Bacteriophages and food safety: An updated overview

Abstract Despite significant advances in pathogen survival and food cleaning measures, foodborne diseases continue to be the main reason for hospitalization or other fatality globally. Conventional antibacterial techniques including pasteurization, pressurized preparation, radioactivity, as well as synthetic antiseptics could indeed decrease bacterial activity in nutrition to variable levels, despite their serious downsides like an elevated upfront outlay, the possibility of accessing malfunctions due to one corrosiveness, as well as an adverse effect upon those the foodstuffs' organoleptic properties and maybe their nutritional significance. Greatest significantly, these cleansing methods eliminate all contaminants, including numerous (often beneficial) bacteria found naturally in food. A huge amount of scientific publication that discussed the application of virus bioremediation to treat a multitude of pathogenic bacteria in meals spanning between prepared raw food to fresh fruit and vegetables although since initial idea through using retroviruses on meals. Furthermore, the quantity of widely viable bacteriophage‐containing medicines licensed for use in health and safety purposes has continuously expanded. Bacteriophage bio‐control, a leafy and ordinary technique that employs lytic bacteriophages extracted from the atmosphere to selectively target pathogenic bacteria and remove meaningfully decrease their stages meals, is one potential remedy that solves some of these difficulties. It has been suggested that applying bacteriophages to food is a unique method for avoiding bacterial development in vegetables. Because of their selectivity, security, stability, and use, bacteriophages are desirable. Phages have been utilized in post‐harvest activities, either alone or in combination with antimicrobial drugs, since they are effective, strain‐specific, informal to split and manipulate. In this review to ensure food safety, it may be viable to use retroviruses as a spontaneous treatment in the thread pollution of fresh picked fruits and vegetables, dairy, and convenience foods.


| INTRODUC TI ON
Dieticians and other health specialists all over the world highly advocate eating fresh fruit and vegetables because they are high in vitamins, minerals, and other nutrients (Fan et al., 2009). When compared to additional nutrition categories such as meat, fish, and dairy, fresh produce continues to be a major reason for epidemics of contaminated food sources. Since 1990, there have been over 400 emergences of foodborne infection related to produce. Fresh meals like tomato, leafy greens, other fruit and vegetables, as well as sprouted seeds like clover, mung beans, and alfalfa, are the most commonly connected with outbreaks (Murray et al., 2017). Meanwhile, better fruit is often grown on open fields, and gathered product is more prone to diarrheal disease from staff, pollen, water sources, thread liquid, predators, feces, and many other factors (Fan et al., 2009).
Between 2010 and 2018, the Centers for Disease Control (CDC) recorded 51,710 response of foodborne illness. Consuming contaminated food has been linked to 25,388 outbreaks, 12,055 of which needed hospitalization, and 950 of which resulted in fatal illnesses (Jagannathan et al., 2022). Besides causing foodborne illnesses, microorganisms may reduce the quality of vegetables, resulting in spoilage. Since fruits and vegetables include living tissue, which makes them extremely perishable and prone to loss, they must be kept alive throughout the production process and up until sale.
Food waste from farm to fork has a significant economic and environmental impact (Rawat, 2015). Post-harvest losses of fruits and vegetables can range from 30% to 40% globally, and they can be significantly higher in underdeveloped countries. Furthermore, it is anticipated that 20% of product in the US spoils each year (Barth et al., 2009). It is essential to take into account strategies for boosting productivity and decreasing waste. The need for non-chemical methods of food protection is still there due to rising organic food production and rising health consciousness. Over time, a diversity of tactics takes suggested stop contamination of fresh fruit and vegetables. In order to maintain safety via bio-sanitization and biopreservation, utilized the bacteriophages as bio regulator agents takes remained researched then put into practice which diet business. Bacteriophages, sometimes referred to as phages, are bacterial worms that may reproduce inside their bacterial hosts, lysing their cells and finally killing them. The name "bacteriophage" was initially adopted by microbiologist Felix d'Herelle after discovering this unidentified virus in samples of human feces. In order to mature and reproduce, bacteriophages are obligate intracellular parasites that require living hosts. They are among the creatures that are most common. Phages may be found anywhere; however, they tend to be more common in settings with the suitable host microbes. A number of phages that may infect starter cultures and inhibit their growth provide a constant threat to some food firms, particularly those in the dairy and fermentation industries (O'Sullivan et al., 2019).
Instead, different phages are used to stop food from rotting and from being infected with harmful bacteria, hence reducing food waste and the risk of catching foodborne illnesses. It is well acknowledged that a variety of food products post a threat to humanoid health because of pervasive microbial adulteration, which can result in disease or death (Ahmad, 2023;Usman et al., 2023). Products, fisheries, milk products, chicken, and veggies are examples of items that are routinely commercially manufactured through semi husbandry, large founder, or inter shipping. There is a greater chance of contamination as a result. It has been shown that phages may be effectively utilized to lower microbial pollution on food products, hence enhancing food safety Antimicrobials are commonly present in a variety of products, including unpasteurized milk, poultry, cheeses, vegetables and fresh fruits (Greer, 2005;Islam et al., 2022).
Numerous research has been conducted to assess how bacteriophages may be used to create antibacterial agents to enhance microbiological food safety as well as their ability to reduce pathogen and spoilage microorganisms. This review to ensure food safety, it may be viable to use retroviruses as a spontaneous treatment in the thread pollution of fresh picked fruits and vegetables, dairy, and convenience foods.

