Optimization of spicy red pepper paste (Awaze) formulation by D‐optimal mixture design

Abstract The aim of this study was to produce spicy red pepper paste (Awaze) by addition of various sources of antioxidant, mineral, and fiber and optimize better processing methods of the paste. For this purpose, D‐optimal mixture design was used, Awaze considering color value (a*), antioxidant content, mineral content, fiber content, chewiness, and viscosity of the paste as the main parameters. Various properties of the optimized formulation were evaluated. The optimal formulation contained 65.66% red pepper, 10% garlic, 19.08% red onion, and 5.25% ginger. In the optimized formulation, the redness value (a*) increased by more than 3.12 times compared to the control with the average antioxidant activity (44.6%). The optimal formulation had a higher fiber content, chewiness, and viscosity value compared to control which is probably related to the ingredient proportion difference. Due to the higher nutritional and processing quality obtained, this formulation can be suggested for commercial and household producers as a guide to manufacture Awaze. The results obtained indicate that it is possible to production of Awaze with high nutritional value and improved processing quality by utilizing a blend of red pepper, garlic, red onion, and ginger. Therefore, this formulation stands as a viable recommendation for both commercial enterprises and household producers due to its demonstrated ability to yield Awaze with enhanced nutritional content and superior processing quality.

acid bacteria isolated from Awaze evaluated in vitro were reported by Tigu et al., 2016 andDessalegn &Ashenafi, 2010.Mixture design has been applied as an important method for the development and optimization of foods.When several food ingredients are mixed, it is difficult to analyze foods by other available methods, determining their mixing ratio can be difficult.In a food product with several mixed ingredients, a mixture experiment design is used to identify ingredients that affect the product quality and to determine the optimal mixing ratio that maximize or minimize the response level of the ingredients (Jang et al., 2011).An optimal mixture design is proved as an efficient tool to determine the best ingredient combination in food formulation.This method is widely used to find the optimal formulation in various food products by considering the best food characteristics (Al Hagbani & Nazzal, 2018).
Many Ethiopian women at household level use various proportions of red pepper, garlic, red onion, and ginger during the preparation of Awaze.There is lack of scientific information about the optimum proportion of the main ingredients that are used for the preparation of Awaze at household and commercial level.
Therefore, this study was conducted to evaluate the a) possibility of producing a new functional Awaze by increased antioxidant, ash, and fiber content and b) the physicochemical properties, nutritional content, and texture profile of enriched Awaze.To the best of our Knowledge, this is the first study in the literature focused on the production of Awaze designed for consumers by the combination of red pepper, garlic, red onion, and ginger.

| Materials
Dried red pepper sample was collected from Bure district in September, 2022, Amhara region, Ethiopia, the place is known for its high volume production of red pepper as described in Dessie et al., 2019.Matured red onion, ginger, and garlic were obtained from a vegetable improvement center, Holeta Agricultural research center.The other ingredients (spices), cardamom, fenugreek, clove, cinnamon, kemune, long pepper, gewze, coriander, thyme, basil, white cumin, black cumin, rue, rosemary, and ground pepper were purchased from Holeta spice market in September, 2022, Oromia region, Ethiopia.The collected samples were transported to Holeta Agricultural research center, Food science and Nutrition research laboratory for Awaze paste product development.
Analytical grade chemicals and distilled water have been used in various steps of Awaze paste preparation and physicochemical quality analysis.

