WWP2 regulates SIRT1‐STAT3 acetylation and phosphorylation involved in hypertensive angiopathy

Abstract WWP2 is a HECT‐type E3 ubiquitin ligase that regulates various physiological and pathological activities by binding to different substrates, but its function and regulatory mechanism in vascular smooth muscle cells (VSMCs) are still unknown. Here, we clarified the role of WWP2 in the regulation of SIRT1‐STAT3 and the impact of this regulatory process in VSMCs. We demonstrated that WWP2 expression was significantly increased in angiotensin II‐induced VSMCs model. Knockdown of WWP2 significantly inhibited angiotensin II‐induced VSMCs proliferation, migration and phenotypic transformation, whereas overexpression of WWP2 had opposite effects. In vivo experiments showed that vascular smooth muscle‐specific WWP2 knockout mice significantly relieved angiotensin II‐induced hypertensive angiopathy. Mechanistically, mass spectrometry and co‐immunoprecipitation assays identified that WWP2 is a novel interacting protein of SIRT1 and STAT3. Moreover, WWP2 formed a complex with SIRT1‐STAT3, inhibiting the interaction between SIRT1 and STAT3, then reducing the inhibitory effect of SIRT1 on STAT3, ensuing promoting STAT3‐K685 acetylation and STAT3‐Y705 phosphorylation in angiotensin II‐induced VSMCs and mice. In conclusion, WWP2 modulates hypertensive angiopathy by regulating SIRT1‐STAT3 and WWP2 suppression in VSMCs can alleviate hypertensive angiopathy vitro and vivo. These findings provide new insights into the treatment of hypertensive vascular diseases.

vascular diseases. Angiotensin II (AngII) binds to VSMCs' AngII type 1 receptor to activate STAT3 in order to initiate the proliferation and migration of VSMCs, representing key effects in vascular lesions caused by hypertension. [4][5][6] In addition, VSMCs include contractile and synthetic phenotypes according to structural and functional differences. The main function of contractile VSMCs is to contract and maintain the vascular wall tension, whereas synthetic VSMCs contain large amounts of organelles and a small number of muscle fibres, which can promote VSMCs proliferation and migration. VSMCs can be transformed from contractile to synthetic types under the action of AngII-induced STAT3 activation, which is the basis of VSMCs involvement in angiopathy caused by hypertension. [7][8][9] STAT3 activation mainly includes acetylation and phosphorylation of STAT3, which is regulated by deacetylase SIRT1. [10][11][12][13][14][15] deacetylates STAT3, ensuing inhibiting STAT3 phosphorylation, thus exerting its role in inhibiting AngII-induced VSMCs proliferation, migration and phenotypic transformation. [10][11][12][13][14][15] Therefore, SIRT1dependent STAT3 acetylation and phosphorylation are critical to VSMCs proliferation, migration and phenotypic transformation during hypertension condition. However, the regulatory mechanism among these events remains incompletely understood.
The E3 ubiquitin ligase WWP2 controls many biological processes such as the cell cycle, cell division, immune response, antigen presentation, apoptosis and cell signalling. [16][17][18][19] WWP2 not only degrades substrates, but also regulates genes post-transcriptionally. 20 Studies have demonstrated that WWP2 dysfunction can cause many diseases, including maxillofacial deformity, skeleton damage and embryonic dysplasia. 16,21,22 However, no study has assessed whether WWP2 is involved in the physiological and pathological processes of VSMCs.
Therefore, investigating the role and molecular mechanism of WWP2 in VSMCs phenotypic transformation as well as proliferation and migration may provide new insights into the treatment of hypertensive vascular diseases such as stroke, kidney and heart failure.
In this study, it was found for the first time that the expression of WWP2 is elevated in AngII-induced VSMCs proliferation, migration and phenotypic transformation model. In addition, WWP2 knockdown in VSMCs significantly relieved AngII-induced VSMCs proliferation, migration and phenotypic transformation, whereas WWP2 overexpression promoted this phenomenon. In vascular smooth muscle-specific WWP2 knockout mice, significantly relieved in AngII-induced hypertensive angiopathy was observed.
Therefore, In vivo and in vitro vascular smooth muscle-specific WWP2 knockout significantly alleviated hypertensive angiopathy.
Mechanistically, mass spectrometry and co-immunoprecipitation assays identified that STAT3 and SIRT1 were two novel proteins interacting with WWP2. Furthermore, WWP2 formed a complex with STAT3-SIRT1, competing for the interaction between SIRT1 and STAT3, then reducing the inhibitory effect of SIRT1 on STAT3, ensuing enhancing STAT3-K685 acetylation and STAT3-Y705 phosphorylation, which finally promoted AngII-induced proliferation, migration and phenotypic transformation in VSMCs and hypertensive angiopathy in mice.

