Peganum harmala L. extract‐based Gold (Au) and Silver (Ag) nanoparticles (NPs): Green synthesis, characterization, and assessment of antibacterial and antifungal properties

Abstract During the last decade, nanotechnology has attained a significant place among the scientific community for the biosynthesis of plant‐based nanoparticles owing to its effective, safe, and eco‐friendly nature. Hence, keeping in view the significance of nanotechnology, the current study was conducted to develop, characterize (UV–visible spectroscopy, scanning electron microscopy, Fourier‐transform infrared spectroscopy, and energy‐dispersive X‐ray spectroscopy), and assess the antimicrobial (antibacterial and antifungal) properties of Peganum harmala L. Extract‐based Gold (Au) and Silver (Ag) nanoparticles (NPs). Characteristic absorption peaks at 420 and 540 nm revealed the formation of AgNPs and AuNPs, respectively. SEM images revealed that both silver and gold nanoparticles were oval and spherical with average size ranging from 42 to 72 and 12.6 to 35.7 nm, respectively. Similarly, FT‐IR spectra revealed that the functional groups such as hydroxyl, carboxyl, and polyphenolic groups of biomolecules present in the extract are possibly responsible for reducing metallic ions and the formation of nanoparticles. Likewise, the EDX analysis confirmed the presence of silver and gold in synthesized NPs. Furthermore, the AgNPs and AuNPs showed good antibacterial and antifungal activities. The maximum antibacterial and antifungal activity was noticed for P. harmala extract against Pseudomonas aeroginosa (21 mm) and Candida albicon (18 mm), respectively. Whereas, the maximum antibacterial and antifungal activities of synthesized AgNPs were observed against Salmonella typhi (25 mm) and Penicillium notatum (36 mm), respectively. Moreover, in the case of AuNPs, the highest antibacterial and antifungal activity of synthesized AuNPs was noticed against Escherichia coli (25 mm) and C. albicon (31 mm), respectively. Findings of this study revealed that P. harmala extract and biosynthesized NPs (silver and gold) possessed significant antibacterial and antifungal properties against different bacterial (Bacillus subtilis, Staphylococcus aureus, E. coli, P. aeroginosa, and S. typhi) and fungal (C. albicans, Aspergillus Niger, and P. notatum) strains. Further studies must be carried out to assess the probable mechanism of action associated with these antimicrobial properties.

tibacterial and antifungal) properties of Peganum harmala L. Extract-based Gold (Au) and Silver (Ag) nanoparticles (NPs).Characteristic absorption peaks at 420 and 540 nm revealed the formation of AgNPs and AuNPs, respectively.SEM images revealed that both silver and gold nanoparticles were oval and spherical with average size ranging from 42 to 72 and 12.6 to 35.7 nm, respectively.Similarly, FT-IR spectra revealed that the functional groups such as hydroxyl, carboxyl, and polyphenolic groups of biomolecules present in the extract are possibly responsible for reducing metallic ions and the formation of nanoparticles.Likewise, the EDX analysis confirmed the presence of silver and gold in synthesized NPs.Furthermore, the AgNPs and AuNPs showed good antibacterial and antifungal activities.The maximum antibacterial and antifungal activity was noticed for P. harmala extract against Pseudomonas aeroginosa (21 mm) and Candida albicon (18 mm), respectively.Whereas, the maximum antibacterial and antifungal activities of synthesized AgNPs were observed against Salmonella typhi (25 mm) and Penicillium notatum (36 mm), respectively.Moreover, in the case of AuNPs, the highest antibacterial and antifungal activity of synthesized AuNPs was noticed against Escherichia coli (25 mm) and C. albicon (31 mm), respectively.Findings of this study revealed that P. harmala extract and biosynthesized NPs (silver and gold) possessed significant antibacterial and antifungal properties against different bacterial (Bacillus subtilis, Staphylococcus aureus, E. coli, P. aeroginosa, and S. typhi) and fungal (C.albicans, Aspergillus Niger, and P. notatum) strains.Further studies must be carried