| Experimental design
Maximum and minimum levels of independent variables were first investigated by doing a preliminary survey at various household-made Awaze paste producers in northwest Amhara, areas where the product is frequently produced and consumed.Based on the survey of the raw materials (ingredients) that are used in the preparation of Awaze paste, the ingredients that were used in a higher proportion were red pepper, red onion, garlic, and ginger.It was found that a maximum of 30% garlic, 20% red onion, and 10% ginger will be substituted with red pepper.
It was found that substituting more than 30% garlic and 10% ginger to make Awaze paste results in a color change, whereas mixing more than 20% red onion decreases the shelf life of Awaze paste.
Accordingly, a total of 15 formulations were generated using D-optimal mixture design with four ingredients (red pepper, garlic, red onion, and ginger) using Design-Expert 13.0.1(Stat-EaseInc.) software which was used to define the optimum proportion of the enriched Awaze paste formulation.In this study, D-optimal design was used with four components: red pepper, garlic, red onion, and ginger.Table 1 displays the composition of each blend calculated from the experimental design.The amount of the components was selected based on preliminary survey result of household producers of Awaze paste (red pepper: 60%-90%, garlic: 10%-30%, red onion: 5%-20%, and ginger: 5%-10%).Design-Expert software designed 15 samples (Table 1).Effect of red pepper, garlic, red onion, and ginger on the attribute of Awaze paste was investigated and the optimum mixture was selected.Formulated Awaze paste and control sample were compared based on selected parameters for Awaze paste, including color (a* value), viscosity, antioxidant activity, total ash content, fiber, and chewiness of the product (Tables 1 and 2).

| Control (household made) sample
At household-level preparation (unknown formulation), the ingredients used are the same for all districts in the region for Awaze paste preparation but the ratio of the ingredient used is different.In order to optimize the difference in the mixing ratio, the experiment is conducted, and the area which is popular for the utilization of Awaze paste is selected and used as a control sample to compare to the current experiment.Household-made Awaze paste (unknown formulation) collected from Bure district, northwest Amhara, areas where the product is frequently produced and consumed or utilized, was used as a control sample.

| Optimization and contour plots
The optimal ingredient mixing ratio was designated and the experimental points predicted by the numerical optimization and graphical optimization of ingredients in the mixture.For simultaneous optimization each response must have a low and high value assigned to each goal.On the work sheet, the 'goal' field for response must be one of 5 choices: 'None', 'maximum', 'minimum', 'target', or 'in range.
Factors will always be included in the optimization.For graphical optimization, the minimum and maximum of each response were determined and contour plots were super-imposed within the possible ranges, after which the best area was selected.

| Drying, roasting, and milling of ingredient
The preparation of Awaze paste was preformed following the traditional household-made method as described in (Idris et al., 2001).The ingredients collected for Awaze paste preparation were dry spices and wet spices.Spices are classified as dry spices, such as red pepper, cardamom, fenugreek, clove, cinnamon, kemune, long pepper, coriander, white cumin, black cumin, and ground pepper, and as wet spices such as red onion, garlic, ginger, thyme, basil, rue, and rosemary.The processing of Awaze paste ingredients was preformed following the traditional methods described below.The dried red pepper was gently pulverized with a wooden mortar and pestle.Fresh un-dried garlic, red onion, and ginger were peeled and washed.A small proportion of other weight spices, such as thyme, basil, rue, rosemary, was powdered separately using a wooden mortar and pestle.The powdered wet spices were sun dried by thinly spreading on a clean surface.
Dry spices were lightly roasted and mixed with dried wet spices following the formula.The composite blends (independent variables) that contained red pepper, garlic, red onion, and ginger were prepared using the formula shown in Table 2. Controlled variables that include 5 g cardamom, 5 g fenugreek, 2.5 g white cumin, 2.5 g basil, 1.25 g black cumin, 1.25 g mekelesha, 1 g rue, 0.5 g coriander, 0.4 g rosemary, 0.4 g thyme, 20 g salt were added constantly for each formulation.The mixed spices were milled using laboratory hammer mill (Perten Instruments, Finland).Then, Awaze paste was prepared by mixing 100 g composite powder with 150 mL of boiled water in a 400 mL capacity screwcap bottle until it acquired a thick consistency as shown in (Figure 1).Control sample (household made) was collected from Bure district, Amhara region, areas where the product is frequently produced and consumed to compare with the formulated Awaze paste.

| Total ash and crude fiber content
Total ash content and crude fiber were determined according to the association of official analytical chemists method (AOAC, 1990) no.