| Micro-CT scanning and 3D reconstruction
Micro-computed tomography (micro-CT) on a micro-CT-Imaging SkyScan 1276 (Bruker, Germany) was carried out at 70 kV (200 µA), with 1237 projections (1520 × 1264) acquired within 6 minutes 43 seconds under continuous tube rotation. AngII signals were acquired in 20 × 20 × 20-μm 3 voxels with DataViewer (Bruker, Germany), with ring artefact correction. Then, the images were reconstructed and data visualized with NRecon (Bruker); CTAn (Bruker) was used for further evaluation. After 3D spine segmentation by interactively delineating the aorta in 100 and 200 slices (2 and 4 mm, respectively; Figure 6D,E), artery and vein circumferences were evaluated, with average cardiac tissue brightness after contrast agent injection into an artery and pre-contrast agent administration set at 100 and 0%, respectively. 25 The slices of each mouse were taken from the same position.

| Immunohistochemical (IHC) analysis
Aortic vascular tissue specimens from mice were fixed with 4% formalin (4 hours), paraffin embedded and sectioned at 5-µm, and the aortic slices of each mouse were taken from the same position.
After xylene dewaxing and rehydration by graded ethyl alcohol, the sections underwent haematoxylin and eosin (H&E) staining.

| Cell culture, cell transfections and coimmunoprecipitation
Human aortic vascular smooth muscle cells (HAVSMCs) were pro-  The resulting immuno-complexes were assessed by SDS-PAGE.

| HAVSMCs WWP2 knockdown methods and Plasmid construction
Control siRNAs and WWP2 siRNAs were provided by RIBOBIO (China).
Plasmids encoding the full-length human WWP2 (Shanghai Genechem) were cloned to HA-tagged destination vectors according to immunoprecipitation and immunoblotting needs.

Cell migration was assessed in transwell plates (Corning Life
Sciences, USA). A total of 5 × 10 3 cells were seeded into the upper chambers of 24-well plates. AngII treatment was used for 24 hours after starvation in serum-free medium for 24 hours. The top chambers were supplemented with serum-free H-DMEM, whereas the lower ones contained the complete medium with 10% FBS. Upon culture at 37°C overnight, cotton swabs were employed to remove non-migrating cells; those that migrated underwent fixation with chilled methanol and staining with crystal violet, and were counted in totally 5 random fields.
AngII treatment was used for 24 hours after starvation in serum-free medium for 24 hours, and Phalloidin (Molecular Probes, USA) staining of HAVSMCs was performed after fixation with 4% formalin (20 minutes) and permeabilization with 0.5% Triton X-100 (30 minutes), as directed by the manufacturer. Cell morphology and actin filaments were observed under a fluorescence microscope (Olympus).

| Western blot
Upon treatment, cell lysis was performed with co-immunoprecipitation buffer. The lysates were submitted to centrifugation (13 300 rpm;

| Statistical analysis
Data are mean ± standard deviation (SD). Homogeneity of variance was evaluated by the F test (group pair) or Brown-Forsythe test (multiple groups). The Shapiro-Wilk test was performed for assessing data normality. Student's t test and Welch t test were employed to assess data of group pairs with normal and skewed distributions, respectively (two groups). ANOVA and indicated non-parametric tests were performed to compare multiple groups. One-way ANOVA and two-way ANOVA were performed for comparing groups for single and two factors, respectively, and followed by Bonferroni post hoc testing. P values were adjusted for multiple comparisons when applicable. All data were analysed by SPSS 22.0 (SPSS, USA), and P < 0.05 was considered statistically significant.

