Garden cress seeds: a review on nutritional composition, therapeutic potential, and industrial utilization

Abstract The growing preference for natural remedies has resulted in increased use of medicinal plants. One of the most significant and varied plants is garden cress (Lepidium sativum), which has large concentrations of proteins, fatty acids, minerals, and vitamins. It also contains a wide range of bioactive components, including kaempferol glucuronide, gallic acid, protocatechuic acid, coumaric acid, caffeic acid, terpenes, glucosinolates, and many more. These substances, which include antioxidant, thermogenic, depurative, ophthalmic, antiscorbutic, antianemic, diuretic, tonic, laxative, galactogogue, aphrodisiac, rubefacient, and emmengogue qualities, add to the medicinal and functional potential of garden cress. An extensive summary of the phytochemical profile and biological activity of garden cress seeds is the main goal of this review. Research showed that garden cress is one of the world's most underutilized crops, even with its nutritional and functional profile. Consequently, the goal of this review is to highlight the chemical and nutritional makeup of Lepidium sativum while paying particular attention to its bioactive profile, various health claims, therapeutic benefits, and industrial applications.

make the seeds a good source of energy for those who are undernourished.Protein (24.2), fat (23.2), carbohydrate (30.7), fiber (11.9),ash (7.1), and moisture (2.9) were found in garden cress seeds (Zia-Ul-Haq et al., 2011).Seeds with low moisture content are more stable, more nutritious, and have a longer shelf life (Alsadee & Agbashee, 2021;Bathish et al., 2021).Various factors influence its proximate composition, such as plant species, seed variation, agronomic techniques, climatic and geographical conditions, and the stage at which seeds are gathered.Fruit and seed nutritional quality may be evaluated using this factor, and more research is encouraged on components that are more intriguing (Zia-Ul- Haq et al., 2011).

| Vitamin and mineral profile
Garden cress is a rich source of vitamin C, also known as ascorbic acid, a water-soluble vitamin originated from glucose metabolism (Valdés Fjad, 2006).It is a reducing agent required for the protection of body from free radical exposure and the synthesis of collagen fibers through proline and lysine hydroxylation.It is not synthesized in the human body due to the absence of glyconolactone oxidase.
Garden cress is considered as a good source of ascorbic acid, and this property boosts its effectiveness against infection and diseases.Sat et al. (2013) reported that ascorbic acid content in L. sativum leaves ranges from 54 to 74 mg/100 g FW.Another study was also done to probe the ascorbic acid content in the stem, leaves, whole plant, and seeds of L. sativum and found 11.74 mg, 7.4 mg, 12.5 mg, and 9.68 mg, respectively (Malar et al., 2014).Moreover, thiamine (0.59 mg), riboflavin (0.61 mg), and niacin (14.3 mg) are present in appropriate amounts in this seed (Gokavi et al., 2004).
The major minerals present in garden cress are potassium and phosphorus, whereas the manganese content is low.It is also a good source of calcium, magnesium, and iron.Calcium (377 mg/100 g) and magnesium (430 mg/100 g) contents of garden cress aid in the correct contraction of muscle for healthy limb and heart motions.
The fatty acid profile of garden cress seeds is characterized by high levels of linolenic (26-34%), linoleic (7.5-11.8%),and arachidic (2-3.5%), which are also helpful for the brain and memory.Palmitic, stearic, and oleic acids are also present in the seeds.Beta-sitosterol and alpha-tocopherol are found in the unsaponifiable materials.
Garden cress oil contains 1422 parts per million (ppm) gamma and 356 parts per million (ppm) delta, 21 parts per million (ppm) alpha and a little amount of beta tocopherol (Ali, 2013).The fatty acid profile varies with the variation in seed variety, agronomic practices, and climatic and geographical conditions.In a study, found that fatty acid content varied among different cultivars of garden cress.In Khiderculivar, arachidonic acid was higher than linoleic acids, whereas in Haraz and Rajab cultivars, behinic and arachidonic acids were higher.Overall, it can be concluded that the nutrients in garden cress are a goldmine and that they may help to lower the incidence of many non-communicable and nutritionally deficient diseases (Ali, 2013).

