue et al., 2019) (Gent et al., 2011). To indicate future climatic scenarios, we chose the data for 2050 and 2070 under four representative concentration pathways (RCPs): RCP2.6, RCP4.5, RCP6.0, and RCP8.5 (Ahmad et al., 2019). Soil data were downloaded from (http://soilg rids.org) at a resolution of 1 km, and 12 soil variables were selected to indicate the soil conditions (The PLOS One Staff, 2014). Terrain factors alter the redistribution of precipitation and solar radiation, resulting in mountain climate patterns. As previous research indicated that mountain ecosystems are more sensitive to climate change (Steinbauer et al., 2018), which is expected to trigger an upward expansion of plants in mountain regions (Grabherr et al., 1994;Walther et al., 2002). This finding has been proved that many native species have already shifted their distributions to a higher elevation (Chen et al., 2011;Lenoir et al., 2008). In addition, both habitat and elevation can restrict species ranges (Harris & Pimm, 2008;Sekercioglu et al., 2008) and have shown to be important in explaining the distribution of species (Luoto & Heikkinen, 2008;Virkkala et al., 2010). For the purpose of exploring the potential impacts of terrain factors, thus we added the topo covariate, including elevation, slope, and aspect (Manzoor et al., 2018). Terrain factors were derived from digital elevation model data, which were downloaded at (http://srtm.csi.cgiar.org/) and included elevation, slope, and aspect. We obtained land-cover data at 1-km resolution from the EarthEnv dataset (https://www.earth env.org/landc over), which integrates multiple global land-cover datasets (Tuanmu & Jetz, 2015).
For many applications in biodiversity and ecology, existing remote sensing-derived land-cover products are limited by inconsistency issues and their typically noncontinuous nature. The consensus product with the generalized scheme better captures land-cover heterogeneity and has improved utility for modeling species distributions. Two versions of the dataset are available: the full version and reduced version. The former dataset integrates GlobCover (2005-06; v2.2), the MODIS land-cover product (MCD12Q1; v051), GLC2000 (global product; v1.1), and DISCover (GLCC; v2); the latter only includes the first three datasets. In this study, we used the full version which includes 12 land-cover classes. The values of each land-cover class range from 0 to 100, representing the consensus prevalence in percentage.
To avoid model overfitting caused by multicollinearity between the selected variables (Dormann et al., 2013), Pearson's correlation analysis was performed and only those variables with correlation coefficient (r 2 ) < 0.75 were selected (Appendix S1, Table S1). For instance, if the absolute value of the cross-correlation coefficient between two variables exceeded 0.75, only the variable that captured more information was selected (Table 1). First, variables that have an R spearman less than 0.75 were retained, including bio2, bio15, bio18, and bio19. Actually, if a specific species is studied, among the highly correlated predictors we can retain the variable that has the highest correlation with species occurrence data (Manzoor et al., 2018).
Then, we considered the less collinear variables and selected the variable that captured more information. For example, bio14 (precipitation of driest month) and bio17 (precipitation of driest quarter) are highly correlated (R spearma =0.99, Table S1). Finally, bio17 was selected because of stronger explanation strength than bio14 according to Datta et al. (2019).

| Species occurrence data
Species occurrence data were downloaded from the Global Biodiversity Information Facility (https://www.gbif.org/, accessed 03 September 2018) and the Chinese Virtual Herbarium (http:// www.cvh.ac.cn/, accessed 03 September 2018). Furthermore, we collected 10 samples from Gyirong and Nyalam counties, which are adjacent to Nepal, during the fieldwork in 2016. A total of 5,474 occurrence points were initially recorded. Occurrence records are often biased toward geographically convenient or environmentally friendly (e.g., areas near cities or areas with high population density), resulting in sampling bias in geographic space. Thus, spatial thinning was performed to remove the spatial autocorrelation and sampling bias. Grid cells with dimensions of 10 × 10 km were created, and a single occurrence point was selected randomly from each cell with more than one occurrence point (Ahmad et al., 2019).
A total of 741 unbiased occurrence data points from regions in Asia

