Hydrolytic enzymes and their directly and indirectly effects on gluten and dough properties: An extensive review

Abstract Poor water solubility, emulsifying, and foaming properties of gluten protein have limited its applications. Gluten is structured by covalent (disulfide bonds) and noncovalent bonds (hydrogen bonds, ionic bonds, hydrophobic bonds) which prone to alteration by various treatments. Enzyme modification has the ability to alter certain properties of gluten and compensate the deficiencies in gluten network. By hydrolyzing mechanisms and softening effects, hydrolytic enzymes affect gluten directly and indirectly and improve dough quality. The present review investigates the effects of some hydrolytic enzymes (protease and peptidase, alcalase, xylanase, pentosanase, and cellulase) on the rheological, functional, conformational, and nutritional features of gluten and dough. Overall, protease, peptidase, and alcalase directly affect peptide bonds in gluten. In contrast, arabinoxylan, pentosan, and cellulose are affected, respectively, by xylanase, pentosanase, and cellulase which indirectly affect gluten proteins. The changes in gluten structure by enzyme treatment allow gluten for being used in variety of purposes in the food and nonfood industry.

| 3989 POURMOHAMMADI AnD ABEDI contain considerable amounts of glutamine/glutamic acid, proline, and phenylalanine and are completely deficient in sulfur amino acids (Majzoobi, et al., 2012). Therefore, the low gluten solubility is due to the small number of ionizable amino acids and high amounts of glutamine, proline, and glycine in gluten construction.
Despite the myriad interesting functionalities of wheat glutens, this protein has poor water solubility, emulsifying, and foaming properties which have limited its applications. There are various methods for improving the functionality of glutenin and gliadin by modifying their structure. As a biotechnological treatment, enzyme modification is one of the methods which extends gluten applications.
There are numerous limitations in using flours with strong gluten networks; therefore, the hydrolyzing effects of enzymes allow to use these flours in various purposes. Improvements in bread properties obtained by the addition of hydrolytic enzymes have been associated with their impact on the physical properties of the dough during processing. One of the major functions of hydrolytic enzyme addition is to soften dough to improve machining properties and thus enhance bread quality (Figure 1; Harada et al., 2000;Pourmohammadi & Abedi, 2021;Yong et al., 2006).
The present review investigates the modes of action of some hydrolyzing enzymes and their effects on the functional, rheological, conformational, and nutritional characteristics of gluten and dough.
F I G U R E 1 Application of hydrolytic enzymes on dough structure (a) (b) (c)
In addition, protein hydrolysates are extensively utilized as functional ingredients in food and chemical industries. Some studies, on the other hand, showed that proteolytic enzymes had undesirable impacts on the volume of bread because of the disruption in gluten matrix, particularly glutenin subunits (Kolpakova et al., 2014).
Moreover, the combination of hydrolyzing enzymes with different treatments and additives has considerable effects on gluten properties (Table 1, 2). Heat treatment +papain Reduces the free SH in wheat gluten proteins

| Sources of proteases and peptidases for gluten hydrolyzation
Makes the structure of wheat gluten more compact (Wang et al., 2009) Heat treatment +alcalase Increases the rate of hydrolysis Improves gluten susceptibility to alcalase owing to the rearrangements of the interand intramolecular binding (Mohamed Bashir Elmalimadi, 2018;Saha et al., 2013) Heat treatment +alcalase Enhances the emulsifying properties of gluten Exposes the hydrophobic protein interior, improving the adsorption at the interface, forming a cohesive interfacial film with the hydrophobic residues (Mohamed Bashir Elmalimadi, 2018;Phillips & Beuchat, 1981) s Heat treatment +alcalase Improves the foaming characteristics Increases polypeptide chains arising from partial proteolysis, incorporating more air (Mohamed Bashir Elmalimadi, 2018;Kong et al., 2007a;Wouters et al., 2016) Heat treatment +alcalase Increases solubility and waterholding capacity Augments cleavable peptide bonds and increases the number of exposed ionizable amino and carboxyl groups (Mohamed Bashir Elmalimadi, 2018;Hardt et al., 2013) Heat treatment +alcalase Reduces the binding affinity to fat Hydrolytic degradation of the protein structure (Mohamed Bashir Elmalimadi, 2018) Heat treatment +alcalase Increases the DPPH radical scavenging activity (antioxidant ability) Opens and exposes active amino acid residues, which could react with oxidants or reactive oxygen (Mohamed Bashir Elmalimadi, 2018;Koo et al., 2014) Agitation +alcalase Ameliorates the efficiency of gluten hydrolysis Reduces the particle size and increases the surface area (Mohamed Bashir Elmalimadi, 2018) Temperature (50 0 C) + pH 9 + gluten +alcalase