| Amino acids
The seed protein of garden cress is of high quality and contains necessary amino acids such as lysine (6.26 g) and phenylalanine (5.65 g).Methionine (0.97 g) is a limiting amino acid in the seeds (Gopalan et al., 1971).Garden cress seeds contain all the essential amino acids, except for tryptophan and the S-containing amino acids methionine and cysteine, which are only present in trace amounts.In addition, garden cress seeds are rich in two primary, non-essential amino acids: glutamic acid and aspartic acid.The oilseed analysis showed that aspartic acid (9.76 ± 0.03%) and glutamic acid (19.33 ± 0.19%) were the most abundant amino acids in garden cress seeds (Abdel-Aty, Bassuiny, et al., 2019;Abdel-Aty, Salama, et al., 2019;Sedeeq et al., 2021).
The total essential amino acid percentage is 47.08%, with 6.26 ± 0.39% lysine and 0.97 ± 0.02% methionine.Tryptophan and cysteine were not determined.Essential amino acid score was 28.53% with methionine as the most limiting amino acid.Lysine helps to maintain proper nitrogen balance.The body uses methionine to derive the brain food, choline.It also aids in digestion, as well as serving as a fat burner.It can interact with other substances to detoxify harmful agents and is essential for the production of cysteine and taurine (Zia-Ul- Haq et al., 2012).

| Phytochemicals
Garden cress is considered as a multifarious functional and medicinal plant as it possesses high levels of various phytochemicals such as alkaloids, terpenes, glucosinolates, and saturated and essential fatty acids in the seeds, leaves, seed oil, and roots.Some other important bioactive compounds in garden cress are gallic acid, protocatechuic acid, coumaric acid, caffeic acid, kaempferol glucuronide, and many others.The methanolic extract of defatted seeds has yielded phenolic components such as sinapic acid and sinapin (Oe & Gmelin, 1952).Flavonoids are the most prevalent and abundant class of plant polyphenols that can be obtained from a diet high in plants (Gupta & Morya, 2022).Subclasses of flavonoids (C6-C3-C6) may be divided into flavones, flavanones, flavanols, isoflavones, flavanols, chalcones, and anthocyanins, and they are usually found conjugated to sugars in the form of O-glycosides or C-glycosides, which are the most prevalent (Gupta & Morya, 2022).
The seeds of Lepidium sativum have recently yielded two novel acylated kaempferol and quercetin chemicals, which have just been discovered.The total phenolic and flavonoid contents and antioxidant activity increased several times during the germination of garden cress seeds (Xiao et al., 2014).
High-performance thin-layer chromatography (HPTLC) was used to identify and quantify sinapic acid in a methanolic extract of Gc seed using the imidazole alkaloids semilepidinoside A and B. The sinapic acid was isolated from the glycosylates in Gc seed using a thin layer of silica gel and then analyzed using HPTLC-photo densitometry.Glucotropaeolin 2-phenyl ethyl glucosinolate, also known as gluconasturiin, was found, according to the researchers.Gc seed extracts and glucosinolates were also tested against the white fly, tabaci, for their insecticide properties.When pests were treated with glucotropaeolin, the death rate rose to 92.5% (Abdel-Aty, Bassuiny, et al., 2019).

| ANTI -NUTRITIONAL COMP OS ITION
Antinutritional compounds are those compounds that interfere with the absorption of nutrients in the body and metabolic processes and reduce the absorption of macro-and micronutrients (Singh et al., 2018).Garden cress seeds have a few anti-nutritional compounds such as phytin phosphorus, oxalates, tannins, protease inhibitors, saponins, phytic acid, lectins, and amylase inhibitors.Among these compounds, phytin phosphorus and oxalates are mainly present in raw garden cress seeds (Azene et al., 2022).

| B IOLOG IC AL AC TIVITIE S
Garden cress is a rich source of bioactive compounds, including gallic acid, protocatechuic acid, coumaric acid, caffeic acid, kaempferol glucuronide, and many others, which have been shown to have various health benefits, as mentioned in Table 1.These compounds exhibit anti-inflammatory, anti-carcinogenic, antihypertensive, laxative, and antioxidant properties (Morya et al., 2022).Additionally, garden cress seeds have a bitter taste and possess a range of therapeutic properties, including being thermogenic (promoting heat production), depurative (capable of purifying the body), ophthalmic (effective against eye diseases), antiscorbutic (prevent or cure scurvy), antianemic, diuretic (helps rid the body of salt and water), tonic (restoring and strengthening the nervous system), laxative (to treat constipation), galactagogue (induce, maintain, and increase milk production), aphrodisiac (arouses the sexual instinct, induces venereal desire, and increases pleasure and performance), rubefacient (produces redness of the skin), and emmenagogue (stimulates menstrual flow).

