Autoantibody of NRIP, a novel AChR‐interacting protein, plays a detrimental role in myasthenia gravis

Abstract Background Nuclear receptor interaction protein (NRIP) co‐localizes with acetylcholine receptor (AChR) at the neuromuscular junction (NMJ), and NRIP deficiency causes aberrant NMJ architecture. However, the normal physiological and pathophysiological roles of NRIP in NMJ are still unclear. Methods We investigated the co‐localization and interaction of NRIP with AChR‐associated proteins using immunofluorescence and immunoprecipitation assay, respectively. The binding affinity of AChR‐associated proteins was analysed in muscle‐restricted NRIP knockout mice and NRIP knockout muscle cells (C2C12). We further collected the sera from 43 patients with myasthenia gravis (MG), an NMJ disorder. The existence and features of anti‐NRIP autoantibody in sera were studied using Western blot and epitope mapping. Results NRIP co‐localized with AChR, rapsyn and α‐actinin 2 (ACTN2) in gastrocnemius muscles of mice; and α‐bungarotoxin (BTX) pull‐down assay revealed NRIP with rapsyn and ACTN2 in complexes from muscle tissues and cells. NRIP directly binds with α subunit of AChR (AChRα) in vitro and in vivo to affect the binding affinity of AChR with rapsyn and rapsyn with ACTN2. In 43 patients with MG (age, 58.4 ± 14.5 years; female, 55.8%), we detected six of them (14.0%) having anti‐NRIP autoantibody. The presence of anti‐NRIP autoantibody correlated with a more severe type of MG when AChR autoantibody existed (P = 0.011). The higher the titre of anti‐NRIP autoantibody, the more severe MG severity (P = 0.032). The main immunogenic region is likely on the IQ motif of NRIP. We also showed the IgG subclass of anti‐NRIP autoantibody mainly to be IgG1. Conclusions NRIP is a novel AChRα binding protein and involves structural NMJ formation, which acts as a scaffold to stabilize AChR–rapsyn–ACTN2 complexes. Anti‐NRIP autoantibody is a novel autoantibody in MG and plays a detrimental role in MG with the coexistence of anti‐AChR autoantibody.


Introduction
The nuclear receptor interaction protein (NRIP, also named DCAF6 and IQWD1) consists of 860 amino acids with seven WD40 repeats and one IQ motif. 1,2 NRIP regulates muscle contraction via interaction with calmodulin (CaM) through the IQ motif to activate calcineurin and CaM kinase II signalling. 3,4 Muscular NRIP is also a trophic factor retrogradely supporting spinal motor neuron via the stabilization of neuromuscular junction (NMJ) by myogenin expression. 5 In addition, NRIP contributes to muscle regeneration upon muscle injury during myogenesis and myotube formation. 4 Subsequently, both global NRIP knockout and muscle-restricted NRIP knockout mice showed impaired motor behavioural performances. 4,5 In patients with limb-girdle muscular dystrophy, NRIP expression is reduced in dystrophic muscles. 6 Therefore, NRIP is a muscular protein playing an important role in maintaining normal neuromuscular integrity and function.
Myasthenia gravis (MG) is an autoimmune-related neuromuscular disorder resulting from an attack of NMJ by autoantibodies. The NMJ injury leads to muscle weakness, difficulty in ambulation, respiratory failure or even death. 7 A diagnosis of MG is essentially based on clinical presentations such as ptosis, diplopia, dysarthria and weakness of the four limbs and laboratory tests, including the presence of MG autoantibodies and electrophysiological studies. 8 The treatment for MG targets the immune system (immunosuppressant or immunomodulation) and NMJ (cholinesterase inhibitors). 9 However, some patients with MG show low therapeutic responses, and discovery of more effective and specific treatment is needed. 10 We have previously demonstrated that muscular NRIP co-localized with a major NMJ protein, acetylcholine receptor (AChR). 5 Deprivation of NRIP in skeletal muscles reduced the NMJ area with denervation of AChR. 5 NRIP probably plays a specific physiological role on NMJ. Besides, the anti-AChR antibody is the most common autoantibody (75%-80%) in MG, followed by anti-muscle-specific kinase (MuSK) and anti-lowdensity lipoprotein receptor-related protein 4 (LRP4), respectively. However, 10% of MG patients are seronegative, 10 which indicates the existence of certain unknown NMJ components with unknown autoantibodies for MG. We hypothesized that NRIP is an essential component in NMJ and anti-NRIP autoantibody contributes to the pathophysiology of MG. Using NRIP knockout cultured cells and muscular restricted NRIP knockout mice, we demonstrated that NRIP is a novel AChR-interacting protein and NMJ component protein, which acts as a scaffold to stabilize AChR-rapsyn-αactinin 2 (ACTN2) complexes. By analysing sera from patients with MG, we showed that anti-NRIP autoantibody is a novel autoantibody and plays a deleterious role in MG.