| INTRODUC TI ON
During the last two decades, nanotechnology has played a vital role in the scientific community for the development of novel drugs.Literature reveals that advanced attributes of the materials can be achieved when operating at nano-scale owing to the associated quantum size of materials.In modern scientific era, nanotechnology is playing a significant part in diverse fields including biological sciences, physical sciences, and engineering (Ahmad, Ali, et al., 2010;Ahmad, Waheed, et al., 2010).Nanotechnology has signified opportunities for advancements in areas like synthetic materials and biological systems (Ansari et al., 2012).Recently, nanotechnology has been employed for the development of biosensors and biomedicines to overcome the challenges faced during drug delivery and management of different abnormalities.
Moreover, nanotechnology has also imparted significant impact in the field of photo devices, physics, electronics, and energy materials (Asharani et al., 2008).In recent times, various medicinal plants have been used for the synthesis of nanoparticles owing to the emergence of green chemistry.For this purpose, extracts of different parts of several plants have been used for the biosynthesis of metallic nanoparticles (Alomar et al., 2020).Diverse noble metals such as silver, gold, palladium, and platinum have extensively been used for the biosynthesis of plant-based nanoparticles (Zhang et al., 2016).
Generally metallic nanoparticles offer unique properties that make them attractive for various applications, their potential toxicity issues are significant considerations.Therefore, the need to synthesize safe and co-friendly metallic nanoparticles is considered a top priority of the scientific community.Purposely, the biosynthesis of silver nanoparticles has attained a hallmark position for scientists owing to their safe and environment-friendly synthesis (Murugan & Shanmugasundaram, 2014;Zangeneh et al., 2019).In medical science, metallic nanoparticles are used for their catalytic degradation, anticancer, antibacterial, antiviral, and anticancer properties.
Researchers are focusing on the development of metallic nanoparticles due to their reduced particle size, enhanced surface area, and improved chemical and physical properties.Silver and gold nanoparticles are being used industrially for the production of biocatalysts and biomedicines.Moreover, they are employed in food packaging and as antimicrobial agents in medical devices (Alhumaydhi et al., 2021;Bawazeer et al., 2022).
Since ancient times, various plants have been used by folk people for the treatment of different ailments like constipation, inflammation, diabetes, and microbial infections (Alomar et al., 2020;Hagh-Nazari et al., 2017;Zangeneh et al., 2018).Plant-based silver (AgNPs) and gold (AuNPs) nanoparticles are among the easiest and most frequently prepared in research and development (Hano & Abbasi, 2021;Singh et al., 2021).Plant extracts and isolates are considered to be viable alternatives to synthetic drugs owing to their safe nature (Goorani et al., 2019;Zangeneh et al., 2018).
Various plant extracts such as Ulva flexuosa, Withania somniferous, Calendula officinalis, Melia azedarach, Euphorbia antiquorum, and Silybum marianum have been used for the synthesis of metallic nanoparticles (Prabhu & Poulose, 2012).In comparison to conventional procedure, green biosynthesis is considered to be eco-friendly, cost-efficient, rapid, and safe for the formulation of nanoparticles (Jha et al., 2009).Plant extracts are characterized as safe, eco-friendly, and economical alternatives to other biological sources such as fungi, algae, and bacteria (Ahmad, Ali, et al., 2010;Ahmad, Waheed, et al., 2010).
Peganum harmala, a glabrous plant, is frequently cultivated throughout the eastern Mediterranean region.P. harmala is extensively distributed in semi-soiled areas and belongs to the family Zygophyllaceae.In various central Asian and North African regions, P. harmala has been prescribed by indigenous practitioners as a valuable medicinal plant since ancient times (Hemmati et al., 2020).This plant has been administrated for the treatment of hypertension and other heart-related diseases.Various studies have shown the potential of P. harmala extract as an effective agent in lowering hypertension and possessing antigenic properties (Yusof et al., 2010).Accordingly, Nayem et al. (2020) showed that the extract of this plant possesses the potential in managing elevated blood pressure and aid vascular resistivity (Nayem et al., 2020).The utilization of P. harmala extract as a platform for nanoparticle synthesis holds significant promise due to its unique chemical composition and bioactive properties.P. harmala has garnered attention in the field of green synthesis for its ability to facilitate the reduction and stabilization of metallic nanoparticles, offering advantages over plants because of cost effectiveness, easy availability, and versatile phytochemical profile.In this study, we aimed to harness the therapeutic potential of P. harmala extract for the green synthesis of Ag and Au nanoparticles, and subsequently evaluate their antimicrobial activities.The novelty of our study lies in the comprehensive exploration of P. harmala extract-mediated green synthesis of Ag and Au NPs and their subsequent assessment for antimicrobial properties.While previous research has highlighted the potential of various plant extracts for nanoparticle synthesis, our study uniquely focuses on P. harmala, a plant known for its medicinal properties, particularly in cardiovascular health (Nayem et al., 2020).By leveraging the bioactive constituents of P. harmala extract, we successfully synthesized Mohammed Mansour Quradha, College of Education, Seiyun University, Seiyun, Hadhramawt, Yemen.Email: mm.quradh@seiyunu.edu.ye

Funding information
Pakistan Science Foundation out to assess the probable mechanism of action associated with these antimicrobial properties.