Improves gluten solubility
Enhances foaming stability of gluten Reduces the molecular weight and hydrophobicity of wheat protein and increases the content of polar and ionizing groups (Jakovetić et al., 2015) Pancreatin hydrolysis/ Extrusion Enhances the enzymatic hydrolysis efficiency of wheat gluten The conformational changes and structural rearrangements of wheat gluten treated with extrusion might modify the catalytic sites of proteases (Cui, Gong, et al., 2013) A. flavus var. oryzae), bacteria (Flavobacterium meningosepticum,  (Merz et al., 2015). Wheat gluten (25 g/L) treated with flavourzyme led to 9.5 mg/kg residual gliadin concentration in the dried hydrolysate, which, through filtration, could be further decreased to around 2 mg/kg (Eugster et al., 2015;Merz, Kettner, et al., 2016).
Increase in the degree of hydrolysis (DH) was reported to augment the solubility of hydrolysates obtained from A. oryzae with a fungal protease; however, foaming features are seemingly impaired beyond a certain DH (14%) (Drago & González, 2000). Another study reported that to obtain desirable emulsifying and foaming properties, the DH should be even lower, 5% to be exact (Brzozowski, 2016;Kong et al., 2007b).
Prolyl endopeptidase from A. niger, which called Aspergillus niger prolyl endopeptidase (AN-PEP) (EC 3.4.21.26), has postproline cleaving activity. It is resistant to digestion by pepsin and active in a pH range of 2-8 with optimum activity at pH 4-5; moreover, AN-PEP is highly able to effectively degrade CD-active peptides along with intact α-gliadins, γ-gliadins, HMW-GS, and LMW-GS (König Starch +flavourzyme + protamex starch granules impede gluten aggregation, which facilitates the hydrolysis Hinders the gluten aggregation (Hardt et al., 2015) Enzymatically hydrolyzed gluten +sucrose Improves the stability and foaming capacity of hydrolyzed gluten Increase the affinity of hydrolyzed gluten and adsorption at the water-air interface  Ethanol +trypsin or pepsin Increases the foaming capacity and reduces the foam stability of gluten Alters the air-water interfacial behavior of gluten  Cysteine +alcalase Enhances gluten hydrolysis Alters gluten viscoelastic behavior (varying from more solidlike to more fluidlike) and increases its solubility (Zhang et al., 2012) TA B L E 2 Effects of different additives in combination with hydrolyzing enzymes on gluten properties (1) TA B L E 3 Different sources of proteases and peptidases for gluten hydrolyzation Type of enzyme Object Approach Ref.

Fungal peptidases (Fusarium graminearum)
Aspergillus usamii protease Wheat gluten Increasing the protein hydrolysates solubility resulting from its secondary structure destruction (Deng et al., 2016) Aspergillus usamii protease Wheat gluten The cleavage of peptides by enzyme and unfolding the globular structure of gluten were able to promote the crosslinking between peptides-lipid, and contribute to anchoring the peptide molecules at the oil-water interface, improving the emulsifying properties and decreasing the interfacial tension (Deng et al., 2017) Aspergillus usamii protease Wheat gluten Increase the water-holding capacity of wheat gluten/ increase in β-sheet ratio (Saberi et al., 2008) (Continues) et al., 2017; Stepniak et al., 2006;Kara et al., 2005;. It is also expected that protease therapy with AN enhances the symptoms of nonceliac gluten sensitivity because indigestible gluten-related proteins have been observed to trigger nonceliac gluten sensitivity (Ido et al., 2018 Wheat gluten Gluten degradation with postproline cleaving patterns (Mika et al., 2015) Endopeptidases from Pentadomidae and Lygaidae, Nysius huttoni HMW-GS The cleavage of peptides by enzyme, unfolding the globular structure of gluten, promoting the cross-linking between peptides-lipid, contributing to anchoring the peptide molecules at the oil-water interface, lead to improve emulsifying properties and the interfacial tension decrease (Koksel, 2001) Endopeptidases from Eurygaster Aelia, E. Maura, E. integriceps