Immunoprecipitation
Equal number of protein lysates was incubated with immunoprecipitation buffer (radioimmunoprecipitation assay buffer without 1% NP-40, 1% protease inhibitor and 1% phosphatase inhibitor cocktails) and primary antibodies at 4°C overnight with constant mixing. 50 μL protein G Sepharose beads (GE Healthcare Life Sciences, Chicago, IL, USA) were added to the lysates and incubated at 4°C for 2 h and centrifuged at 8000 rpm, 1 min, 4°C, and washed with immunoprecipitation buffer twice. The target proteins were eluted by 5× sample dye and performed Western blot subsequently.

Recombinant proteins from bacteria
The GST and GST-AChR fusion proteins were expressed in Escherichia coli Rosetta (Merck, Darmstadt, Germany) cells at 16°C. According to the manufacturer's procedures, further purification was performed using glutathione Sepharose beads (GE Healthcare Life Sciences). His-tagged NRIP protein was purified by Ni-NTA agarose beads (Qiagen, Hilden, Germany). The eluted proteins were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Coomassie blue staining.

Patients
Forty-three MG patients were included in this study. The diagnosis of MG was based on typical clinical manifestations such as ocular symptoms (diplopia and ptosis), dysarthria/ dysphagia or proximal-predominant weakness with abnormal results in electrophysiological tests (repetitive stimulation test or single-fibre electromyography) or high serum titre of anti-AChR autoantibody. 8 The patients were classified according to the Myasthenia Gravis Foundation of America Clinical Classification (MGFACC). 11 The Class I MG was defined by patients only having ocular symptoms such as diplopia or ptosis, and all other muscle strength is normal. The Class II, III and IV MG were defined by patients having mild, moderate and severe weakness affecting other than ocular muscles, respectively. Class V MG was defined by patients receiving intubation with or without mechanical ventilation, except when employed during routine post-operative management. The MG was also classified into the ocular and general type or presence of thymoma or not. Respiratory support was defined using bilevel positive airway pressure (BiPAP) or requiring intubation. The stationary MG was defined as no add-on or titration of medication specifically for MG in the past 1 year. Patients were also classified according to whether they were ever hospitalized for MG except for hospitalization for MG diagnosis or thymectomy. Neurologists who took care of MG patients and collected blood samples were blinded to the test results for anti-NRIP autoantibody.

Statistical analysis
All the data analyses and graphs were performed using Microsoft Excel (Microsoft Corp.) and GraphPad Prism (GraphPad Software). Data were presented as mean ± standard deviation, except for the protein binding affinity experiment (mean ± standard error). Results were analysed by Student's t-test or Mann-Whitney U test for continuous variables and Fisher's exact test for categorical variables. The Pearson correlation coefficient r analysed the correlation. P-value of less than 0.05 was considered statistically significant.

Study approval
All animal experiments were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at the College of Medicine, National Taiwan University. All experimental mice were housed in the animal centre under a 12-h light-dark cycle with free access to food and water. The human study was conducted with the approval of and under the guidelines of the institutional review board (201811017RIND) in National Taiwan University Hospital and the written informed consent was obtained from all participants or their family members.

