Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNA

Three mantras often guide species and ecosystem management: (i) for preventing invasions by harmful species, ‘early detection and rapid response’; (ii) for conserving imperilled native species, ‘protection of biodiversity hotspots’; and (iii) for assessing biosecurity risk, ‘an ounce of prevention equals a pound of cure.’ However, these and other management goals are elusive when traditional sampling tools (e.g. netting, traps, electrofishing, visual surveys) have poor detection limits, are too slow or are not feasible. One visionary solution is to use an organism’s DNA in the environment (eDNA), rather than the organism itself, as the target of detection. In this issue of Molecular Ecology, Thomsen et al. (2012) provide new evidence demonstrating the feasibility of this approach, showing that eDNA is an accurate indicator of the presence of an impressively diverse set of six aquatic or amphibious taxa including invertebrates, amphibians, a fish and a mammal in a wide range of freshwater habitats. They are also the first to demonstrate that the abundance of eDNA, as measured by qPCR, correlates positively with population abundance estimated with traditional tools. Finally, Thomsen et al. (2012) demonstrate that next-generation sequencing of eDNA can quantify species richness. Overall, Thomsen et al. (2012) provide a revolutionary roadmap for using eDNA for detection of species, estimates of relative abundance and quantification of biodiversity.

Three mantras often guide species and ecosystem management: (i) for preventing invasions by harmful species, 'early detection and rapid response'; (ii) for conserving imperilled native species, 'protection of biodiversity hotspots'; and (iii) for assessing biosecurity risk, 'an ounce of prevention equals a pound of cure.' However, these and other management goals are elusive when traditional sampling tools (e.g. netting, traps, electrofishing, visual surveys) have poor detection limits, are too slow or are not feasible. One visionary solution is to use an organism's DNA in the environment (eDNA), rather than the organism itself, as the target of detection. In this issue of Molecular Ecology, Thomsen et al. (2012) provide new evidence demonstrating the feasibility of this approach, showing that eDNA is an accurate indicator of the presence of an impressively diverse set of six aquatic or amphibious taxa including invertebrates, amphibians, a fish and a mammal in a wide range of freshwater habitats. They are also the first to demonstrate that the abundance of eDNA, as measured by qPCR, correlates positively with population abundance estimated with traditional tools. Finally, Thomsen et al. (2012) demonstrate that next-generation sequencing of eDNA can quantify species richness. Overall, Thomsen et al. (2012) provide a revolutionary roadmap for using eDNA for detection of species, estimates of relative abundance and quantification of biodiversity.
Keywords: biodiversity assessment, biosecurity, eDNA, invasive species, natural resource management, pyrosequencing, qPCR Rapid development and application of eDNA approaches Recent applications of eDNA have surprised some environmental managers because they seemed to emerge abruptly from the research phase (Darling & Mahon 2011). Yet the research that produced these tools illustrates typical and incremental scientific progress. The term 'environmental DNA' originates from microbiology (Ogram et al. 1987) and generally means DNA extracted from an environmental sample without isolating the target organism; for macrobiota, an entire organism is often not even present in the sample. Research targeting 'macrobial' eDNA began with detection of plant DNA in soil (Paget et al. 1998), with the first metagenetic approach (sensu Creer et al. 2010) to eDNA also applied to soil (Willerslev et al. 2003). The first application of macrobial eDNA analysis in an aquatic environment detected human, cow, pig and sheep DNA in river water (Martellini et al. 2005), with the first aquatic metagenetic approach aimed at riverine fishes (Minamoto et al. 2011). In this issue, Thomsen et al. (2012) apply the most current techniques, quantitative real-time PCR (qPCR) and next-generation sequencing, to demonstrate compellingly the power of the eDNA approach (Figs 1 and 2).
Specifically, results from qPCR provide an index of population size, which is a very important advance over PCR (Thomsen et al. 2012). In addition, qPCR has a lower detection threshold than traditional sampling tools, probably even lower than PCR because of the generally greater sensitivity of qPCR (Thomsen et al. 2012). Finally, using qPCR, Thomsen et al. (2012), expanding on other recent studies (Dejean et al. 2011), observed that the rapid degradation of eDNA in surface water means that the detection of eDNA indicates the very recent presence of aquatic species. In 80L tank experiments with a toad and a newt species, the longest that eDNA remained detectable at the highest organism density after removal of all amphibians was between 9 and 15 days (Thomsen et al. 2012).