Glutenin
Increase the oil holding capacity/ enzymatic hydrolysis cause hydrophobic regions, to be more exposed to the aqueous phase (Yakovenko et al., 1973) Bug proteolytic enzymes Glutenin Reduction in the certain bonds intensity and creation of two new bands in the electrophoretic patterns according to SDS-PAGE (Sivri et al., 1998;Yakovenko et al., 1973) TA B L E 3 (Continued) and sourdough (Walter et al., 2015), and beer .
Aspergillopepsin (EC 3.4.23.18) from A. niger further showed glutendegrading activity; compared with AN-PEP, however, it was not nearly as substrate-specific and efficient, hence the fact that it might only be utilized complementary to Endoprotease B, isoform 2 (EP-B2), or AN-PEP, for instance (Ehren et al., 2009).
F. graminearum proteases are not only able to degrade gluten proteins in the grain itself, but also capable of weakening gluten during dough preparation and resting (Koga et al., 2019). These proteases were observed to be essentially trypsin-like serine proteases cutting the proteins at the lysine or arginine amino acid (Pekkarinen et al., 2007). The primary destruction of HMW-GS compared to LMW-GS is possibly explained by their comparatively higher amount of lysine or arginine. Other studies showed reduced glutenin amount in comparison with increased gliadin following F. graminearum infection in wheat grains (Eggert et al., 2011).
A. usamii protease affected gluten through increasing the solubility of protein hydrolysates resulting from its secondary structure destruction and the enzymatic release of smaller polypeptide units from the protein (Deng et al., 2016). The cleavage of peptides by enzyme and unfolding of wheat gluten's globular structure were able to promote the interaction between peptides and lipid and contribute to anchoring the peptide molecules at the oil-water interface. This increased the emulsifying activity and decreased the interfacial tension (Deng et al., 2017). The ratio of turns decreased, and the globular structure of gluten was unfolded due to the enzymatic cleavage of peptide chains; therefore, longer β-sheet chains were generated and the β-sheet ratio increased (Barth & Zscherp, 2002). A. usamii protease slightly increased the water-holding capacity of wheat gluten from 1.47 to 1.75 g/g; however, after hydrolysis, the holding capacity of oil was significantly increased from 0.92 to 2.91 g/g. This is possibly associated with the enzymatic hydrolysis exposing more hydrophobic regions (originally buried within the wheat gluten) to the aqueous phase (Saberi et al., 2008).

Bacterial peptidases
Lactic acid bacteria have a highly convoluted peptidase system (Kunji et al., 1996;M'hir et al., 2012); however, it is not a unique strain possibly possessing the whole pattern of peptidases required to hydrolyze all the potential peptides in which the protein is involved. B.
Hydrolyzed protein obtained from wheat gluten treated with alcalase possess the maximum degree of hydrolysis (15.8%) values and is more effective in gluten hydrolysis compared with pepsin, pancreatin, neutrase, and protamex (Kong et al., 2007a (Gass et al., 2007), and Lactobacillus helveticus (Chen et al., 2003), resulted in similar properties (gluten detoxification). (e) they can be integrated into production processes in a relatively facile manner, and (f) they are well accepted by consumers. As far as drawbacks are concerned, the gluten-degrading activity of cereal extracts was much lower than purified enzymes (Walter et al., 2014) and their activity was inhibited by ethanol (≥ 2%) .

| Plant peptidases
Taken together, the upsides clearly outweigh the downsides; applying gluten-degrading cereal peptidases is a promising method for generating high-quality gluten-free products derived from glutencontaining cereals, including gluten-free and barley-based beer .

| Effect on functional properties
By using hydrolyzing enzymes, the emulsifying capacity of gluten augments which is ascribe to changes in the secondary structure of

Effect
Ref.

