Scavenging effect of pasipay (passiflora incarnate L.) on singlet oxygen generation and fatty acid photooxygenation

Abstract Anthracene as a chemical probe is usually used to trap the singlet oxygen and then detection and quantification can be based on absorbance. In this study, oxidation of anthracene declared that rate of singlet oxygen quenching in the presence of pasipay (passiflora incarnate L.) as a natural antioxidant, 1,4 Diazabicyclo [2.2.2] octane (DABCO) as a well‐known singlet oxygen scavenger and highly effective synthetic antioxidants in food industry such as Butylated hydroxytoluene (BHT), Butylated hydroxyanisole (BHA), tert‐Butylhydroquinone (TBHQ) decreased in the order of DABCO >pasipay > TBHQ > BHT > BHA. On the other hand, lipid photooxidation is the undesirable chemical process in which singlet oxygen result in the peroxidation of fatty acids. The results of this study also showed that oleic acid oxidation with singlet oxygen in the presence of pasipay (contains 0.4576 mg flavonoid compounds) diminished about 11% which shows pasipay has an effective role to inhibit lipid peroxidation.


| INTRODUC TI ON
Oxygen molecule in its ground state has two unpaired electrons and when oxygen molecule has excess energy, these unpaired electrons in the external orbital can be pair and generate singlet oxygen (Min & Boff, 2002). One of the physical methods for producing singlet oxygen is photosensitization reaction. Great photosensitizers have received attention, due in part to their direct relevance to many biological systems. Electrophilic tendency of singlet oxygen causes lipids, amino acids, nucleic acids, and electron rich molecular can be its target (Korytowski, Schmitt, & Girotti, 2010).
Singlet oxygen can be easily produced in food systems under light illumination, especially in the presence of photosensitizers such as riboflavin and chlorophylls (Greer, 2006). Lipids also can be a target of singlet oxygen due to the electrophilic inherent, this reactive species attacked to unsaturated fatty acid and produce lipid hydroperoxides as primary products (Girotti, 1998). On the other hand UV radiation causes DNA damage and protein oxidation and induces the synthesis of matrix metalloproteinases (Kligman, 1989). The use of antioxidants to protect human skin from the harmful effects of UV radiation is a topic that has attracted growing interest in recent years within the world of photoprotection research (Martinez et al., 2017). DABCO recognized as a very efficient quencher of singlet oxygen in the organic media (Lengfelder, Cadenas, & Sies, 1983) and synthetic antioxidants such as TBHQ, BHA, and BHT have been found to have a strong singlet oxygen quenching ability (Lee & Jung, 2010). Recent research has focused on isolation and characterization of effective natural antioxidants (Fang et al., 2017;Nimse & Pal, 2015). Natural antioxidants act (a) as reducing agents, (b) as free radical scavengers, and (c) as quenchers of the formation of singlet oxygen. They can be used in the food industry and there is evidence that they may exert their antioxidant effects within the human body (Hamid, Aiyelaagbe, Usman, Ameen, & Lawal, 2010;Koski et al., 2002). People receive antioxidant supplements directly from fresh fruits and vegetables.
The World Health Organization estimated that <80% of the earth's | 1671 HAJIMOHAMMADI AnD nOSRATI inhabitants rely on traditional medicine for their primary health care needs and most of this therapy involves the use of plant extracts or their active phenolic components (Bruneton, 1995) which have an efficient antioxidant capacity. Pasipay is the largest and most important genus of the family Pasipayceae, comprising about 500 species, distributed mostly in warm temperate and tropical regions (Dhawan, Dhawan, & Sharma, 2004) Previous studies have described the presence of flavonoids as the major constituents of pasipay (Dhawan, Dhawan, & Sharma, 2004). There are few studies on the efficacy of natural antioxidant as O 2 ( 1 Δg) quenchers and their roles in the prevention of lipid oxidation (Niki, 2015;Terao, Minami, & Bando, 2010) because scavenging of DPPH free radical is the basis of a common antioxidant assay and most often an overall antioxidant effect was measured. However, singlet oxygen has not radical nature. (Ruiz-González et al., 2017) This project was designed to characterize the antioxidant potential of passiflora incarnate L. as a natural antioxidant in compare with well-known singlet oxygen scavenger such as DABCO and the highly effective antioxidants such as BHA, BHT, and TBHQ.

| Sample preparation for anthracene oxidation with singlet oxygen
In a typical experiment, 15 ml acetonitrile, 0.002 mmol antioxidant (DABCO, BHT, BHA, TBHQ, and pasipay (contains 0.5731 mg flavonoid) separately, were added to anthracene (4 × 10 −4 M) and MB (1 × 10 −4 M). Continuous irradiation of samples was carried out using solar simulator light (288 power LED lamps, 1 W, 2.3 V (59660 LUX)) for 5 min at room temperature under 1 atm of bubbling of air in the solution at room temperature. Determination of products was recorded on a Shimadzu 2100 spectrophotometer at 375 nm.

| Statistical analysis
In all analyses, three replicates were applied and analysis of the results was achieved using SAS software, version 3.9 and then average the results were compared using Duncan test. Also, with Excel software diagrams were drawn.

