Genetics and genomics in Peru: Clinical and research perspective.

Peruvians currently preserve in their DNA the history of 2.5 million years of human evolution and 150,000 years of migration from Africa to Peru or the Americas. The development of Genetics and Genomics in the clinical and academic field is shown in this review.


| PERUVIAN DEMOGRAPHICS
Peru is a pluricultural and ethnically diverse country located in middle west of South America. It is the fourth largest country (1,285,216 km 2 ) in area in South America, bordered in the north by Ecuador and Colombia, in the south by Chile, south east by Bolivia, by Brazil in the east and by the Pacific Ocean in the west. Peru has one of the most diverse climates, geographical and ecological in the world, from tropical or subtropical dessert to glacial in highland mountains to Amazon jungle. Peru is divided into 24 geopolitical regions with a central government. According to last national census in 2017, Peruvian populations accounts for 31,237,385 inhabitants (Instituto Nacional de Estadistica e Informatica [INEI], 2017). Most of Peru's population lives in the Coastal area, with almost one third of the nation's population living in Lima, the largest city with approximately 11 million people. Rural and urban population has changed over the last 50 years due to migration waves in the 1950s from rural to urban sites mainly located in the coast causing decrease or disappearance of native communities.
The Peruvian health care system in Peru includes five different subsystems: (a) Ministry of Health that provides services to people categorized as poor and extremely poor (around of the population) covered by SIS (Integral Health insurance), (b) Social security subsystem (EsSalud) administered by Labor and Employment Minister that provides services to formal employees and their dependents (26.7% of population), (c) Armed Forces subsystem run by Minister of Defense, (d) Police forces subsystem administered by Ministry of Interior and (e) the Private sector (Alcalde-Rabanal, Lazo-González, & Nigenda, 2011;Ypanaqué-Luyo & Martins, 2015). Within the most prevalent diseases in Peru, upper respiratory infectious (viral or bacterial) diseases have the highest morbidity rates and cancer (gastric, cervical, uterine, breast, prostate, and lung) the highest mortality rates (INEI, 2015). This gives the unique opportunity for diverse genetic studies in the most prevalent infectious and non-infectious diseases afflicting the Peruvian population.

GENETICS AND GENOMICS
Genetic services in Peru are scarce but growing despite limitations in infrastructure and qualified human resources. The very few public genetic services available in the country offer clinical care and genetic counseling provided by medical geneticists and other specialists with training on genetics. Available molecular diagnosis is mostly based on cytogenetic techniques, PCR-based and Sanger sequencing technologies. There are 21 accredited medical geneticists in national registry of the Peruvian Medical college of Physician (https://cmp.org. pe/conoce-a-tu-medico/) most of them working in public hospitals within the Capital city. Training in human genetics and medical genetics are scarce with only one accredited medical genetics residency program for physicians, one postgraduate program on human genetics and one biology, genetics and biotechnology graduate program for molecular biology specialists. Genetic counseling is currently offered by medical geneticists and other specialists with training in genetics; however, there is no formal accredited Genetic counseling program in Peru. In addition, national and international collaborations have been crucial specially in bioinformatic analysis due to in Peru there is still no university bioinformatics career, the trainings only are part of courses, seminars inside and outside our country.

| Newborn screening
There are isolated hospital initiatives for newborn screening in Peru. By 2012, a law state the national interest for having a Universal Newborn screening program, prioritizing screening for congenital hypothyroidism (CH), congenital adrenal hyperplasia (CAH), phenylketonuria (PKU), cystic fibrosis, hearing loss and congenital cataract (Galán-Rodas, Duenas, Obando, & Saborio 2013). Up to now, there are independent newborn screening programs in main hospitals of Ministry of Health, Social security (EsSalud) subsystem and private sector. The program from EsSAlud started back in 2011 (INEI, 2015), including screening for CH, PKU, CAH, and galactosemia (GAL) available in every single EsSalud healthcare center across the country. The incidence of CH, CAH,PKU,and GAL was 1:3,211,1:22,207,1:46,970,and 1:37,576 (Cruz-Ticona et al., 2015), The most common birth defects are as follows: congenital heart defects, Down syndrome, cleft lip and/or palate, hydrocephaly and neural tube defects.