Protease Gluten
Degrading proteins, water binding capacity reduction, excess released water, reduction in dough viscosity, and production of softer dough Wheat dough Diminish the storage (G΄) and complex (G*) modulus, weakening effect, decrease in resistance to extension (Caballero et al., 2007;Harada et al., 2000;Indrani et al., 2003) Bacterial peptidase/ fungal peptidase/ prolyl endopeptidases (PEPs)  (Lu et al., 2015) wheat gluten (Sun et al., 2019) depending on the degree of hydrolysis and protease activity (Deng et al., 2016). Increasing the emulsifying capacity of gluten in hydrolyzed samples can be explained by the unfolding of wheat gluten's globular structure and peptide bond's disruption by enzymatic modifications. Disruption of peptide bonds could facilitate the interaction among peptides and lipids and increase the availability of peptide residues at the oil-water interface leading to reduced interfacial tension and increased emulsifying activity. Nonetheless, moving further in the proteolytic activity, a reduction occurs in the emulsifying capacity of the hydrolysates due to extensive gluten degradation . The foaming capacity of hydrolyzed gluten was significantly improved by elevating the surface activity and reducing the surface strain at the water-air interface. This is possibly attributed to the large amount of polypeptide chains having broad molecular weight distributions generated from partial proteolysis, hence incorporating more air in wheat gluten (Deng et al., 2016) and enhancing the flexibility induced by the reactions of SS/SH interchange (Wouters et al., 2016). On the other hand, foam stability was reduced in more extensive hydrolysis due to the increased polypeptide chains unable to make foam air cells stable Wouters et al., 2016;. Gluten treated with alcalase was further reported to increase solubility, emulsifying capacity, foaming stability, and foaming capacity owing to produce lower molecular size hydrolysates (Elmalimadi et al., 2017;He et al., 2019;Kong et al., 2007b). Deng et al., (2016) revealed that enzymatic hydrolysis is capable of breaking some SS bonds to unfold the globular structure of gluten and new SS bonds are generated between both newly generated SH and original free SH groups to stabilize the structure of smaller peptides.