NRIP is a structural component of NMJ complexes
AChR is a primary structural component of post-synaptic NMJ; AChR, rapsyn and ACTN2 form a functional complex. 12 To examine whether NRIP localizes at NMJ with AChRrapsyn-ACTN2 complex in muscle, immunofluorescence assay was performed in paraffin sections of gastrocnemius muscle from 16-week-old wild-type mice. Using α-BTX (for AChR), anti-NRIP, anti-rapsyn and anti-ACTN2 antibodies, we demonstrated that NRIP is well co-localized with AChR, rapsyn and ACTN2 ( Figure 1A-C).
To confirm the association between NRIP and AChR in the muscle tissue and cultured myocytes, we performed the biotin-labelled BTX pull-down assay. The protein lysates from gastrocnemius muscles of wild-type mice were incubated with biotin-labelled BTX, pulled down by streptavidin-coupled agarose beads. The BTX pull-down component contains NRIP, rapsyn and ACTN2, indicating that NRIP is associated with the AChR-rapsyn-ACTN2 complex ( Figure 1D). Besides, in a post-differentiative cultured myocyte cell line, C2C12, the BTX pull-down component also contains NRIP, rapsyn and ACTN2 ( Figure 1E). Therefore, NRIP is a novel structural component of NMJ complexes associated with AChR, rapsyn and ACTN2 ( Figure 1F).

NRIP is a novel AChRα binding protein
To confirm the direct binding between AChRα and NRIP in vitro, we first produced recombinant proteins His-MBP-NRIP and GST-AChRα from bacteria with purification using Ni-NTA and GST beads, respectively. Two recombinant proteins were then incubated together for direct interaction, followed by immunoprecipitation and Western blotting using antibodies specifically against His (NRIP) or GST (AChRα). The results showed a reciprocal binding of NRIP and AChRα in vitro (Figure 2A). To test the binding of NRIP and AChRα in cultured cells, HEK293T cells were transfected with EGFP-NRIP and Flag-AChRα. The immunoprecipitates with anti-EGFP (NRIP) or anti-Flag (AChRα) were analysed by Western blot, showing the appearance of AChRα and NRIP, respectively, indicating a reciprocal binding of NRIP and AChRα in the cellular system ( Figure 2B). We further investigated the endogenous binding of NRIP and AChRα in the cultured C2C12 cells. Protein lysates of C2C12 cells, which had differentiated for 5 days, were subjected to immunoprecipitation with anti-NRIP or anti-AChRα antibody, respectively. The following Western blot showed a reciprocal binding of NRIP and AChRα again, implying the binding of endogenous NRIP and AChRα in cultured myocytes ( Figure 2C). In sum, NRIP is a novel AChR binding protein.

NRIP stabilizes protein-protein interaction of AChR-rapsyn-ACTN2 complex
While NRIP is a structural component of the NMJ complex with direct interaction with AChR, we hypothesized that NRIP could stabilize the protein-protein interaction of the AChRrapsyn-ACTN2 complex. We compared the binding affinity of AChR and rapsyn in gastrocnemius muscles of wild-type and muscle-restricted NRIP conditional knockout mice using the B-BTX pull-down assay. The following Western blot for rapsyn showed lower binding affinity between AChR and rapsyn in NRIP knockout than wild-type mice ( Figure 3A; 0.638 vs. 1.079). Previously, we generated NRIP knockout C2C12 cells (using CRISPR Cas9) and named KO19. The B-BTX pull-down assay showed a lower binding affinity between AChR and rapsyn in KO19 than wild-type C2C12 cells ( Figure 3B; 0.622 vs. 0.993).
Similarly, we used muscle-restricted NRIP knockout mice and NRIP knockout C2C12 cells to test the binding affinity between rapsyn and ACTN2. Although the expression of rapsyn and ACTN2 was comparable in gastrocnemius muscles of wild-type and NRIP knockout mice, the binding affinity between rapsyn and ACTN2 in NRIP knockout mice was reduced than that in wild-type mice ( Figure 3C; 0.54 vs. 1.00). The binding affinity between rapsyn and ACTN2 is also lower in KO19 than wild-type C2C12 cells ( Figure 3D; 0.502 vs. 1.003). Taken together, NRIP strengthens binding affinity between AChR and rapsyn as well as between rapsyn and ACTN2 in both mouse muscles and C2C12 cells and acts as a scaffold protein stabilizing the AChR-rapsyn-ACTN2 complex.