| FOODBORNE ILLNE SS LINK ED TO FOO D
Contamination is one of the leading contributors of disease and death around the globe. Roughly 250 gastrointestinal diseases were identified so far, and around 9.4 million instances of foodborne outbreaks are registered in the U.S. every year, resulting in about 56,000 hospitalizations with 1300 fatalities. The majority of these cases are caused by a specific group of food-borne pathogens such as Shigella, Salmonella, Campylobacter, Listeria monocytogenes, and Escherichia coli pathotypes other enteric microorganisms (Murray et al., 2017;Scallan et al., 2011). Because of the strong demand for fresh produce, the majority of these occurrences are linked to insufficient thermal stowage, besides microbiologic dirtied gear, but ingesting food after hazardous causes is continuously implicated (Żaczek et al., 2015). Bacteriophages are specialized viruses that attack bacteria as an alternative to antibiotics by rupturing the cell wall to address the emergence of bacterial resistance (Bragg et al., 2014).
Bacteriophages with either RNA or DNA genomes can produce endolysin enzymes, which cleave peptidoglycan to lyse cell walls.
Moreover, the genome of bacteriophages contains proteins known as amurins, which prevent the production of cell walls and cause cell walls to rupture (Woznica et al., 2015).

| PHAG E RE S E ARCH TO IMPROVE FOOD SAFE T Y
Since discovery of bacteriophage by Francis Type of circuit and Walter d'Herelle a century earlier, researchers have shown that phages can cure microbial enterococcus illnesses like cholera, correctly selected, and giardiasis in addition to a diversity of acute or prolonged pathogens in fields like cardiology, gastroenterology, cardiology, neonatology, and multiple surgeries (Wittebole et al., 2014). Since then, such infectious beings have been utilized for a variety of farming applications, as well as for animal and human uses, even though to our understanding, advertising bacteriophage therapy on local food have never been recorded (Sillankorva et al., 2012). Fresh produce-related episodes of contaminated food have highlighted the importance of practical methods to eliminate harmful bacteria from food. It has been demonstrated that traditional commercial sanitizers fail to eliminate harmful bacteria from either the skins of fruits and vegetables (Bhardwaj et al., 2015).
Radioactivity, consumable covering, nitrogen oxides, ultraviolet, climate-controlled storing, potassium permanganate, water, and sometimes perhaps viral proteins are just a few of the techniques that were researched to discover more efficient options to assure bacterial elimination on healthy produce (Mahajan et al., 2014;Islam et al., 2022). A few of the methods that have been studied to find more effective ways to ensure bacterial elimination on healthy produce include radioactivity, consumable covering, nitrogen oxides, ultraviolet, climate-controlled storage, potassium permanganate, water, and occasionally perhaps viral  Lysogenic phages inject their viral genome into the host's genetic material, whereas lytic phages kill and destroy infected host cells (Garvey, 2020). Phages with a restricted host range and improved selectivity utilized a variety of host receptors, such as proteins, sugars, and lipopolysaccharides, to bind to the host cell (Batinovic et al., 2019). When its prophase's gene is inserted by lytic bacteriophage, or chromosome, of the host bacterium, several prophages (poly-lysogenic strains) may be present (Marcó & Mercanti, 2021).

