Procalcitonin as a prognostic marker of patients with acute ischemic stroke

Abstract Background The information on mortality after an acute stroke patient is still limited. Objectives The aim of this study was to investigate the prognostic potential of procalcitonin (PCT) serum levels in acute ischemic stroke. Methods A total of 748 patients were enrolled in this study. Prognostic potential of PCT was evaluated by Kaplan‐Meier analysis and multivariable Cox hazard regression analyses. Results Procalcitonin levels were found to be significantly higher in acute ischemic stroke patients who died in 30 days than those who survived. Univariate logistic regression analysis showed that PCT was significantly associated with 30‐day mortality, and Cox regression analysis revealed that PCT was a strong predictor of 30‐day overall mortality. Kaplan‐Meier analysis revealed that overall cumulative 30‐day mortality was significantly higher in those with PCT levels >1.5 ng/mL when compared to those with PCT levels <1.5 ng/mL. Conclusions Procalcitonin is a significant independent prognostic marker of 30‐day mortality after the one set of acute ischemic stroke.

December 2018 in a tertiary hospital. Acute ischemic stroke defined according to the World Health Organization criteria. 12 We excluded patients with intracerebral hemorrhage, subarachnoid hemorrhage, systemic infections, transient ischemic attack, inflammation, and incomplete data.
For PCT measurement, the blood sample was collected by venipuncture within 48 hours after hospital admission. Blood samples were centrifuged at 2264 g for 10 minutes. The serum was measured within 2 hours after sample collected. PCT was measured in serum sample (Cobas e601, Roche). All-cause of mortality defined as death occurring after hospital admission. All clinical data were collected from electronic medical records.
All data analyzed using SPSS 22.0. Two groups of any continuous variables were compared using Student's t test. Categorical variables were compared between groups using the chi-square test. Survival analysis by multivariable Cox regression analysis was used to examine the risk factors for 30-day mortality. The survival distribution function was estimated with the Kaplan-Meier method, and a non-parametric log-rank test was used to compare the survival curves among the different groups. P < .05 was considered statistically significant.

| RE SULTS
A total of 748 patients were enrolled in this study. The median age was 68 years, with age ranged between 46 and 101. Demographic data, risk factors, laboratory finding, and 30-day outcome were shown in Table 1. The inflammatory biomarkers including PCT, CRP, and neutrophils percentage in patients who died in 30 days were significantly higher than those who survived. Furthermore, the serum levels of PCT were also higher in patients with unfavorable functional outcome than those in patients with favorable functional outcome 1.37 ± 1.81 ng/mL vs 0.12 ± 0.41 ng/mL. Death by the 30-day follow-up was associated with total bilirubin, urea, neutrophil percentage, and CRP (Table 2). Hypertension, coronary heart disease, total bilirubin >17 μmol/L, and urea >7.0 mmol/L were not associated with the mortality.
Multivariate Cox regression analysis was used to analyze the independent risk factors for 30-day mortality. As shown in Table 3  Abbreviations:CRP, C-reactive protein, PCT, procalcitonin.

| D ISCUSS I ON
Stroke is the first leading cause of disability and over two-thirds of stroke deaths worldwide occurring in developing countries. 13 Inflammation may play an important role in the progression of stroke, and inflammatory molecules might become both biomarkers and therapeutic targets for stroke management. 14 The infection markers and inflammatory molecules, such as CRP, WBC, and IL-6 were suggested to be biomarkers for outcome of ischemic stroke. [15][16][17] Compared with these markers, PCT was not a widely diagnostic marker, it is considered to be the best available biomarkers to diagnose infection. 18 In a recent study, PCT was suggested to be an independent risk factor for ischemic stroke. 19 However, the prognostic value of serum PCT in acute ischemic stroke was little to know. 10 In his study, our results showed that the concentrations of PCT in patients who died in 30 days were significantly higher than those who survived. As shown by multivariate logistic regression PCT level was an independent prognostic marker of 30-day mortality. And Kaplan-Meier analysis indicated PCT levels might help to predict 30-day mortality. These results suggested that the concentration of PCT strongly predicted all-course mortality in patients with acute ischemic stroke in first 30 days. However, hypertension and coronary artery disease, which could increase the incidence of ischemic stroke, 20,21 were not associated with the 30day mortality of ischemic stroke in our data. Moreover, in our data, PCT levels were also significantly higher in patients with unfavorable functional outcome compared with those in patients with a favorable outcome, which was consistent with previous report. 22 Procalcitonin was a specific inflammatory biomarker, and systematic inflammation plays a crucial role in the pathogenesis of ischemic stroke. Thus, the increased PCT level in serum may due to the inflammatory process in acute ischemic stroke. The increased level of PCT was also observed after severe trauma, 11 so we suspected that the increased level of PCT in acute ischemic stroke may also partly because the tissue injury after stroke. In this study, we found that PCT level was associated with the 30-day mortality in Chinese patients, this may indicate that PCT was an independent prognostic marker of short-term mortality after one set of ischemic strokes.