K E Y W O R D S
antibacterial, antifungal activity, Peganum harmala; Ag and Au NPs Ag and Au nanoparticles using a green and sustainable approach.
Our study offers a systematic characterization of the synthesized nanoparticles using advanced analytical techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), UV-visible spectroscopy, and energydispersive X-ray spectroscopy (EDX).Furthermore, the evaluation of the antimicrobial activities of the synthesized nanoparticles against a diverse panel of bacterial and fungal strains adds significant value to the existing literature.Overall, our study contributes to the growing body of knowledge on green synthesis methodologies and underscores the potential of P. harmala extract-mediated nanoparticle synthesis for biomedical applications, particularly in antimicrobial therapy.

| Chemicals and reagents
All the chemicals and reagents used in this study were analytical grade.Methanol and Hydrogen tetrachloroaurate trihydrate [HAuCl 4 •3H2O] were purchased from Merck, Germany.While, Sodium chloride, lead chloride, manganese chloride, and potassium chloride were procured from Sigma, Germany.

| Collection, identification, and extraction
In October 2020, a mature P. harmala plant was gathered from Swabi, KPK, Pakistan.The plant was identified by Mr. Muhammad Irfan, a lecturer in the Department of Botany, University of Swabi, KPK, Pakistan.The voucher specimen number UOS/Bot-2111 was deposited in the herbarium of said Department.Leaves (1 kg) were shadedried and turned to powder using pestle and mortar.Afterward, powder (4 g) was steeped in distilled water (400 mL) for 2 weeks.
The mixture was filtered using Whatman filter paper to separate the soluble part from the insoluble portion.The filtration procedure was repeated three times for effective collection of extract.Later, the collected extracts were used for further analysis and biosynthesis of nanoparticles.

| Phytochemical analysis
The resultant extract was evaluated for the presence of secondary constituents through procedures adopted by Adebayo et al. (2009).

| Alkaloids
0.5 g of P. harmala plant extract was heated in the presence of H 2 SO 4 (2%) followed by mixing with Dragendorff's reagent.The appearance of orange-red precipitates showed the presence of alkaloids.

| Tannins
0.2 g of P. harmala plant extract was boiled and filtered.Ferric chloride was added dropwise in each filtrate.The presence of tannins was indicated through the appearance of a dark green color.

| Anthraquinones
0.5 g of P. harmala plant extract was heated in HCl (10%) for a short duration.The heated solution was cooled followed by filtration and the addition of chloroform.Ammonia (10%) was mixed with each mixture and heated again.The appearance of pink color revealed the presence of anthraquinones.

| Glycosides
Each extract was hydrolyzed using HCl and further neutralized with NaOH solution.Fehling's solution (A: 1 mL, B: 2 mL) was added in small amounts to each extract (2 mL) and boiled.The appearance of crimson precipitates revealed the presence of glycosides.

| Saponins
0.5 g of P. harmala's extract was mixed with distilled water followed by boiling.Foaming revealed the presence of saponins in each extract.
2.3.6 | Test for carbohydrates 0.5 g of P. harmala's extract was boiled in a water bath with Fehling's solutions A and B. Formation of brick red color revealed the presence of carbohydrates.

| Test for soluble starch
5% KOH was added to a small amount of each extract before being heated, cooled, and acidified with H 2 SO 4 .It was assumed that a yellow hue indicated the existence of soluble starch.

| Flavonoids
About 0.2 g of crude extracts were dissolved in dilute NaOH with a few drops of HCl.Flavonoids were found in both extracts as evidenced by the observation of a yellowish solution that became colorless when HCl was added.

| Phlobatanins
About 0.5 g of crude extracts were diluted in distilled water and then filtered.With HCl (2%) solution, the filtrates were heated until they boiled.No red precipitate crystallized, which was a sign that phlorotannins weren't present.

| Steroids
0.5 g of plant extracts were added together with 2 mL of H 2 SO 4 and acetic anhydride.The presence of steroids was established by the creation of blue color from violet.

| Terpenoids
To create a layer, 0.2 g of crude plant extracts, 2 mL of chloroform, and 3 mL of pure H 2 SO 4 were carefully combined.The interface developed a reddish-brown hue, indicating the presence of terpenoids.

| Test for coumarin
Each extract's (2 mL aqueous solution) volume was increased by 3 mL of 10% NaOH.Yellow color development indicated the presence of coumarin.

| Test for emodin
After combining 0.5 g of the crude extracts with 2 mL of NH 4 OH, 3 mL of benzene was added.The appearance of red color indicated the existence of emodin.

| Test for anthocyanin and betacyanin
Using a gas hob, 0.2 g of plant extracts and 1 mL of two regular solutions (2 N) NaOH were combined and cooked for 5 min at 100°C.
Anthocyanins were found to be present because of the production of a bluish-green color, while betacyanins were shown to be present because of the formation of a yellow color.