| Effect on rheological properties
According to SDS-PAGE profile, the molecular weight of all the wheat gluten hydrolysates is drastically decreased (Figure 2) in the hydrolyzed gluten proteins . Researchers have revealed that the hydrophobicity of hydrolyzed protein is able to augmented or reduced based on enzyme specificity, hydrolysis situations, protein characteristics, and degree of hydrolysis . At low enzyme concentrations, the hydrophobicity of hydrolyzed gluten, augmented, which could be due to the hydrophobic amino acid exposure through protein unfolding . In excessive hydrolyzing treatment, hydrophobicity would decrease following two possible reasons: (1) degradation of some exposed hydrophobic regions; (2) burying the hydrophobic amino groups due to hydrolysis reactions (Zhang et al., 2014). As a regard to FTIR results, adding alcalase to wheat gluten reduces α-helix and β-turn conformation (Cui, Gong, et al., 2013).
The reduction is due to the intermolecular disulfide bonds breakage and the elevation of β-sheet and random coil caused by the alcalase hydrolyzation of the β-turns into random coils (Cui, Gong, et al., 2013).
Also, Wang et al. (2016) proposed that an acceptable amount of alcalase-based hydrolysis unfolded the rigid structure of wheat gluten and increased the β-sheet content.
Based on researches, alcalase-based partial hydrolysis broke SS bonds, thereby unfolding the protein conformation of wheat gluten and increase the SH content. However, excess hydrolysis exposed many hydrophobic amino acids and formed aggregates belonging to wheat gluten hydrolysate. Besides, free SH groups might have participation in forming such aggregates . The antioxidant activity of gluten hydrolyzed by alcalase was also studied, where the antioxidant activity of wheat hydrolysates has positive correlation with the content of hydrophobic amino acids. In other words, by excess hydrolysis, due to the increase in hydrophobic amino acids, the antioxidant activity of gluten would increase .
The impact of peptidase hydrolysis on rheological properties is reported in different researches (Ahmed & Ikram, 2015;Koga et al., 2019). Peptidase hydrolysis reduced storage modulus (G´) (Ahmed & Ikram, 2015) and gluten consistency (Koga et al., 2019) due to gluten degradation and digesting effect of protease. In the gluten samples treated with protease, the glutenin polymer size is notably decreased which leads to reduction in maximum resistance to extension (R max ). Rheological results reveal that glutenin polymer's size reduction by proteolytic hydrolysis in treated flours destroyed gluten structure (Koga et al., 2019).
The interactions among flour components (proteins, starch, fibers, etc) play key roles on the rheological properties of dough.
According to researches, proteases diminish the storage (G΄) and complex (G˝) modulus. The weakening effect of proteases on wheat dough relates to the decrease in resistance to extension observed by Indrani et al., (2003). Proteinase activity affects specially to glutenins, which would alter the elasticity of the gluten complex (Caballero et al., 2007). Dough prepared with high levels of protease felt sticky and weak, which probably accounts for its poor performance. This weakness can be attributed to the hydrolysis of gluten proteins which are known to be the major determinant of dough strength (Harada et al., 2000). The hydrolyzing mechanism of protease enzymes results in degrading proteins as enzyme substrate.
Hydrolyzed proteins lead to water binding capacity reduction, consequently excess released water, which cause significant reduction in dough viscosity and production of softer dough with better machining properties (Harada et al., 2000). However, it has also been recognized that over enzyme addition can cause overly soft or sticky dough, resulting in machining problems at the sheeter and rounder that lead to a deterioration in bread quality (Harada et al., 2000).
Protease and peptidase can be used in bakery industry via their hydrolyzing mechanism (Table 4).
Furthermore, SEM analysis (Figure 4a) showed the damaged gluten structure, resulting in increased tan δ. tan δ is an indicator of protein quality (the higher the tan δ, the weaker the gluten network structure) (Kong et al., 2007).

| Effect on thermal properties
Hydrolysis by proteases influenced wheat gluten thermal stability, determined by DSC. The glass transition temperature (Tg) is normally related to the protein thermal stability (more Tg values associated with enhanced wheat gluten thermal stability). Several studies were reported that alcalase significantly increases Tg values due to high quantities of exposed hydrophobic groups . Furthermore, Tg found to be lower, in excessive hydrolysis, due to splitting hydrophobic groups in to ionized groups. The change in enthalpy (ΔH) shows the extent of arrangements in the protein structure and straightly have relationship with its denaturation. Partially hydrolyzed gluten with alcalase significantly decreases ΔH parameter due to the alteration of the tertiary structure of gluten and less heat energy requirement (Wang, Qin, et al., 2017).

| Effect on sensory characterization
Peptides with more hydrophobic amino acids are more likely to have lower bitter taste thresholds. When wheat gluten was hydrolyzed for 300 min by Proteax (a proteolytic enzyme obtained by Aspergillus oryzae), the hydrolyzed gluten protein showed minimum bitterness but maximum content of small peptides varying from 180 to 500 Da (He et al., 2019;Riu &Riu, 2016).

| Effect on celiac disease
Uncontrolled immune response to wheat gluten causes a chronic enteropathy called celiac disease (CD) which refers to the pathology of the intestine. There are more than 60 immunogenic peptides in gluten derived from Triticum species. 33-mer peptide with 13 proline residues and 10 glutamine residues is the most important immunogenic peptides, which is resistant to enzymatic proteolysis.
Gliadin fractions and other wheat proteins can act as allergens; thus, celiac disease patients are not capable of tolerating these proteins (Heredia-Sandoval et al., 2016;Bethune et al., 2006). Celiac disease patients carry HLA-DQ2 and/or -DQ8 serotype which has the affinity to connect to antigens like gliadins and increase T-cell-mediated autoimmune reaction (Figure 3).
On the contrary, many of the recent investigations which employed enzyme-linked immunosorbent assay (ELISA), mass spectrometry, and Western blot analysis reported that PEP did not thoroughly destroy the whole gluten proteins (Allred et al., 2017;Colgrave et al., 2017;Fiedler et al., 2018;Panda et al., 2015), which indicates that beers treated with PEP are not safe for CD patients.