| Viscosity
A 3 g Awaze paste sample was dissolved in 5 mL distilled water, and the viscosity of the solution was determined using Brookfield (model Lv-3, Middleboro, MA 02346, USA) set at 60 rpm and a temperature of 25°C (Rahman, 1986).

| DPPH radical scavenging activity
Methanolic extracts were prepared as reported previously by (Luthria et al., 2006).400 mg of Awaze paste sample was placed in a 15 mL centrifuge tube with 5 mL of the solvent mixture MeOH: H 2 O (80:20, % v/v).The vials were then placed in a sonicator bath at 25°C temperature for 30 min.The mixture was centrifuged and the supernatant was transferred into a 10 mL volumetric flask.The residue was re-suspended in 5 mL of MeOH:H 2 O (80:20, %v/v), gently mixed manually and sonicated for an additional 30 min followed by centrifugation.The supernatant was combined with the initial extract and the volume of combined supernatant was made up to 10 mL with the extraction solvent and appropriate aliquots of extracts were filtered and assayed for antioxidant activity.Antioxidant activity was evaluated by measuring the radical scavenging effect of spicy red pepper paste (Awaze) sample methanolic extracts toward the 2,2-diphenyl-1-picrylhydracyl (DPPH) as reported previously by (Sharifi et al., 2015).Five mL of a 0.1 mM methanol solution of DPPH was added to 20 μL of methanol extracts of Awaze paste samples.The tubes were allowed to stand at 27°C for 20 min.The decrease in absorbance at 517 nm was recorded in a spectrophotometer (Shimadzu UV-vis spectrophotometer 1900).
Radical scavenging activity was expressed as inhibition percentage and was calculated using the following formula: (1) F I G U R E 1 Processing steps for the preparation of Awaze.

| Mineral content
The mineral contents (Fe, Zn, K, Ca, and Mg) of the formulated sample and control sample were determined by atomic absorption spectrophotometer (Model: Agilent serious, America) by using the method (AOAC, 1990).A sample (0.5 g) was digested by using a muffle furnace at 550 degree centigrade for 5 hours until the residue ash became white.The ash sample was digested by using concentrated HCl.The digested sample was diluted up to 100 mL for mineral analysis.
where TV=Volume marked by 100 mL volumetric flask, df = dilution factor.

| Texture profile analysis (TPA)
The texture profile of Awaze paste was measured using a Texture Analyzer (model: TA.XT.Plus) (Stable Micro Systems, UK), based on the method described in Chen and Rosenthal ( 2015)) using P-2 (cylindrical probe having 2 mm diameter) with pre-test speed, and the test speeds were stated as 2,1, 2 mm/sec, respectively.Compression was a distance mode of 5 mm and load cell was 50 kg.Two compressions in a raw were applied on the samples.The test produced the hardness, adhesiveness, cohesiveness, springiness, gumminess, and chewiness of Awaze paste was measured.The texture profile of Awaze paste was determined using the following approaches: Hardness (N) is the maximum height of the first peak on the first compression.
Adhesiveness (N.s) describes the work for overcoming the force of attraction between the area of samples coming in contact with each other.

Cohesiveness is the ratio of the second compression to the first compression positive area
Springiness is measured by dividing the distance of the detected height of the product on the second compression by the original compression distance.
Gumminess (N) is the product of hardness and cohesiveness (strength required to chew dawn).
Chewiness (N) is the product of gumminess and springiness (measure of the energy spent in the chew down process) as per the procedure of (Cardoso et al., 2009).

| Data analysis
All the formulation values were presented as the mean ± standard deviation.Statistical analysis was performed using SPSS software (IBM, Chicago, IL, USA).One way analysis of variance and Duncan multiple range test (IBM SPSS Version 24) were used to explore the significant difference between the formulations with 95% confidence interval at (p < .05).Correlation coefficients were analyzed using Minitab software (Version 17).Numerical optimization was employed using statistical software Design-Expert 13.0® from Stat-Ease Inc. with criteria of maximum red onion while red pepper, garlic, and ginger were kept in range.The contour plot for the selected variables was constructed to determine the best formulations for the chosen response.