The application by Thomsen et al. (2012) of next-generation sequencing shows how to move forward from targeted surveillance of one, or a handful of species, to more accurate estimates of species richness.
New eDNA tools in the toolbox to facilitate management goals for species and ecosystems Some prominent early applications of environmental DNA have involved the detection of faecal pollution (Martellini et al. 2005) and invasive species (Ficetola et al. 2008;Jerde et al. 2011). With invasive species, finding incipient populations early provides managers with options to act before a harmful species achieves high abundance (Robinson et al. 2011). Similarly, identifying and protecting habitats important to the persistence of biodiversity is daunting, particularly if threatened or endangered species are difficult to detect (Goldberg et al. 2011) or restrictions prevent sampling efforts that risk harm to individual organisms (Beja-Pereira et al. 2009). Thomsen et al. (2012) and other recent papers (Pfrender et al. 2010) point the way towards the power of eDNA for identifying habitats critical to protected species, and for assessing biodiversity for conservation, remediation and restoration efforts.
Trajectory of aquatic eDNA research: extracting more information more rapidly We believe that Thomsen et al. (2012) represents a macrobial eDNA research agenda that will proceed rapidly along at least two trajectories: species-specific population surveillance and monitoring; and metagenetic detection of multi- Fig. 1 The six species targeted in Thomsen et al. (2012)  ple species simultaneously. Massively parallel technologies like next-generation sequencing and microarrays can measure biodiversity across broad taxonomic scales. In comparison with microbial metagenetics, macrobial metagenetics benefits from a much smaller number of taxa, more reliable species boundaries and a considerable public database linking taxonomic and genetic identities. Macrobial metagenetics is already transforming the science of biodiversity assessment (Anderson-Carpenter et al. 2011).
However, one of the striking gaps in this rapidly growing field is the dearth of knowledge about how field and laboratory protocols influence the detection of eDNA (Goldberg et al. 2011), and how different environmental conditions affect the production, degradation and detection of eDNA. For example, a wide range of protocols have been reported for field sampling (e.g. number and volume of water samples), filtration (e.g. precipitation vs. various filters), DNA extraction (e.g. different kits and protocols), primer design and testing, PCR (e.g. number of reactions) and confirmation of species specificity (e.g. cloning, sequencing). The latter could be an underappreciated problem when it is critical to resolve among closely genetically related taxa that could have vastly different repercussions for management and ⁄ or biocontrol (Funk & Omland 2003). Experiments to systematically compare protocols are urgently needed. For laboratory protocols, adherence to the minimum information reporting guidelines for qPCR and metagenetics (Taylor et al. 2008) would at least make it more possible to compare protocols among publications even if the specific effects of different protocols were unknown.
Environmental DNA analysis is already an essential and influential tool in water quality monitoring, the early detection of invasive and other harmful species and the surveillance of imperilled species. With further refinements and comparisons of field and laboratory protocols, eDNA analysis will provide more information on taxonomic diversity and population abundance and find wider applications in environmental science research. Thomsen et al. (2012) give us confidence that: (i) eDNA analysis is applicable across broad taxonomic boundaries; (ii) the presence of eDNA indicates the recent presence of organisms; (iii) we can expect to learn more and more about population abundance with qPCR-based eDNA analysis; and (iv) nextgeneration sequencing of eDNA will yield increasingly accurate estimates of species richness. With eDNA, a lot can be learned from a cup of water. biodiversity including management of invasive species. SPE is interested in population genetics, evolutionary ecology and the application of genetics to species monitoring. JLF is interested in how populations adapt to the environment and the consequences of ecological specialization for speciation. ARM is interested in molecular ecology, phylogeography and methodological development for genetic monitoring in aquatic and marine ecosystems. MEP is interested in adaptation in response to environmental change and the interaction between evolution and community composition.