| Antioxidant activity
The antioxidant activity of garden cress seeds is mainly due to the presence of phenolic compounds, phytosterols, and tocols, with tocopherols being the major contributor.Tocopherols act as scavengers of free radicals and inhibit oil oxidation, thus preventing various diseases.This property makes garden cress seeds highly valuable for the production of various dietary and functional products on an industrial scale.Moreover, garden cress seeds exhibit high DPPH antiradical activity, making them ideal for fortification in the development of various designer foods (Al-Saad & Al-Saadi, 2021).
Researchers looked into the antioxidant properties of the ethanolic extract of Lepidium sativum leaves, stem, whole plant, and seeds by different methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), total glutathione assay, reduced glutathione activity, and reducing power assay.The whole plant exhibited the greatest scavenging activity (12.1902%), while the stem exhibited the least amount (2.6905) (Malar et al., 2014).
Additionally, coumaric acid, another phenolic compound present in garden cress seeds, has been shown to possess antioxidant and anti-inflammatory properties.Glutathione S-transferase activity was highest in the seed (9600-56.3g/mL) than in any other plant tissue studied.The ethanolic extracts of Lepidium sativum leaf had a lower glutathione concentration (9-0.2 g/mL).Alcoholic extracts were the most effective in the reducing power test (Kadam et al., 2018).
In a study, Kadam et al. (2018) explored the antioxidant activity of Garden cress ethanolic seed extract through different assays and found 187.12, 162.4, 119.32, and 35.29

| Antidiabetic activity
The optimal dosage of 20% seed methanol extract of L. sativum to regulate blood glucose and treat hyperglycemia was 300 mg/kg BW.
The methanol extract of L. sativum was able to regulate diabetes, increase antioxidants, and improve the lipid profile of the subjects (Kadam et al., 2018).In both NIDDM and healthy volunteers, the anti-diabetic effects of Lepidium sativum seed powders were studied.Hypoglycemic activity was seen in a 21-day therapy regimen.
More than 300 million people will have diabetes or chronic hy- and other Arabic nations are known for their usage of this plant in the treatment of a variety of ailments.As a phytotherapy for hypertension, diabetic management, and renal illness or as a salad additive, L. sativum is often suggested (Eddouks et al., 2002).

| Anti-inflammatory effect
Sativum seeds contain 24 percent oil that is mostly composed of the omega-3 and omega-6 fatty acids, ALA and LA, respectively (12%).
The antioxidants and phytosterols in this oil make it resistant to oxidation.The Wistar rats' spleens and lungs showed synergistic effects of L. sativum oil (LSO) suppression of platelet aggregation and thromboxane B2 levels.Other studies have shown that LSO reduces lymphocyte proliferation and inflammatory mediator generation from peritoneal macrophages in rats (Diwakar et al., 2010).
Garden cress stems, leaves, and seeds are all good for your health (Jabeen et al., 2017).Tocopherol levels and antioxidant enzyme activity were raised in Wistar rats fed a diet enriched with LSO for 60 days (Jabeen et al., 2017).Phytochemicals present in garden cress seeds have shown antioxidant and anti-inflammatory activity in various studies.

| For the treatment of iron-deficiency anemia
Research has shown that vitamic C supplementation helps improve iron absorption.It also boosts the digestive system's ability to absorb iron.Vitamin-C and iron-rich seeds of garden cress may be used to treat anemia or iron insufficiency without the need for additional supplements (Umesha & Naidu, 2015).In the stomach, L-ascorbic acid helps to increase iron absorption by building a chelate with ferric iron at an acidic pH.This makes the iron more soluble at the alkaline pH of the duodenum, where it is more easily absorbed.Improves hemoglobin levels and cures anemia by consuming Garden Cress seeds on a long-term basis.Garden cress seeds have a high concentration of iron, which encourages the development of red blood cells (Falana et al., 2014).
During adolescence, a person's physical and mental development accelerates.Adolescents with low iron levels may not be able to reach their full development potential because of a lack of iron in their diet.The seeds of garden cress are said to have galactogenic and antioxidant characteristics, as well as a great deal of promise as a functional food (Kumar et al., 2017), as mentioned in Figure 1.

| Management of menstrual cycle disorders
Maintaining a regular menstrual cycle and having knowledge of the estimated date of conception are crucial for women throughout their lives.It is believed that the ingestion of garden cress seeds may assist in regulating menstrual cycles since the seeds contain estrogen-like chemicals (Diwakar et al., 2010).On the subject of garden cress seed and reproduction, there is a wide range of opinions.It is also missing information on effectiveness, safety and how it works.It is also unknown how it affects LH secretion and reproductive function within the mammalian species studied.Rats (1.6 mg/g BW) given garden cress seed powder for 14 days showed a mammogenic and lactogenic effect.Aside from that, the ovariectomized rats administered a methanolic extract of LS for 21 days showed proceptive and receptive properties when 200 and 400 mg/kg BW were provided orally.
A similar effect on prolactin, progesterone, and luteinizing hormone in ovariectomized rats has been observed (Patel et al., 2009).