| Pediatric genetics
Clinical genetics started at the Hospital Guillermo Almenara Irigoyen in 1968, with clinical dysmorphology and karyotype diagnostic test without banding (Delgado-Matallana, 2000). Furthermore, clinical dysmorphology flourished at Instituto Nacional de Salud del Niño-Breña (INSN-Breña), with the advantage of being the representative national institute for children's health. Over the years, all this experience was published as a unique book in dysmorphology linking patient (authorized by parents) images and a succinct clinical and molecular explanation of diseases. This recompilation entitled "Atlas de Dismorfología Pediátrica" (Atlas of Pediatric Dysmorphology) gathered more than 200 syndromes and recognizable clinical cases with Peruvian patients' pictures (Klein, Gallardo, Chavez, & Abarca, 2012). Furthermore, INSN-Breña is one of the biggest national institutions where patients from Peru and within the MINSA healthcare system receive diagnosis and management. In Table 1, we can observe the frequency of diagnosed genetic disorders in this institution at the Service of Genetic and Inborn Error of Metabolism.
Chromosomal microarray analysis, is the first-tier clinical diagnostic test for patients with intellectual disability, autism spectrum disorders and congenital anomalies (Miller et al., 2010). This technique was implemented and developed at INSN-Breña, revealing etiology in more than 50% of patients with intellectual disability and development delay (Abarca-Barriga, Chávez Pastor, Trubnykova, Vásquez, & Poterico, 2017), with a previous clinical genetic consultation. Furthermore, we found novel variants and demonstrated how loss of copy number variants (CNV) resulted in homozygous deletion in MPLKIP (OMIM #609188) and SUGCT (OMIM #609187) genes, which are causes of Trichothiodystrophy type 4 and glutaric aciduria type 3, respectively (Serna-Infantes et al., 2018). Also, it allowed us to describe a patient with 18p−/18q+ syndrome, adding to the phenotype by describing the persistent microscopic hematuria and aortic pseudocoarctation with descending aorta walls thickening, suggesting Takayasu's arteritis .
Collaboration with European geneticists allowed the report of novel CNVs of the Sonic hedgehog (SHH) limb enhancer ZPA regulatory sequence (ZRS) on chromosome 7q36 and its relationship with specific polysyndactyly syndromes. These microduplications correlated to Laurin-Sandrow syndrome and Haas-type polysyndactyly, according to the ZRS region length (Lohan et al., 2014 (Hamilton et al., 2018), and Shawaf-Traboulsi syndrome (Abarca . Last, international collaboration permitted reports of two common genetic syndromes-that is, Noonan (Kruszka et al., 2018) and Williams-Beuren (Kruszka et al., 2018)-with clinical geneticists of the National Human Genome Research Institute of The National Institutes of Health; and with other geneticists of different sites in the globe, contributing to the worldwide clinical genetics area. A political decision allowed the Peruvian population to have a novel institution for children's healthcare. This specialized institute is called Instituto Nacional de Salud del Niño San Borja (INSN-SB) and is a national reference center for complex cases requiring surgical treatment or transplantation. The Genetics Service started in 2015, with a rapid development in clinical and laboratory activities.
The clinical genetics consultations at INSN-SB are carried out by five clinical geneticists, estimating 400 patients attending every month approximately. The most frequent diagnosis is Down syndrome. Evaluation includes an increasing number of patients with diagnosis of cardiac anomalies (e.g., heterotaxy, dilated cardiomyopathy), neurological (e.g., epilepsy, intellectual disability, hearing loss) or behavioral disorders (e.g., autism).