Anti-NRIP autoantibodies in patients with MG
To investigate whether the anti-NRIP autoantibody exists in sera of MG patients, we first produced NRIP protein from HEK293T cells using various methods through transfection or viral vector infection. After 48 h, the NRIP expression was analysed using Western blot. Among different methods, NRIP-Flag produced by pAAV-NRIP-Flag plasmid transfection ( Figure S1, lane 6) had the best expression efficacy. Therefore, NRIP-Flag produced by this way was served as the antigen, and the GFP protein expressed via pAAV-GFP transfection by jetPRIME was served as the negative control in further Western blotting analysis. We used 50 μg of NRIP-Flag to be a standard amount after testing different doses of NRIP-Flag to determine the optimal quantity ( Figure 4A). Subsequently, the protein lysates from pAAV-NRIP-Flag transfected HEK293T cells were used as antigens, and the sera from patients with MG were used as primary antibodies in Western blot to detect the anti-NRIP autoantibody.
In 43 patients with MG (age, 58.4 ± 14.5 years; man, 19), 14 (32.6%) had ocular MG, and six (14.0%) ever required respiratory support. Thirty-three patients (76.7%) were seropositive for the anti-AChR antibody. Using Western blot, we found that NRIP autoantibodies were detectable in sera from six patients with MG ( Figures 4B and S2). To confirm the presence of anti-NRIP autoantibodies, different doses of NRIP-flag were tested in western blot. While all sera from 6 anti-NRIP seropositive patients did not react to GFP, they reacted to NRIP-Flag in a dose-dependent manner along with the increase of NRIP-Flag quantity ( Figure 4C). Therefore, we confirmed that anti-NRIP autoantibody exists in a small group of patients with MG (14.0%).

The existence of anti-NRIP autoantibody correlates with MG severity
We compared the clinical features of MG patients with (n = 6) and without (n = 37) anti-NRIP autoantibody (Table 1). There is no significant difference between groups regarding age, gender, disease duration, the existence of thymoma, ocular form or not, the requirement of respiratory support or hospitalization or the MGFACC. Notably, all six MG patients with anti-NRIP autoantibody also have anti-AChR autoantibody. Patients with MG and anti-NRIP autoantibody showed a trend to have unstable MG course (requiring titration of MG-specific medication within 1 year) compared with those without anti-NRIP autoantibodies, although the difference did not reach statistical significance (P = 0.077).