| For breast milk secretion
Nutritional significance for breastfeeding mothers is well known with the seeds of garden cress.It aids the nursing mother in sustaining the flow and production of breast milk that is rich in protein and iron (Imade, Erinfolami, et al., 2018;Imade, Smith, & Gazal, 2018).
Garden cress seeds have several properties, including being a tonic, demulcent, rubefacient, carminative, galactagogue, and emmenagogue.It has been found to help an average of 11 nursing mothers produce more milk throughout the postnatal period.Additionally, it is often recommended for those suffering from diarrhea or dysentery (Prajapati & Dave, 2018).Syphilis and Tenesmus may be treated with the root, which is bitter, caustic, and used as a condiment.Male aphrodisiac effects and an increase in testosterone concentration have been linked to garden cress, whereas female anovulatory and abortifacient effects have been linked to garden cress (Uphof, 1959).
Breast milk is an excellent source of nutrition and immune system support for infants.Graden cress seeds are excellent galactagogues, and their consumption is highly recommended for nursing mothers (Khan, 2018).

| Anticancer activity
Recent research suggests that garden cress may be used to combat cancer.This product includes antioxidants, including Vitamin A and Vitamin E, which protect cells from free radical damage.Additionally, it contains a bioactive compound that has been shown to inhibit the synthesis of enzymes that can lead to tumor growth.Studies have demonstrated that the injection of this compound is effective in killing breast cancer cells (Imade, Smith, & Gazal, 2018).Antioxidant, anti-inflammatory, and cancer-protective properties have been found in L. sativum's phytoesterols as well as their metabolites (Singh et al., 2015).Phenolic chemicals, particularly flavonoids, may protect the human body against oxidative stress, which may contribute to cancer, aging, and cardiovascular illnesses.For example, Table 1 indicates that gallic acid has been found to inhibit apoptosis and cancer-causing pathways in the body (Conforti et al., 2008;Hazafa et al., 2022;Singh et al., 2015) (Figure 2).

| Healing properties of Lepidium sativum for bone fractures
There are several health benefits associated with the seeds of Lepidium sativum, including the healing of fractures, and variables that might delay or hinder this healing process.The plant's anatomical parts, especially the seeds, exert their effects.Some research was performed on animal models to assess the healing properties of graden cress, and it was noticed that garden cress increased collagen deposition and tensile strength at the fracture sites and radiologically accelerated callus development in rabbits (Ahmad et al., 2021;Bin Abdullah Juma & Martin, 2011).
The mucilage extracted from the dried seed coat of garden cress seeds has been used as an excipient in various pharmaceutical formulations (Dixit Jr et al., 2020).The use of L. sativum caused a noticeable increase in collagen deposition at the location of the fracture, which was connected to a considerable improvement in the fractured tibia's tensile strength.This investigation supports the plant's traditional use as a bone-healing agent (Prajapati et al., 2014).
In another study, the test group's diet included Lepidium sativum seeds, and x-rays were utilized to track the healing status of the fracture over the course of 6-12 weeks.Anecdotal evidence and statistical evidence suggest that the traditional usage of Lepidium F I G U R E 1 Mechanism of garden cress seeds in the treatment of iron-deficiency anemia.
sativum for speeding up bone fracture healing is justified.Studies like this demonstrate the necessity for more investigation into this topic (Ahsan et al., 1989).
In Saudi Arabia and other Arabic nations, plants and seeds are widely accepted as useful mediators for the healing of damaged bones.Research was done on six adult New Zealand white rabbits to demonstrate this effect.Everyone had fractures in the middle of their left femur, which were classified into two categories.During the experiment, one group of rabbits had regular food mixed with garden cress seed, whereas the other group received simply a normal diet (Ahmad et al., 2021).X-rays of the induced fractures were collected at 6 and 12 weeks postoperatively to monitor the healing process.Test group fracture recovery was statistically superior to the control group, according to the findings (Ahmad et al., 2015).

| Osteoarthritis intervention activity
Research was conducted to investigate the efficacy of Lepidium sativum seeds in treating osteoarthritis.The study involved two groups of patients.A control group received starch as a placebo, while the drug group received Lepidium sativum powder split into two daily doses of 6 gm each for a period of 30 days.Evaluations of osteoarthritis symptoms were conducted before and after treatment to determine whether there were any improvements.Results showed that more than three-quarters of patients in the drug group experienced significant improvement in symptoms, with 30% achieving full remission, 37.5% showing substantial progress, and 25.5% reporting moderate progress (Ahmad et al., 2015;Al-Snafi, 2019).The seeds have been shown to accelerate glucocorticoid-induced osteoporosis in rats.Conversely, they have been found to promote faster healing of artificial fractures in the midshaft of the left femur in rabbits.
However, the anti-osteoporotic effects of the seeds and the underlying mechanisms have not been studied in ovariectomized rats (bin Abdullah Juma, 2007; Rahman et al., 2004).
It has been suggested that Lepidium sativum can be a useful addition to current osteoarthritis treatment regimens due to its analgesic and anti-inflammatory properties, as well as its high concentration of Ca 2+ ions.Only a small number of patients in the control group showed improvement, which can mostly be attributed to the eradication of causal factors that was provided to all patients in both groups (Rahman et al., 2004).

