Investigation of the effect of dietary intake of omega‐3 polyunsaturated fatty acids on trauma‐induced white matter injury with quantitative diffusion MRI in mice

Abstract Previous studies suggest that long‐term supplementation and dietary intake of omega‐3 polyunsaturated fatty acids (PUFAs) may have neuroprotective effects following brain injury. The objective of this study was to investigate potential neuroprotective effects of omega‐3 PUFAs on white matter following closed‐head trauma. The closed‐head injury model of engineered rotational acceleration (CHIMERA) produces a reproducible injury in the optic tract and brachium of the superior colliculus in mice. Damage is detectable using diffusion tensor imaging (DTI) metrics, particularly fractional anisotropy (FA), with sensitivity comparable to histology. We acquired in vivo (n = 38) and ex vivo (n = 41) DTI data in mice divided into sham and CHIMERA groups with two dietary groups: one deficient in omega‐3 PUFAs and one adequate in omega‐3 PUFAs. We examined injury effects (reduction in FA) and neuroprotection (FA reduction modulated by diet) in the optic tract and brachium. We verified that diet did not affect FA in sham animals. In injured animals, we found significantly reduced FA in the optic tract and brachium (~10% reduction, p < 0.001), and Bayes factor analysis showed strong evidence to reject the null hypothesis. However, Bayes factor analysis showed substantial evidence to accept the null hypothesis of no diet‐related FA differences in injured animals in the in vivo and ex vivo samples. Our results indicate no neuroprotective effect from adequate dietary omega‐3 PUFA intake on white matter damage following traumatic brain injury. Since damage from CHIMERA mainly affects white matter, our results do not necessarily contradict previous findings showing omega‐3 PUFA‐mediated neuroprotection in gray matter.

While several studies suggest that omega-3 PUFAs could be protective in gray matter, evidence of neuroprotection in white matter is lacking. The investigation of potential neuroprotective effects of omega-3 PUFA in white matter is important for human TBI, since human TBI often includes white matter damage known as diffuse axonal injury (DAI; Adams et al., 1989;Johnson, Stewart, & Smith, 2013). Few results from rodent TBI models suggest that omega-3 PUFAs may reduce axonal damage by different mechanisms, including lowering the levels of beta amyloid precursor protein and nonphosphorylated neurofilament protein Mills, Bailes, Sedney, Hutchins, & Sears, 2010;Mills, Hadley, & Bailes, 2011), preserving myelin by maintaining levels of myelin basic protein, and protecting oligodendrocytes from excitotoxicity damage (Pu et al., 2013(Pu et al., , 2017. However, these effects have been observed in the Marmarou and controlled cortical impact (CCI) injury models that do not biomechanically resemble human TBI and do not produce comparable white matter damage (Namjoshi et al., 2013), making it difficult to extend protective effects of omega-3 PUFAs to human injury.
The closed-head injury model of engineered rotational acceleration (CHIMERA) was developed as a reproducible model that mimics the white matter damage observed in human TBI (Namjoshi et al., 2014). Histological assessment identified the optic tract and brachium of the superior colliculus as two regions severely affected by CHIMERA. Silver-staining revealed prominent axonal injury and glial fibrilary acidic protein (GFAP) and ionized calciumbinding adapter molecule 1 (Iba-1) immunohistochemistry indicated increased numbers of glia in these regions (Haber et al., 2017;Namjoshi et al., 2014). Ex vivo diffusion tensor imaging (DTI) showed that fractional anisotropy (FA) was significantly reduced in the optic tract and brachium of the superior colliculus in the injured animals that corresponded with axonal degeneration and gliosis observed in the histological analysis (Haber et al., 2017). This previous study found that diffusivity measures (Trace, AD, RD) were not as sensitive as FA to the damage, and validated the use of FA as a biomarker with specificity and sensitivity comparable to that of histology to identify damage from CHIMERA.
The main objective of this study is to investigate a potential neuroprotective effect of a diet adequate in omega-3 PUFAs on white matter damage from closed-head TBI. CHIMERA is an ideal model because it is reproducible, damage is specifically in white matter, and we have data on the specificity and sensitivity of FA as a biomarker for damage from CHIMERA in the optic tract and brachium of the superior colliculus. In this study, we examine FA values from ex vivo DTI to replicate injury effects observed previously (Haber et al., 2017), then investigate neuroprotective effects of the adequate diet.
Because our ultimate goal is to apply these techniques to studying human TBI, we performed an identical analysis in vivo to determine if effects observed ex vivo can be detected noninvasively in vivo.