| Reducing sugars
Filtering was done after shaking crude extracts with distilled water.
The filtrates were mixed with a few drops of Fehling's solutions A and B before the mixtures were heated for a short period of time.
The presence of reducing sugars was demonstrated by the presence of orange-red precipitates.

| Green synthesis of NPs
2.4.1 | Green synthesis of AgNPs using aqueous extract of P. harmala 2 mg of extract was dissolved in 100 mL distilled water to prepare stock solution.We used distilled water to make 1 mM AgNO 3 solution.Silver and extract solution were mixed in various ratios to make different fractions of the preparations such as 5:1, 6:1, 7:1, 8:1, and 9:1.The fraction was made in vials and stirred.The production of nanoparticles is indicated by a change in color in each vial's reaction mixture.For the confirmation of silver nanoparticle synthesis, the solutions were tested with a UV-visible spectrophotometer.The best preparation of AgNPs was found at a 7:1 ratio according to the UV-visible spectrophotometer results.Characterization and other analyses of the produced silver nanoparticles were also performed.

| Green synthesis of AuNPs from aqueous extract of P. harmala
In the descriptive experiment, 1 mM aqueous chloroauric acid (HAuCl 4 ) solution was added to the stock aqueous solution of leaves extract in different ratios of 2:1, 3:1, 4:1, 6:1, 7:1 and stirred/stimulated on magnetic stirrer continuously for 12 h.The leaf extract reduced the gold ions into gold nanoparticles.The nanoparticle formation was visually confirmed by the modification in color and by measuring with UV-A visible spectrophotometer in the wavelength range 450-800 nm for gold nanoparticles.The optimal AuNP preparation was found to be 4:1.Instruments like the FTIR, SEM, and EDX were used to characterize AuNPs.

| Optimization
The synthesis of silver and gold nanoparticles was examined under different temperatures, salts, pH, and different concentrations of salt (NaCl) to determine the optimum values of all these.
Additionally, spectrophotometric measurements were made of the effects of various salts, NaCl, temperature, and pH on the synthesized NPs.The impact was assessed using UV-visible spectra after the various molar solutions of NaCl and other salts were produced.
By maintaining the plant ratio constant and vice versa, the impact of an increase in the ratio of gold and silver NPs colloidal was also assessed.At each of the three pH levels-acidic, basic, and neutral-the impact of the pH shift was also assessed.Utilizing NPs at various temperatures allowed researchers to evaluate the impact of temperature.

| Characterization of Ag and Au NPs
P. harmala extracts were introduced for 2-12 h at a specific temperature in a solution containing Ag and Au salts.The change in color indicated the synthesis of nanoparticles.The Ag and Au nanoparticles generated by green synthesis were characterized using several characterization techniques.The UV-visible spectroscopy approach was used to validate the nanoparticle production technique; the FTIR technique was utilized to assess group stability, and the EDX technique was used to assess composition.SEM is used to measure the size.

| UV-Visible spectroscopy
To examine nanoparticles, a wavelength range of 200-800 nm was utilized.By developing a ruby-red hue, AgNPs were visually verified to have formed.To verify the creation of the AuNPs, the absorbance was scanned at wavelengths between 200 and 800 nm.Quartz cuvettes with an optical path 1 cm long were used for the spectroscopic studies of the silver and gold nanoparticles.

| Scanning electron microscopy (SEM)
Using an S-4800 JEOL scanning electron microscope from the University of Peshawar, the sizes and morphological surfaces of nanoparticles were measured.

| Fourier transform infrared (FTIR) spectrometric
Using a Shimadzu FTIR -8400-S (Bacha Khan University Charsadda) Fourier transform spectrometer, the detection of functional groups present in green synthesized AuNPs and AgNPs was assessed.Two optically circular flat plates were used to hold the prepared samples.
The signals picked up by the computer revealed the outcomes.The frequency range was between 500 and 4000 cm −1 .

| Energy-dispersive X-ray (EDX)
The geometry and morphology of Au and AgNPs were also determined.The preparation and identification of the colloids of Au and AgNPs in energy-dispersive X-ray spectroscopy was done using a Bruker X-flash.The electron beams 15 keV energy was maintained for EDX and imaging.

| Biological assays
Used the disc diffusion method to determine the pharmacological of the extract and synthesized nanoparticles.These activities were conducted in the Department of Microbiology, University of Hazara.