| Anti-hypercholesterolemic effect
A study demonstrated that feeding rats with a diet supplemented with garden cress seed oil led to significant reductions in triglycerides, total cholesterol, LDL-c, VLDL-c, and AST and ALT enzyme levels compared to the positive control group.Interestingly, HDL levels Mechanism of gallic acid's anticancer activity.
were found to be slightly elevated in rats given varying doses of garden cress oil.These findings suggest that a diet rich in garden cress oil could have beneficial effects on the blood lipid profile and plasma lipoproteins in individuals with dyslipidemia (Kraft, 2015).Garden cress seeds have not yet been studied for their cholesterol-raising properties.Unripe carnauba fruit aqueous pulp extracts were further studied for their hypocholesterolemic efficacy in experimental animals fed a high-cholesterol diet (Youssef et al., 2014).

| Effect on bronchial asthma/digestive functions
Sore throats, asthma, headaches, and coughing may be alleviated with the use of garden cress seeds.Due to the fact that it has the properties of a bronchodilator, it is recommended for patients with bronchitis (Imade, Erinfolami, et al., 2018;Khan, 2018).A study was conducted on 30 male and female patients aged 15-80 with mild to moderate bronchial asthma, excluding pregnant women.The patients were administered 1 g of finely powdered seed powder orally, three times a day, for a period of 4 weeks.The spirometer results indicated significant improvements in several pulmonary functions during and after the study period, with no reported adverse effects among the subjects (Doke & Guha, 2014).
Table 2 indicates that the ingestion of a large quantity of goiter can lead to hypothyroidism.However, Lepidium sativum L.
has demonstrated its effectiveness in treating bronchial asthma, hiccups, coughs with expectoration, and bleeding piles.Several Ayurvedic practitioners have recommended the use of L. sativum seeds (Rehman et al., 2012).There are a variety of medical uses for the plant.Patients with bronchial asthma benefit from the diuretic and mildly stimulating properties of the leaves of this plant.L. sativum, on the other hand, has not been studied in terms of its ability to cure bronchial asthma (Chopra et al., 1986).

| Antihypertensive effect
The objective of this section was to study the antihypertensive properties of Lepidium sativum L. on spontaneously hypertensive rats (SHR).Lepidium sativum L., a plant belonging to the Lepidium sativum family, has been found to have hypotensive and diuretic effects on both normotensive (WKY) and spontaneously hypertensive rats (SHR) (Paranjape & Mehta, 2006).Aqueous LS extract (20 mg/kg for 3 weeks) significantly reduced blood pressure in SHR rats, while no significant reduction was seen in WKY rats.Treatment with SHR rats considerably reduced the systolic blood pressure between day 7 and day 30.However, in WKY rats, LS extract considerably increased water excretion (p = .001),while in SHR rats, there was no statistically significant difference (Wadhwa et al., 2012).
As a result, WKY rats were given 20 mg/kg aqueous LS extract orally, and the excretion of sodium, potassium, and chlorides in the urine was significantly increased.The aqueous LS extract treatment increased sodium, potassium, and chloride urine elimination in spontaneously hypertensive rats (Kagathara et al., 2009).Table 2 indicates that normal rats exhibited a substantial increase in glomerular filtration rate following oral administration of LS, but SHR rats showed no significant change throughout the treatment period.
Furthermore, there were no significant changes in the heart rate of SHR and WKY rats following LS therapy (Maghrani et al., 2005;Verma et al., 2021).

| Food industry
Over a period of 6 months, a group of researchers investigated the effects of a health mix that included garden cress seeds on a sample of 100 teenage females who were suffering from anemia in the Dindigul area of Tamil Nadu (Besufekad et al., 2018).The health mix, which contained 5 g of garden cress seeds, 20 g of rice flakes, 5 g of bajra, 5 g of roasted Bengal gram dhal, and 5 g of samai, provided 10 mg of iron in a 50-g serving.The researchers monitored the participants' hemoglobin levels before and after the six-month supplement period.The results showed a significant increase in mean hemoglobin levels from 8.23 ± 0.49 to 11.11 ± 0.46 after the followup period (Azimkhanova et al., 2021).
A second study investigated the impact of garden cress ladoo on the hemoglobin levels of anemic 12-to 15-year-old females.The study included 100 moderately undernourished girls, who were divided into two groups: an experimental group and a control group.The control group did not receive any deworming or supplementation.After 6 months, the experimental group had a significant increase of 3.8 g/ dL in hemoglobin levels, while the control group showed a slight rise of only 0.1 g/dL.The experimental group also reported a decrease in anemic symptoms such as exhaustion and overall weakness, whereas the control group showed no significant changes (Abuelgasim et al., 2015).
When it comes to cakes and breads of all kinds (including a variety of multigrain varieties), they've recently found their way into salad dressings and soup mixes as well as in ready-to-eat morning cereals and beverages like cereal bars (Abo El-Maati et al., 2016) (Figure 3).Some food items and bakery goods use flaxseed as a nutritious component, and its flour is used in the production of bread.
Ethiopians eat the seeds, which are boiled, crushed, mixed with seasonings and water, served with Injera and breads, used to prepare food and edible oil, and also consumed as a porridge and beverage called chillika (Angel & Devi, 2014).

