We developed a method to isolate planarian viable cells for single-cell transcriptome analysis. The sorted cells by our method demonstrated high cell viability and high quality in single-cell RNA-seq library construction.

A series of truncation analyses of fission yeast Pkd2 revealed that Pkd2 localizes to the ER via transmembrane domains and regulates the cytoplasmic calcium signaling. Human Pkd2 localized to the ER and has some effect on activation of cytoplasmic calcium signaling in fission yeast; however, human PKD2 could not rescue the loss of pkd2 in fission yeast.

Yeast eIF4E-binding protein Caf20 is phosphorylated by Tor complex 1 (TORC1). Caf20 may regulate translation initiation via TORC1.

The behaviors of cells, tissues, and organs are controlled by the extracellular environment in addition to their autonomous regulatory system. Lumicrine signaling is a transluminal secreted signal transduction in the male reproductive tract lumen through which sperm functional maturation is regulated.

Wnt10b, a crucial member of the Wnt gene family, is involved in many physiological processes. However, the impact of Wnt10b knockdown on the function of brown adipose tissue is yet largely unknown. This study indicates that Wnt10b knockdown enhances UCP1 expression and inhibits the adipogenic differentiation of brown adipocytes, providing a novel option for therapeutic interventions in adiposity.