| WWP2 interacts with STAT3 and promotes STAT3 acetylation and phosphorylation
STAT3 is an important regulator in VSMCs, and its acetylation and phosphorylation are critical to VSMCs proliferation, migration and phenotypic transformation. [4][5][6] Mass spectrometry and co-immunoprecipitation assays identified that the E3 ubiquitin ligase WWP2 was a novel protein interacting with STAT3. WWP2 was found to interact with STAT3 by internal and external co-immunoprecipitation assays ( Figure 1A,B).
As WWP2 was an E3 ubiquitin ligase, its effects on STAT3 expression were firstly detected, and the results showed that WWP2 did not regulate STAT3 expression (Supplementary Figure S1A,B).
However, co-immunoprecipitation assays results showed that WWP2 promoted the acetylation and phosphorylation levels of STAT3 ( Figure 1C,D), whereas its knockdown reduced the acetylation and phosphorylation levels of STAT3 ( Figure 1E,F).
The above results confirmed that WWP2, as a new interaction substrate of STAT3, promoted the acetylation and phosphorylation levels of STAT3, which may be the key for regulating VSMCs proliferation, migration and phenotypic transformation.

| WWP2 forms a complex with SIRT1-STAT3, competing for the interaction between SIRT1 and STAT3
As WWP2 is neither acetylase nor phosphokinase, we next explored the mechanism by which WWP2 promotes the acetylation and F I G U R E 1 WWP2 interacts with STAT3 and promotes STAT3 acetylation and phosphorylation. A, In HAVSMCs, endogenous coimmunoprecipitation was performed to assess the interaction between WWP2 and STAT3. B, As did exogenous HA-WWP2 and STAT3. C, HA-WWP2 was transfected into HAVSMCs. Pan-acetylation was isolated by co-immunoprecipitation, and STAT3 acetylation levels were assessed with anti-STAT3 antibody. D, HA-WWP2 was transfected into HAVSMCs. P-Tyr was isolated by co-immunoprecipitation, and STAT3 phosphorylation levels were assessed with anti-STAT3 antibody. E, WWP2 siRNA was transfected into HAVSMCs. Pan-acetylation was isolated by co-immunoprecipitation, and STAT3 acetylation levels were assessed with anti-STAT3 antibody. F, WWP2 siRNA was transfected into HAVSMCs. P-Tyr was isolated by co-immunoprecipitation, and STAT3 phosphorylation levels were assessed with anti-STAT3 antibody phosphorylation levels of STAT3. Mass spectrometry and co-immunoprecipitation assays results showed that SIRT1 was identified as another new physiological substrate of WWP2. Internal and external co-immunoprecipitation assays confirmed that WWP2 interacted with SIRT1 (Figure 2A,B). Moreover, the interaction between WWP2 and SIRT1 was enhanced after stimulation by AngII in VSMCs ( Figure 2C,D).
The above results revealed that WWP2 competed with the interaction between SIRT1 and STAT3, ensuing reducing SIRT1-regulated deacetylation and dephosphorylation of STAT3, which promotes the acetylation and phosphorylation levels of STAT3.

| WWP2 antagonizes SIRT1-inhibited STAT3-K685 acetylation and STAT3-Y705 phosphorylation in AngII-induced VSMCs
Previous studies have shown that SIRT1 inhibits STAT3 acetylation and phosphorylation mainly acting on STAT3-K685 and STAT3-Y705, respectively. 15 Therefore, in order to provide the most direct and important evidence that WWP2 promotes STAT3 acetylation and phosphorylation by inhibiting the effect of SIRT1 on STAT3, we further clarify the loci in which WWP2 promotes STAT3 acetylation and phosphorylation. In this study, STAT3 acetylation and phosphorylation locus antibodies were used for co-immunoprecipitation assays after WWP2 overexpression and knockdown in VSMCs, respectively. The results showed that WWP2 overexpression increased STAT3-K685 acetylation ( Figure 3C) and STAT3-Y705 phosphorylation ( Figure 3A).