| Dahiwala bread
Dahiwala bread, a simple breakfast meal, is made by combining bread slices with curd and baking them.To reduce the amount of saturated fat in the bread, the mixture is cooked in soybean oil.Additionally, garden cress seeds are added to boost the nutritional value of the bread, TA B L E 2 Systematic pharmaceutical case studies and key findings of garden cress.Along with this, molasses and coconut kernel garden cress seed powder have numerous health benefits

Can reduce anemia
Improved shelf-life and consumer acceptance of fresh-cut and fried potato strips by an edible coating of garden cress seed mucilage A mucilage coating made from garden cress seed is added to the potato after cutting and studied for shelf life and oil intake while frying It is observed that the browning reaction and the shelf life of the potato are improved after the addition of the garden cress seed mucilage and also show little intake of oil after frying increasing its protein, fat, calcium, iron, and phosphorus content.Due to its medicinal characteristics, this product can be both nutritious and beneficial for treatment purposes (Angel & Devi, 2015).
A recent study has shown that the addition of Garden Cress Seeds (GCS) can improve the rheological qualities and overall quality of rice-wheat bread (Bhardwaj et al., 2020).Four different amounts of G and L hydrocolloids (0%, 0.3%, 0.6%, and 1%) were added to the flour to create composite bread.The study found that GCS served as a novel and effective gluten substitute, enhancing the quality and texture of the bread.These properties could be particularly beneficial for functional food preparations aimed at addressing issues such as milk production and anemia in women (Eyres & Eyres, 2014).

| Health drinks
Garden cress seeds are a rich source of protein, carbohydrates, and critical minerals such as calcium, iron, and phosphorus, as well as crude dietary fiber (7.6%).These seeds are commonly used to make health beverages with milk, such as milk-based health drinks.Typically, these drinks contain 5% sugar (w/v), 1% fat, and 3% processed garden cress seeds (Agarwal & Sharma, 2013).This product is beneficial for In a recent study, researchers examined the effects of Garden Cress seed oil on lipid metabolism by combining it with vegetable oil.
Garden cress oil is high in α-linolenic acid, an essential omega-3 fatty acid.Traditionally, vegetable oils such as sunflower, maize, safflower, and soybean oil have been recommended to have a 10:1 or 2:1 ratio of n-6 to n-3 PUFA.However, the study found that blending Garden Cress seed oil with other vegetable oils could produce a PUFA ratio of 2.3-2.6.Rice bran oil, sunflower oil, and sesame oil were used in the experiments (Zanvar & Rohini, 2007).

| Uses of animal feed additives in broilers
Additives for animal feed are utilized all over the globe for a variety of reasons.To ensure that animals get the necessary nutrients they need, certain feed additives are included in their diets.Other feed additives