| Antibacterial activity
The zone of inhibition of AgNPs and AuNPs was determined in the microbiological activity.Staphylococcus aureus and Escherichia coli were the bacteria used in the microbiological experiment.Micro dilutions were used to create the NPs.Nutrient agar was used to create the bacterial culture, which was then incubated for 24 h at 37°C.The NP-containing petri plates were similarly incubated for 24 h at 37°C.AuNPs and AgNPs had their MBC measured.The region of inhibition was tested using the lowest doses or dilutions of Au and AgNPs.To acquire accurate findings, the procedure was performed three times.

| Antifungal activity
The fungicidal activity was determined using the microdilution plate test technique.In the test, Candida albicans was employed as the fungus.A 20 mM sodium phosphate buffer solution was combined with 20 L of gold and silver nanoparticle dilutions of 5, 2.5, and 1.25 mg/mL in water.The sample was incubated for 2 h at 37°C.

| Phytochemical screening
For phytochemical screening, the crude extract and different fractions were evaluated.The MeOH extract indicated the presence of tannins, anthraquinones, alkaloids, saponins, anthocyanins, phlobatanins, steroids, coumarins, betacyanins, proteins, and combined reducing sugars.In n-hexane extract, the presence of reducing sugar, steroids, and terpenoids were indicated.The aqueous extract showed the presence of saponins, terpenoids, combined reducing sugars alkaloids, emodins, anthraquinones, phlorotannins, and coumarins.Normally the polar secondary metabolites were found in all polar extracts.The phytochemical profile of the selected plants is represented in Table 1.

| Synthesis of silver and gold nanoparticles Using P. Harmala leaves extracts
In the current study, P. harmala leaves extract was taken for the reduction purpose of AgNO 3 to synthesize silver nanoparticles.For the synthesis of Au nanoparticles, the P. harmala extract was mixed with a solution of HAuCl 4 •3H 2 O and incubated with different salt solutions.For this determination, aqueous extract solution was used and mixed for 24 h.After mixing the solutions, the silver nitrate solution color is changed to brownish color as shown in Figure 1.

| Optimization of various parameters for synthesis of silver nanoparticles
Initially, numerous factors were used to optimize the silver nanoparticle synthesis.During the experiments, the solution of AgNO 3 and leaves extract were reacted in various ratios (5:1, 6:1, 7:1, 8:1, and 9:1).Gradual changes in color from yellow to reddish yellow and from reddish yellow to dark brown were observed for aqueous solution mixture of AgNO 3 and leaves extract of the plant.The whole conversion of the aqueous solution from yellowish brown to blackish brown designates the complete formation of silver nanoparticles (AgNPs).

| UV-Visible spectroscopy of Ag NPs
The full bio-reduction of silver ions to silver nanoparticles in samples from the various mixtures of silver nitrate solution and plant extract was observed.By scanning a UV-visible spectrophotometer, silver nanoparticles were initially characterized.
The varying ratios of the reaction mixture (1:1 to 9:1) displayed various strengths of the absorption bands, demonstrating that the plant extract was used to create the silver nanoparticles (Figure 2).
The highest surface plasmon absorption band, which is in the absorbance range (400-500 nm) for silver nanoparticles, was provided by an 8:1 ratio at a maximum of 425 nm.The instantaneous transformation of the substance's color from creamy yellow to brownish, which over time becomes entirely dark reddish brown, shows that the creation of silver nanoparticles began.According to UV-visible spectrophotometry, the reaction mixture's brownish color of silver nanoparticles is caused by the stimulation of surface plasmon resonance.

| Effect of salt concentration on silver nanoparticles
The impact of varying ionic strengths on the size of silver NPs was also investigated.Because the important environmental circumstances may change depending on the salts and their ionic strength, examination of NPs in different salts ranges of ionic strength is also required for assessment of NP behavior in diverse salt environments.The salts used in the evaluation of the effects on silver NPs  were placed in beakers from left to right.The greatest peak was detected at 440 nm wavelengths for a pure sample with an absorbance of 18.8 a.u., according to UV-visible spectroscopy measurements.
After a pure sample at 430 nm wavelength with an absorbance value of 1.45 a.u., the highest peaks are detected at pH 8 and pH 14.In a spectrum, the solution with pH 8 and 14 had more steady peaks, indicating excellent nanoparticles.The AgNPs were stable at basic conditions.However, the absorption reduced when the pH dropped below 6.0.At higher pHs, we also noticed narrow peaks and strong absorption.All reaming pH solutions, with the exception of these two, showed a decrease in absorbance in the 350-700 nm regions as shown in the inset of Figure 5.