| Modeling approach and spatial analysis
We applied Maxent, version 3.3.3k (available at http://biodi versi tyinf ormat ics.amnh.org/open_sourc e/maxen t/; Phillips et al., 2006) to predict the potential suitable area of A. adenophora. As one of the most effective presence-only algorithms available, Maxent has been shown to perform better than other models, and it is quite robust when there are a small number of occurrence points (Elith et al., 2006;Hu et al., 2015;Jarnevich et al., 2010;Wisz et al., 2008;. Seventy percent of the occurrence points were selected for model training, while the other 30% were used for model validation. The model output represented the probability of presence from 0 to 1 (Phillips & Dudík, 2008). The area under the curve (AUC) of the receiver operating characteristic (ROC) curve was used to evaluate the model performance. The AUC value ranges from 0 to 1, an AUC value between 0.5 and 0.7 indicates that the model performance is not acceptable, AUC in the range of 0.7-0.9 indicates good performance, and AUC > 0.9 indicates the highest predictive ability (Abdelaal et al., 2019;Phillips et al., 2006). Furthermore, we also calculated the true skill statistics (TSS) to estimate the model performance (Allouche et al., 2006;Fielding & Bell, 1997;Swets, 1988). As a threshold-dependent metric of model evaluation, TSS ranges from −1 to +1 and values above 0.75 indicate excellent model performance (Allouche et al., 2006).
The most commonly used framework combines occurrence records from both the native and introduced regions by using distribution data from the native range, this strategy makes use of those occurrence records that are likely to be in equilibrium with the regional environment while also including records from introduced regions which may provide additional information about expansion into novel ranges (Marcelino & Verbruggen, 2015;. Four arbitrary categories of invasion risk for A. adenophora were defined as no risk (NR, <0.2), low risk (LR, 0.2-0.4), moderate risk (MR, 0.4-0.6), and high risk (HR, >0.6) based on predicted habitat suitability Zhang et al., 2018Zhang et al., , 2019. In this study, we defined a region as an under-risk (UR) region when its risk category was LR, MR, or HR. Furthermore, Ageratina adenophora is native to Mexico; therefore, occurrence of this species in Mexico is not due to invasion. Thus, we masked out Mexico when calculating the UR regions. Based on the predicted results for the current climate conditions and eight RCPs, the risks of invasion by A. adenophora in different areas were calculated using ArcGIS 10.4.1 based on the four arbitrary categories defined above. To explore the variation in the distribution of A. adenophora with altitude under climate change scenarios, we calculated the areas and area ratio of LR, MR, HR, and UR in different elevation ranges under climate change scenarios and applied the "ecospat" package in R to visualize these changes (Di Cola et al., 2017).

| Model performance and main variables
The AUC value for A. adenophora obtained using the MaxEnt model         increases were found in regions with elevations between 3,000 and 3,500 m, increased by an average of 132.11%. According to the above results, we found that regions with elevations between 3,000 and 3,500 experienced the greatest growth of all risk ranks.

| Dynamics in potential suitable area of A. Adenophora under climate change scenarios
To analyze the potential suitable area shifts of A. Adenophora, a further analysis about the potential suitable areas of A. Adenophora along with the elevation is depicted in Figure 8.  Density change scenarios; this number is 2.35% in areas at elevations between 1,000 and 1,500 m and 2.35% in areas at elevations between 1,000 and 1,500 m. When the altitude is higher than 1,500 m, percentages of UR areas increased and the greatest average increase was found in elevation ranges between 2,500 and 3,000 m. The same trend was also observed in MR and HR areas (Appendix S2, Figure S1 and Table S2). The percentages of MR and HR areas at elevations between 500 and 1,500 m clearly decreased. The greatest decrease was found in elevation ranges between 500 and 1,000 m, with an average decrease of 10.82% and 5.97%, respectively. Percentages of MR areas increased when areas with elevations above 1,500 m and the greatest increase were observed in elevations ranges between 2,000 and 2,500 m. Percentages of HR areas increased when areas with elevations between 1,500 and 4,000 m except areas with elevations between 1,500 and 4,000 m of RCP8.5 in 2070. Percentages of LR areas decreased when areas at elevations below 1,500 m and the greatest decrease were found in elevations ranges between 500 and 1,000 m (Appendix S2, Figure S3). When the altitude is higher than 1,500 m but lower than 4,000 m, percentage of LR increased compared with current conditions. Furthermore, we found similar potential suitable areas shift of A. adenophora under the future climate change scenarios in the regional scale. Mountains of Southwest China BHR (Figures 9 and   10), which has suffered severe damage due to the invasion of A. adenophora, with areas at elevations of 2,500-3,000 m accounting for the largest proportion of UR areas (