| BAC TERIOPHAG E S -E VOLUTION A S ANTIMICROB IAL S
Lysogenic phages are important for the biosphere because they regulate population size, promote biomass turnover, and release nutrients in places anywhere lysogenic species might modify in microbial communities (Braga et al., 2020).
Prior to the development of antibiotics, the antibacterial activity of phages was long regarded for the treatment of infectious diseases. The notion of employing microbes was initially supported by the microbiologists Felix d'Herelle, and phages were formally identified in 1917. D'Herelle acknowledged the phages' biocompatibility with the host patient while maintaining their selectivity and potency in eliminating dangerous germ cells. After providing mental healthy hens with Salmonella gallinarum, D'Herelle undertook many studies employing viruses and bacteria for intravascular (IV) therapy versus invading microbial illnesses. The first time that phages that might infect Shigella dysenteriae were used to treat bacillary dysentery was in 1921 (Braga et al., 2020). In India, phage therapy controlled near a significant drip in transience (62.8%-8.1%), and phages targeted at the cholera bacteria were also added to village water supplies to stop outbreaks.
Phage therapy was exceeded by the discovery and development of antibiotics in the 1930s (sylph medicines) and 1940s (penicillin).
Viral investigation has problems with unreliable findings, administration dosages, repeatability challenges, and a lack of genetic information. It is also necessary to consider difficulties such as huge composition, production, durability, including preservation (Garvey, 2020). Poland maintained its bacteriophage investigation, which has proven effective combating AMR diseases, although the United States and Continental Europe abandoned the concept.
When injected with ipratropium, bacteriophage R was shown to be less efficient versus K1 E. coli versus 8 dosages of the drug doxycycline (IM). If pathogenic organisms were not discovered in the muscular, heart, plasma, or hepatic of medicated animals 16 hours after treatment, phage clearance was obvious. Research done in Eastern Europe has shown that phages given parenterally or entirely have been effective for around 90 years without endangering the health of the patients (Tang et al., 2019). Phage treatment may be able to provide much-needed alternatives when present therapeutic methods are failing, given the obvious and worrisome threat that AMR poses. The discovery of bacteriophage treatment might give methods are usually throughout the event of Clostridioides difficile infection (CDI), an MDR microbial sickness with a significant fatality rates. In the United States, the United Kingdom, the Republic of Poland, Belgium, and Georgia there are presently six medical centres that provide bacteriophage for the management of contagious disorders (Selle et al., 2020).

| BAC TERIOPHAG E S FOR B IOCONTROL OF PATHOG EN S IN FOOD
Microbe pesticide assays get the ability to significantly enhance bacterial quality because to their long tradition of healthy use, comparatively straightforward processing, and powerful and targeted antibacterial, as indicated in Table 1 and Figure 1.