Cytogenetic analyses have been implemented since the establishment of the Genetics Service at INSN-SB, mostly for constitutional analyses. In addition, there are bone marrow studies of children with leukemia, and chromosome fragility test for diagnosis of Fanconi anemia-being the unique center doing this test in Peru. Since 2016, more than 500 blood and almost 200 bone marrow samples have been analyzed. On the other hand, more than 600 samples have been analyzed for detection of genetic alterations in patients with haematological neoplasms. Ongoing research projects with institutional investment involve cardiac anomalies and pharmacogenetics in children with leukemia.
INSN-SB is working on administrative and logistics issues for obtaining equipment and human resources for the implementation of genetic panel test using next-generation sequencing (NGS) in pediatric diseases, such as cardiac anomalies, bone marrow failure syndromes, among others.

| Cancer genetics
Clinical genetics and counseling in oncology started in 2006 at the Instituto Nacional de Enfermedades Neoplásicas (National Institute of Neoplastic Diseases-INEN) as a research initiative with public funding. Since that time, genetic consultations have been increasing not only in numbers but also in clinical conditions with internal and external collaborations, as more non-genetics physicians are aware of hereditary conditions. Moreover, the clinical training on genetics has INEN as the unique node for future medical geneticists.
More than 1,500 patients have attended a clinical geneticist consultation; even if reference to the genetic consultation at INEN is <5% annually. For instance, breast cancer represents the more frequent consultation at the Unit of Molecular Biology and Genetics at INEN (Manrique, Sullcahuamán-Allende, & Limache-García, 2013). Collaboration with foreign institutions such as the private Clinic City of Hope, lead the Peruvian clinical geneticists to be part of clinical research on hereditary or familial cancer in Peru. Abugattas et al. (2015) depicted in an unselected cohort of women with breast cancer that 5% carried BRCA1 (OMIM #113705) or BRCA2 (OMIM #600185) specific genetic T A B L E 1 (Continued) alterations (Latino-variants panel). BRCA1 185delAG was the most frequent variant in these patients. However, the technology employed to identity genetic chances involved a catalog of Latino-variants confirmed by Sanger sequencing, not involving other types of pathogenic genetic changes. Long-standing collaboration with City of Hope has been allowing-through a continuous research project-reports of germline variants of patients attending the genetic counseling consultation at INEN. Genetic information helps geneticists to give an appropriate genetic counseling, and an appropriate management. Current state-of-the art technologies (i.e., NGS) locally implemented at INEN would allow more patients for evaluation and genetic counseling. On the other hand, clinical and laboratory data have allowed Peruvian geneticists to establish international collaborations not only on the area of hereditary of familial breast cancer syndromes (Abugattas et al., 2015), but also on colorectal cancer (CRC) (Rossi et al., 2017) and gastric cancer (GC) predisposition (Slavin et al., 2017), among others.
Moreover, other clinical international collaborations have arisen on CRC and GC. For instance, a large study which included 362 GC patients from Lima, Peru fond that IRF4 (OMIM #601900), ELMO1(OMIM #606420), CLIP4 (OMIM #605736) and MSC (OMIM #603628) promoter methylation coupled with a GDMI > 4 are useful molecular tools for GC risk stratification in endoscopic biopsies (Pirini et al., 2017). An interesting study in native Peruvians (Quechuas from the Central Andes) did not found an association between candidate SNPs and GC (Zamudio et al., 2016).

| Genetics in autoimmune diseases
Several manuscripts have been published on genetic aspects of autoimmune diseases in Peruvian populations; most of them were collaborative efforts. These studies involved patients of tertiary-level hospitals, mainly the Hospitals Guillermo Almenara Irigoyen and Edgardo Rebagliati Martins from the Social Security System, and in the Hospital Cayetano Heredia from the Ministry of Health System. Specific studies relied on autoimmune disorders, such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), spondyloarthritis (SpA) and autoimmune hepatitis.