F I G U R E 3
Adaptive immune response to nonmodified baked goods and nonactivation of T cells to modified baked goods by microbial proteases (Heredia-Sandoval et al., 2016)

| Xylanase
Xylanases (EC 3.2.1.8) are able to break glycosidic linkages in arabinoxylans, producing smaller fragments. Xylanase from various sources has different mechanisms: 1) Xylanase obtained from A. niger degrades water-extractable arabinoxylans exist in flour and reduces the molecular mass and dough viscosity of water-extractable arabinoxylans; therefore, enhances the F I G U R E 4 Scanning electron microscopy (SEM) of wheat gluten samples (A) WG (a), WG treated with desired quantity of Alcalase (0.04%, HWG-4; b) and (0.10%, w/w, HWG-10; c) . SEM of gluten (B) without enzymes (a), xylanase (b), and cellulase (c) . SEM images of dough treated with pentosanase (C), dough without pentosanase (a), and dough treated with pentosanase (b) (Steffolani et al., 2010) (Sun et al., 2019) F I G U R E 5 Xylanase reaction with gluten (Steffolani et al., 2010) gluten agglomeration behavior and the larger gluten aggregates formation; 2) Xylanase generated from B. subtilis solubilizes water-unextractable arabinoxylans augments the dough viscosity and negatively impacts gluten agglomeration (Romanowska et al., 2006). By removing arabinoxylans from gluten, xylanase alters the water distribution between gluten proteins and arabinoxylans, hence indirectly affect bread quality. Improving bread quality in the presence of xylanase also might be due to pentosan destruction and viscosity reduction effect of this enzyme. Viscosity reduction causes polymer chains to get next to each other easier, and the gluten aggregation would occur (Amiri et al., 2016). According to the competition between gluten and pentosan for water absorption, the degradation of pentosans by xylanase positively affects the gluten water binding characteristics ( Figure 5; Amiri et al., 2016). On the contrary, some researchers found no evidence of xylanase removing arabinoxylans from gluten (Steffolani et al., 2010). These researchers believe that the evidence do not demonstrate the cleavage of covalent bonds between arabinoxylans and gluten by xylanase (Amiri, et al., 2016). Steffolani et al., (2010) showed that endo-xylanases increased SDS-unextractable proteins, which can be explained by the importance of arabinoxylans in changing the extractability of the glutenin polymers ( Figure 4b and Figure 5). Arabinoxylan breakdown with xylanase resulted in a less viscous dough, thereby augmenting the protein fragments mobility and facilitates their hydrophobic connections. These interactions would cause a more rapid protein aggregation which is due to the removing of steric interruption of arabinoxylans. Xylanases also redistributed water from arabinoxylans to the gluten and starch phase, make water more available to plasticize protein, thereby helping the gluten development. This made the dough and bread crumb softer and positively influenced bread making (Altınel & Ünal, 2017;Harada et al., 2000;Nevsky et al., 2018). In certain studies, the dough hardness was clearly reduced by adding high xylanase dosage to flour. The reduced dough hardening indicated the impact of xylanase on the interactions between glutenin and water soluble pentosans which leads to decrease disulfide cross-linking and increase in SH content (Amiri, et al., 2016). Based on rheological results, xylanase tended to decrease storage modulus (G´) and augment tan δ which both inhibits less elastic behavior of dough in comparison with the control gluten samples. This phenomenon might be attributed to the degradation of pentosans and changing the gluten structure in samples treated with xylanase (Amiri et al., 2016;Steffolani et al., 2010). Moreover, the softening effect of xylanase is attributed to the release of water that occurs when arabinoxylans are hydrolyzed and the water binding capacity is reduced. The released water can reduce dough viscosity resulting in softer dough with better machining properties (Harada et al., 2000).