| Effect of NaCl on silver nanoparticles
The silver nanoparticle solution was combined with NaCl at varied ratios of 0.01, 0.1, 0.2, 0.3, 0.4, 0.5, and 1.0 to see what effect it had.
As the concentration ratio rises, the color shifts from left to right.
The spectra of these solutions were obtained using UV-visible spectroscopy.When the salt concentration was increased, the absorbance value at 420 nm wavelength drops, according to the UV-visible spectrum.The 0.01 concentration solution, as it can be concluded, has a higher absorbance value than the other concentrations.At 0.5 and 1.0 concentrations, the absorbance values are nearly identical.
However, the graph shows that the NaCl treatment reduces the intensity of the peaks as shown in Figure 6.The prominent color was dark blue as shown in Figure 7.

| UV-Visible spectroscopy of gold nanoparticles
Following the addition of P. harmala plant extract, the colorless gold chloride solution quickly turned to purple, indicating that a reaction had occurred and the gold ions had been converted to gold nanoparticles.This was only a visual cue concerning the creation of nanoparticles, which was later validated by UV-visible spectrophotometry.The maximum absorbance peak was seen at 535 nm for gold nanoparticles.Only the 4:1 ratio sample exhibited accurate stability results in all tests, according to the UV-visible spectrum.All of the peaks were virtually visible in the 500-600 nm range, as indicated in Figure 8, and aqueous solutions in different ratios.

| Salt effect on gold nanoparticles
The impact of various ionic strengths on the size of gold NPs was also As can be seen from the graph's steep gradient, gold nanoparticles are more vulnerable to high salt strength than silver nanoparticles.
The formation of aggregates in the medium is indicated by the size increase of nanoparticles.

| Heat effect on gold nanoparticles
The est peaks are at 30, 40, and 60°C, as illustrated in Figure 10.The findings reveal that the nanoparticles are more stable at temperatures between 30 and 80°C and that it is unstable at temperatures above 80°C due to dispersion as given in Figure 10.

| pH effect of gold nanoparticles
This study shows that the production of gold nanoparticles is better suited to alkaline pH 8 and 12.The pH effect is shown in Figure 11.

| Effect of NaCl on gold nanoparticles
The gold nanoparticles made from leaf extracts were treated with varied amounts of sodium chloride (NaCl).UV-visible spectroscopy was used to investigate the effects of various solutions.Figure 12 depicts how the color of the sample changed at each concentration.For concentrations of 0.1, 0.5.1.0,1.5, and 2.0, a UV-Visible spectrum was obtained.For all values between 2.25 and 2.3 a.u., the absorbance value is essentially identical.Furthermore, compared to the pure sample figure, all peaks for all samples show a decrease as shown in Figure 12.

| Fourier transform infrared (FTIR)
The FTIR spectroscopy is commonly used for showing the presence of functional groups of bio-molecules in the extract that behave as reducing and capping agents.containing alcohol functional groups, and carboxylic compounds presented in the P. harmala leaves extract.The high-intensity peak at 1602 cm −1 is related to C=C and C=O vibrations of benzene rings and carbonyl groups of amino acids.The peak at 1382 cm −1 is allocated to stretching vibrations of the carboxylate groups.The absorption band at 1249 cm −1 is related to hydroxyl groups in polyphenolic compounds with the in-plane bending vibration.The peak at 1034 cm −1 is point out with the C-O band stretching of flavonoids.The small shift of several bands can be due to interactivity between functional groups of biomolecules and nanoparticles.It should be noted that the peak at 1382 cm −1 in the FT-IR spectrum of capped AgNPs was strongly reduced.Accordingly, respective polyphenolic groups are proven as the main reducing agents in the synthesis of MNP.

| SEM
The P. harmala plant extracts synthesized silver nanoparticles by the green method were investigated by scanning electron microscopy to confirm the structure analysis.SEM images revealed that the structure of synthesized nanoparticles is oval, spherical, rod type and average size is from 42 to 72 nm.Similarly, the gold nanoparticles had oval and spherical forms between 12.6 and 35.7 nm, as revealed in TA B L E 2 Antibacterial activity of Peganum harmala extract, its Silver and gold nanoparticles against selected bacterial strains.

Escherichia coli Pseudomonas aeroginosa Salmonella typhi
Zone of inhibition (mm) same showed 20, 22, 23, 21, and 23 mm against the above-mentioned bacterial strains, respectively (Table 2).The control (levofloxacin) showed a 100 mm zone of inhibition (Table 2).The plant extract of P. harmala showed good activities against selected bacterial strains compared to the standard control levofloxacin, but S. typhi was found resistant to plant extract of P. harmala.
The synthesized AgNPs showed good activities against selected bacterial strains compared to the standard control levofloxacin, but P. aeroginosa was found resistant to the synthesized AgNPs of P. harmala.Similarly, the synthesized AuNPs also showed good activities against selected bacterial strains compared to the standard control levofloxacin, however B. subtilis was found resistant to the synthesized AuNPs of P. harmala as shown in Table 2 and Figure 16.