K E Y W O R D S
closed-head injury, diffusion tensor imaging, mouse, MRI, omega-3 fatty acids, traumatic brain injury Significance Omega-3 fatty acid supplementation has shown positive, anti-inflammatory effects following stroke and traumatic brain injury (TBI). Positive effects on these high incidence and prevalence conditions have contributed to the popularity of omega-3 fatty acids with a $13 billion global market. Recent research on heart disease and cancer, however, suggest that omega-3 fatty acids may not provide the health benefits that were initially expected. In the brain, there is still an exclusive predominance of positive results.
To our knowledge, our study is the first that casts doubt that omega-3 fatty acid supplementation may have neuroprotective effects on tissue damage from TBI.

| Experimental subjects
The study included adult C57BL/6 male mice (Charles River Laboratories, Wilmington, MA) that were fed either a diet with adequate omega-3 PUFA levels or deficient omega-3 PUFA levels and received either repeated CHIMERA or sham injury. The diets were manipulated such that the omega-6 PUFA composition was similar in the adequate and deficient diets (linoleic acid = 18.2% in the deficient diet vs. 17.2% in the adequate diet) and differed substantially in omega-3 PUFA composition (alpha-linolenic acid = 0.04% in the deficient diet vs. 3.8% in the adequate diet). Additional nonfatty acid ingredients were identical between the two diets. The omega-3 PUFA-deficient diet began during gestation resulted in a 35% depletion of omega-3 PUFAs (Desai et al., 2016). These diets were maintained following CHIMERA for the duration of the study period. The normal 12-hr light period was maintained in the animal housing facility, and mice had free access to food and water.
The in vivo sample included 38 mice with sham-adequate n = 9, sham deficient n = 12, CHIMERA-adequate n = 9, CHIMERA deficient n = 8. The ex vivo sample included 41 mice with sham-adequate n = 9, sham deficient n = 14, CHIMERA-adequate n = 11, CHIMERA deficient n = 7. Thirty-three animals were in common between the ex vivo sample and the in vivo sample.

| Sample size estimation
CHIMERA produces a highly reproducible injury with tissue damage that is observable by eye with evident silver staining in the optic tract and brachium of the superior colliculus at the individual level (Haber et al., 2017;Namjoshi et al., 2014). A previous ex vivo DTI study (Haber et al., 2017) showed a measurable and significant effect of CHIMERA in these same regions in a relatively small sample (injured animal = 5, sham animal = 5). Based on the results of this previous ex vivo DTI experiment, we set our minimum sample size at N = 5 and added additional animals to account for the possible introduction of variability from noise or other factors in the in vivo scans as well as to account for possible animal attrition. We did not perform a formal power analysis a priori since this would require a full characterization of experimental variability and population variability, which was not possible in this case since the previous ex vivo DTI study was performed on a different scanner with a different DTI acquisition scheme.

| CHIMERA procedure
The mice underwent repeated CHIMERA or sham injury at 3-4 months old (Namjoshi et al., 2014). Prior to the procedure, the mice were anesthetized with 5% isoflurane for 2 min in oxygen (1L/min) and positioned supine on the holding platform of the CHIMERA apparatus angled at ~32 degrees to place the top of the head flat over a hole in the head plate. The mouse head was aligned so that the impact was limited to the dorsal cortical region in the 5 mm area surrounding bregma. The input kinetic energy (Ek) was calculated using the formula Ek = 1/2 mv 2 (v = velocity (m/sec), m = mass of piston). In this study, a 0.55J kinetic energy was delivered to the mouse head using a 50-g piston. Anesthesia was discontinued once the impact occurred. The injured mice received three impacts 24 hr apart. The sham mice were given all the procedures except for the impact. After each impact, the mice were monitored and were continuously observed until recovery.
The injured mice did not exhibit fractures, hematomas, apnea, or death following the impact.