| Effect of red pepper, garlic, red onion, and ginger proportion on color value of Awaze paste
The surface color of Awaze was measured using a colorimeter and is presented in Table 4.There is a significant difference at p < .05 in L*, a*, b*, chroma, and hue angle for the formulated paste samples.
The color values of lightness (L*), redness (a*), and yellowness (b*) of the products and control sample were in the range of 5.3-19.9,5.85-23.4,and 4.15-28.1,respectively.Color is an essential food quality for consumer acceptability and it is highly dependent on the ingredient types used.The degree of redness (a*) was found to have a positive value ranging from 5.85 to 23.4.The difference in color value of the formulated paste could be attributed to the differences in mixing ratio of the powders.The degree of color value difference in the paste sample is affected by the type of processing, packaging material, and storage temperature (Mo et al., 2015).Following an analysis of the 15 experimental results, the redness value (a*) reached a maximal value of 23.4 for a mixing ratio of red pepper, garlic, red onion, and ginger of 74.39%, 13.3%, 5.0%, and 7.28%, respectively, whereas the control sample (household made) had the lowest value (5.85).The results revealed that the combination of red pepper, garlic, red onion, and ginger increased the redness (a*) value of Awaze paste by up to 4 times compared to the control sample.
The low value of the control sample could be due to the long storage period of the product.A previous study on fermented red pepperbased products revealed that the color value is associated with the microbial composition, which metabolizes the complex and converts it into simple molecules responsible for a unique taste, aroma, and color (Yang et al., 2018).The color values of Awaze paste that was obtained in this study were in agreement with the study that was conducted on another red pepper-based paste product (gochujang) (Ramalingam., 2022).Similar redness (a*) value for Korean red pepper paste was reported (15.13-31.34)(Oh et al., 2011) (Park et al., 2010).Generally a higher value of a* indicates the degree of redness color of Awaze paste which is a criteria for best quality product.
The contour plot In Figure 2 indicated that with increasing the mixing ratio of red pepper and red onion, the degree of redness was significantly increased.In contrast, an increase in garlic and ginger proportion decreased the color value of Awaze paste.The results showed the redness of Awaze paste increases with an increase in mixing ratio of red pepper and red onion.

| Effect of red pepper, garlic, red onion, and ginger proportion on ash content of Awaze paste
As the ash content analysis showed (Table 5), red pepper, garlic, red onion, and ginger had a strong effect on the ash content of Awaze paste.The ash content of Awaze paste samples showed significant difference for the formulated paste samples (p < .05)(Table 5).The ash content gives an indication of the mineral composition of food materials.In this study, the ash values of the formulated Awaze paste and control sample ranged 6.32%-9.94%.At a mixing ratio of 68.8% red pepper, 16.1% garlic, 5.02% red onion, and 10% ginger, the ash content was the highest.The increase in the ash content may be due to the high amount of red pepper and ginger because red pepper and ginger are a good source of minerals compared to garlic and red onion (Kefale et al., 2023), (Table 3).The values in this study were lower than the reported values for commercial red pepper paste (5.1%-21.1%)(Park et al., 2017).However, lower ash content (2.83%-3.1%)was also reported for red pepper paste that was prepared using a different milling technique (Bankole et al., 2013).
With an increase in the mixing ratio of red pepper and garlic, the ash content of the product was increased (Figure 3).In contrast, increase in red onion and ginger significantly decreases the ash content of the paste, it may be due to the low ash content of red onion (Table 3).