| Anti-fungal activity
The anti-fungal activity of silver and gold nanoparticles synthesized  3 and   Figure 17.

| DISCUSS ION
Medicinal plants as a whole and their associated extracts from different parts are found to be significant in treating various metabolic syndromes.Plant extract-based nanoparticles (NPs) are known as a significant approach for effective drug delivery (Abu-Tahon et al., 2020;Aljabali et al., 2018;Iqbal et al., 2020).In modern medicine, the biosynthesis of plant-based nanoparticles along with their promising bioactivities has gained significant interest of the scientific community.Plant-extract-based nanoparticles are preferred owing to their cost-effectiveness and safety (Bawazeer et al., 2022).
Literature shows that scientists have been biosynthesizing silver and gold nanoparticles from extracts of various plant parts like seeds, leaves, flower, bark, and roots (Kumar & Yadav, 2009).P. harmala extracts are found to be a rich source of phytochemicals that can be used for the treatment of various ailments.According to a study conducted by Qian et al. (2008), biosynthesized nanoparticles of P.
harmala seeds extract acted as an alternative to traditional physical

Candida albicans Aspergillus niger Penicillium notatum
Zone of inhibition (mm) F I G U R E 17 Anti-fungal activity of the synthesized AgNPs and AuNPs by using plant extracts.
In this study, varied ratios of the reaction mixture displayed various strengths of the absorption bands, demonstrating that the plant extract was used to create the silver nanoparticles.In the case of silver nanoparticles, a ratio 8:1 revealed maximum absorbance (425 nm) and indicated the presence of greater silver nanoparticles in the solution.Whereas, in the case of gold nanoparticles, sample ratio 4:1 showed stable results according to UV-Vis's spectra.
Hence, the 8:1 (AgNPs) and 4:1 (AuNPs) ratio was further used for formulation of bulk solutions and further analysis.According to the UV-Vis spectrum, both the synthesized silver and gold nanoparticles were found to be more stable in CuCl 2 as compared to other salt solutions.While, AgNPs and AuNPs were stable at 70 and 80°C, respectively.Moreover, both the AgNPs and AuNPs were most stable at pH 14.The reaction mixture's brightest color revealed the development of NPs and the absence of any reaction at low pH (Sathishkumar et al., 2010).The widening of the peaks was created at low pH ranges, which denotes the development of large-sized NPs.Alkaline pH displays the narrowing of the peak at 400 nm with maximal sharp peak formation in the extract-mediated synthesis of NPs.The development of the sharp peak is a sign that the nanoparticles' spherical shape has formed (Kumari et al., 2015).According to several studies, pH has a significant impact on how nanoparticle size and shape are controlled during synthesis.
The major bands of the P. harmala extract were recorded at 3270, 1602, 1382, 1249, and 1034 cm −1 .The broad band at 3270 cm −1 is attributed to the stretching vibration of hydroxyl groups in polyphenolic compounds, terpenoids containing alcohol functional groups, and carboxylic compounds presented in the P. harmala leaves extract.The high-intensity peak at 1602 cm −1 is related to C=C and C=O vibrations of benzene rings and carbonyl groups of amino acids (Motzer et al., 2018).The peak at 1382 cm −1 is allocated to stretching vibrations of the carboxylate groups.The absorption band at 1249 cm −1 is related to hydroxyl groups in polyphenolic compounds with the in-plane bending vibration (Odeniyi et al., 2020).The peak at 1034 cm −1 is points out with the C-O band stretching of flavonoids (Gopinath et al., 2020).
Synthesized nanoparticles are oval, spherical, and rod-type and average size is from 42 to 72 nm.Similarly, the gold nanoparticles had oval and spherical forms between 12.6 and 35.7 nm.The results of our study were similar to those reported in the literature (Yeamin, 2012).The formation of aggregates in the medium is indicated by the increase in the size of nanoparticles.The spectra's steepness depicts particle aggregation produced by ionic strength.
The impacts of various electrolytes on nanoparticles were investigated and compared; concluding that salts containing divalent cations have a greater impact on NP aggregation than salts containing monovalent cations (Baalousha et al., 2013).The aggregation of NPs was also aided by the chloride ions.The aggregation of NPs demonstrated that divalent electrolytes effectively destabilized NPs (Huynh & Chen, 2011).The graph's steepness depicts particle aggregation produced by ionic strength (Baalousha et al., 2013).
Nanoparticles investigated and compared the impacts of various electrolytes, concluding that salts containing divalent cations have a greater impact on NP aggregation than salts containing monovalent cations.The aggregation of NPs was also aided by the chloride ions.Likewise, Badawy also looked at gold nanoparticles and the effects of various monovalent and divalent cations (Badawy et al., 2010).