| Sample preparation for ex vivo imaging
Following the in vivo MRI acquisition, each mouse was administered an i.p. overdose of Euthasol (50 mg/kg). Once the mouse no longer responded to a toe pinch, it was transcardially perfused with 250 ml of ice-cold phosphate-buffered saline (PBS, pH 7.4) containing 2% heparin (Sigma-Aldrich) followed by 250 ml of ice-cold 4% paraformaldehyde solution in PBS (Santa Cruz Biotechnology). The skull was then removed from the body and was postfixed in the 4% paraformaldehyde solution for 24 hr and then transferred to a solution containing 0.01% sodium azide in PBS for storage. After at least 1 week of rehydration in this solution, the soft tissue and mandibles were removed from the skulls, and the remaining cranium was immersed in Fluorinert (FC-3283, 3M, St. Paul, MN, United States) in a 5-ml plastic syringe for imaging.

| Image acquisition and processing
Imaging was performed on the mice in the chronic period (greater than 3 months) following injury with repeated CHIMERA or sham  Irfanoglu, Nayak, Jenkins, & Pierpaoli, 2017;Pierpaoli et al., 2010).
For the in vivo and ex vivo data, the diffusion tensor was fit using the DIFFCALC v2.5 module of TORTOISE and was used to generate FA (Basser & Pierpaoli, 1996) and directionally encoded color (DEC) maps (Pajevic & Pierpaoli, 1999).
Separate study-specific DTI templates were created for in vivo and ex vivo images using diffeomorphic registration for tensor accurate alignment of anatomical structures (DR-TAMAS), and the resulting deformation fields were used to put the native scalar maps into the study-specific DTI template space (Irfanoglu et al., 2016).

| Optic tract and brachium region of interest (ROI)
A specific ROI encompassing the optic tract and brachium of the superior colliculus was used for this analysis. This ROI was defined because these two regions had been shown to be the most severely injured in CHIMERA in a previous ex vivo study (Haber et al., 2017).
This ROI was manually drawn on each study-specific template (in vivo and ex vivo templates) using anatomical guidance about the tract location from DEC maps and the Allen mouse brain atlas (Lein et al., 2007).