| Effect of red pepper, garlic, red onion, and ginger on crude fiber content of awaze paste
The blending ratios resulted in a significant change (p < .05) in fiber content.The crude fibers of the formulated Awaze paste varied from 16.86 to 29.06% (Table 5).The crude fiber content of red pepper, garlic, red onion, and ginger (individual samples) was 38.77%, 3.03%, 3.87%, 6.62%, respectively, as shown in Table 3.The maximal values for fiber content (29.06%) were found for formulation with 76.39% red pepper, 10% garlic, 8.6% red onion, and 5.01% for ginger, whereas the lowest fiber content (14.6%) is identified in Awaze paste from 70.13% red pepper, 10% garlic 9.87% red onion, and 10% ginger.The variation in the fiber content of the formulations is due to the difference in the blending ratio of the ingredients (spices).
The crude fiber content found in this study was higher compared to the reported values of sweet red pepper paste(3.61%)(Dimassi et al., 2019).The crude fiber content of formulated Awaze paste was The effect of blending ratio on color value (a*) of spicy red pepper paste (Awaze) obtained using actual components as shown by contour plot.
TA B L E 3 Physicochemical and Textural properties of red pepper, garlic, red onion, and ginger samples.higher (16.86%-29.06%)than study report on cheese supplemented sweet red pepper paste (Atwaa et al., 2020).Based on the contour plot, the fiber content of Awaze paste was increased with an increase in mixing ratio of red pepper, red onion, and ginger as shown in Figure 4.Therefore, higher dietary fiber content is similarly expected with an increasing ratio of red pepper, ginger, and red onion used in this study, which have higher fiber content and contributed for higher fiber as their proportion increased in the formulation.

F I G U R E 3
The effect of blending ratio on ash content of spicy red pepper paste (Awaze) obtained using actual components as shown by contour plots.
TA B L E 5 Effect of red pepper, garlic, red onion, and ginger on physicochemical property of formulated spicy red pepper paste (Awaze) and control sample (household made).Abbreviations: FM, formulations; HF, (household-made formulation); RSA, free radical scavenging activity.

| Effect of red pepper, garlic, red onion, and ginger proportion on viscosity of Awaze paste
The result indicated that the viscosity of the formulated Awaze paste was significantly different at p < .05(Table 5).Viscosity is an important parameter for texture of food products including food and condiment pastes.The viscosity of the formulations and control sample was in the range of 71-125.5 cps.The maximal viscosity was found to be at a mixing ratio of 64.55% red pepper, 15.46% garlic, 10.17% red onion, and 9.82% for ginger.Besides, the minimal values were found to be 63.27%, 24.68%, 7.03%, and 5.02% in the corresponding order.This could be due to the relatively higher ratio of ginger and red onion because ginger and red onion had higher viscosity compared to red pepper and garlic (Kefale et al., 2023) (Table 3).
The viscosity values found in this study are lower compared to the reported values of onion paste (396-398 cps) (Naqash et al., 2021).
Also, the lower viscosity (13.24-85.84cps) was reported for hot pepper-soya bean paste at different solid contents and temperatures (Yo, 2001).A previous study reported the viscosity of salad dressing in the range of 14.3-18.1 cps (Koc, 2020) which is lower compared to the current result.It may be the difference in ingredient types used.

| Effect of red pepper, garlic, red onion, and ginger on antioxidant activity of Awaze paste
The blending ratio resulted in a significant change at p < .05 in the antioxidant activity of the formulated Awaze paste.Total antioxidant activity is an important parameter in establishing the health functionality of spices and vegetable product.The antioxidant activity of the formulated product (free radical scavenging activity towards DPPH) was observed at 20 μL concentration (Table 5).The antioxidant activity (free radical scavenging activity towards DPPH) was in the range of 34.24%-54.13%(% inhibition) for all formulations at a concentration of 20 μL.The highest antioxidant activity of 54.13% was found for formulations that contained 60% red pepper, 20.2% garlic, 12.01% red onion, and 7.77% ginger.The lowest antioxidant activity of 34.24% was found for formulations that contained 68.84% red pepper, 13.99% garlic, 12.12% red onion, and 5.01% ginger, it may be due to the relatively low proportion of garlic in the formulation.
This report is in agreement with the study that reported an antioxidant activity in the range of 30%-90% for different spices at different concentrations (Sasikumar et al., 2020).A previous study obtained an antioxidant activity of hot and sweet red pepper paste in the range 40%-90% (Sayin & Arslan, 2015), which is higher than the result of the current study.A previous study reported on DPPH activity of soya bean-based red pepper paste in the range 31.88%-43.19% on different fermentation times (Yang et al., 2018), which is lower antioxidant activity content compared to the current result.
As shown in Figure 5, as the mixing ratio of red pepper and ginger increases, the percentage of free radical scavenging was significantly increased.As the mixing ratio of red onion was increased, the percentage of free radical scavenging was decreased.The percentage of free radical was increased with an increase in the ratio between garlic and red pepper.Therefore, we suggest that a significant difference of antioxidant activity Awaze pastes is due to the difference in mixing ratio of ingredients.