| D ISCUSS I ON
IAS has caused enormous economic losses and threatens biodiversity globally. The continental accumulation of IAS is predicted to increase by 36% from 2005 to 2050 (Seebens et al., 2020). The most effective way to prevent damages caused by IAS is to predict their potential suitable area and take measures to limit their spread to new areas (Fournier et al., 2019). A. adenophora has proven to be a very aggressive invasive species in some parts of the world, including China, Australia, and South Africa. These regions have enacted costly measures to control the spread of A. adenophora. Therefore, it is of great significance to predict the potential suitable area patterns of A. adenophora under current climate conditions and future climate change scenarios.
SDMs have been widely applied to predict the potential suitable areas of IAS based on niche conservatism, which assumes that an IAS will retain a similar niche in the native and introduced regions (Ahmad et al., 2019;Graham, 2005). Although it is still controversial whether species niches are conserved across space and time (Atwater et al., 2018), recent research supports the niche conservatism hypothesis overall (Liu et al., 2020). The MaxEnt model has been widely applied in simulating species distribution Merow et al., 2013). In this study, we built nine MaxEnt models according to the species occurrence data and climate data under cur-

| Effect of temperature change on the distribution of A. adenophora
Previous studies have shown that A. adenophora is invasive in tropical and subtropical regions, including Asia (China, India, and Nepal), Oceania (eastern Australia and New Zealand), Africa, and North America (Cronk & Fuller, 1995;Del Guacchio, 2013;Heystek et al., 2011;Kluge, 1991;Muniappan et al., 2009;Parsons et al., 2001;Tererai & Wood, 2014;Wang & Wang, 2006), and our results concur with these findings. Furthermore, we found that over 70% of the UR areas are distributed in the 36 BHRs, which are distributed in tropical and subtropical regions. Previous studies have shown that the expansion of IAS might become apparent later in invasion events and consequently have extensive negative effects on native species and the overall stability of native ecosystems (Adams & Setterfield, 2015;Mainali et al., 2015;Pyšek et al., 2012;Roger et al., 2015;Vicente et al., 2013). From this point of view, the invasion of A. adenophora may have serious consequences in these regions.
According to the growth environment of this weed and previous studies, the temperature is the major factor controlling the distribution of A. adenophora. A study by Wang, Lin, et al. (2017) found that the temperature during winter is the most influential factor affecting the distribution of A. adenophora in China. The research results of (Thapa et al., 2018) showed that the Minimum Temperature of Coldest Month is the most significant variable in western Himalaya. Among environmental factors, temperature, particularly the low temperature, is the main factor governing the distribution of A. adenophora Wang, Lin, et al., 2017).
The above-mentioned studies support our finding that mean temperature of coldest quarter (Bio11)  Active restoration interventions are generally restricted by funding and thus self-repair ability of ecosystem is expected to work.
Nonetheless, is spontaneous succession a viable strategy? (Holmes et al., 2020) pointed out that the ecosystems can accomplish selfrepair under the conditions which key biotic and/or abiotic thresholds have not yet been crossed. Specifically, the identity of the invader, the ecosystem type, and the efficacy of alien control would influence this process. For example, some species can alter the soil conditions to favor its growth and release chemical drift to constrain native species (Gaertner et al., 2012;Krupek et al., 2016).  (Yang, 2008;Zhong et al., 2007) and can alter soil microbial communities in its favor Yu et al., 2005).
Furthermore, different restoration solutions are required for different ecosystems. For instance, lowland fynbos ecosystems are said to be less resilient to invasion and have a lower capacity for self-repair compared with mountain fynbos ecosystems (Holmes et al., 2020).
This means that active restoration is necessary for these areas of low self-repair capacity. Anyway, large capital costs are required for restoration, thus preventing invasions early is vastly preferable.