| Bacteriophage to control Listeria monocytogenes contamination
The identification and eradication of L. monocytogenes is crucial to guaranteeing the chain's safety, particularly in RTE meals, since it may live and develop at the normal low level (2-8°C) employed numerous foods throughout their storage and distribution.
Accordingly, several studies have demonstrated that using bacteriophages on a variety of foods, notably RTE meals, is beneficial for lowering L. monocytogenes infection. As contrasted to vanillin and salt lactose there at ambient usage level of 6-8°C, a commercialized monophase preparations (i.e., phage preparedness comprising about one solitary bacteriophage) against Listeria was demonstrated to be higher successful in reducing the concentrations of L. monocytogenes in sliced ham (Selle et al., 2020). The same monophage preparation was demonstrated to be efficient in reducing L. monocytogenes on the superficial of various deli meats. Roast beef and turkey slices were frozen at 4 and 10°C, respectively. The Listeria specific phage not only acted as a stand-alone L. monocytogenes inhibitor but also improved the efficiency of other antimicrobials when coupled with potassium lactate or sodium diacetate (Chibeu et al., 2013).
All of these research utilized the same bacteriophage preparation. A realistic way to include a larger variety of mark species also reduce the possibility that resilient microorganisms would arise is to combine numerous bacteriophages to produce a "phage cocktail." In certain goods that had been intentionally dirtied through L. monocytogenes, such as lettuce, cured salmon, Gali apples pieces, and hard pasteurized cheese, the levels of the pathogen decreased by 0.7-1.1 logs when the technique was applied. The effect of the L. monocytogenes-specific cocktail on pre-made, freezer meals was investigated in the same experiment. Cycles of freezing and thawing, phage cocktail treatment, and an experimental L. monocytogenes infection were all applied to the meals. The findings revealed a 2.2 log decrease in L. monocytogenes, indicating possessing of bacteriophage might be a useful strategy for reducing L. monocytogenes in food below "storage abuse" circumstances, such as frequently defrosted throughout stowage, either intentionally or unintentionally (Perera et al., 2015).

| Bacteriophage to control Salmonella contamination
The number of Salmonella strains that are susceptible on overall exteriors examined by 2-4 logs was significantly reduced by a cocktail of Salmonella specific bacteriophages, according to recent TA B L E 1 Application of bacteriophages in food safety and reported outcomes.

Reported results References
Listeria monocytogenes Elimination of cut melon and apples. combination with nisin. At 4°C, there is no combination phage-nisin activity in beef. elimination from soft cheese with a surface-ripened red stain Garcia et al. (2008) Salmonella spp.
Salmonella counts on melon slices dropped by ~3.5 logs at 5 and 10°C and ~2.5 logs at 20°C following the application of a four-phage cocktail, but there was no bacterial reduction on apple slices after phage administration Whichard et al. (2003) Escherichia coli Decrease in drinkable water and rectal administration (cattle) Raya et al. (2006) Shigella sonnei A five-phage, Shigella specific cocktail was administered to a variety of RTE meals, such as smoked salmon, lettuce, melon, corned beef, and pre-cooked chicken, which decreased the recovery of Shigella ~ 1.0-1.4 logs when matched to control Soffer et al. (2017) Campylobacter Reduced cecal concentration counts (broilers) Wagenaar et al. (2005) F I G U R E 1 Bateriophage endolysin for food safety application.

| Bacteriophage to control E. coli contamination
According to recent investigations, fresh vegetables, fresh milk polluted with E. coli, and (UHT) preserved milk have all been effectively treated utilizing E. coli-specific phage formulations. One phage dramatically abridged amount of E. coli O157:H7 on spinach leaves and green pepper slices through around 1-4 logs in the initial research.
The initial decline persisted at 4°C, but there was significant regrowth on 25°C. When two or three phages were coupled in the retrial, the prevalence of E. coli was reduced to extremely low levels in both UHT and unpasteurized milk. In contrast to samples treated with the two-phage cocktail, where it started to develop again, the E. coli strain continued to diminish in all samples treated with the three-phage preparation during storage at both 4 and 25°C. Despite the fact that the fundamental details are not completely tacit, the three-phage cocktail probably managed resistance better than a two-phage cocktail (Tomat et al., 2014). In the past, multi-phage cocktails have been proven to be more effective. The underlying causes of this phenomenon have not been thoroughly investigated.
This idea is quite similar to the multi-hurdle method, which suggests combining various antibacterial tactics to prevent the emergence of bacterial resistance (Snyder et al., 2016).