| CON CLUS IONS
The research work can be concluded as the best-optimized ratios for Observation of color transformation from clear to dark.F I G U R E 2 UV-Visible spectrum of silver nanoparticles synthesized by mixing leaves extract and AgNO 3 solutions in different ratios.are NiCl 2 , CuCl 2 , KCl, MnCl 2 , and PbCl 2 .The differences in the particle size as a function of the ionic strength were shown in spectra (Figure 3).
Figure 5, several pH solutions with values of 2, 4, 6, 8, 10, 12, and 14 Different optimization conditions and variables were used to optimize gold nanoparticle synthesis.HAuCl 4 .3H 2 O and P. harmala leaf extracts were combined at various ratios to examine Au F I G U R E 3 UV-Visible spectrum showing the effect of different salts on silver nanoparticles.F I G U R E 4 UV-Visible spectrum for effect of heat on Ag nanoparticle solution.F I G U R E 5 UV-Visible spectrum showing the effect of different pH on silver nanoparticles.nanoparticles.After that, these solutions were placed in various conditions for optimization.The ratios i.e., 2.1, 3.1, 4.1, 6.1, and 7.1 of these solutions were used in the experiment, and it took some time to see how the color changed when exposed to UV-visible spectroscopy.The change in color was noted in the image below.
investigated.Because the important environmental circumstances may change depending on the salts and their ionic strength, examination of NPs in different salt ranges of ionic strength is also required for the assessment of NPs behavior in diverse salt environments.The salts used in the evaluation of the effects on gold NPs are NiCl 2 , CuCl 2 , KCl, MnCl 2 , and PbCl 2 .The differences in the particle size as a function of the ionic strength are shown in Figure 9.
gold nanoparticles were then heated at different temperatures to see what happens.The solution was exposed to a range of temperatures i.e., 30, 40, 50, 60, 70, and 80°C.Only two levels of temperature, 70 and 80°C, display a maximal absorbance peak of 1.65 F I G U R E 7 Optimization of gold nanoparticles with different concentrations.F I G U R E 8 UV-Visible spectrum of gold nanoparticles synthesized by mixing leaves extracts.F I G U R E 9 UV-Visible Spectrum revealing the effect of different salts on gold nanoparticles.F I G U R E 6 UV-Visible spectrum showing the effect of different concentrations of NaCl solution on silver nanoparticles.and 1.25 a.u. at 560 nm wavelengths, as shown by the UV-visible spectroscopy results.The highest peaks are at 80°C, while the low- Figure 13 reveals the FTIR spectra of the dried extract from the P. harmala plant and Ag and AuNPs.Accordingly, the FTIR spectrum of the dried extract and synthesized Ag and AuNPs appear nearly the same in the number of characteristic peaks, and their intensity for all samples was observed.The major bands of the P. harmala extract were recorded at 3270, 1602, 1382, 1249, and 1034 cm −1 .The broad band at 3270 cm −1 is attributed to the stretching vibration of hydroxyl groups in polyphenolic compounds, terpenoids F I G U R E 1 0 UV-Visible Spectra showing effect of heat at different temperatures on gold nanoparticles.F I G U R E 11 UV-Visible spectra showing the effect of different pH solution on gold nanoparticles.F I G U R E 1 2 NaCl treatment with different concentrations in gold nanoparticle.F I G U R E 1 3 FTIR spectrum of Peganum harmala extracts, AgNPs and AuNPs.
from P. harmala leaf extracts is investigated using a microdilution technique.C. albicans and Aspergillus Niger were among the fungus species used in the experiment.The anti-fungal activity of P. harmala extracts and synthesized nanoparticles were investigated in the 43-growing medium.Antifungal activity of synthesized nanoparticles and P. harmala extracts against C. albicon (18 mm), A. Niger (16 mm), and Penicillium notatum was discovered (14 mm).Synthesized gold nanoparticle of P. harmala leaves extracts demonstrate 31, 26, and 28 mm zone of inhibition.Similarly, silver nanoparticle shows 26, 30, and 36 mm.The antifungal activity results are shown in Table and chemical approaches owing to their eco-friendly and economical effects.Moreover, Odeniyi et al. (2020) also synthesized nanoparticles using P. harmala plant extract and suggested a color change from yellow to red-brown during the formulation of silver nanoparticles.Various studies have reported the synthesis of silver nanoparticles from extracts (methanolic and aqueous) of different parts of F I G U R E 1 6 Anti-bacterial activity of the synthesized AgNPs and AuNPs by using plant extracts.Anti-fungal activity of the synthesized AgNPs and AuNPs by using plant extracts.