| Statistical analysis
The central focus of this study was to investigate a potential neuroprotective effect of a diet adequate in omega-3 PUFAs on white matter damage after a closed-head brain injury. As a measure of white matter damage, we computed median FA value in the optic tract and brachium ROI for each animal in both the ex vivo and in vivo samples.
We included data from all acquired scans in our analyses. The analyses were performed in R using ANTsR (v0.7.1) for reading and writing images, Tidyverse (v1.2.1) packages for organizing and plotting data, and base R statistics (v3.4.2) and BayesFactor (0.9.12-4.2) for statistical analyses (Avants, 2017;Morey & Rouder, 2018;R Core Team, 2017;Wickham, 2017). Identical analyses were performed in the ex vivo and in vivo samples. For one-tailed tests, we used α = 0.025, while for two-tailed tests α = 0.05. We performed a multiple comparisons correction for all p values obtained in the study using a false discovery rate (FDR) correction.
To assess a potential neuroprotective effect of omega-3 PUFAs, we first needed to rule out the possibility that the adequate diet itself may affect FA values. Since diet had never been investigated on its own and we had no prior evidence for how diet may affect FA values, this component of the study was exploratory. We performed our analysis in the sham animals. To examine the diet effect, we used a two-tailed Welch t-test to investigate differences in FA between the adequate and deficient diets in the sham group, that is, differences in group means (adequate vs. deficient) of the distributions of median FA measured for the individual animals (see Figure 1). A statistically significant result would indicate a non-negligible diet effect that would need to be accounted for when interpreting the results from the subsequent analyses. We also computed Hedge's g (difference of group means/pooled standard deviation weighted by sample size) using adequate sham-deficient sham for the numerator (Hedges, 1981).
Next, we needed to confirm that we are able to detect the injury effect and replicate the findings from Haber et al. (2017).
Based on this previous work, we expect that the median FA values will be lower in injured animals compared to the sham animals.
To test this hypothesis, we performed one-tailed Welch t-tests separately within the adequate and deficient diet groups to test if median FA values in the injured group were lower than those in the sham group, and computed Hedge's g for each comparison (CHIMERA-sham/pooled standard deviation weighted by sample size). We performed the tests separately in each diet group assessing differences in group means (injured vs. sham) of median FA of the individual animals to take into account any potential neuroprotective effect of the diet that might affect the severity of the injury.
We focused on the injured animals to examine the potential neuroprotective effect of omega-3 PUFAs. We formed our hypothesis to test for a neuroprotective effect of the adequate diet based on the previous results from Haber et al. (2017) that showed that the injury resulted in reduced FA. If the adequate diet offers the white matter neuroprotection against damage, the injured animals on the adequate diet should have FA values that are more similar to those of the unaffected sham animals. Therefore, we expect that the injured animals on the adequate diet should have higher median FA than the injured animals on the deficient diet. We applied a one-tailed Welch t-test to test if the CHIMERA-adequate group had higher median FA than the CHIMERA-deficient group, based on the group means of median FA of the individual animals of those groups. Significantly higher median FA in the CHIMERAadequate group would indicate a neuroprotective effect of the diet on white matter damage resulting from CHIMERA. We also computed Hedge's g for this comparison (adequate CHIMERAdeficient CHIMERA/pooled standard deviation weighted by sample size).
The previous tests we performed fall within the framework of null hypothesis significance testing (NHST). In NHST, a null hypothesis, or no effect, is proposed and the data are then tested to assess if it conforms to the null hypothesis at a given significance level, or p value (Pernet, 2016). The p value, however, only expresses the probability of obtaining a result as extreme as the one observed assuming that the null hypothesis is true, and does not express the magnitude of the effect nor the strength of evidence for accepting or rejecting the null hypothesis (Krzywinski & Altman, 2013).
To assess the level of confidence we have in accepting or rejecting the null hypothesis, we can compute Bayesian inference to examine the weight of evidence for the null hypothesis compared to an alternate hypothesis of a nonzero effect (Faulkenberry, 2018). To compare the relative probability of two different hypothesis, such as the null hypothesis (H 0 ) for no effect and an alternative hypothesis (H A ) for a nonzero effect, Bayes' theorem can be applied in the following manner: F I G U R E 1 Violin plots showing the distributions of the median FA values in the optic tract and brachium ROI by diet and injury group for ex vivo (a) and in vivo (b), with the mean (solid diamond) ± standard deviation (bars), and individual data points (transparent dots). For the ex vivo sample, we did not detect a significant diet effect in the sham group, and the Bayes factor indicated anecdotal evidence to accept the null hypothesis (BF = 0.419). Both diet groups showed significantly lower FA in the CHIMERA group compared to the sham group, and the Bayes factors showed strong evidence to reject the null hypothesis (deficient BF = 48.7 × 10 4 , adequate BF = 79.8). The CHIMERA deficient and CHIMERAadequate groups were not significantly different and the Bayes factor indicated substantial evidence to accept the null hypothesis (BF = 0.241). There was large overlap between the two groups. The in vivo sample showed similar results as the ex vivo sample with no significant diet effect (BF = 0.715), significant injury effects in both diet groups (deficient BF = 1.90 × 10 5 , adequate BF = 59.0), and no significant difference between the CHIMERA deficient and CHIMERA-adequate groups (BF = 0.247), indicating no neuroprotective effect of diet For our study, we assume the same prior probability (1:1) for both hypotheses since we believe that the null and alternative hypotheses are equally likely. Therefore, any change in the posterior probability ratio compared to the prior probability (i.e., any deviation from 1:1) would result from a change in the likelihood of one hypothesis compared to the other. This change in the ratio of likelihoods is the Bayes factor, and it provides a means of assessing the weight of evidence for either hypothesis (Kass & Raftery, 1995;Jeffreys, 1961