A new variant HLA-DRB1 (OMIM #142851) (14:02) was reported in the first of this sort of Peruvian studies on RA in 2001 (Castro et al., 2001). A meta-analysis of HLA-DQA1 (OMIM #146880) polymorphism in Latin American populations included two Peruvian reports on this issue: from the Hospital Guillermo Almenara Irigoyen and the Air Force Army Hospital (Delgado-Vega & Anaya, 2007). Another Latin American collaboration-Genome of RA network consortium (GENAR)-found that the more Amerindian ancestry, the lower odds of fulfilling several RA criteria (Sánchez et al., 2017). On the other hand, Amerindian descendants could have new genetic loci related to RA (Herráez et al., 2013).
In SLE, two Peruvian hospitals (Hospitals Guillermo Almenara Irigoyen and Edgardo Rebagliati Martins) are part of GENLES (Genoma de Lupus Eritematoso Sistémico Network consortium/Genome of SLE network consortium) which is the biggest genetic study in Latin American populations. This cohort has reported that Amerindian ancestry relates to higher genetic load of SLE risk alleles compared to Caucasian (Sanchez et al., 2010) and to a more severe disease (Sánchez et al., 2012). Nevertheless, when two cohorts with a similar ancestry were compared (LUMINA [Lupus in Minorities: Nature vs. Nurture] and GLADEL [Grupo Latino Americano de Estudio de Lupus/Latin American Group for the Study of SLE]), outcome differences could not be explained by Amerindian ancestry (Ugarte-Gil et al., 2015). Furthermore, GENLES, in collaboration with other cohorts from US and Europe, reported several new loci (Langefeld et al., 2017;Ugarte-Gil et al., 2015). As part of a Peruvian SLE Cohort named the Almenara Lupus Cohort, a study of hypomethylation in SLE patients has been started in collaboration with the University of California in San Francisco (UCSF).
Regarding SpA and its main genetic risk (i.e., HLA-B27 [OMIM #142830]), a Peruvian population from Hospital Cayetano Heredia in 1981 (Alarcon et al., 1981) reported a low frequency of HLA-B27 antigen. This asseveration affects SpA Peruvian patients (Cuchacovich et al., 2002), explaining in part the low proportion of Peruvian patients suffering with SpA with this genetic marker. Recently, Hospital Guillermo Almenara Irigoyen has found that ankylosing spondylitis patients in Peru not only have a lower prevalence of HLA-B27 (<30%) but also a high prevalence of HLA-B39 (almost 50%); the latter variant is expected to have a similar effect than HLA-B27 in the pathogenesis of the disease

| Neurogenetics research
Neurogenetics in Peru offers specialized care for patients and families affected with repeat expansion disorders and other familial neurodegenerative disorders. The only neurogenetics center in Peru, based at the Instituto Nacional de Ciencias Neurologicas (National Institute of Neurological Sciences), was founded in 1995. The Neurogenetics center provides specialized care for patients and families affected with genetic and orphan neurodegenerative disorders (Cornejo-Olivas, . By 2000, the CAG repeat analysis of HTT (OMIM #613004), the gene responsible for Huntington disease became the first molecular protocol implemented at this center (Walker et al., 2018). As a national reference center, the outpatient clinic receives patients and families from different regions of Peru, mostly affected with Huntington disease, inherited ataxias, myotonic dystrophy type 1 and other muscular dystrophies, inherited neuropathies, familial Parkinson and Alzheimer disease among others (Cornejo-Olivas, Mazzetti et al., 2015;Walker et al., 2018). Since 2009, neurogenetic care combines multidisciplinary clinical approach, molecular diagnosis, and clinical research.