| WWP2 promotes AngII-induced VSMCs proliferation and migration, and up-regulates PCNA, MMP2 and MMP9 expression
STAT3 acetylation and phosphorylation are critical to VSMCs proliferation, migration and phenotypic transformation. 4-6 Therefore, the F I G U R E 2 WWP2 forms a complex with SIRT1-STAT3, competing for the interaction between SIRT1 and STAT3. A and B, In HAVSMCs, endogenous coimmunoprecipitation was performed to assess the interaction between WWP2 and SIRT1. B, As did exogenous HA-WWP2 and SIRT1. C and D, Endogenous co-immunoprecipitation was performed to assess the interaction between WWP2 and SIRT1 with the addition of 10 −6 mol/L angiotensin II. E, HA-WWP2 was transfected into HAVSMCs. Endogenous co-immunoprecipitation between SIRT1 and STAT3 was assessed. F, WWP2 siRNA was transfected into HAVSMCs. Endogenous co-immunoprecipitation between SIRT1 and STAT3 was assessed  Figure 4K-N).
The above results showed that WWP2 promoted AngII-induced VSMCs proliferation and migration, and its knockdown in VSMCs might become an important tool for suppressing hypertensive VSMCs proliferation and migration.

| WWP2 promotes AngII-induced VSMCs phenotypic transformation and down-regulates the contractile phenotypic proteins α-SM-actin and SM22α
Next, this study assessed the effects of WWP2 on AngII-induced Taken altogether, these findings indicated that WWP2 formed a complex with SIRT1-STAT3, inhibiting the interaction between SIRT1 and STAT3, then reducing the inhibitory effect of SIRT1 on STAT3, ensuing promoting STAT3-K685 acetylation and STAT3-Y705 phosphorylation, which finally promoted AngII-induced VSMCs proliferation and migration as well as phenotypic transformation, and WWP2 silencing in VSMCs may become a critical tool in controlling vascular F I G U R E 3 WWP2 promotes levels of STAT3-K685 acetylation and STAT3-Y705 phosphorylation. A and B, HA-WWP2 and WWP2 siRNA were transfected into HAVSMCs. STAT3 was isolated by co-immunoprecipitation, and STAT3-Y705 phosphorylation levels were assessed with anti-STAT3-Y705 antibody. C and D, HA-WWP2 and WWP2 siRNA were transfected into HAVSMCs. STAT3 was isolated by co-immunoprecipitation, and STAT3-K685 acetylation levels were assessed with anti-STAT3-K685 antibody. E and F, HA-WWP2 and WWP2 siRNA were transfected into HAVSMCs with or without treatment of 10 −6 mol/L AngII after serum-free medium starvation. STAT3-K685 acetylation and STAT3-Y705 phosphorylation levels were assessed with anti-STAT3-K685 and anti-STAT3-Y705 antibodies F I G U R E 4 WWP2 promotes AngII-induced HAVSMCs proliferation and migration, and up-regulates the expression of PCNA, MMP2 and MMP9. A, HAVSMCs were transfected with the HA-control or HA-WWP2 plasmid for 36 h and treated with 0, 10 −8 , 10 −7 and 10 −6 mol/L AngII for 24 h after serum-free medium starvation, respectively. CCK8 was used to assess HAVSMCs viability, and quantitated data were shown as means ± SD (***P < .001, unpaired Student's t test). D, As did HAVSMCs were transfected with the control siRNA or WWP2 siRNA. B and C, HAVSMCs were transfected with the HA-control or HA-WWP2 plasmid for 36 h and treated with or without 10 −6 mol/L AngII for 24 h after serum-free medium starvation. Transwell was used to assess HAVSMCs migration, and quantitated data were shown as means ± SD (***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing). Scale bar 100 µm. E and F, As did HAVSMCs were transfected with the control siRNA or WWP2 siRNA. G-J, HAVSMCs were transfected with the HA-control or HA-WWP2 plasmid for 36 h and treated with or without 10 −6 mol/L AngII for 24 h after serum-free medium starvation. PCNA, MMP2 and MMP9 were detected by Western blot, and quantitated data were shown as means ± SD (***P < .001, unpaired Student's t test; # P < .05, ### P < .001, two-way ANOVA with Bonferroni post hoc testing). K-N, As did HAVSMCs were transfected with the control siRNA or WWP2 siRNA. All the experiments were repeated three times | 9049 diseases associated with AngII-induced VSMCs proliferation, migration and phenotypic transformation, such as hypertensive angiopathy-hypertensive vascular thickening.