Title of the study Study information Key findings
Reproductive performance and milk yield of rabbits fed diets supplemented with garden cress (Lepidium sativum) seed The reproductive and milk-yielding capacities of rabbits are studied while giving the garden cress seed as a supplement It showed that the garden cress seed has a positive impact on the reproductive as well as milk-yielding of rabbits Garden Cress (Lepidium sativum) seeds ameliorated aluminum-induced Alzheimer's disease in rats through antioxidant, anti-inflammatory, and antiapoptotic effects The study examined the impact of the administration of garden cress seed extract on biochemical, cerebral, histopathological, and behavioral, and changes in rats with AlCl 3 -induced AD and explored the mechanism behind this effect It showed changes due to its antioxidant, antiapoptotic, and anti-inflammatory effects, suggesting that it has a neuroprotective effect TA B L E 2 (Continued) F I G U R E 3 Food products of garden cress seed.
are added to increase animal performance by increasing the amount of food they consume and their ability to develop (Salehi, 2019;Schmitz & Ecker, 2008).Anti-microbial drugs in animal feed are not permitted in many countries throughout the globe because of the current trend in animal nutrition toward more "natural" diets (Umesha & Naidu, 2012).
This has sparked a renewed interest in the feed sector to explore alternatives that may be acceptable to the public.Recent studies have shown that probiotics, prebiotics, herbs, spices or botanicals (e.g., essential oils), enzymes, and minerals are all excellent alternatives.The use of herbs, spices, and their extracts can have a significant impact on the health of chickens in multiple ways.All of these properties contribute to the development of immunity and growth by providing antioxidant and antibacterial properties.Regulating feeding and stimulating digestive secretions may improve digesting capacity and lower the risk of digestive problems (Zeilab Sendijani et al., 2020).
Herbs have been used for medicinal purposes since ancient times due to their availability, low side effects, and effectiveness in treating various ailments in both humans and animals.However, studies on the use of herbal combinations in broiler diets have yielded mixed results.While some researchers have reported improved broiler performance, others have found no significant impact on body weight gain, feed intake, or feed conversion.In Ethiopia, Lepidium sativum has been traditionally used by farmers for both medicinal and dietary purposes, highlighting its potential as a valuable ingredient in animal feed (Strasser et al., 2000).
The leaves, roots, and seeds of garden cress have a long history of traditional use in many countries as a bone fracture healing agent, anti-asthmatic, antiscorbutic, aperient, diuretic, galactogogue, poultice, stimulant, and blood pressure regulator.Garden cress seeds are also known to have a positive impact on weight gain due to their high concentration of n-3 polyunsaturated fatty acids, tocopherols, lignans, and antioxidants (Taghipour et al., 2021).

| Dietary supplements
Studies have found that the cress seed had a high (86.90%)water absorption capacity (229 mL H 2 O/100 g).Garden cress seed can be used as an ingredient in the meat, bread, and cake industries.
Aside from being a nutritious supplement or nutrient substitute, the protein isolated from the seed of garden cress can be used in food systems (Shawle et al., 2016).Cress seed is rich in iron, protein, and calcium, and it may be used in the treatment of various health problems.A wheat-based food product like Mathri can be prepared by using cress seed powder at different levels (2.5%, 5%, and 7.5%).
Particularly, 5% husk-removal GCSP was found to have a higher nutritional value than standard mathri, as well as the ability to protect against oxidative stress (Zia-Ul- Haq et al., 2012).

| Role in the processed spread cheese
Cheese-based foods, such as sandwiches, hamburgers, pizza, and lasagna, often use processed cheese products as an ingredient.
Sadly, certain emulsifiers used in the manufacturing of cheese have been linked to health issues (Hacıseferoğulları et al., 2005).Sodium phosphate, a common component in processed cheese products, has been shown to cause kidney damage.Additionally, tartrate may produce diarrhea, and potassium phosphate might lead to an allergic response.As a result, there is still a pressing need to find novel emulsifying agents (Ghamari et al., 2021).
In a study, cress seeds were used to make processed spread cheese rather than salt as an emulsifying agent.When making processed spread cheese, cress seed powder was made and applied in six different ratios (0.05%, 1.5%, 2.5%, 3.5%, 4.5%, and 5.5%) in contrast to the control (3% commercial emulsifying salt).Results showed that 3.5% cress seeds showed significant health-enhancing qualities, particularly in decreasing glucose ratios (Punia & Dhull, 2019).
Lepidium sativum is a good source of protein and dietary fiber, as well as vital amino acids.Cress seeds have been shown to be safe and non-toxic by toxicology investigations.Cress seed has been shown to be beneficial in the treatment of hypertension, renal illness, and diabetes.Hypocholesterolemic and antidiarrheal qualities are among its numerous therapeutic benefits.Fracture healing, diuretic, and nephroprotective properties are also present.Galactogogue and anti-inflammatory and antipyretic properties are also present (Mohamed & Hussein, 2017).

| Natural super-disintegrant
Disintegrants are commonly used in tablet formulations to facilitate faster dissolution by breaking them down into smaller particles.
Various studies have been conducted to compare the effectiveness of natural and synthetic superdisintegrants.In one such study, mucilage obtained from lentil seeds (L.sativum) was used to develop fastdissolving tablets of Nimesulide, a nonsteroidal anti-inflammatory drug (NSAID) (Ramadan & Oraby, 2020).The study revealed that using a 10% mucilage concentration resulted in a greater dissolution of the tablet.Additionally, mucilage derived from lentil seeds was used to create oral disintegrating tablets of Metformin HCl through the direct compression process (Saju & Sivaraman, 2021).
Garden cress seeds contains mucilage, which functions primarily based on swelling mechanisms that act as binders and disintegrating agents.Compared to leaves, seeds often have a higher mucilage concentration (Iswariya et al., 2021).In fact, research comparing natural and synthetic superdisintegrants found that L. sativum mucilage performed better overall than the synthetic alternative (Dixit Jr et al., 2020).