F I G U R E 4
The effect of blending ratio on fiber content of spicy red pepper paste (Awaze) obtained using actual components as shown by contour plot.

| Effect of red pepper, garlic, red onion, and ginger proportion on mineral contents of awaze paste
The result indicated a significant change in mineral contents (Fe, Zn, K, Ca, and Mg) for the formulations and control sample (p < .05)(Table 7).The formulated spicy red pepper paste (Awaze) had mean values of Fe, Zn, K, Ca, and Mg in the range of 36.54-108.82, 1.72-26.93, 913.34-2783.32,28.09-92.27, and 73.48-198.6 mg/100 g, respectively.Formulation of 76.39% red pepper, 10% garlic, 8.6% red onion, and 5.01% ginger had highest Fe and Zn content of 108.82 and 26.93 mg/100 g, respectively, this may be due to the high proportion of red pepper and red onion.Formulation of 63.27% red pepper, 24.68% garlic, 7.03% red onion, and 5.02% ginger had highest K content with the value 2783.32 mg/100 g that is due to the high proportion of red onion.The control sample had higher Ca content 92.27 mg/100 g and lower K content (913.4 mg/100 g) and Mg content (73.5 mg/100 g) compared to the formulations of this study.
At a mixing ratio of 60% red pepper, 10.13% garlic, 19.87% red onion, and 10% ginger, the concentration of Mg had the highest value (198.68 mg/100 g).A previous study on red paper paste reported Fe content in the range of 13-35 mg/100 g and zinc content in the range of 3.3-10 mg/100 g (Park et al., 2017) which is lower than the results of the current study.These findings imply Fe and Zn content in the formulated paste is a good input for the food industry that produces condiment-based paste products.
3.6.1 | Correlation between ash content, Fe, Zn, K, Ca, and Mg contents of the formulations of Awaze paste According to Pearson correlation test, Table 6 demonstrates the correlation coefficients between ash content and minerals (Fe, Zn, K, Ca, and Mg).Highly significant correlation coefficients were found between K and Mg (r = .843),Fe and Zn (r = .66)(p < .05).No statically significant positive correlation coefficient was found between ash content and Fe (r = .133),ash content and K (r = .105),Fe and K (r = .189),Zn and K (r = .086),Fe and Ca (r = .28),Zn and Ca (r = .306),effect on the textural property of Awaze paste.Hardness value of the formulation with 60% red pepper, 14.81% garlic, 15.2% red onion, and 9.9% ginger had the highest value (55.09 g) that was due to the highest proportion of garlic and ginger.Cohesiveness indicates the strength of the internal bonds in making of Awaze paste.
As seen from Table 6, cohesiveness of the formulation was in the range of 0.40-0.78.The highest cohesiveness score was obtained for formulation with 67.28% red pepper, 19.37% garlic, 8.28% red onion, 5.05% ginger which contained relatively high proportion of red pepper and garlic.
The range of springiness score of the formulations was 0.22-1.5.Formulation with 60% red pepper, 15% garlic, 20% red onion, and 5.0% ginger had the highest springiness score (1.5) that corresponded to high proportions of red pepper and ginger during preparation of Awaze paste.Gumminess score of the formulation were in the range of 5.23-17.32g, whereas the highest gumminess value was obtained for formulation with 63.27% red pepper, 24.68% garlic, 7.03% red onion, and 5.02% ginger which was prepared from high proportion of red onion and ginger.Chewiness is a function of hardness.As seen from Table 6, chewiness score of formulation with 60% red pepper, 15% garlic, 20% red onion, and 5% ginger was the highest (17.62 g).
The current result of hardness, cohesiveness, gumminess, springiness, and chewiness had lower value compared to the reported value on freeze dried fermented chili paste (Man et al., 2022).The freeze drying process influences the texture profile of the product.It is because the lyophilization of fermented chili powder was extremely dry, whereas fermented chili paste has a humidity of approximately 90%.A previous study report on the texture profile for miser paste observed higher values (Ergönül, 2013), compared to the current result.Generally, interaction between red pepper, garlic, red onion, and ginger had an effect on the texture profile of Awaze paste.