| Mice vascular smooth muscle-specific WWP2 knockout reduces STAT3-K685 acetylation and STAT3-Y705 phosphorylation, and relieves hypertensive arteries and veins angiopathy
To assess the effects and mechanisms of WWP2 on AngII-induced

| D ISCUSS I ON
The following important new findings were obtained in this study: F I G U R E 5 WWP2 promotes AngII-induced HAVSMCs phenotypic transformation and decreases the expression of α-SM-actin and SM22α proteins. A, HAVSMCs were transfected with the HA-control or HA-WWP2 plasmid for 36 h and treated with or without 10 −6 mol/L AngII for 24 h after serum-free medium starvation. Phalloidine dye was used to assess HAVSMCs phenotypic transformation and intracellular myofilaments were labelled with green fluorescence. Quantitated data were shown as means ± SD (***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing). Scale bar 50 µm. B, As did HAVSMCs were transfected with the control siRNA or WWP2 siRNA. C and D, HAVSMCs were transfected with the HA-control or HA-WWP2 plasmid for 36 h and treated with or without 10 −6 mol/L AngII for 24 h after serum-free medium starvation. α-SM-actin and SM22α were detected by Western blot, and quantitated data were shown as means ± SD (**P < .01, ***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing). E and F, As did HAVSMCs were transfected with the control siRNA or WWP2 siRNA. All the experiments were repeated three times F I G U R E 6 Mice vascular smooth muscle-specific WWP2 knockout reduces STAT3-K685 acetylation and STAT3-Y705 phosphorylation, and relieves hypertensive arteries and veins angiopathy. A, Immunofluorescence of blood vessel tissues was performed to assess WWP2 expression levels in SM22α Cre-;WWP2 FL/FL and SM22α Cre-;WWP2 FL/FL mice. Scale bar 50 µm. B, Total protein was obtained from blood vessel tissues of SM22α Cre-;WWP2 FL/FL and SM22α Cre-;WWP2 FL/FL mice. Western blot analyses were performed to assess WWP2 expression levels, and quantitated data were shown as means ± SD (each group of mice, n = 9; ***P < .001, unpaired Student's t test). C, Construction strategy of gene conditional elimination targeting vector. D and E, Aortic blood vessel detection by micro-CT after administration of blood pool contrast solution containing iodine (eXIA 160XL), which allows a spatial resolution of 20 μm voxels 2D crosssectional images in SM22α Cre-;WWP2 FL/FL and SM22α Cre-;WWP2 FL/FL mice delineating from the heart pre-contrast agent injection (0%) to the aorta boundaries in 100 slices and 200 slices. Scale bar 4 mm. F and G, Vein circumference and artery circumference were determined by in vivo micro-CT, and the slices of each mouse were taken from the same position. Quantitated data were shown as means ± SD (each group of mice, n = 9; **P < .01, ***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing). H and I, Western blot was carried out to assess STAT3-Y703 and STAT3-K685 expression levels. Quantitated data were shown as means ± SD (each group of mice, n = 9; ***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing) WWP2 is an E3 ubiquitin ligase of the HECT-type NEDD4 family, which participates in the regulation of various physiological and pathological processes by interacting with different substrates. [16][17][18][19][20][21][22] Initially, WWP2 was considered a tumour-promoting factor involved in the development of tumours by regulating PETN and Smad. 26,27 Currently, WWP2 is considered to be involved in various life activities, such as development and ossification. 16,[19][20][21][22] In previous studies, WWP2 was discovered to be highly expressed in diabetic cardiomyopathic heart. 