Large samples of Huntington and inherited ataxia cases provide a better understanding of genetic epidemiology and its impact in public health in these disorders. The first report of Huntington disease, also known as CHOR-HTT, in Peru traces back to 1950, describing a large family from southern Peru (Torres, Mori-Quispe, & Mendoza-Cabanillas, 2006). Further reports during the late 80s and early 90s highlighted a surprisingly high frequency of cases coming from the Cañete Valley-southern Lima, 200 km south to the Capital city (Cuba, 1986;Cuba, Castro, & Benzaquen, 1983;Cuba & Torres, 1989). The large cohort of CHOR-HTT families in Peru have been made significant scientific contributions in the field including genotype-phenotype correlations of late-onset cases (Cornejo-Olivas,  and Huntingtonlike disorders (Walker et al., 2018), haplotypes analysis suggesting an Amerindian origin of the HTT mutation (Kay et al., 2017) and health-cost analysis highlighting the dramatic burden of CHOR-HTT in the Peruvian population. Since 2012, an outreach clinic, combining both home visits and consultations at primary care center, is offering multidisciplinary care to CHOR-HTT patients and families from the Cañete valley (Vishnevetsky et al., 2018). Inherited ataxic cases followed over the past 10 years is demonstrating a different distribution of dominant ataxias with relative high frequency of spinocerebellar ataxia type 10 or SCA10 (OMIM #603516) in Peruvian population (Bampi et al., 2017;Gheno et al., 2017).
Genetics of Parkinson disease (PD) demonstrates the transcendence of studying populations with different ancestries when looking for pathogenic variants. Since 2007, as part of the Latin American Research Consortium on the Genetics of Parkinson's Disease (LARGE-PD), the study of genetics of PD in Peruvian population contributed to increased knowledge of PD. The LARGE PD cohort in Peru facilitate the discovery of novel mutations in PARKIN (OMIM #602544) gene (Cornejo-Olivas,  as well as addressing the frequency and genotype-genotype correlations of pathogenic variants of the LRRK2 (OMIM #609007) gene in Mestizo population from Latin America Mata et al., 2009Mata et al., , 2011.

| Genetics and pharmacogenetics in tuberculosis
Tuberculosis (TB) is an infectious disease with high incidence in Peru. It has been shown that TB can occur at different rates among different races, ethnicities, and even families indicating a genetic predisposition to tuberculosis susceptibility. Different studies have been done to identify genetic polymorphisms related to severity and progression of TB disease. Two-locus genotype −2,518 macrophage chemoattractant protein CCL2 (OMIM #158105) GG and −1607 matrix metalloproteinase MMP1(OMIM #120353) 2G/2G has been found to promote the expression of hyperinflammation in response to Mycobacterium tuberculosis infection, inducing extensive tissue damage and a severe TB disease (Ganachari, Guio, Zhao, & Flores-Villanueva, 2012). Regarding TB progression, some variants located near the enhancer region on chromosome 3q23 also have been found (Luo et al., 2018). Together with the data obtained from the Peruvian Genome Project and these previous studies, it will be possible to define the origins and distribution of these new polymorphisms associated with transmissible and non-transmissible diseases in Peru: integrating Population Genomics knowledge to Public Health. How our genetic diversity affects our pharmacological therapy is the basis of pharmacogenomics where the genotype of an individual is identified to determine his or her drug metabolic phenotype, predicting the probability of his or her response and minimizing the adverse reactions to achieve a personalized treatment (Rohrer Vitek, Nicholson, Schultz, & Caraballo, 2015;Weinshilboum & Wang, 2004). Even though metabolic genotypes and phenotypes have been widely studied in Caucasian and Asian populations, in many Latin American populations this key information is still missing. The Peruvian Mestizo population's unique admixture between multiple Native American communities in its region (Harris et al., 2018) results in a distinct population or populations that do not allow for assumptions from existing pharmacogenomics data. Up to date, only two pharmacogenomic studies have included Peruvian Native American samples (Bisso-Machado et al., 2016;Fuselli et al., 2007). Due to Peru's high rate of TB, researchers are directing their focus toward the role of pharmacogenomics on TB treatment by starting with the identification of the genetic polymorphisms affecting isoniazid and rifampicin (first-line and second-line antituberculous drugs) metabolism and thus affecting the efficacy of the treatment and the induction of adverse effects. Two main research groups (Dr. Heinner Guio's group at the Peruvian National Institute of Health in collaboration with the Peruvian National Health Strategy for the Prevention and Control of Tuberculosis [ESPNCT [ Guio, Levano, Sánchez, & Tarazona, 2015], and Dr. Ricardo Fujita's group at the San Martin de Porres University) are identifying the frequency in the Peruvian population of genetic polymorphisms in crucial drug metabolizing enzymes, NAT2 (OMIM #612182) and CYP2E1 (OMIM #124040), that result in phenotypes that could cause either a decrease or an increase in anti-tuberculous drug concentration affecting the efficacy of the treatment. Genotypic and phenotypic assessment will become essential for the evaluation protocol to provide an adequate personalized treatment that increases efficacy and reduces side effects. The concept of "one dose for all" strategy in TB treatment could be an alternative to avoid mono or multidrug resistance in the Mycobacteria tuberculosis.

