• Issue

    Cell Proliferation: Volume 57, Issue 2

    February 2024

FEATURED COVER

Open Access

Featured Cover

  • First Published: 07 February 2024
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The cover image is based on the Original Article Rapid and efficient generation of a transplantable population of functional retinal ganglion cells from fibroblasts by Zihui Xu et al., https://doi.org/10.1111/cpr.13550.

ADDITIONAL COVER

Open Access

Additional Cover

  • First Published: 07 February 2024
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The cover image is based on the Original Article Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death by Georg Csukovich et al., https://doi.org/10.1111/cpr.13544. Image Credit: Georg Csukovich.

ISSUE INFORMATION

Open Access

Issue Information

  • First Published: 07 February 2024

REVIEW

Open Access

Notochordal cells: A potential therapeutic option for intervertebral disc degeneration

  • First Published: 11 September 2023
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The biological process of NCs development into NPCs and its cell structure changes.

ORIGINAL ARTICLES

Open Access

NELFA and BCL2 induce the 2C-like state in mouse embryonic stem cells in a chemically defined medium

  • First Published: 17 August 2023
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In mouse embryonic stem cells (ESCs), a minority of cell population displays totipotent features resembling 2-cell-stage embryos, known as 2-cell-like (2C-like) cells. However, how ESCs transit into the 2C-like state remains largely unknown. Here, we report that overexpression of maternal factor-negative elongation factor A (Nelfa) increases the conversion of the 2C-like state in ESCs in chemically defined condition while deletion of endogenous Nelfa did not block the transition of 2C-like state. We also demonstrate that overexpression of Nelfa significantly increases the efficiency of somatic cell reprogramming. Interestingly, overexpression of Nelfa combined with Bcl2 robus activates the 2C-like state in ESCs which exhibit the ability of bi-potential cell fate. We further demonstrate that overexpression of Bcl2 increases endogenous Nelfa expression and can successfully induce the 2C-like state of ESCs even in the absence of Nelfa. Our findings highlight an important role of BCL2 in 2C-like state and provide insights into the mechanism underlying Nelfa and Bcl2 mediated totipotent state in mouse ESCs.

Open Access

The efficient generation of functional human hepatocytes from chemically induced pluripotent stem cells

  • First Published: 09 October 2023
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A new differentiation protocol mimicking the two-stage development of hepatoblasts to potentiate their transition to hepatic progenitor cells (hiHPCs), which permits the high-efficiency generation of hiHPCs from chemically induced pluripotent stem cells (hCiPSCs).

Open Access

Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine-derived stem cells

  • First Published: 18 September 2023
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Schematic illustration of the study design. In vitro, the effects of SIS and U-SIS on the behaviour of chondrocyte: cell proliferation, migration, chondrogenic ability, regulation of protein secretion and changes in genes and signal pathway protein related to cartilage phenotype. In vivo, perichondrium, SIS and U-SIS wrapped diced cartilage were used for grafting and their effect on cartilage repair was evaluated in a rabbit ear cartilage defect model.

Open Access

Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death

  • First Published: 11 September 2023
Description unavailable

Basal out organoid polarity can easily be reversed by extracellular matrix removal to generate fully apical out intestinal organoids. However, this polarity reversal is accompanied by decreased proliferation coupled with increased cell death, ultimately leading to the death of apical out organoids, questioning their applicability and usefulness for disease modelling. Despite these differences, we demonstrate that transcript expression of key intestinal marker genes is unaffected by polarity reversal.

Open Access

Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation

  • First Published: 13 September 2023
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Alternative splicing (AS) disruption has been linked to disorders of muscle development, as well as muscular atrophy. However, the precise changes in AS patterns that occur during myogenesis are not well understood. We employed isoform long-reads RNA-seq (Iso-seq) and single-cell RNA-seq (scRNA-seq) to investigate the AS landscape during myogenesis. Our Iso-seq data identified 61,146 full-length isoforms representing 11,682 expressed genes, of which over 52% were novel. We identified 38,022 AS events, with most of these events altering coding sequences and exhibiting stage-specific splicing patterns. We identified AS dynamics in different types of muscle cells through scRNA-seq analysis, revealing genes essential for the contractile muscle system and cytoskeleton that undergo differential splicing across cell types. Gene-splicing analysis demonstrated that AS acts as a regulator, independent of changes in overall gene expression. Two isoforms of splicing factor TRA2B play distinct roles in myogenic differentiation by triggering AS of TGFBR2 to regulate canonical TGF-β signalling cascades differently. Our study provides a valuable transcriptome resource for myogenesis and reveals the complexity of AS and its regulation during myogenesis.

Open Access

AFF4 regulates osteogenic potential of human periodontal ligament stem cells via mTOR-ULK1-autophagy axis

  • First Published: 20 September 2023
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The present study shows that AFF4 protein expression was significantly lower in periodontitis tissues than healthy periodontal tissues. And we further revealed that AFF4 regulates osteogenic differentiation of hPDLSCs. The above findings provide a clue for possible engagement of AFF4 in local dyshomeostasis in periodontitis.

Open Access

Apoptotic vesicles derived from human red blood cells promote bone regeneration via carbonic anhydrase 1

  • First Published: 11 September 2023
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Apoptotic vesicles derived from human red blood cells promoted the osteogenesis of human mesenchymal stem cells and enhanced bone regeneration via carbonic anhydrase I. The figure was created with BioRender.com.

Open Access

Pyruvate kinase M2 regulates kidney fibrosis through pericyte glycolysis during the progression from acute kidney injury to chronic kidney disease

  • First Published: 25 September 2023
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Schematic diagram depicting proposed mechanisms of PKM2-induced renal pericyte-myofibroblast transdifferentiation through regulation of transcription levels of downstream LDHA and GLUT1 and lactate production. GLUT1, glucose transporter 1; LDHA, lactate dehydrogenase A; PKM2, pyruvate kinase M2; TGFβ1, transforming growth factor β1.

Open Access

Rapid and efficient generation of a transplantable population of functional retinal ganglion cells from fibroblasts

  • First Published: 23 September 2023
Description unavailable

The transcription factor (TF) combination ASCL1 + BRN3B + PAX6 could efficiently reprogram mouse embryonic fibroblasts (MEFs) into induced retinal ganglion cells (iRGCs). These iRGCs not only expressed several molecular markers of native RGCs, but also exhibited typical RGC electrophysiological properties. Upon transplantation into mouse retinal explants or NMDA-damaged retinas, iRGCs could integrate into the ganglion cell layer, survive for a prolonged period, grow long neuron fibres, form synapses with host cells, and exhibit electrophysiological functions.

Open Access

Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120

  • First Published: 24 September 2023
Description unavailable

This study demonstrates that omega-3 effectively protects spermatogonia from the effects of chemotherapy and oxidation; specifically, it rescues spermatogonia function by enhancing bone morphogenic protein 4 (BMP4) expression in Sertoli cells. We further found that some eicosapentaenoic acid metabolites, including 12-hydroxyeicosapentaenoic acid (12-HEPE), were produced at high levels in the testes of mice administered omega-3 and that 12-HEPE specifically induced BMP4 expression through the GPR120-ERK1/2 signalling pathway in Sertoli cells.