• Issue
    Volume 28, Issue 8
    August 2023


Free Access

Issue Information

  • Pages: 535-538
  • First Published: 01 August 2023
Description unavailable

Front cover: Morning glories bloom on an August morning. The pattern of the fl owers is reminiscent of paraspeckles, which are nonmembranous organelles formed by lncRNA Neat1. Also, the tendril extending right-upwards looks like Hero proteins, which are intrinsically disordered. A review article on nondomain biopolymers, including Neat1 and Hero proteins, appears in this issue of this journal (Arakawa et al. (2023) Genes Cells 28: 539-552, DOI: 10.1111/gtc.13050).


Open Access

Nondomain biopolymers: Flexible molecular strategies to acquire biological functions

  • Pages: 539-552
  • First Published: 30 May 2023
Description unavailable

This review challenges the long-held belief in molecular biology that the functionality of biomolecules solely hinges on conserved sequences and their resultant structures. Instead, we explore the roles and molecular mechanisms of “nondomain biomolecules,” which lack conserved functional domains and utilize inherent flexibility for their functional roles. Our hypothesis suggests that both domain and nondomain biopolymers cooperate to facilitate flexible and efficient molecular processes in the highly crowded intracellular milieu.


Quick and affordable DNA cloning by reconstitution of Seamless Ligation Cloning Extract using defined factors

  • Pages: 553-562
  • First Published: 03 May 2023
Description unavailable

Molecular mechanisms underlying the widely accepted Seamless Ligation Cloning Extract method were elucidated. A novel protocol was developed for quick and affordable seamless DNA cloning. Commercial kits will be replaced by the presented method.

Squamous cell carcinoma-derived G-CSF promotes tumor growth and metastasis in mice through neutrophil recruitment and tumor cell proliferation, associated with poor prognosis of the patients

  • Pages: 573-584
  • First Published: 29 May 2023
Description unavailable

High expression of G-CSF in the tumor tissues of esophageal squamous cell carcinoma (ESCC) patients correlated with poor prognosis. Deletion of G-CSF in murine tumor cells mitigated tumor growth and metastasis to lymph node and lung of subcutaneous NR-S1M tumors in the mice. Mechanistically, G-CSF facilitates tumor progression in mice through bi-functional effects to promote neutrophil recruitment and tumor cell proliferation, which may render poor prognosis to the ESCC patients with high G-CSF expression.

Transcriptional regulation of adipogenin expression in liver steatosis by hepatic peroxisome proliferator-activated receptor gamma

  • Pages: 585-594
  • First Published: 30 May 2023
Description unavailable

  • Adig is highly expressed in the fatty liver of ob/ob and other mice models.
  • ADIG expression is reduced upon liver-specific PPARγ knockout in ob/ob mice.
  • PPARγ enhances Adig transcription by binding to the PPARγ-responsive element.

Meiotic DNA double-strand break-independent role of protein phosphatase 4 in Hop1 assembly to promote meiotic chromosome axis formation in budding yeast

  • Pages: 595-614
  • First Published: 27 May 2023
Description unavailable

Meiosis-specific chromosomal axis-loop structures are important for meiotic recombination reactions and control to ensure accurate chromosome segregation. Here, we showed that protein phosphatase 4 (PP4) is required to promote the assembly of a chromosomal axis component Hop1 and Red1 onto meiotic chromatin via direct interaction with Hop1 in budding yeast. These results indicate phosphorylation-mediated regulation of Hop1 recruitment onto chromatin during chromosome axis construction before meiotic double-strand break formation.


Serine-rich domain of RNPS1 functions in activation of alternative splicing

  • Pages: 615-623
  • First Published: 19 May 2023
Description unavailable

This study uncovered that RNPS1 is involved in the alternative splice site activation of the HIV-1 substrate and endogenous apoptotic transcripts. We show that the serine-rich domain is required for exon inclusion of the HIV-1 based pre-mRNA and apoptotic genes.