| An example of the role of Peruvian universities in genetic and genomic research
The Centro de Genetica y Biologia Molecular de la Universidad San Martin de Porres (CGBM-USMP) has conducted studies on rare Mendelian diseases, chronic diseases, pharmacogenetics, immunogenetics and ancestry in Peruvian populations. As expected, in a mestizo population, there are germline variants reported in Europe, Africa, and Asia, whereas other novel variants, probably of Amerindian origin are also frequently found. Some Mendelian diseases studied by this university-research center are ocular, neuromuscular diseases, cancer, among others.
CGBM-USMP studies of glaucoma in different Peruvian families have allowed refinement of the GLC1B (OMIM #606689) locus in the 2q12-q13 chromosomal region, and a candidate gene for the disease is currently being assessed. The study of MYOC (OMIM #601652) (locus GLC1A) has identified both known mutations in European and African-American populations, as well as novel mutations. Interestingly, these variants are correlated with African ancestry (Chincha and Cañete) and the novel are recurrent in Peru, probably indicating an autochthonous founder effect (Guevara-Fujita et al., 2008;Mendoza-Reinoso et al., 2012). Another disease studied at CGBM-USMP is retinitis pigmentosa-which has a genetic heterogeneity, with more than 70 genes-in approximately 100 Peruvian families, having identified the causative mutation in 30 of them. Notably, the Parán community-enclave in a rural area of Lima-with 60 of the 350 inhabitants have retinitis pigmentosa due to germline variants in the RPGR (OMIM #312610) gene located on the chromosome X.
CGBM-USMP team has assessed more than 300 Peruvian patients with muscular dystrophies, most of them with clinical diagnosis of Duchenne disease. The most common type of mutation are exon deletions, although much lower than in other studies (38% vs. 55%-70%). On the other hand, nonsense mutations have a higher prevalence (30%) compared to other reports (10%-15%). It is not known the reason of these results, but it is possible that it is due to founder effects. This group's research on Peruvian patients with breast cancer has been depicting interesting results. A first analysis of BRCA1 and BRCA2 by Sanger sequencing and multiplex ligationdependent probe amplification (MLPA) in 18 families with suspicion of hereditary breast and ovary cancer syndrome (HBOC), demonstrated four pathogenic variants. Three of these variants have been previously reported (BRCA1: c.302-1G>C and c.815_824dup10; BRCA2: c.5946delT), but they are absent in the Latino-variants panel mentioned above. On the other hand, a duplication of adenines in exon 15 in BRCA1 (c.4647_4648dupAA, ClinVar SCV000256598.1) and BRCA1 c.4647_4648dupAA were novel pathogenic variants. Interestingly, two exonic and four intronic variants of unknown significance, some of them found recurrently in HBOC Peruvian families and are absent in normal controls and databases (Buleje et al., 2017). Additionally, other universities such as the Universidad Peruana Cayetano Heredia and the Universidad Mayor de San Marcos have made important contributions in genetic and genomic research.

| Fundamentals of biobanking for translational oncology research
Instituto Nacional de Enfermedades Neoplásicas is a public health center, specialized in the prevention, detection, diagnosis, treatment, and rehabilitation of patients affected by neoplastic diseases or malignant tumors. INEN have implemented genetic platforms for diagnosis and research with international collaborations. The administrative area directly linked to development of oncogenomic scientific research in the institute is the Tumor Biobank of INEN (BTT).