28 In primary cardiac fibroblasts, TGFβ1 irritates the N-terminal subtype of WWP2 to enter the nucleus, subsequently strengthening the activity of WWP2-FL to promote the combining with Smad2, and may promote its monoubiquitination, thus activating the downstream Pro-fibrogenic gene programme. 29,30 Therefore, WWP2 plays an important role in controlling pathological myocardial fibrosis and heart failure and improving the clinical prognosis of F I G U R E 7 Mice vascular smooth muscle WWP2 knockout significantly relieves AngII-induced hypertensive vascular thickening and systolic blood pressure. A and B, SM22α Cre-;WWP2 FL/FL and SM22α Cre-;WWP2 FL/FL mice induced by AngII or Nacl for 14 days. HE staining was carried out to assess aortic blood vessel thickness from SM22α Cre-;WWP2 FL/FL and SM22α Cre-;WWP2 FL/FL mice, and the aortic slices of each mouse were taken from the same position. Scale bar 100µm. Quantitated data were shown as means ± SD (each group of mice, n = 9; ***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing). C and D, Systolic and diastolic blood pressures are measured, and quantitated data were shown as means ± SD (each group of mice, n = 9; ***P < .001, unpaired Student's t test; # P < .05, two-way ANOVA with Bonferroni post hoc testing). E and F, Total protein was obtained from aortic vascular tissue of SM22α Cre-;WWP2 FL/FL and SM22α Cre-;WWP2 FL/FL mice, and Western blot was carried out to assess the expression levels of PCNA, MMP2 and MMP9. Quantitated data were shown as means ± SD (each group of mice, n = 9; ***P < .001, unpaired Student's t test; ### P < .001, two-way ANOVA with Bonferroni post hoc testing) patients with heart disease. In our previous study, WWP2 interacts with the BRCT domain of PARP1 and ubiquitinates its K249 and K418. WWP2 cannot promote the ubiquitination levels of PARP1 in MycCre + WWP2 FL/FL mice induced by isoprenaline (ISO) and the expression levels of PARP1 increases, thus promoting ISO triggered cardiac remodelling. Therefore, WWP2 can degrade PARP1 and protect it from ISO triggered cardiac remodelling. This provides a basis for the study of the treatment of heart remodelling-related diseases. 31 In addition, our another previous study showed that the silencing of endothelial/ myeloid-specific WWP2 gene markedly increased the endothelial injury and vascular remodelling induced by Ang II. WWP2 promotes the degradation of lysine residue 174 (K174)-Septin4 through ubiquitin proteasome, thus inhibiting the formation of Septin4-PARP1 endothelial damage complex. 32 Therefore, WWP2-Septin4 pathway may be a new target for the treatment of atherosclerosis and hypertension. 33 In this study, in vivo and in vitro experiments revealed key roles for WWP2 in VSMCs proliferation, migration and phenotypic transformation, as well as hypertensive angiopathy. Furthermore, it was found that the E3 ubiquitin ligase WWP2 played a new non-ubiquitination function, that is competitive binding to SIRT1 and STAT3, antagonizing STAT3 deacetylation by SIRT1, ensuing promoting STAT3 acetylation, thus enhancing STAT3 phosphorylation. In conclusion, WWP2 has not been served as a therapeutic target; however, it is an important potential target for targeted treatment of various cardiovascular-related diseases.
STAT3 is an important member of the family of kinase signal transduction and transcriptional activators. It participates in a series of cardiovascular diseases such as hypertension, atherosclerosis, myocardial infarction and ischaemia-reperfusion injury by regulating biological events such as chronic inflammation, oxidative stress, cell proliferation and apoptosis. 34-37 STAT3 and IL-6 double-knockout mice significantly reduce AngII-induced STAT3 phosphorylation, which counteracts the occurrence of AngII-induced hypertensive angiopathy. 