Figure 3 NRIP stabilizes the protein-protein interaction of AChR-rapsyn-ACTN2 complexes. (A)
Protein lysates were extracted from gastrocnemius muscles of wild-type (WT) or muscle-restricted NRIP conditional knockout (cKO) mice. The B-BTX pull-down assay followed by Western blot for rapsyn showed a lower binding affinity between AChR and rapsyn in cKO than WT mice. (B) Protein lysates were extracted from post-differentiative C2C12 cells without (WT) or with (KO19) NRIP knockout. The B-BTX pull-down assay showed a lower binding affinity between AChR and rapsyn in KO19 than WT C2C12 cells. (C) Although the expression of rapsyn and ACTN2 was comparable in gastrocnemius muscles of WT and NRIP cKO mice, the binding affinity between rapsyn and ACTN2 in NRIP cKO mice was reduced than that in mice. (D) The binding affinity between rapsyn and ACTN2 was lower in KO19 than WT C2C12 cells. The bar-grams show quantification of relative binding affinity from three separate experiments. The result from one of WT sample was set as 1. Data are mean ± standard error by Student's t-test. * P < 0.05 and *** P < 0.001.
We further correlated the existence of anti-AChR or anti-NRIP autoantibodies with MG severity, stratified by MGFACC class. Although the percentage of MG patients with anti-AChR autoantibody was not significantly associated with MG severity (P = 0.097) (Figure 5A), the severity of MG correlated with the percentage of patients having both anti-AChR and anti-NRIP autoantibodies (P = 0.011) ( Figure 5B). Besides, we also analysed the relationship between MG severity and the titre of anti-AChR or anti-NRIP autoantibodies. Though the titre of anti-AChR autoantibody was not significantly associated with MG severity (P = 0.123) ( Figure 5C), the severity of MG correlated with serum titre of anti-NRIP autoantibody (P = 0.032) ( Figure 5D). Taken together, the existence or higher titre of anti-NRIP autoantibody is associated with more severe disease severity of MG, implying the pathogenicity of anti-NRIP autoantibody.   Figure 6A. After 48 h of transfection, cell lysates were extracted and used as epitopes for Western blot. The sera of six MG patients with NRIP autoantibodies were used as primary antibodies. We found that most anti-NRIP autoantibodies recognized C-terminal truncated NRIP with or without deletion of WD6 and WD7 domains (Figures 6B and S3). The above results indicate that the epitope against NRIP autoantibody is mainly on the C-terminal but excluding WD6 and WD7 domains of NRIP, likely against the IQ domain of NRIP.

Most anti-NRIP autoantibodies belong to the IgG1 subclass
The immunoglobulin G is composed of four subclasses composed of four subclasses: IgG1, IgG2, IgG3 and IgG4. The different subclass of IgG causes immune responses by a different pathway. To understand the pathogenesis of NRIP autoantibody, we tried to determine the IgG subclass of anti-NRIP. After transfection of HEK293T cells with pAAV-NRIP-Flag, cell lysate was used as antigen in Western blot. The sera of MG patients with anti-NRIP autoantibody were used as primary antibody and different secondary antibodies for each human IgG subclass. We found that all six anti-NRIP seropositive patients with MG had anti-NRIP autoantibody belonging to IgG1, whereas one of them (MG35) also had IgG3 subclass anti-NRIP autoantibody (Figure 7).