| Bacteriophage to control Shigella spp. contamination
Currently, the FDA has only authorized one food safety phage preparation that exclusively targets Shigella spp. It was awarded the GRAS certification in 2017 (GRN 672) for this five-phage cocktail after it remained exposed that the heights of Shigella were decreased through around 1 log in a variety of foods, including lettuce, yoghurt, smoked salmon, deli corned beef, melons and chicken breast meat.
In a different study, the same combination of Shigella specific bacteriophages was used to assess the efficacy of giving phages against pharmaceuticals to mice exposed to a Shigella sonnei strain (Soffer et al., 2017). The results of the above research showed that although the Shigella-specific phages concoction was similarly efficient as just a basic antimicrobial at lowering the amount of bacteria in mice, antimicrobial therapy substantially altered the uniqueness of the cursor bowel society, so although viral diagnosis was doing not. Retroviral management thus was much fairly mild consequence just on mice's regular gut bacteria than antibiotic therapy was doing. The researcher's observations show that the phage had no negative effects on the mice's weight, morbidity, mortality, or any other physiological characteristics. Both the mice's blood and urine contained the same elements as before (Mai et al., 2015).

| Bacteriophage to control Campylobacter contamination
Prior to the discovery of antibiotics, efforts were made to treat patients using bacteriophages. Bacteriophages have two life cycles, lysogenic and lytic, which may be used in therapeutics. Bacteriophages are characterized by their specificity, which allows them to selectively work against certain bacteria while having no negative effects on the surrounding flora, which is crucial for the advancement of human health. On the other hand, this uniqueness causes certain issues with phage therapy's immunity problem and also necessitates very specialized techniques (Bragg et al., 2014;Hashempour-Baltork et al., 2019;Lin et al., 2017). The potential to decrease Campylobacter adulteration of numerous foods has been investigated for some of the Campylobacter bacteriophages that have been inaccessible from chickens, counting their feces and the surface and internal tissues of their livers (Hammerl et al., 2014). For instance, Hammerl and colleagues discovered a significant reduction (3 logs) in Campylobacter fecal levels when two phages were delivered consecutively to 20-day-old chicks (a Group III phage, then a Group II phage). It's noteworthy to note that the Group III phage was unsuccessful when given unaided or in combination through additional Group III phage, signifying that a mix of Group II and III phages was obligatory for maximal efficiency. Campylobacter-specific phages have previously been isolated from a small number of Campylobacter isolates, with many investigations using the single isolate of C. jejuni NCTC 12662 as the host strain. The discovery of several Group III phages, the majority of which target the capsular polysaccharide, a specific receptor, was made possible by that one strain. On the other hand, Group II phages that enter through the flagella are often seen on C. jejuni RM1221 (Sørensen et al., 2015).

| BAC TERI OPHAG E S AT THE P OS T-HARVE S T S TAG E OF FOOD PRODUC TI ON
Depending on the amount of preservatives used, the nutrient-rich conditions found in food may promote the survival and emergence of several bacterial illnesses. According to the research, using certain phages can help prevent the spread of a number of hazardous diseases. In order to manage infections in postharvest food products, several have outlined intervention procedures that use phages as illustrated in Table 2.