5,6 Additionally, the atherosclerotic area in the aortic root of oxidized phospholipid-mediated STAT3 −/− mice decreases significantly compared with that of STAT3 +/+ mice. 4 Meanwhile, STAT3 knockout in VSMCs significantly reduces AngII-induced VSMCs proliferation and migration. 38,39 Moreover, studies have demonstrated that STAT3 promotes VSMCs proliferation and migration mainly depends on the phosphorylation activity, and such phosphorylation activity is regulated by STAT3 acetylation. 15,40 In this study, it was found for the first time that STAT3 phosphorylation and acetylation were regulated by WWP2, with the regulatory loci including STAT3-K685 and STAT3-Y705, which further confirmed the roles of WWP2 in VSMCs proliferation and migration, as well as hypertensive angiopathy.
We found that SIRT1 was another new protein interacting with WWP2, by exploring the specific mechanism by which WWP2 regulates STAT3 acetylation and phosphorylation. SIRT1 is a member of the mammalian family of Sirtuins and a nicotinamide adenine dinucleotide (NAD + )-dependent deacetylase. 10,15 Studies have shown that SIRT1 deacetylates STAT3, ensuing inhibiting STAT3 acetylation and phosphorylation. 14,15 In addition, SIRT1 plays an important protective role in the cardiovascular system through its functions on STAT3 deacetylation. [10][11][12][13][14][15] A study revealed that atherosclerosis in SIRT1 +/-Apo E −/− mice is decreased significantly compared with ApoE −/− counterparts. 10 SIRT1 can also suppress AngII-induced VSMCs proliferation and migration as well as phenotypic transformation, counteracting hypertensive angiopathy. [11][12][13] In this study, WWP2 was identified as a new protein interacting with SIRT1.
WWP2 formed a complex with SIRT1-STAT3 to complete with the interaction between SIRT1 and STAT3, ensuing antagonizing STAT3 deacetylation by SIRT1. This resulted in enhanced STAT3-K685 acetylation and STAT3-Y705 phosphorylation, ensuing promoting VSMCs proliferation, migration and phenotypic transformation.
In this study, WWP2 was found to have important regulatory roles in AngII-induced VSMCs proliferation, migration and phenotypic transformation in vitro (WWP2 knockdown/overexpression in VSMCs), as well as hypertensive angiopathy in vivo (vascular smooth muscle-specific WWP2 knockout mice). Moreover, this study revealed that WWP2 promoted STAT3-K685 acetylation and STAT3-Y705 phosphorylation by antagonizing the modification effect of SIRT1 on STAT3. These findings suggested a new regulatory tool for counteracting AngII-induced VSMCs proliferation, migration and phenotypic transformation as well as hypertensive angiopathy, providing novel insights into the treatment of hypertensive vascular diseases such as stroke, and heart and kidney failure. It is of great significance to further investigate whether other E3 ubiquitinated ligases are involved in hypertensive angiopathy. In addition, the roles of WWP2 in other cardiovascular diseases and whether WWP2 has functions in patients with hypertensive angiopathy should be further assessed.
In conclusion, WWP2 formed a complex with SIRT1-STAT3, inhibiting the interaction between SIRT1 and STAT3, then reducing the inhibitory effect of SIRT1 on STAT3, ensuing enhancing STAT3-K685 acetylation and STAT3-Y705 phosphorylation, which finally promoted AngII-induced proliferation, migration and phenotypic transformation in VSMCs and hypertensive angiopathy in mice.
In vivo and in vitro vascular smooth muscle-specific WWP2 knockout alleviates hypertensive angiopathy. This study provides new insights into the treatment of hypertensive vascular diseases such as stroke, and heart and kidney failure.

ACK N OWLED G EM ENTS
This work was financially supported by National Natural Science