Discussion
Previous studies have shown that muscular NRIP contributes to normal function and integrity of the neuromuscular system. [3][4][5] In this study, we specifically investigated the normal physiological and pathophysiological roles of NRIP in NMJ. We demonstrated that NRIP co-localizes with NMJ complex protein and interacts with AChR. NRIP knockout reduced AChR-rapsyn and rapsyn-ACTN2 binding affinity. The above findings indicate that NRIP is a novel NMJ component protein, which acts as a scaffold to stabilize the AChR-rapsyn-ACTN2 complex. In addition, we detected 14% of MG patients having anti-NRIP autoantibody. The presence and higher titre of anti-NRIP autoantibody correlated with a more severe type of MG. We also demonstrated that the immunogenic region of NRIP is likely on the IQ motif and anti-NRIP autoantibody mainly belongs to the IgG1 subclass. Therefore, the anti-NRIP antibody is a novel autoantibody in MG and plays a detrimental role in MG severity. NMJ is a chemical synapse between a motor nerve terminal and postsynaptic region on a muscle fibre. When the action potential arrives at the nerve terminal, the presynaptic terminal releases the neurotransmitter acetylcholine, which diffuses across the synaptic cleft and binds to AChR, leading to muscle contraction. 13 AChR binds to rapsyn, and rapsyn directly interacts with ACTN2 to induce AChR clustering and NMJ stability, 14,15 which ensure rapid, robust and reliable synaptic transmission. 16 In this study, we demonstrated that NRIP co-localizes with AChR, rapsyn and ACTN2 at NMJ of mouse muscle. We further confirmed that exogenous NRIP reciprocally interacts with exogenous α-subunit of AChR in both test tube and HEK293T cells. Endogenous NRIP also reciprocally interacts with AChR in C2C12 myocytes. Additionally, a previous study showed that NRIP directly interacts with ACTN2 at the Z-disc of cardiomyocytes. 17 These results indicate that NRIP is a structural component of AChRrapsyn-ACTN2 complex at NMJ (Figure 1F), having a direct interaction with α-subunit of AChR and probably ACTN2.
Deprivation of NRIP in skeletal muscles resulted in a small NMJ area with denervation of AChR. 5 The global or muscle-restricted NRIP knockout mice displayed impaired motor behaviours. 4,5 To investigate the function of NRIP at NMJ, we tried to knockout NRIP both in vivo and in vitro to test the stability of the AChR complex. The binding affinity between AChR and rapsyn as well as rapsyn and ACTN2 reduced in muscle-restricted NRIP knockout mice and NRIP knockout C2C12 cells. The above findings imply that NRIP acts as a scaffold to stabilize the AChR-rapsyn-ACTN2 complex. Without NRIP to hold the complex together, the efficacy of synaptic transmission and AChR clustering may reduce to cause abnormal NMJ architecture with impaired motor function, as noted in NRIP knockout mice. 5 MG is resulted from autoantibodies against the postsynaptic membrane of NMJ. The common autoantibodies include anti-AChR (75%-80%), anti-MuSK (1%-10%) and anti-Lrp4 (1%-5%). 10 In this study, we demonstrated that 14% of patients with MG having anti-NRIP autoantibody. The dose-dependent detecting signal using Western blot confirmed the existence of anti-NRIP autoantibody in sera of this small group of patients with MG ( Figure 4C). Notably, the presence of anti-NRIP autoantibody correlated with a more severe type of MG when anti-AChR autoantibody existed. In addition, the higher the titre of NRIP autoantibody, the more severe the MG severity. Patients with MG and having anti-NRIP autoantibodies also showed a non-significant trend to have unstable MG course. Therefore, anti-NRIP autoantibody did play a detrimental role in MG that patients with MG and having anti-NRIP autoantibody should be treated cautiously. Future extensive studies are mandatory to confirm the prevalence and clinical features of MG associated with anti-NRIP autoantibody.
Different autoantibodies of MG attack NMJ via different mechanisms. Anti-AChR autoantibody, mainly belonging to IgG1 and IgG3 subclasses, usually targets the main immunogenic region of AChR 18 and causes MG via activation of the classical complement cascade followed by membrane lysis, 19 triggering the endocytosis of AChR, and prevention of acetylcholine binding to AChR. 20 However, anti-MuSK antibody belongs to IgG4 subclass, which blocks MuSK activation, causing the reduced density of AChRs. 21 On the other hand, the pathogenetic roles of anti-Lrp4, anti-agrin and anti-ColQ autoantibodies are less understood. 8 In addition to the above autoantibodies targeting extracellular proteins, autoantibodies against intracellular antigens, such as anti-titin, antiryanodine receptor and anti-cortactin antibodies, are less likely pathogenetic for MG but markers for MG characteristics, such as disease severity, the presence of thymoma and myopathy. 