BTT collaborates with diverse scientific organizations, hence serving as a platform for translational research in oncology, with implemented laboratories with capacities in: molecular biology, cell culture, image registration, and histotechnology.
In collaboration with the Institute of Research for Development (IRD)-France, BTT researchers described in Peru an unusual clinical presentation of hepatocellular carcinoma (HCC) with noteworthy characteristics compared to those observed in other regions of the world. We described a younger population displaying an abnormal juvenile mean age of 25 years old, and large tumor exceeding 10 cm diameter, and only 11% of HCCs occurred in the setting of cirrhosis (Bertani et al., 2013;Ruiz et al., 2016).
In order to deepen our understanding of the molecular processes ongoing in Peruvian liver tumors, mutation spectrum analysis was carried out on hepatocellular carcinomas from Peruvian patients. Thirty-one exons covering 17 kb in nine genes considered as mutation hotspots in HCC were analyzed: ARID2 (OMIM #609539), AXIN1(OMIM #603816), BRAF (OMIM #164757), CTNNB1 (OMIM #116806), NFE2L2 (OMIM #600492), H-RAS (OMIM #190020) K-RAS (OMIM #190070), N-RAS (OMIM #164790), and TP53 (OMIM #191170) (Marchio et al., 2014). Such genotypic alterations are unusual elsewhere, as CTNNB1 mutants were found preferentially in non-HBV-associated HCC. The uncommon genetic profile of Peruvian HCC was further confirmed by the conspicuous excess of deletions present in the mutation spectrum, a pattern rarely observed in sporadic cancer and never described so far in HCC (Marchio et al., 2014). The bimodal age-based distribution, the young age of a distinct subgroup, the low rate of associated cirrhosis, and a peculiar mutation spectrum delineate an unusual tumoral process ongoing in the Peruvian population.
In the last years, the pediatric department of INEN received an unusually high number of children with primary intracranial sarcomas. Most of them are fusocellular and pleomorphic high-grade sarcomas. BTT with international collaboration allow analysis with NGS of 11 pediatric intracranial sarcomas NOS from Peru; revealing DICER1 (OMIM #606241) pathogenic variants in terms of a distinct methylation signature and the presence of DICER1 alterations (Koelsche et al., 2018).

| Understanding molecular behavior for genetics
Genetic and genomic medicine need the feedback-and vice versa-from basic science for understanding molecular, cellular, and intercellular processes and applications to clinical settings (Shamanna, Croteau, Lee, & Bohr, 2017). Werner protein (WRNp) represents an excellent example of this, with a scientific node in Peru with international collaboration. Werner syndrome (WS) is a rare autosomal recessive and heritable disease with unknown prevalence in Latin American countries, among others. This disease is associated with truncation or non-functional variants of the protein (Orphanet, 2012). Therefore, patients with this condition are prone to premature aging: that is, metabolic disturbances, cardiovascular defects and neoplastic diseases (Orphanet, 2012;Shamanna et al., 2017). Among the latter, a higher risk of a small subset of neoplasms has been reported, with several specific types of cancer reported like thyroid cancer, malignant melanoma, meningioma, soft tissue sarcoma, osteosarcoma, breast cancer, and leukemia (Goto & Ishikawa, 1996;Lauper, Krause, Vaughan, & Monnat, 2013).
It is well known that WRNp is a nuclear protein whose main function is to protect genome integrity (Rossi, Ghosh, & Bohr, 2010), but it has also been detected in the cytoplasm (Kobbe et al., 2002;Shamanna et al., 2016;Slupianek et al., 2011). However, the cytoplasmatic WRNp behavior remains unknown. Tokita et al. recently reported in a genomic evaluation that tumors of patients with WS depicted slight differences in senescence, mitochondrial dysfunction and telomere attrition markers (Tokita et al., 2016). Interesting but uncertain-according to specific population findings-clinical prediction related to WRN dysregulation in some diseases such as cancer, remains to be clarified with WRNp variants different from the truncating or non-functional. For instance, reports have suggested a link between this sort of variants and breast cancer (Wang et al., 2009;Wirtenberger et al., 2006). Indeed, more research of WRNp needs to be carried out to clarify clinical consequences and therapeutic targets development.