| RE SULTS 3.1 | Diet effect
We found no detectable effect of diet in the optic tract and brachium ROI. In the ex vivo sample, FA values in the sham-adequate group were not significantly higher than those in the sham-deficient group and showed a negligible effect size (Table 2, Figure 1a), while the Bayes factor for this comparison suggested anecdotal evidence for accepting the null hypothesis. A comparison of the distributions using a K-S test indicated that there was no significant difference between voxel-wise median FA values in the ROI (Table 3) Figures 1b and 3), and the Bayes factor showed anecdotal evidence to accept the null hypothesis ( Table 2). The K-S test also did not show a significant difference between the diets (Table 3), and the distributions of the sham-adequate and sham-deficient groups were similar (Figure 2b).

| Injury effect
We replicated the previous results from Haber et al. (2017) (Table 3, Figures 1b and 3), and the Bayes factors indicated strong evidence to reject the null hypothesis (Table 4). The K-S also detected a significant injury effect in both diet groups (Table 3), with the distributions showing nonoverlapping distributions between the sham and CHIMERA groups (Figure 2b).

| Neuroprotective effect
We did not detect a neuroprotective effect of the adequate diet following the injury in the ex vivo sample. There was no significant difference in FA between the adequate and deficient CHIMERA groups with the effect size showing a medium effect in the opposite direction that we hypothesized in our one-sided t-test (deficient CHIMERA FA > adequate CHIMERA FA), and the Bayes factor showing substantial evidence to accept the null hypothesis. The K-S test also showed no difference between the two groups (Table 3), and the distributions of the two groups were largely overlapping (Figure 2a).
We did not detect a neuroprotective effect in the in vivo sample.
The in vivo data also showed no significant difference in FA between the CHIMERA-adequate and deficient groups with a medium effect size in the opposite direction of the t-test, and the Bayes factor indicated substantial evidence to accept the null hypothesis (Table 5, Figure 1b). The K-S test showed similar results (Table 3), and the distribution of voxel-wise group median FA values for the CHIMERAadequate and deficient groups mostly overlapped (Figure 2b).

| D ISCUSS I ON
Our goal for this study was to investigate the effect of dietary intake of omega-3 PUFAs in modulating the degree of white matter  injury in mild TBI. We replicated previous results and showed a significant effect of the injury on FA in the optic tract and brachium of the superior colliculus, and the Bayes factor analysis indicated very strong and decisive evidence for higher FA values in sham animals compared to injured animals. Even though we detected the injury effect, we found no evidence of a neuroprotective effect of the diet adequate in omega-3 PUFAs. The injured animals did not show a significant difference in FA between the adequate and deficient diet groups, and the Bayes factor analysis indicated substantial evidence to accept the null hypothesis and suggests that our results are not random or due to a sampling error. Moreover, the voxel-wise FA distributions (Figure 2) of the two injured groups overlap, showing that the adequate diet had no effect on FA values following CHIMERA.
Additionally, we were able to detect similar effects using both ex vivo and in vivo data, demonstrating that in vivo DTI is also sensitive to detecting the white matter damage resulting from CHIMERA.