| Model development
Independent and dependent variables were fitted to linear, quadratic, and special cubic models (Table 9).To check goodness of model fit, residual plots were created.The best model has low standard deviation, high predicted R-squared (R 2 ), low predicted sum of square, and lack of fit (p > .05)(Nikzade et al., 2012).Linear model was found to be the best fit for ash content and chewiness.Quadratic model was found to be the best fit for color (a*) and free radical scavenging activity.Special cubic model was found to be the best fit for fiber content (R 2 = .98)(Table 9).

| Optimization
An optimization of Awaze paste formulation was done using the method by Derringer & Suich, 1980.The optimization was done to develop a high-quality Awaze paste with the selected important parameters, including color (a* value), antioxidant activity,

TA B L E 9
The predicted models to experimental data in D-optimal mixtures design for the dependent variables of spicy red pepper paste (Awaze).

TA B L E 1 0
Predicted and verified values of spicy red pepper paste (Awaze) optimized formula.

Note:
Data are means ± standard deviation of the replicates.Values with different lowercase letters in the same column are significantly different (LSD, p < .05).

Fe
and Mg (r = .27),Zn and Mg (0.206) (p > .05).No statically significant negative correlation coefficients were found between K and Ca, Ca and Mg, ash content and Zn, Ca, Mg content of the formulated Awaze paste (p > 0.05).Depending on several variables, such as raw materials, processing condition, and formulation difference which interact with each other, because of all these interaction factors affecting the correlation of ash content and mineral content of the formulated Awaze paste.3.7 | Effect of red pepper, garlic, red onion, and ginger proportion on texture profile of Awaze pasteThe textural property of Awaze paste is shown in

TA B L E 1
Proportions of ingredients used for the optimization of Awaze paste formulation (100%).
Effect of red pepper, garlic, red onion, and ginger on color values of spicy red pepper paste (Awaze) and control sample (household made).Data are means ± standard deviation of the replicates.Values with different lowercase letters in the same column are significantly different (LSD, p < .05).
(Kefale et al., 2023)2023).Values with the same letter in the same row are not significantly different at p<0.05.Color(a*)=redness value.TA B L E 4Note:

Table 8 .
The result showed that the blending ratio of the four ingredient powders Correlation coefficients between ash content, Fe, Zn, K, Ca, and Mg contents of the formulations of Awaze paste.The effect of blending ratio on % free radical scavenging activity of spicy red pepper paste (Awaze) obtained using actual components as shown by contour plot.Effect of red pepper, garlic, red onion, and ginger on mineral contents (mg/100 g) of formulated spicy red pepper paste (Awaze) and control sample (household made).Note: Data are means ± standard deviation of the replicates.Values with different lowercase letters in the same column are significantly different (LSD, p < .05).Effect of red pepper, garlic, red onion, and ginger on texture profile of formulated spicy red pepper paste (Awaze) and control sample (household made).Data are means ± standard deviation of the replicates.Values with different lowercase letters in the same column are significantly different (LSD, p < .05).
(red pepper, garlic, red onion, and ginger) had a significant (p < .05)TABLE 6 F I G U R E 5TA B L E 8Note: