BRCA1/BRCA2 mutations in Japanese women with ductal carcinoma in situ

Abstract Background Ductal carcinoma in situ (DCIS) is considered a component of the clinical spectrum of breast cancer even in those with BRCA1/2 mutation. The aim of this study was to report the feature of DCIS raised in Japanese women with BRCA1/2 mutations. Methods A total of 325 Japanese women with breast cancer (BC) (with or without invasive cancer) were referred for genetic counseling and underwent genetic testing for mutations in the BRCA1 and BRCA2 genes in Showa University Hospital between December 2011 and August 2016. And 49 of them who were pathologically diagnosed as DCIS were included in this study. Logistic regression models were fit to determine the associations between potential predictive factors and BRCA status. A Cox proportional hazards model is used to predictive value of parameters for Ipsilateral breast tumor recurrence (IBTR) and contralateral breast tumor recurrence (CBTR). Results (a) Of 325 patients (with or without invasive cancer), 19.1% (62/325) tested positive for BRCA1/BRCA2 mutations. And 18.4% (9/49) was positive for BRCA1/BRCA2 mutations in DCIS, compared with 19.2% (53/276) in IDC (p = 1.000). Among BRCA mutations, 14.5% (9/62) had DCIS compared with nonmutations (15.2%, 40/263). Incidence of DCIS was 3.0% (1/33) of BRCA1 mutations and 27.5% (8/29) of BRCA2 mutation (p = 0.009). (b) Median age of diagnosis in BRCA mutation carriers was 39 years, compared with 46 years in noncarriers. Age, Family history (FH) of BC, FH of first or second BC and total number of relatives with BC diagnosis (DX) has significant difference between BRCA mutation carriers and noncarriers in univariate analysis. In a multivariate logistic model, total relatives with BC DX ≥ 2 (odds ratio [OR], 5.128; 95% confidence interval [CI], 1.266–20.763; p = 0.022), age at diagnosis ≤35 years (OR 0.149, 95% CI 0.023–0.954, p = 0.045) and ER+/HER2+ status (OR 5.034, 95% CI 1.092–23.210, p = 0.038) remained as independent significant predictors for BRCA mutation. Ki67 index (cut off by 14% or 30%) did not differ between BRCA mutation carriers and noncarriers (p = 0.459 and p = 0.651). (c) There was a significant difference in ER‐positive tumors among BRCA2 carriers and noncarriers (p = 0.042). Subgroup analysis showed BRCA2 carriers tend to be of higher grade (Grade 2 and 3), more frequently ER+/PR+ (p = 0.041) and lower proliferation (Ki67 index) than noncarriers, whereas differences in nuclear grade and ki67 index were not found significantly in our study. (d) BRCA mutation was not associated with an increased risk of IBTR and CBTR. Conclusion DCIS is equally as prevalent in patients who were BRCA mutation carriers as in high familial‐risk women who were noncarriers, but occurs at earlier age. BRCA2 carriers have higher incidence in DCIS than that of BRCA1 carriers, and tend to be higher grade and more frequently ER positive and lower proliferation. Total relatives with BC DX ≥2, age at diagnosis ≤35 years and ER+/HER2+ might be independent predictors for BRCA mutation in Japanese women with DCIS and patients of these risk factors should be recommended to receive genetic counseling and BRCA testing.

tween December 2011 and August 2016. And 49 of them who were pathologically diagnosed as DCIS were included in this study. Logistic regression models were fit to determine the associations between potential predictive factors and BRCA status.

| INTRODUCTION
Since 1996, breast cancer has become the most frequent malignancy in women in Japan and estimates of new cases in 2016 were 90,000 according to National Cancer Center of Japan. Widespread adoption of screening increases breast cancer incidence in a given population and changes the characteristics of cancers detected, with increased incidence of lower-risk cancers, premalignant lesions, and ductal carcinoma in situ (DCIS). Although DCIS is not immediately lifethreatening, it is associated with an increased risk of invasive breast cancer (IBC). Pathologists agree that DCIS is a preneoplastic lesion, and shares certain features with IBC such as genetic signature and epidemiologic risk factors (Fujii, Szumel, Marsh, Zhou, & Gabrielson, 1996;Hwang et al., 2004;O'Connell et al., 1998).
The identification of deleterious mutations in the breast cancer susceptibility genes BRCA1 and BRCA2 has important implications for mutation carriers in general, because they are the principal cause of Hereditary Breast and/or Ovarian Cancer Syndrome (Miki et al., 1994;Wooster et al., 1995). Of all women with breast cancer, 5% to 10% may have a germline mutation of the genes BRCA1 and BRCA2 (Blackwood & Weber, 1998). The estimated lifetime risk of developing breast cancer for women with BRCA1 and BRCA2 mutations is 40% to 85%. Mutation carriers with a history of breast cancer have an increased risk of contralateral disease that may be as high as 5% per year (Frank et al., 1998). Mutations in either the BRCA1 or the BRCA2 gene also confer an increased risk of ovarian cancer (Easton et al., 1999;Ford, Easton, Bishop, Narod, & Goldgar, 1994) or other primary cancers (Easton et al., 1999;Ford et al., 1994). When BRCA1/BRCA2 mutation carriers were diagnosed before age 40 years, the risk of a contralateral breast cancer (CBC) reached nearly 50% in the ensuing 25 years (Garber & Golshan, 2009;Graeser et al., 2009).
The association of BRCA mutations with IBC is well established. DCIS is now considered a component of the BRCA mutation clinical spectrum (Bayraktar et al., 2012;Hall, Reid, & Wenstrup, 2010;Hwang et al., 2007;Smith et al., 2007). A few retrospective studies have examined the prevalence of BRCA1/BRCA2 mutations in women diagnosed with DCIS and have reported mutation rates ranging between 3.3% (Claus, Petruzella, Matloff, & Carter, 2005) and 13% (Frank et al., 2002;Hall, Reid, & Wenstrup, 2010). Those studies support the concept that women with DCIS, like their counterparts with IBC, warrant genetic risk assessment and testing on the basis of high-risk variables. Notably, the knowledge of a BRCA1/BRCA2 mutation is likely to significantly change the assessment of a DCIS patient's risks for future cancers and the cancer prevention/ risk reduction recommendations that would be considered. Subsequent studies have led to the suggestion that the preinvasive phase may be shortened or even absent in hereditary breast cancers, particularly those associated with BRCA1 mutations (Jacquemler, Eisinger, Guinebretiere, Stoppa-Lyonnet, & Sobol, 1996). But patients in studies above are almost from US and other Western countries, while only a few reports have been published concerning the prevalence of BRCA1/BRCA2 mutations with DCIS among Japanese people (Ikeda et al., 2001;Kawahara et al., 2004;Noguchi et al., 1999;Sekine et al., 2001). noncarriers (p = 0.459 and p = 0.651). (c) There was a significant difference in ERpositive tumors among BRCA2 carriers and noncarriers (p = 0.042). Subgroup analysis showed BRCA2 carriers tend to be of higher grade (Grade 2 and 3), more frequently ER+/PR+ (p = 0.041) and lower proliferation (Ki67 index) than noncarriers, whereas differences in nuclear grade and ki67 index were not found significantly in our study.
(d) BRCA mutation was not associated with an increased risk of IBTR and CBTR. Conclusion: DCIS is equally as prevalent in patients who were BRCA mutation carriers as in high familial-risk women who were noncarriers, but occurs at earlier age.
BRCA2 carriers have higher incidence in DCIS than that of BRCA1 carriers, and tend to be higher grade and more frequently ER positive and lower proliferation. Total relatives with BC DX ≥2, age at diagnosis ≤35 years and ER+/HER2+ might be independent predictors for BRCA mutation in Japanese women with DCIS and patients of these risk factors should be recommended to receive genetic counseling and BRCA testing.

K E Y W O R D S
BRCA 1/2 mutations, breast cancer, ductal carcinoma in situ, family history | 3 of 12 LIU et aL.
The purpose of our study is to further characterize the association between DCIS and BRCA mutations in Japanese women and to provide the genetic basis for directing the treatment and predicting prognosis for DCIS women with BRCA1/BRCA2 mutation. We also hope to find special independent predictors for BRCA mutation in DCIS and could recommend genetic counseling and testing for Japanese patients with DCIS of high risk of BRCA mutation.

| Patients
A total of 325 Japanese women with BC (with or without invasive cancer) were referred for genetic counseling and underwent genetic testing for mutations in the BRCA1 and BRCA2 genes in Showa University Hospital from December 2011 to August 2016. Each participant was either self-or physician-referred to genetic counseling. And 49 of them who were pathologically diagnosed as DCIS were included in this study. Any patients with IBC, identified either at time of biopsy or after tumor removal surgery, were excluded. All pathologic specimens were reviewed by a dedicated breast pathologist at our institution and all patients underwent routine stage workup.
Demographic and clinical information were collected from the retrospectively maintained breast cancer research database under institutional review board-approved protocols and included age at the time of diagnosis, family history (FH) of breast cancer (BC) and/or ovarian cancer (OC)in at least 1 first-degree and/or second-degree relative, number of relatives affected with BC and/or OC (first-degree and/ or second-degree relatives only), histopathologic features of tumors, type of surgery, expression of ER, PR, HER2, Ki67, and prognostic information.

| Pathology and immunohistochemistry
All patients who were included in the analysis underwent definitive surgery and had their pathologic specimens reviewed by dedicated breast pathologists. Dedicated breast pathologists performed histopathological examination to determine nuclear grade, estrogen receptor (ER) and progesterone receptor (PR) status, Ki67 labeling index, HER2 status. Assessment of nuclear grade is based on nuclear pleomorphism and mitotic count. A numerical scoring system of 1 to 3 is used to ensure that two factors are assessed independently. ER and PR positivity were determined if ≥10% of nuclei in the tumor stained positive for ER/PR on immunohistochemical analysis. The HER2 staining pattern was divided into 4 groups: 3+ (strong and diffuse staining in >10% of cancer cells), 2+ (moderate and diffuse staining), 1+ (focal staining), and 0. HER2 positive was defined as HER2 staining was 3+ and Fluorescence in situ hybridization (FISH) testing positive of HER2 gene amplification when HER2 staining was 2+. HER2 negative was defined as HER2 staining 0, 1+, and FISH negative when HER2 staining was 2+. Ki67 labeling index was expressed as the percentage of positive cells in each case and a threshold of ≥14% was indicative of a high proliferation index.

| Mutation analysis and statistical analysis
We sent genomic DNA samples to FALCO Biosystems Ltd. (Kyoto, Japan), and direct sequencing was performed, by using SBS (Sequencing by Synthesis) Method and MLPA (Multiplex Ligation-dependent Probe Amplification) Method. All variants detected by direct sequencing were interpreted according to the Myriad Genetics' criteria.
Patient demographics and clinical characteristics have been tabulated and compared among the two groups, which were defined according to BRCA status (non-carrier and BRCA1/2 carrier) and between mutation carriers and noncarriers, using chi-square tests for categorical variables and t tests for continuous variables. Univariate and multivariate logistic regression analyses on BRCA mutation status (carriers vs. noncarriers) were done to identify factors that were predictive of BRCA1/BRCA2 mutations in the patients with DCIS. A Cox proportional hazards model was used to estimate the risks of Ipsilateral breast tumor recurrence (IBTR) and contralateral breast tumor recurrence (CBTR). p values ≤0.05 were considered statistically significant, and all tests were two-sided. Statistical analysis was carried out using the SPSS Statistics 23.0 software (IBM ® ).
Also, another three variants of uncertain significance (VUS) were detected, one in BRCA1 and two in BRCA2 gene (Table 2).

| Mutation carrier rate by patient demographics and clinical characteristics
The median age of 49 patients at the time of diagnosis was 46 years (ranged from 22 to 73 years). The mean interval between date of diagnosis and date of inclusion in the study was 56.92 months (12-174 months). All patients were born in Japan. Table 3 lists the characteristics and FH of cancer of 49 DCIS patients included in our study. Overall, patients with a distribution of 18.4% aged ≤35 years and 81.6% >35 years at first diagnosis. Among BRCA mutation carriers, 44.4% (4/9) aged ≤35 years and 55.6% (5/9) >35 years. For BRCA mutation noncarriers, 12.5% (12/40) aged ≤35 years and 87.5% (35/40) >35 years. Median age of first diagnosis in BRCA mutation carriers is 39 years (32-73 years), and 46 years (22-71 years) in noncarriers. About 69.4% (34/49) of patients had an FH of BC, and 69.4% (34/49) had in at least 1 first-degree or second-degree relative with BC DX. 10.2% (5/49) had an FH of OC, and 28.6% (14/49) of patients did not report any FH of BC or OC. Patients with an FH of BC and BRCA mutation had a higher proportion compared with patients without any FH of BC (26.5% vs. 0%). Patients with an FH of OC and BRCA mutation had similar proportions compared with patients who had no FH of OC (20.2% vs. 18.2%). BRCA mutation carriers were identified in four of 10 patients (40%) who had ≥2 family members diagnosed with BC compared with five of 24 patients (20.8%) who had a single relative affected by BC.
In univariate analyses (Table 3), age at diagnosis was predictive of BRCA mutation status (p = 0.046), the BRCA mutation rate was significantly higher in patients who were younger than 35 years. No significant differences were noted in ER status, PR status, HER2 status or nuclear grade with respect to BRCA mutation status. Subgroup analysis showed BRCA mutation tumors tended to be ER+/PR+ (6/9, 66.7%) and ER+/HER2+ (5/9, 55.6%), whereas no significant difference compared with BRCA mutation noncarriers. Patients who had an FH of BC had a higher risk of having BRCA mutations compared with patients who had no FH of BC (26.5% vs. 0%; p = 0.042), especially patients who had FH of first-or and ER+/HER2+ status (OR 5.034, 95% CI 1.092-23.210, p = 0.038) remained as independent, significant predictors for BRCA mutation. Specifically, patients who had ≥2 relatives with BC were more likely to have BRCA mutations compared with patients who had no relatives with BC. However, the BRCA mutation rate did not differ significantly between patients who had ≥2 relatives with BC versus patients who had a single relative with BC (p = 0.391). Table 3 also listed histological and immunohistochemical characterization of BRCA2 carriers with DCIS in our study. Univariate analysis showed BRCA2 mutation has no relationship with age cut off by 35 years, but among aged ≤35 years DCIS patients, BRCA2 carriers occupied 62.5%, higher than that in aged >35 years patients (37.5%).

Histological characteristics
There were no significant differences in nuclear grade between BRCA2 carriers and noncarriers (p = 0.319). We divided patients into two subgroups by Grade 1 and Grade 2&3, there's no significant difference between BRCA2 carriers and noncarriers (p = 0.215). But it's found that BRCA2 carriers are much higher (50.0%) in Grade 2 and 3 subgroup, which is only 25.6% of BRCA2 noncarriers. It's probably that DCIS with BRCA2 is more frequently moderately or poorly differentiated tumors (Grade 2 and 3).
In our study, it's found that a statistically significant difference in ER-positive tumors among BRCA2 carriers and noncarriers (p = 0.042). About 100% BRCA2 carriers were ER-positive, ER-positive DCIS were more frequent in BRCA2 carriers. But there's no significant difference in PR-positive tumors between BRCA2 carriers and noncarriers (p = 1.000). The frequency of PR expression in BRCA2 mutation DCIS was similar to that in noncarriers. In subgroup analysis, we divided into three subgroups according to ER/ PR status (ER−/PR−, ER+/PR− and ER+/PR+). It's found that BRCA2-associated DCIS tended to be more frequently ER+/PR+ status (p = 0.049). It's found no differences in the expression of HER2 in BRCA2 carriers and noncarriers. But Significant difference was also noted in ER/HER2 status subgroup, DCIS with BRCA2 mutation had higher frequency of ER+/HER2+ status (p = 0.041).
The result of multivariate logistic regression model analysis for BRCA2 mutation showed that ER+/HER2+ status

| Risk factors for Ipsilateral breast tumor recurrence (IBTR) and contralateral breast tumor recurrence (CBTR) in DCIS patients
A total of 25 patients who underwent BCS, 3 (12.0%) suffered IBTR, at a median follow-up of 64 months (range 16-174 months) for survivors, and recurrent lesions were all IBC. The occurrence of IBTR was higher or tended to be higher in patients with higher NG (NG3) (using Cox univariate analysis, p = 0.001). BRCA mutation status was not associated significantly with occurrence of IBTR (p = 0.865), also no significant difference in patients with FH of BC (p = 0.327). Young age (≤35 years), premenopausal status, hormone receptor positive (HR)/HER2 negative and a lack of radiotherapy were not significantly associated with IBTR (p = 0.352; p = 0.251; p = 0.201; p = 0.526, respectively) ( Table 5). No significant result was found in Cox multivariate analyses. Among all 49 cases, CBTR occurred in nine cases (18.4%), five of which involved invasive ductal carcinomas and four involved DCIS. The median period of CBTR among these nine cases was 36 months (range 12-174 months), after surgery for DCIS. We chose age at diagnosis (cut off by 35 years old), BRCA mutation status, menopausal status, nuclear grade, ER status, PR status, HER2 status, FH of BC, ki67 index (cut off by 14% or 30%), subgroup of HR/HER2 status, adjuvant endocrine therapy and irradiation therapy as related risk factors for Cox univariate analysis (Table 6). And we select factors which were p value ≤0.5 in univariate analysis as research factors to be used in Cox multivariate proportional hazard general linear models to estimate risk of CBTR. It's found that ER status was an independent risk factor for CBTR on multivariate analysis (hazard ratio 0.085; 95% CI 0.007-1.047; p = 0.05) (Table 6), CBTR was higher or tended to be occurred in patients with ER negative.

| DISCUSSION
In our study, it indicated an overall 18.4% (17.6% before age 50 years) prevalence of deleterious BRCA1/BRCA2 mutations in high-risk women diagnosed with DCIS, supporting the presence of an in situ phase of carcinogenesis in the development of at least some BRCA-associated breast cancer (Arun et al., 2009). One study evaluated the mutation rate in 10,000 consecutive patients who were referred for genetic testing in Myriad Genetic Laboratories (Frank et al., 2002). The prevalence of BRCA mutations was 13% in women who had DCIS diagnosed before age 50 years versus 24% in women who had IBC. Hall et al. (2010) conducted a crosssectional analysis of the Myriad Genetics BRCA1/BRCA2 database and reported an overall 5.9% prevalence of BRCA1/ BRCA2 mutations in non-AJ patients with carcinoma in situ (CIS) (ductal or lobular). But only a few studies published have described the prevalence of BRCA mutation status in Japanese women with DCIS. Among mutations detected in BRCA1, L63X, and Q934X were reported as founder mutation in Japanese (Ikeda et al., 2001;Lakhani, Easton, & Stratton, 1997;Sekine et al., 2001;Sugano et al., 2008). In our study, Q1721X (c.5280C>T) was detected in only one subject, which has not previously reported nor been listed in the BIC database. Genetic variants of unknown significance G401E (1321G>A) were detected in one subject and also was thus far unreported in the BIC database. In the analysis of BRCA2, Ikeda et al. (2001) showed that BRCA2 5802delT-TAA mutation was considered as common in Japanese breast cancer patients, and Nakamura et al. (Lakhani et al., 1997) reported 5804del4 and R3128X was most frequent mutation. In our study, S2835X, 3036del4, 1506delA, R2318X, I2675V, and 8817insA had been reported in Japanese (Nakamura et al., 2015). K467X (c.1627A>T) was previously reported in a Korean population (Kang et al., 2002), and it's the first time to be detected in a Japanese subject in our study. E1299X (c.4123G>T) was a novel mutational type which was detected in an age 70 years Japanese DCIS patient with luminal B subtype. As for genetic variants of uncertain significance, two mutational types H3056Y (c.9394C>T) and IVS6-2A>G were detected once in our study which were far unreported in the BIC database. IVS6-2A>G had been reported in Japanese (Lakhani et al., 1997), H3056Y (c.9394C>T) was novel mutational type in Japanese. But we did not detect most frequent type of BRCA1/2 mutation in our cohort, the reason is perhaps that DCIS is less commonly in breast cancer, a large-scale cohort study is required to obtain more precise information about founder mutations of DCIS patients in Japan. In our study, cohort of women with pure DCIS who were referred for genetic risk assessment, we identified the predictive factors for BRCA1/BRCA2 mutations. Multivariate analysis revealed that ≥2 family members with BC (OR, 5.242) was one of independent predictors for mutation status. Several studies (Frank et al., 1998;Smith et al., 2007;Claus et al., 2005;Hall et al., 2010) have identified an FH of OC and early onset BC as risk factors for BRCA mutations among DCIS probands. We found that FH of BC was related to BRCA mutation in univariate analyses (p = 0.042), but the association failed to reach statistical significance in multivariate analysis, and FH of OC has no relationship with BRCA mutation. Hall et al. 2010 determined that women who had early onset DCIS had a significantly increased risk of BRCA1/ BRCA2 mutation compared with women who had late-onset disease (aged ≥50 years; OR, 1.5; 95% CI 1.1-2.1). This association was higher in women with very early onset disease (age <40 years vs. ≥40 years; OR, 1.8; 95% CI 1.3-2.3). Conversely, Smith et al. (2007) observed similar mutation rates between patients with DCIS who were diagnosed at age <50 years and with those who were diagnosed at a later age. In our study, the BRCA mutation rate was significantly higher in patients who were younger than 35 years (p = 0.046), but it was also predictive of mutation status in multivariate analysis (OR 0.149, 95% CI 0.023-0.954, p = 0.045). ER status, PR status, HER2 status, and Ki67 index (cut off by 14% or 30%) did not differ between BRCA mutation carriers and noncarriers. But subgroup analysis showed that ER+/HER2+ status (OR 5.034, 95% CI 1.092-23.210, p = 0.038) remained as a significant predictor for BRCA mutation. So it's suggested that DCIS patients with total relatives with BC DX ≥2, age at diagnosis ≤35 years and ER+/HER+ status have high risk of BRCA mutation and could be recommended to receive genetic counseling and BRCA testing.
BRCA2 tumors are more frequently moderately or poorly differentiated carcinomas (grades 2 and 3) (Agnarsson et al., 1998;Lakhani et al., 1997Lakhani et al., , 2000Lynch et al., 1998). In our study, no significant differences were found in grade between BRCA2 carriers and noncarriers (p = 0.319). But it's found that BRCA2 carriers are much higher in Grade 2 and 3 compared to that of BRCA2 noncarriers. It's similar to BRCA2 carriers in invasive carcinomas. In BRCA2 tumors, in contrast to BRCA1 tumors, it has been shown that the percentage of ER-positive cases. Foulkes et al. (2004) analyzing tumors from BRCA2 positive patients <45 years, found a statistically significant difference in the percentage of ER-positive tumors among BRCA2 carriers and noncarriers. The percentage of ER-positive tumors was higher in BRCA2 tumors; however, there was no difference found in a subanalysis of tumors from patients >55 years. Our data indicated that BRCA2 carriers with DCIS were tend to be ER-positive (p = 0.042) no matter with the age of patients, and subgroup analysis showed BRCA2 tumors with DICS was higher in expression of ER+/ PR+ (p = 0.049). Data on HER2 expression in BRCA2-associated tumors vary from series to series, probably as a consequence of differences in the techniques employed. We found no significant difference between HER2 status and BRCA2 mutation, but subgroup analysis suggested that ER+/HER2+ could be an independent risk factor for BRCA2 mutation, and it's also one of independent predictive factors for BRCA mutation in our study. Since BRCA2 has a major role in DNA repair, its suppression is thought to induce unrepaired DNA lesions, which cause cell cycle arrest by activating checkpoint signaling. So we also found BRCA2 carriers with DCIS were tend to be lower proliferation (<30%), whereas has no significant difference.
The proportion of DCIS among surgically resected breast cancers is reported to be 20% in Western countries and nearly 10% in Japan (Burstein, Poluak, Wong, Lester, & Kaelin, 2004;Cancer Statistics in Japan, 2013;Ernster et al., 2003). Pure DCIS in itself is not a life-threatening disease, and the local recurrences if that appear as DCIS do not influence the overall survival rate of patients. True DCIS will theoretically not metastasize to regional lymph nodes or relapse in a distant organ, and thus the management of DCIS patients focuses on local control of the primary lesion and early detection and treatment of both IBTR and CBTR (Fisher et al., 2002(Fisher et al., , 1999. BRCA1/BRCA2 mutations have been shown to indicate a higher susceptibility to develop BC. Individuals who carry one of these mutations have a 43%-84% risk of developing BC, and up to a 65% risk for CBC (Finkelstein et al., 2006;Ford et al., 1998). But in our study, BRCA mutation status was not associated significantly with occurrence of IBTR. It indicated that, BCS might be a treatment option for DCIS patients with BRCA mutation, for they may be more sensitive to radiation (Garcia-Etienne et al., 2009;Kirova et al., 2010). But we need additional data of more research samples to support this conclusion. It's also found that CBTR rate was higher or tended to be higher in DCIS patients with ER negative, but no relationship with BRCA mutation status. Further study was needed to discuss whether BRCA mutation is the independent factor for CBTR in DCIS patients, and it's very important for DCIS women with BRCA mutation whether or not to choose contralateral prophylactic mastectomy.

| CONCLUSION
DCIS is equally as prevalent in patients who were BRCA mutation carriers as in high familial-risk women who were noncarriers, but occurs at earlier age. BRCA2 carriers have higher incidence in DCIS than that of BRCA1 carriers, and tend to be higher grade and more frequently ER positive and lower proliferation. Total relatives with BC DX ≥2, age at diagnosis ≤35 years and ER+/HER2+ might be independent predictors for BRCA mutation in Japanese women with DCIS and patients of these risk factors should be recommended to receive genetic counseling and BRCA testing.