| Controlled-release system application
Solid controlled-release oral unit dose pharmaceutical compositions have been developed for oral administration using hull powder from L.
sativum seeds.The compositions contain one or more medicines and the gel-forming husk powder from L. sativum seeds in concentrations ranging from 10% to 70% of the total weight of the dose form.A novel interpenetrating polymer network (IPN) composed of L. sativum and PVA was constructed for the first time by the researchers, who then employed the IPN as a cross-linker to generate microspheres containing simvastatin as the model medication by cross-linking the microspheres utilizing emulsion cross-linking (Rani & Ahuja, 2017).

| OTHER APPLI C ATI ON S
Garden cress gum and maltodextrin were used as microencapsulation coats for the entrapment of garden cress phenolic-rich extract for improving its storage stability and antioxidant and antibacterial activities (Abdel-Aty et al., 2023).Cress seed mucilage, either alone or in combination with a synthetic polymer, may be utilized to provide a novel drug delivery mechanism for patients (Moreira et al., 2019;Nerkar & Gattani, 2012).Cress seed gum improved the dough's extensibility value from 0.3% to 0.6%, followed by a decrease at 1%.
As cress seed gum concentrations rise, bread's water activity and crumb firmness both deteriorate.This has led to the development of novel edible films and coatings based on cress seed gum.In addition to its inexpensive cost of manufacture and hydrophilic, biocompatible, and biodegradable characteristics, it also has strong rheological qualities (Razmkhah et al., 2016).
Since the Vedic period, it has been regarded as a major source of nourishment and medicine because of its health-enhancing characteristics.Garden cress aids in the removal of toxins from the body and increases food intake.Functional meals may be made from the entire edible seed (Uphof, 1959).

| CON CLUS IONS
The present review thoroughly examined the nutritional makeup, potential for therapeutic use, and industrial application of garden cress seeds.The nutritional profile highlights the seeds' abundance of important nutrients, antioxidants, and bioactive substances, establishing them as a desirable dietary supplement.Garden cress seeds have proven to have anti-inflammatory, antioxidant, anti-cancer, and antibacterial qualities, according to research on their therapeutic potential.
Literature has demonstrated that garden cress seeds have a positively significant effect on the growth of children and adolescent girls.The seeds' potential as functional food ingredients and nutraceuticals is suggested by their ability to positively impact health disorders like diabetes and cardiovascular illnesses.The analysis also examined garden cress seeds' industrial uses, highlighting their adaptability in the food, pharmaceutical, cosmetic, and agricultural industries, further emphasizing their potential role in sustainable industrial practices.It is clear from this review that garden cress seeds are an important but littleknown resource with a variety of uses.The synopsis of knowledge provided brings together previously available data but also identifies directions for future investigation, promoting more research into the complex properties of garden cress seeds, such as their potential synergies in different industrial processes and their particular therapeutic uses in health and wellness.Garden cress seeds are a viable option for industrial and nutritional uses in a world where there is an increasing need for sustainable and health-promoting resources.This review sets the stage for continued research, innovation, and practical applications, inviting scientists, industries, and health professionals to collaborate in unlocking the full potential of garden cress seeds for the benefit of both human health and sustainable industrial practices.
by the year 2025.Free radicals are excessively produced because of the long-term hyperglycemia.Compared to current synthetic medications, plant-based herbal therapy is thought to be less harmful and devoid of side effects.If you've ever wondered what the name "habarachad" means, you're not alone.Saudi Arabia, Sudan, Alternative and natural breast cancer treatments are being investigated to minimize or eliminate the drawbacks of traditional therapies.Many chemo-preventive and therapeutic components have been identified in the seeds of L. sativum L. (garden cress)(Hazafa et al., 2020).Flavonoids found in L. sativum seeds and leaves were tested for their cytotoxic effects on Hep.G2 cells.L. sativum seed Oglycoside extract (ethyl acetate extract) exhibited the highest cytotoxic impact on Hep.G2 cells, with butanol seed extract in third place.Kaemferol present in the garden cress seeds has been found to inhibit many forms of cancer, such as lung cancer, brest cancer, brain tumors, prostate cancer, and hepatic cancer(Mahassni & Al-Reemi, 2013).

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wide range of individuals, including children, the elderly, newborns, and convalescent patients.The milk-based beverage enriched with Garden Cress seeds contains all the vital nutrients required for these individuals, such as protein, carbohydrates, calcium, iron, phosphorus, and crude dietary fiber.Consuming this supplement can help prevent nutritional deficiencies and promote lean muscle growth, especially in those who regularly engage in physical activity.