| Whether
A. adenophora will shift toward higher elevation under future climate change scenarios?
Under global warming, some species will migrate to higher latitudes or higher elevations to adapt to climate change (Bertrand et al., 2011;Hackett et al., 2008;Root et al., 2003), especially in mountain ecosystems (Felde et al., 2012). Under current climate conditions, the distribution of A. adenophora with respect to elevation is similar in native and introduced regions. A. adenophora is distributed in areas with elevations ranging from 520 to 3,200 m in its native range (Mexico) (Sang et al., 2010), while it is found at elevations between 330 and 2,500 m in China (Wang & Wang, 2006)  percentages at different elevation ranges, the potential suitable area of A. adenophora would expand in elevation ranges between 3,000 and 3,500 m. In combination with a decreasing trend globally, a likely explanation is that A. adenophora will shift upslope under future climate conditions and thus face consistent reductions in the area that this species can occupy (Liang et al., 2018). Though previous studies have indicated that the species toward higher elevations or latitudes is predicted to increase with climate change, most of the evidences were observed from the occurrence records collected from the fields (Dainese et al., 2017;Kelly & Goulden, 2008;Steinbauer et al., 2018;Vanderwal et al., 2013). We predicted the expansion of A. adenophora at higher elevation ranges, which could not figure out the drivers of this kind of expansion (from lower area or not).
Biological invasions are considered to be the 5th important impact of human activities on the earth's environment (Brondizio et al., 2019). Montane ecosystems, which have high biodiversity and are sensitive to climate change, are of particular concern under climate warming (Dullinger et al., 2012). Among terrestrial ecosystems, mountain ecosystems and particularly high mountains are often considered to be at low risk of invasion (Pauchard et al., 2009). However, the invasion process is driven by a combination of climate change and human activities (Alexander et al., 2016). Increasing anthropogenic activities offer more opportunities for the invasion of non-native species, and road networks are regarded as the major pathway for IAS invasion. There will be at least 25 million kilometers of new roads anticipated by 2050, with developing countries accounting for 90% of this increase (Laurance et al., 2014). This will provide opportunities for the establishment of non-native species and conduits for their dispersal (Becker et al., 2005); roads and trails are recognized as major pathways for invasion into mountains (Fuentes et al., 2010;Lembrechts et al., 2014;Pauchard & Alaback, 2004). Hence, a detailed assessment of the effects of road infrastructure on biodiversity is needed given the rapid expansion of road networks.

| Uncertainty
The limitations of this study can be summarized as follows. Since MaxEnt is an ecological niche model, only the abiotic factors were taken into consideration (Ahmad et al., 2019;Xu et al., 2019). As indicated by the "BAM" (abiotic factors, biotic factors, and movement) diagram (Pauchard & Alaback, 2004), the distribution of a species is governed not only by abiotic factors but also by biotic factors including interactions between species and dispersal ability. It should also be noted that we only used MaxEnt model in this research instead of using an ensemble model, some research found that ensembles outperform individual models (Crossman & Bass, 2008;Marmion et al., 2009). In this study, the land-cover conditions along with climate variables were used as input to the model; however, we assumed that the land-cover conditions would remain unchanged in the future.
Climate factors were considered to be the principal factors in other global-or country-scale studies of species distribution. To better understand the influence of climate change on species distribution, the intraspecific interactions and changes in land cover should be taken into consideration. Furthermore, the current climate conditions in this study are not "current" for the current climate data derived from interpolations of observed data (representative of 1960-2000).
During the past two decades, the world climate has changed greatly, which may affect the accuracy of the model . The newly released CMIP6 applied a new set of emissions scenarios, shared socioeconomic pathways (SSPs; O'Neill et al., 2017), is said to make future scenarios more reasonable and thus more reliable than before (Di Luca et al., 2020;Nie et al., 2020;Su et al., 2021). Finally, although we have determined the regions of native occurrence from all records, the intentional introduction of A. adenophora was not taken into consideration. This may explain why the occurrence of A. adenophora is always near urban/built-up regions.

| CON CLUS IONS
Detecting the potential suitable regions for species invasion is of great significance for preventing IAS invasion. Based on the MaxEnt model, the potential invasion ranges of A. adenophora under current and future climate conditions were evaluated. Our results show that the potential invasion range of A. adenophora is mainly distributed in subtropical and warmer temperate regions, including southwestern America, Chile, the Himalayas, southwestern China, and southeastern Australia. Among environmental factors, the mean temperature of coldest quarter contributes the most to the model, and the optimal temperature range for this species is 8°C-16°C. Although the invasion range of A. adenophora will shrink globally under all RCPs, the invasion risk will increase in six biodiversity hotspot regions (BHRs), such as Mountains of Southwest China, with a clear expansion trend at higher elevations under future climate scenarios. The findings provide reference information for developing appropriate management strategies to prevent the establishment and further spread of A. adenophora across the globe, especially in BHRs. Research findings in our study call for special concern on biological invasions in BHRs, especially in mountain regions.

ACK N OWLED G M ENTS
We would like to express our thanks to the anonymous reviewers for their helpful comments on our paper. We would also like to express our special thanks to Mr. Investigation and risk assessment of exotic invasive species in Xizang (II): ZD20170021.

CO N FLI C T O F I NTE R E S T
The authors declare that they have no conflict of interest.  DZj9T 8dRdT uNJvr j2dt0 G9ZZv sdhsN ueE07Wt8).