| Evidences for singlet oxygen generation and biocidal activity of pasipay in the photooxidation of oleic acid
In this work, the oxidative alterations of oleic acid as a result of oxidation with singlet oxygen were analyzed in the presence and absence of pasipay as a source of natural phenolic compounds. Our target was fatty acid oxidation with singlet oxygen as a noble species F I G U R E 1 Oleic acid photooxygenation in the presence and absence of Pasipay (a). Structure of different applied photosensitizers (b) which has worked few studies on it (Terao, Minami, & Bando, 2010).
Photooxygenation of oleic acid with H 2 TPP as a photosensitizers was investigated as a typical standard sample to evaluate singlet oxygen production (Figure 1).
It is important to note that 1 H NMR spectroscopy and iodometric method revealed oxidation of oleic acid to peroxide product stopped in the absence of photosensitizers (Figure 2 and Table 1 entry 1) or when the irradiation was interrupted (Table 1 entry 2).
Accordingly, the presence of a H 2 TPP, light and O 2 are essential for the conversion oleic acid to corresponding products (Table 1 entry 3).
According to the literature, there are two major pathways for photooxygenation reactions in the presence of nonmetal photosensitizers, Type I and Type II (Figure 3a) (Laing, 1989).
Singlet oxygen generation (Type II) and its reaction with the substrates is the foremost mechanism that occurs in our circumstances, since conversions of oleic acid obey the order of H 2 TPP > ZnTPPCl > MgTPPCl (Table 1 entry 3, 4, and 5).
Paramagnetic metals are claimed to quench singlet oxygen by energy transfer mechanism from oxygen to the low-lying d orbital electron levels and have very short triplet lifetimes (  (Bressan & Morvillo, 1989;Chen et al., 2001;Toffoli, Gomes, Junior, & Courrol, 2008). Table 1 entry 3, 7, and 8 indicates that conversion of oleic acid in acetonitrile as solvent is higher than ethanol and DMSO that correlated with singlet oxygen lifetimes in this solvents. For investigation of the type I mechanism, we performed our reaction in the presence superoxide anion radical (O 2 •-

| Effect of pasipay on Anthracene photooxygenation
Spectrophotometry is a more convenient option for detection of excited oxygen molecules. A chemical probe is usually used to trap the singlet oxygen and then detection and quantification can be based on absorbance. A very characteristic reaction of singlet oxygen is the [4 + 2] cycloaddition to conjugated cyclic dienes and polycyclic aromatic hydrocarbons such as anthracene (Aubry, Pierlot, Rigaudy, & Schmidt, 2003). Anthracene traps reversibly singlet oxygen. Singlet oxygen generation by MB is evidenced by chemical trapping of 1 O 2 with anthracene. The UV-Vis spectra of anthracene as the function of time irradiation by using of MB as a photosensitizer are displayed in Figure 4a. A reduction of the emission intensity absorption band of anthracene (λmax = 375 nm) was observed with the increase in irradiation time. This response is a consequence of the anthracene-9,10endoperoxide formation (see Figure 4). During the reaction, the addition of well-known singlet oxygen scavenger such as DABCO and highly effective synthetic antioxidants in food industry in foods such as BHT, BHA, TBHQ, and pasipay inhibited the oxidation of anthracene in the order of DABCO > pasipay > TBHQ > BHT > BHA (Figure 4a,b). Moreover, the oxidation reaction did not occur under dark conditions. This confirms that the anthracene oxidation occurs by singlet oxygen under visible irradiation. These results show pasipay act as a very effective deterrent on singlet oxygen generation.

| Effect of pasipay on fatty acid photooxgenation
The photosensitized production of singlet oxygen has significance in the areas of the photooxidation of organic compounds (Hajimohammadi, Schwarzinger, & Knör, 2012) and food chemistry [1, 2, 4]. Photooxygenation of oleic acid as a one of the targets of singlet oxygen was investigated to evaluate the antioxidant effect of pasipay. Figure 5 shows the conversion of oleic acid to the peroxide products in an oxygenated solution of acetonitrile and H 2 TPP photosensenisitizer under visible light in the presence of pasipay, well-known singlet oxygen (DABCO) and highly effective antioxidants such as BHT, BHA and TBHQ.
After 120 min irradiation, the rate of oleic acid oxidation by 1 O 2 as a very reactive ROS reduced 11.6% in the presence of pasipay (contains 0.4576 mg flavonoid compounds) that shows pasipay can be used as an effective additive to fatty acids for the preservation of them. Also, the rate of oleic acid preservation in the presence of these types of antioxidants decreased in the order of TBHQ > DABCO > BHT > BHA > pasipay.
Herbal and natural source of flavonoid and polyphenol compounds have been reported to act as scavengers of various TA B L E 1 PV of oleic acid oxidation with singlet oxygen in different condition a

| CON CLUS ION
Due to the increase of diseases such as cancer, Alzheimer's disease, skin disorders, etc. with ROS especially singlet oxygen and light, finding efficient antioxidant is very important. In this study, antioxidant capacity for pasipay and synthetic polyphenolics against singlet oxygen was comprehensively assessed by anthracene oxidation assay and evaluation of fatty acid oxidation. It was showed pasipay has an effective role in restricting of singlet oxygen generation and limitation of fatty acid photooxidation.

ACK N OWLED G M ENT
We gratefully acknowledge support from the Kharazmi University.

CO N FLI C T S O F I NTE R E S T
The authors declare that they have no conflict of interest.

E TH I C A L S TATEM ENT
This article does not contain any studies with human participants or animals performed by any of the authors.