| Meat
As part of ongoing research on meat systems, researchers done a thorough inspection of the Intralytix phage cocktail EcoShield PXTM with a focus on E. coli that generates Shiga toxin. In eight various dietary commodities, comprising meat chuck roast, beef mince, chicken breast, cooked chicken, salmon, cheese, cantaloupe, and mustard greens, they discovered that such microbes were effective in reducing pathogen levels (at 3.0 log CFU/g). 97% of the foods were analyzed, and the reductions in E. coli O157:H7 were substantial (p .05) once the phages remained administered at 5106 and 1107 PFU/g.
When usual levels of E. coli (1-10 CFU/10 g) were found in beef parts that were offered for sale, pathogen counts were reduced by 80% (Vikram et al., 2020).
In another study, scientist evaluated the efficacy of four ) After 5 days, a log decrease of 1.5 CFU/g was noticed Lone et al. (2016) Cabbage Salmonella enteriditis (10 4 CFU/mL) PA13076, PC2184 (104) After 5 h, a log decrease of 3.0-3.86 CFU/sample was noticed Bao et al. (2015) These findings are in contrast to those obtained using the commercially available SalmoFresh™ (manufactured by Intralytix Inc.), which reduced the levels of Salmonella in infected chicken breasts by up to 1.5 logs (Duc et al., 2020). Researchers conducted research on beef meat to evaluate the effectiveness of a phage cocktail against two Shiga-toxigenic E. coli strains and the clinically important Entero-pathogenic strain. They also examined the results of similar tasks carried out in sterile milk and broth. However, they did note that while trials were effective when conducted at 24 and 37°C, they were less effective at 4°C (Tomat et al., 2018).

| Processed foods
There has been significant advancement in the creation of novel, FDA-approved phage therapies for a variety of critical disorders.
These include E. coli O157:H7, Shigella spp., Salmonella spp., and Listeria monocytogenes. More evidence in favor of using phages for food safety may be found in the following list of recent studies.
Scientists discovered an anti-Listeria SH3-3 phage in a food processing plant and assessed the efficiency of biocontrol against L. monocytogenes in both salmon and orange juice (Zhou et al., 2020).
Researchers demonstrated the anti-Salmonella Enteritidis phage SE07's biocontrol ability in a variety of retail products, such as fruit juice, fresh eggs, beef, and poultry. They also found that fruit juice and fresh eggs had a 2-log decrease in the germs following a 48-h test at 4°C. All of the aforementioned research strongly supports the continuous creation of phage products with a wide host range in light of the different potential applications (Thung et al., 2017).

| Strength and limitations of phage as antimicrobial
Additionally, since diverse bacterial strains typically inhabit illnesses, this specificity presents a challenge. Even though a small number of trials verified the safety of taking phage orally, the crucial concern is proper phage translocation throughout the intestinal epithelium. Studies have clearly shown that this translocation can benefit the body by controlling the immune response to native microbial antigens by preventing the development of tumor necrosis factor, interleukin-2, and interferon gamma. Nevertheless, other research did not find an appreciable rise in cytokine levels following phage therapy. Despite the paucity of information about phage treatment, studies have shown that it has far less side effects than conventional antibiotics, in addition to reducing the gut pathogenic flora. Finding the phages with the best infectivity against the target pathogen was made possible by regional specificity. This can be more beneficial when searching for phages for bacteria that are resistant to antibiotics, particularly in hospitals. Moreover, phages include enzymes like extracellular polymeric components depolymerize that may break down bacterial biofilms and extracellular polymeric materials, while antibiotics are unable to cure infections caused by bacteria that form biofilms.

| CON CLUS ION
Fresh fruit and vegetable contamination remains a major issue despite recent improvements in food safety procedures. Pathogenic bacteria and rotting bacteria can both lower product quality and increase food waste, respectively. According to recent research in the field of food microbiology, bacteriophages are particularly effective at preventing the formation of dangerous bacteria on fresh vegetables. Bacteriophage usage can be beneficial at several stages of the food production chain. Despite advancements in food safety, foodborne infections remain an issue, especially for those with weakened immune systems, such kids, the elderly, and expectant mothers.
Bacteriophage biocontrol is a viable supplementary tool in a multipronged strategy to stop the spread of foodborne infections. This strategy shows the most promise once food mainframes try toward retain the normal, then frequently favorable, bacterial community of nutriments then toward solitary eliminate germs that might reason