As the official journal of Japanese Society of Developmental Biologists, Development, Growth & Differentiation publishes multidisciplinary research dealing with developmental phenomena in animals, plants and microorganisms since 1969.

We welcome papers dealing with molecular, genetic, cellular, and organismal phenomena including metamorphosis and regeneration while using experimental, theoretical, and bioinformatic approaches. Papers on other related fields are also welcome, such as stem cell biology, genomics, neuroscience, Evodevo, Ecodevo, and basic medical science.

Articles

Virtual Special Issue: Methods & Protocols
December 2022

Issue edited by:
H. Ogino, Y. Kamei, T. Hayashi, J. Sakamoto, M. Suzuki, T. Igawa, M. Kondo, M. Taira


Virtual Special Issue: Pluripotent Stem Cells in Developmental Biology
April 2021

Issue edited by:
M. Ikeya, Y. Toyooka, M. Eiraku


Visit DGD Special Issue Archive here


Open access

An analysis of semaphorin-mediated cellular interactions in the Caenorhabditis elegans epidermis using the IR-LEGO single-cell gene induction system

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To elucidate how the intercellular signaling mediated by semaphorin controls the position of the border between epidermal cells of C. elegans, we performed mosaic gene expression analyses by using IR-LEGO. Our result suggests that semaphorin promotes the extension of a certain cell. This is in contrast to the previous finding that semaphorin mediates cell retraction in a different group of C. elegans epidermal cells, providing another example of the context-dependent regulation of cell shape by the semaphorin signaling system.

Open access

Assembly of continuous high-resolution draft genome sequence of Hemicentrotus pulcherrimus using long-read sequencing

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Assembly of the continuous high-resolution draft genome sequence of sea urchin, Hemicentrotus pulcherrimus, was performed using long-read sequencing. Two long tandem repeats of early histone genes loci, various cis-regulatory repeat sequences, and 185 of the homologous sequences of Ars-insulator core region were identified. These results provide further advances for genome-wide research of development, gene regulation, and intranuclear structural dynamics of multicellular organisms using H. pulcherrimus.

Epithelial–mesenchymal transition contrast in the amputated tail and limb of the northern house gecko, Hemidactylus flaviviridis

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The current study focuses on epithelial–mesenchymal transition (EMT) contrast in the amputated tail and limb of northern house geckos. We observed scar-free healing followed by activation of EMT in the autotomized tail, thus triggering regeneration. In contrast, the amputated limb showed no signs of EMT, possibly due to collagen deposition at the intersection between wound epithelium and mesenchyme.

Comparative transcriptomic characterization of the ovary in the spawning process of the mud crab Scylla paramamosain

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Spawning is a physiological process that is fundamental for the reproduction of Scylla paramamosain. Our results suggest that octopamine (OA) and tyramine (TA) could induce oviposition, while dopamine (DA) and serotonin (5-hydroxytryptamine [5-HT]) inhibit oviposition. The 20-hydroxyecdysone (20E) and methyl farnesoate (MF) signal pathways might be positively associated with oviposition. Furthermore, numerous transcripts that encode neuropeptides and their G-protein-coupled receptors (GPCRs), such as CNMamide, RYamide, ecdysis-triggering hormone (ETH), GPA2/GPB5 receptor, and Moody receptor, appear to be differentially expressed during the spawning process. Our work is the first spawning-related investigation of S. paramamosain focusing on the ovary at the whole transcriptome level.

Open access

Genomic screening of fish‐specific genes in gnathostomes and their functions in fin development

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In this study, we comprehensively searched for fish-specific genes in gnathostomes that contribute to development of the fin, a fish-specific trait. We suggest that the strategy of identifying animal group-specific genes is functional and useful. The methods applied here could be used in future studies to identify trait-associated genes in other animal groups.

Open access

Development of a heat-stable alkaline phosphatase reporter system for cis-regulatory analysis and its application to 3D digital imaging of Xenopus embryonic tissues

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In this study, we (1) optimized the conditions for chromogenic detection of placental alkaline phosphatase (PLAP) (alkaline phosphatase, placental type) expression for use as a transgenic reporter in Xenopus embryos, (2) investigated the sensitivity and responsiveness of PLAP to the cis-regulatory elements of various genes, and (3) analyzed embryos expressing PLAP in specific tissues using the correlative light microscopy and block-face imaging (CoMBI) method. The results of these experiments demonstrate the efficacy of the PLAP reporter system for detecting enhancer/promoter activities driving deep tissue expression and its combination with the CoMBI method as a powerful approach for 3D digital imaging analysis of specific tissue/organ structures in opaque Xenopus embryos.

Open access

Cell adhesion and actin dynamics factors promote axonal extension and synapse formation in transplanted Drosophila photoreceptor cells

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Presynapse formation in axons elongating into the lamina was observed upon overexpression of Ncad and Tsr.

Open access

Evolutionarily conserved roles of foxg1a in the developing subpallium of zebrafish embryos

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A zebrafish foxg1a mutant line was established and the phenotypes were analyzed, showing the pivotal role of foxg1a in directing the dorsoventral patterning of the telencephalon and its necessity in preserving neural progenitors. Collectively, these findings underscore evolutionarily conserved functions of foxg1 in the development of the subpallium in vertebrate embryos.

Open access

FGF‐stimulated tendon cells embrace a chondrogenic fate with BMP7 in newt tissue culture

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A serum-free tissue culture system for tendons with regeneration-related factors was established to explore cell dynamics during joint regeneration in newts. Our findings suggest that FGF-stimulated cells from tendons may aid in joint cartilage regeneration during functional elbow joint regeneration in newts.

Open access

Dissection of N-deacetylase and N-sulfotransferase activities of NDST1 and their effects on Wnt8 distribution and signaling in Xenopus embryos

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Heparan sulfate (HS) chains are important modulators of Wnt proteins in the extracellular space. The bifunctional HS modification enzyme N-deacetylase/N-sulfotransferase 1 (NDST1) has a positive effect on Wnt signaling; however, which activity is actually involved in Wnt signaling remains unclear. In the present work, we dissected these activities by introducing mutations into NDST1.

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