8,22 To characterize the features of anti-NRIP autoantibody, we conducted an epitope mapping study, which showed that the immunogenic region of NRIP is likely on the IQ motif. We also demonstrated that the anti-NRIP autoantibody mainly belongs to the IgG1 subclass, which theoretically can activate the complement response. Because NRIP Figure 7 IgG subclass analysis for anti-NRIP autoantibody. HEK293T cells were transiently transfected with pAAV-NRIP-Flag for 48 h. Cell lysates were extracted and used as antigens for the detection of IgG subclass of anti-NRIP autoantibodies by Western blot. The sera of MG patients were used as primary antibodies. Different secondary antibodies for each human IgG subclass were used. The experiment with secondary antibodies only was served as negative control; the experiment with anti-human IgG secondary antibody, which can detect all the IgG subclass was served as the positive control. The Western blot shows that all the six anti-NRIP seropositive patients with MG had anti-NRIP autoantibody belonging to IgG1, whereas one of them (MG35) also had IgG3 subclass of anti-NRIP autoantibody.
did not have a transmembrane domain, 2,3 NRIP is likely an intracellular protein not exposed to extracellular space for the anti-NRIP antibody to attack. Considering that all MG patients with seropositive for anti-NRIP autoantibody also had concomitant anti-AChR antibody, we hypothesized that anti-AChR autoantibody first binds AChR with activation of the complement cascade and membrane destruction, leading to the exposure of NRIP. Anti-NRIP autoantibody subsequently attacks the IQ domain of NRIP and activates more complement responses. Therefore, patients with MG and having both anti-AChR and anti-NRIP autoantibodies showed worse outcomes than those without anti-NRIP autoantibody. In this study, we did not analyse other potential autoantibodies of MG such as anti-MuSK, anti-Lrp4, anti-agrin, anti-ColQ, anti-titin, anti-ryanodine receptor or anti-cortactin; therefore, the relationship between anti-NRIP to these autoantibodies is still unclear.
We also collected the sera from 19 healthy controls (mean age, 33.8 ± 12.4 years; man, 10) without ptosis, diplopia or limb weakness. Anti-NRIP autoantibody can be detected in six (31.6%) ( Figure S4A). Ten of 19 controls received the anti-NRIP test twice (mean interval of 5.0 ± 0.8 months), and the results of the two analyses were consistent ( Figure S4B). The epitope mapping of anti-NRIP autoantibodies from six anti-NRIP seropositive controls showed that most anti-NRIP autoantibodies targeted the C-terminal of NRIP but without WD6 and WD7 domains ( Figure S5), similar to that in patients with MG. These anti-NRIP autoantibodies also mainly belonged to the IgG1 subclass ( Figure S6). The titres of anti-NRIP autoantibody in six patients with MG and six control subjects were similar (0.78 ± 0.67 vs. 0.50 ± 0.47 mg/mL, P = 0.522). Taken together, anti-NRIP autoantibody appears in both patients with MG and control subjects. Without anti-AChR autoantibody, anti-NRIP autoantibody probably less likely disturbs NMJ function alone.
In summary, we demonstrated that NRIP is a novel AChR binding protein and NMJ structural component protein, which acts as a scaffold to stabilize the AChR-rapsyn-ACTN2 complex. This finding increases our understanding of the physiology of NMJ complex and expanses our knowledge of NRIP function in the neuromuscular system. Also, we showed that anti-NRIP autoantibody may be a novel autoantibody in MG, which increases the severity of MG with the coexistence of anti-AChR antibody.  Figure S2. Detection of anti-NRIP autoantibody in 43 patients with MG. The protein lysates of GFP and NRIP-Flag, produced by pAAV-GFP-Flag and pAAV-NRIP-Flag plasmid transfection, respectively, used as antigens in western blot; GFP was used as the negative control. The sera of 43 patients with MG, which were diluted at a ratio of 1:1000, were used as the primary antibodies. Sera showing positive signals with NRIP-flag but negative with GFP are anti-NRIP seropositive. Figure S3. The epitope mapping of anti-NRIP autoantibody from six patients with MG. The epitope against NRIP autoantibody is mainly on the C-terminal but excluding WD6 and WD7 domains of NRIP. Figure S4. Detection of anti-NRIP autoantibody in 19 control subjects. (A) Sera showing positive signals with NRIP-flag but negative with GFP are anti-NRIP seropositive. Six controls have anti-NRIP autoantibody (N1, N4, N7, N8, N11, and N16).
(2) Ten control subjects received the second test for the detection of anti-NRIP autoantibody with a mean interval of 5.0 ± 0.8 months. The results of the two analyses were consistent. Figure S5. The epitope mapping of anti-NRIP autoantibody from six healthy control subjects. The epitope against NRIP autoantibody is mainly on the C-terminal but excluding WD6 and WD7 domains of NRIP. Figure S6. IgG subclass analysis for anti-NRIP autoantibody from six healthy control subjects. The western blot shows that all the six anti-NRIP seropositive control subjects had anti-NRIP autoantibodies belonging to IgG1, while one of them (N11) also had IgG3 subclass of anti-NRIP autoantibody.