| Pentosanase
Pentosanase is responsible for the conversion of waterunextractable arabinoxylans to water-extractable forms. Sun et al., (2019) revealed that water-extractable arabinoxylans positively affect bread volume and textural properties, while water-unextractable arabinoxylans cause undesirable product quality due to the competition for water and hinders gluten formation during the development of dough (Figures 2D and 4C).
This conversion affects gluten network by five possible reactions: 1) Water-unextractable arabinoxylans have high waterholding capacity, which might cause lower water availability for gluten development, due to competition for water. Therefore, this conversion is known to improve gluten formation in baked products ; 2) The pentosan-protein network will be weakened during the conversion of water-unextractable arabinoxylans to their water-extractable form. The cleavage of pentosan-protein bonds would release water, which is necessary for gluten development Verjans et al., 2010); 3) Pentosanase would produce pentosans with smaller size which cause a redistribution of free water and reduce the steric hindrance of insoluble pentosans, thereby elevating the interaction between proteins (Steffolani et al., 2010); 4) Interaction among glutenin and water soluble pentosans lead to increase in SH content and dough softening according to SEM analysis ( Figure 4C; Steffolani et al., 2010); 5) the enzyme enhanced gluten coagulation through reducing the steric impediment of the pentosans related to gluten and counteracting the gluten chemical aggregation (Steffolani et al., 2010).

| Effects on rheological properties
Pentosanase produces a dough of greater extensibility and lower resistance to extension by interfering in protein-pentosan interactions (Primo-Martin et al., 2003). Decrease in resistance values of doughs treated with pentosanase possibly is due to the increasing amount of soluble pentosans and released amount of free water and the inhibition of gluten network formation according to glutenin-pentosan interactions (Primo-Martin et al., 2003).
Revealed a decrease in development time and dough stability in pentosanase treated flours, which leads to production of weak doughs.
Extensibility is an important factor reflecting the dough strength.
Extensibility of wheat dough is closely related to the content of dietary fiber. According to Lu et al., (2015), cellulase could degrade dietary fiber and reduce the extensibility of wheat dough. Moreover, the softening mechanism of cellulase might be attributed to the release of water that occurs when cellulose, as cellulase substrate is hydrolyzed to reduce its water binding capacity. The released water can reduce dough viscosity resulting in softer dough with better machining properties (Figure 4b; Harada et al., 2000;Zhang et al., 2019).
In contrast, according to Liu et al., (2017), the cellulase addition significantly (p <.05) increased the development time, stability, departure time, mixing tolerance index, extensibility, and stickiness of regular dough, and decreased both softening and resistance to extension.
The antistaling effect of cellulase on bread is described by Yurdugul et al., (2012). It can be noted that the cross-linking between starch-protein is in charge of bread staling. The antistaling effect of cellulase could be according to the enzyme cell wall degradation, and monosaccharides and oligosaccharides resulting from the enzyme action which cause an alteration in water distribution between starch-protein matrix (Yurdugul et al., 2012;Decamps et al., 2016;Joye et al., 2009).

| CON CLUS IONS
Adding enzymes to wheat flour has recently become a common practice to overcome the gluten deficiencies according to their impact on the properties of gluten protein and network construction through affecting its cross-linking and bonds. Beside the alteration of gluten functionality, enzyme modification is recognized as a safer and healthier method compared with chemical agents because they are inactivated following the heating process in wheat-based foods. Enzymatic hydrolysis strongly ameliorates the emulsification, solubility, foaming, and nutritive properties of gluten proteins. The final quality of bakery products significantly owes to flour composition (proteins, starch, arabinoxylan, pentosan, cellulose, etc) and their interactions. Having softening effects, hydrolytic enzymes affect flour ingredients and directly and indirectly affect gluten properties. Taken together, protease, peptidase, and alkalase directly affect gluten, while xylanase (affect arabinoxylan), pentosanase (affect pentosan), and cellulase (affect cellulose) indirectly affect gluten protein. It can be concluded that through enzyme modification, gluten characteristics can be favorably altered in wheat-based products.

E TH I C S S TATEM ENT
Human and animal testing is unnecessary in this study.

CO N FLI C T O F I NTE R E S T
The authors declare that they do not have any conflict of interest.