• Issue

    Plant, Cell & Environment: Volume 45, Issue 2

    275-590
    February 2022

ISSUE INFORMATION

Free Access

Issue Information

  • Pages: 275-278
  • First Published: 19 January 2022

ORIGINAL ARTICLES

Metabolism-mediated mechanisms underpin the differential stomatal speediness regulation among ferns and angiosperms

  • Pages: 296-311
  • First Published: 20 November 2021
Summary Statement

The slower stomatal closure in ferns is associated to the lack of abscisic acid-responsiveness, to a reduced capacity to respond to mesophyll-derived sucrose and to a higher carbon allocation toward secondary metabolism, as compared to angiosperms.

ZmERF21 directly regulates hormone signaling and stress-responsive gene expression to influence drought tolerance in maize seedlings

  • Pages: 312-328
  • First Published: 06 December 2021
Summary Statement

Drought stress adversely destroys maize development during the whole life cycle, finally reducing its yield and quality. Thus, improving the performance of maize against drought tolerance is a priority of breeding programs. Here, we identified a novel transcription factor ZmERF21 that is tightly associated with drought tolerance in maize seedlings. ZmERF21 is mainly expressed in root and leaf. Mechanistic analysis demonstrated that ZmERF21 could directly bind to the genes involved in hormone (ethylene, abscisic acid) and Ca signaling pathways. Furthermore, overexpression of ZmERF21 in maize significantly increased drought tolerance, evidenced by chlorophyll content and the activities of antioxidant enzymes. Taken together, our results revealed that ZmERF21 is a positive regulator in response to drought stress in maize.

The xylem of anisohydric Quercus alba L. is more vulnerable to embolism than isohydric codominants

  • Pages: 329-346
  • First Published: 13 December 2021
Summary Statement

Across 10 diverse eastern US stands, anisohydric Quercus alba possessed more embolism-vulnerable stems than isohydric Acer saccharum and Liriodendron tulipifera. Although oaks are often considered drought-tolerant species, Q. alba sustains gas exchange by operating with heightened risk for hydraulic dysfunction.

Spray-induced gene silencing targeting a glutathione S-transferase gene improves resilience to drought in grapevine

  • Pages: 347-361
  • First Published: 19 November 2021
Summary Statement

Here, we used the innovative SIGS technique to functionally characterize a glutathione-S-transferase (VvGST40) gene in grapevine which indeed showed an improved resilience when submitted to severe water stress treatment.

Open Access

Genotype-dependent contribution of CBF transcription factors to long-term acclimation to high light and cool temperature

  • Pages: 392-411
  • First Published: 19 November 2021
Summary Statement

Long-term maintenance of photosynthetic upregulation was independent and freezing tolerance was partly independent of CBF1–3 in an Arabidopsis thaliana ecotype from a cold climate. This is contrasted with the greater reliance on CBF1–3 for long-term acclimation in an ecotype from a warmer climate.

Global identification of full-length cassava lncRNAs unveils the role of cold-responsive intergenic lncRNA 1 in cold stress response

  • Pages: 412-426
  • First Published: 01 December 2021
Summary Statement

  • A total of 3004 full-length lncRNAs in cassava under normal or cold-treated conditions were identified from Iso-seq data.

  • CRIR1 plays a positive role in plant response to cold stress by modulating the expression of stress-responsive genes and increasing their translational yield.

Open Access

Norway spruce deploys tissue-specific responses during acclimation to cold

  • Pages: 427-445
  • First Published: 07 December 2021
Summary Statement

Norway spruce shares components with the cold regulon previously described in the herbaceous annual plant model Arabidopsis, but has delayed responses and previously undescribed tissue-specific regulatory components that contribute to the extreme cold tolerance of this long-lived, evergreen coniferous species.

Open Access

Nucleus and chloroplast: A necessary understanding to overcome heat stress in Pinus radiata

  • Pages: 446-458
  • First Published: 02 December 2021
Summary Statement

Here, we show how nuclei and chloroplasts exchange signals and coordinate their proteomes to overcome heat stress in Pinus radiata. The acclimation process is reflected in a profound physiological rearrangement and a reprogramming of RNA metabolism including an increase in microRNA contents.

Solanaceous plants switch to cytokinin-mediated immunity against Ralstonia solanacearum under high temperature and high humidity

  • Pages: 459-478
  • First Published: 14 November 2021
Summary Statement

Upon Ralstonia solanacearum infection, pepper activates SA and JA immunities in its roots under ambient temperature, but these immunities are repressed, instead, a cytokinin-mediated immunity linked to a subset of Glutathione S-transferases is activated under high temperature and high humidity.

Open Access

cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis

  • Pages: 479-495
  • First Published: 14 November 2021
Summary Statement

The accumulation of Dolichols in Arabidopsis is affected by the cis-prenyltransferase 3 (CPT3) and an α/β-hydrolase (ABH). Using QTL and GWAS approaches we have identified these two and several other candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols.

Open Access

Strigolactones: New players in the nitrogen–phosphorus signalling interplay

  • Pages: 512-527
  • First Published: 31 October 2021

The present work shows that plants prioritize N over Pi limitation by affecting strigolactone (SL) biosynthesis. Moreover, we show that SLs play a key role acting as sensors during early plant responses to both N and Pi deficiency by regulating the expression of key regulatory genes of both signalling pathways and mediating in the N–P signalling interplay.

Defence-related pathways, phytohormones and primary metabolism are key players in kiwifruit plant tolerance to Pseudomonas syringae pv. actinidiae

  • Pages: 528-541
  • First Published: 13 November 2021
Summary Statement

The pandemic bacterium P. syringae pv. actinidiae is currently the most important pathogen affecting kiwifruit productivity worldwide. Given the absence of sustainable and effective mitigation strategies, it is imperative to understand plant tolerance mechanisms that could support crop improvement and better agronomical practices. Here, we found that plant susceptibility to Psa results from an inefficient activation of plant defences, involving the jasmonic acid and salicylic acid pathways, and leads to impairments in primary metabolism, particularly the ammonia assimilation cycle. Tolerance is most likely due to a more strategic defensive and metabolic readjustment, resulting from the activation of specific defence-related genes and to the downregulation of the abscisic acid pathway.

Open Access

A conserved clathrin-coated vesicle component, OsSCYL2, regulates plant innate immunity in rice

  • Pages: 542-555
  • First Published: 06 December 2021
Summary Statement

This study shows that OsSCYL2, a eukaryotic conserved SCY1-LIKE kinase, interacts with the medium subunit μ1 of clathrin-coated adaptor protein complex 1 (OsSPL28) via clathrin heavy chain 1, and regulates plant immunity and cell death in rice. These results shed new insights into the clathrin-mediated vesicle trafficking-mediated signalling pathway in the regulation of plant immunity, and therefore may provide new breeding targets to achieve both high yield and enhanced resistance in crops.

Insights into ROS-dependent signalling underlying transcriptomic plant responses to the herbicide 2,4-D

  • Pages: 572-590
  • First Published: 20 November 2021
Summary Statement

2,4-D induced a reactive oxygen species (ROS)-related peroxisomal footprint in early plant responses. ACX1 is one of the main sources of ROS production and associated epinasty phenotype following 2,4-D application in plants. ACX1-dependent signalling in plant responses to 2,4-D point to protein degradation associated with auxin signalling at the early response and with ubiquitin E3-RING and E3-SCF-FBOX/proteasome as key processes in the development of epinasty over longer treatment periods.