| Injury effect
Previous histological analyses indicated that two of the most damaged structures following CHIMERA were the optic tract and brachium of the superior colliculus (Haber et al., 2017;Namjoshi et al., 2014). These regions showed abundant silver staining suggesting disruption to the white matter. Staining with a pan-neurofilament antibody revealed that this damage was in part a result of damaged axons, and IBA-1 and GFAP staining showed increased glial activity after injury (Haber et al., 2017). image showed a reduction in FA for the CHIMERA group in the optic tract and brachium of the superior colliculus, the same regions shown by histology to be severely damaged. This is in line with previous work that has shown that reduced anisotropy can be linked with demyelination and axonal generation (Budde, Janes, Gold, Turtzo, & Frank, 2011), as well as increased number of glial cells (Saadani-Makki et al., 2009).
In the current study, we were able to replicate the injury effect in FA, and clearly detected reduced FA in the optic tract and brachium of the superior colliculus in injured animals for both diet groups (Figure 4).
We also observed similar results in vivo and showed that white matter injury in this model can be detected noninvasively by MRI. Remarkably, we still observed such a strong effect in both the ex vivo and in vivo data at a chronic time point of more than 3 months after injury, indicating that CHIMERA results in persistent disruption to the white matter.

| Neuroprotective effect
While previous research has shown evidence for a neuroprotective of omega-3 PUFAs on tissue damage following brain injury, our findings suggest that this effect may not be generalizable to all injury types. Much of the previous evidence for neuroprotection comes from work on ischemic models of brain injury. Ischemia results from restriction of cerebral blood flow that can deprive the brain tissue of oxygen and glucose, and can cause an accumulation of toxic substances that ultimately leads to cell death (Bramlett & Dietrich, 2004). Treatment with omega-3 PUFAs both before and after ischemia results in reduced infarct volume and tissue damage, lower numbers of pro-inflammatory glia, and a reduction in histological markers of cell death both at and near the infarct site (Belayev et al., 2011;Belayev, Khoutorova, Atkins, & Bazan, 2009;Cai et al., 2018;Fernandes et al., 2008;Hong, Belayev, Khoutorova, Obenaus, & Bazan, 2014;Jiang et al., 2016;Lalancette-Hébert et al., 2011;Okada et al., 1996;Zhang et al., 2014Zhang et al., , 2010.
TBI involves mechanical stresses on the tissue from an impact or acceleration and deceleration of the head. These mechanical effects can cause contusions and breakage of blood vessels that alter the cerebral blood flow to the brain tissue and result in effects similar to those observed in ischemia, but the mechanical stresses cause additional shearing and stretching of axons that lead to widespread white matter damage (Bramlett & Dietrich, 2004). A few studies have shown that omega-3 PUFAs can decrease white matter damage by protecting myelin (Pu et al., 2013(Pu et al., , 2017, and can reduce the markers of axonal damage such as APP and SMI-32 Mills et al., 2010Mills et al., , 2011, but these studies were performed using injury models that are not biomechanically compatible with the type of injury most often seen in human TBI.
The current study applied CHIMERA as an injury model since it closely reproduces the type of white matter damage observed in human TBI and includes head rotation. In mice, CHIMERA reliably produces axonal damage and induces inflammatory effects in the optic tract and brachium of the superior colliculus (Haber et al., 2017;Namjoshi et al., 2014). This pattern of damage differs from the types investigated in the previous studies, which included a focal lesion (Pu et al., 2013(Pu et al., , 2017, and strong damage to the corticospinal tract and medial lemniscus Mills et al., 2010Mills et al., , 2011.
While our results do not refute those observed in these other injury models, they do bring into question the efficacy of omega-3 PUFAs to provide neuroprotection against tissue damage following all types of injury, since it is possible that the biomechanical differences between CHIMERA and these other models result in different pathophysiological effects, or that damage to cerebral white matter is not as accessible or responsive to omega-3 PUFAs as tissue near a lesion or in the brainstem. Future work might examine differences in omega-3 PUFA neuroprotection between models more closely to determine how the omega-3 PUFAs might best be translated to the study of human TBI.
Additionally, the findings of the current study may depart from those in previous studies that show a robust neuroprotective effect of omega-3 PUFAs because we investigated neuroprotective effects of the adequate diet at a chronic time point of more than 3 months postinjury. Most of the previous studies limited the postinjury time period to 7-35 days (Trépanier et al., 2016), and only two studies examined neuroprotective effects at 8 weeks (Cai et al., 2018;Fernandes et al., 2008). Investigating the efficacy of a treatment at a more chronic time point is especially important for translational studies of TBI, since even mild TBI is capable of producing long-lasting effects in the human brain that can worsen with time (Corrigan & Hammond, 2013).
For any treatment, or in this case, diet, to be considered effective, the beneficial effects should be detectable at a chronic time point in regions with remaining tissue damage. In this case, we did not detect any beneficial effects from the diet and found a paradoxical effect of reduced FA in the injured adequate diet group, suggesting that the adequate diet did not provide neuroprotection from injury. The possibility remains that the dietary effects may have been more easily observable in the acute period and may have resulted in a faster recovery in animals with the adequate diet. However, even if the diet was to confer such benefits in the acute period after injury, the lack of long-term effects indicates that this type of dietary intervention may not be helpful for protecting brain tissue following a type of injury known to have so many chronic effects. To characterize the potential neuroprotective effect across the entire recovery period, future work might employ a longitudinal study design that uses multiple time points within the same animal to distinguish between neuroprotection in the acute and chronic phases postinjury and also reduce variability to better detect subtle changes due to the diet.