Peruvian Professor Iglesias-Pedraz and his foreign colleagues have been trying to elucidate the WRNp function and behavior, demonstrating that immortal cancer cells harboring stable and conditional silencing system for WRNp, reduce de novo protein synthesis prior to any detectable stress response, weakening the cellular proliferation. This alteration is not due to a defect in gene expression nor protein instability (Li et al., 2014), but due apparently to a defect in the proper protein synthesis in WRN-depleted cancer cells.. Additional findings have been observed by these scientists; for example, they found WRNp located in the cytoplasm co-sediments with ribosomal-enriched complex (unpublished data). This finding was noticed years before by Motonaga et al. (2002) in human tissues and recently, Lachapelle et al. (2011) have identified interaction of WRNp with several components of translational machinery. The aforementioned suggests that the collaborating-group of Professor Iglesias-Pedraz could be immersed in an exciting and novel scientific road regarding WRNp.

| PERUVIAN GENOME PROJECT
Modern Peruvians carry in their DNA the history of 2.5 million years of evolution and 150,000 years of migration from Africa to Peru or the Americas. Climatic changes and high altitude, different pathogens and interaction with other ethnic groups, have sculpted the genetic variants and genetic expression in the modern Peruvians. The Andes mountains divide Peru in three regions (coast, highlands, and jungle). One third of the Peruvian population lives above 2,500 m.a.s.l. and over 50,000 people reside above 4,000 m.a.s.l (Macsorley, 2001). On the other hand, 60% of the Peruvians that live in the jungle are exposed to different intestinal parasites leaving a particular human being homeostatic condition. Thus, Peruvians represent an unexplored genomic book with unknown genome-environment interaction data.
By 2011, the Peruvian National Institute of Health (Instituto Nacional de Salud del Peru) started the Peruvian Genome Project lead by Dr. Heinner Guio in collaboration with Timothy O'Connor from University of Maryland and Dr. Tarazona from Universidade Federal de Minas Gerais. Despite disparities in research cost (e.g., equipment and supplies) as compared to US (more than 2.5 times) (Sirisena & Dissanayake, 2018), a total of 280 genomes from 17 Native and 13 Mestizo populations have been analyzed (150 genomes were sequenced using whole genome sequencing and 130 using genotype array platform). This study represents the most extensive Native American sequencing project to date. The analysis shows that our Mestizo populations have 60%-70% native genes-in some geographical locations more than 80%. Most of the newly identified SNPs are missense (~60,000) and come from native communities (Harris et al., 2018). Our data demonstrate a high Native ancestry component in the Peruvian population, even as compared to the results obtain in the Mexican genomic project (Belbin, Nieves-Colón, Kenny, Moreno-Estrada, & Gignoux, 2018). Additional studies in genomics of Peruvian populations will help us to understand these new SNPs (Elhaik et al., 2014;Sandoval et al., 2013). No doubt, these new findings from the Peruvian Genome project will enhance genetic research and medical genomics not only for Peruvians, but for Hispanics and Latinos.

| CONCLUDING REMARK S
Peru has been working on genetics and genomics despite the lack of national investment. Multidisciplinary healthcare providers have been contributing to the area of genetics in Peru. We have highlighted several examples of achievements in the clinical and research arenas. Moreover, as the number of well-trained professionals increases-in house and abroadcontributing with national and international collaborators, research, and clinical experiences will increase in quality and extension. All the research data shown in this review could not have been done without local and/or international collaborations. In these times where lots of standardized and incoming technologies and data are emerging, collaboration is extremely important for research and its translation to the public health system, helping in the implementation of precision medicine.