| Ex vivo and in vivo imaging results
In vivo MRI can be performed noninvasively and is an essential tool for translating findings from animal models into research and applications for human patients. In vivo imaging, however, can be limited by reduced SNR and increased image artifacts compared to ex vivo imaging (Sadeghi et al., 2015;Walker et al., 2011). While many of these issues can be corrected in processing, in vivo imaging may still suffer from lower resolution due to a shorter allowable scan time that may result in partial volume effects that reduce the ability to detect more subtle microstructural differences. It is reassuring to see that in vivo imaging is as sensitive to detecting white matter injury resulting from CHIMERA as ex vivo imaging.
The in vivo diffusion MRI results from the current study replicate the results that have been found using ex vivo data. We have shown that in vivo diffusion MRI is capable of detecting white matter damage and can be useful in detecting such effects resulting from TBI.
Our results from in vivo MRI are also particularly encouraging for the study of white matter damage in human patients suffering from persistent effects of TBI since we were still able to detect these effects in the chronic period following the injury.

| CON CLUS IONS
In summary, in the current study, we applied both ex vivo and in vivo quantitative diffusion MRI to investigate a potential neuroprotective effect of omega-3 PUFAs on mild TBI. We replicated the effects of CHIMERA that had been previously observed with histology ex vivo DTI and histology with both sets of data. While we clearly replicated ex vivo and in vivo the detection of significant injury effects for FA in the optic tract and brachium of the superior colliculus that were shown in previous studies, our results also indicate that omega-3 PUFAs do not offer a neuroprotective effect on white matter injury resulting from mild TBI in the CHIMERA model. Because we focused on the chronic phase greater than 3 months after the injury, further investigation is necessary to investigate potential neuroprotective effects of omega-3 PUFAs longitudinally to fully characterize effects throughout the acute and chronic phases postinjury and eliminate intersubject variability to detect more subtle microstructural differences. Future work might also compare neuroprotective effects of omega-3 PUFAs in different injury models to assess their applicability to human TBI.

DECL AR ATION OF TR ANS PAREN C Y
The authors, reviewers and editors affirm that in accordance to the policies set by the Journal of Neuroscience Research, this manuscript presents an accurate and transparent account of the study being reported and that all critical details describing the methods and results are present.

ACK N OWLED G M ENTS
The Inc.

CO N FLI C T O F I NTE R E S T
No potential conflict of interest was reported by the authors.

PEER R E V I E W
The peer review history for this article is available at https://publo ns.com/publo n/10.1002/jnr.24705.

DATA AVA I L A B I L I T Y S TAT E M E N T
The data that support the findings of this study are available from the corresponding author upon reasonable request.